Objective To analyze the clinical characters of the different degrees nonalcoholic fatty liver (NAFLD) and the relationships with blood uric acid.Methods Two hundred and eighty-two cases body examinational and hospitalized patients were selected.According to whether complicated with NAFLD and liver steatosis degree,patients were divided into 4 Groups.Group A was control group that without NAFLD,Group B was mild NAFLD group,Group C was moderate NAFLD group and Group D was severe NAFLD group.Clinical data,blood lipids,blood uric acid and other biochemical indicators of four groups were compared.Results In 282 cases of patients,there were 78 cases (27.66％) without NAFLD patients,94 cases (33.33％) mild NAFLD,92 cases (32.62％) moderate NAFLD and 18 cases (6.38％) severe NAFLD.As the severity of fatty liver increased,body mass index (BMI) higher and higher ((24.51 ± 2.55),(27.06± 2.81),(28.37± 3.23),(30.71 ± 2.96) kg/m2),and the differences among four groups were statistically significant (F =35.976,P ＜0.05).Gglycerin trilaurate (TG),low density lipoprotein cholesterol (LDL-C) of D group were hihger than other groups ((3.04± 1.22) mmol/L vs.(1.73± 1.46),(2.59 ± 1.78),(2.81 ± 1.94) mmol/L,(2.99± 0.52) mmol/L vs.(2.28±0.78),(2.47±0.75),(2.41±0.74) mmol/L),high density lipoprotein cholesterol (HDL-C) was lower compared with group A,B,C (1.00± 0.23) mmol/L vs.(1.15± 0.24),(1.05± 0.21),(1.00 ±0.21) mmol/L),the differences were statistically significant(F=6.818,4.597,6.414;P＜0.05).In 4 groups the blood uric acid(UA) of groups B,C,D was higher than group A,group D was higher than group B,C,and the differences were statistically significant((316.13±71.86),(356.98±83.00),(355.37±81.01),(409.67 ±50.90) μmol/L;F=8.797;P＜0.05).Conclnsion Obese patients are more susceptible to NAFLD,and NAFLD with a certain degree of lipid and uric acid metabolism,and the severity of NAFLD has a positive correlations with lipids and serum uric acid levels.It is particularly obvious in patients with severe NAFLD,which prompting that blood uric acid level can be used as an independent risk factor in the pathogenesis of NAFLD,and as an auxiliary judge for NAFLD severity index.

BACKGROUND: Pathological changes of form and structure are induced in some tissues during repairing albuginea penis trauma, while structure and component of collagen fiber will be correspondingly changed necessarily.OBJECTIVE: To find a convenient, shortcut, and sensitive method to observe the dynamic change of type and distribution of collagen fiber during albuginea penis healing. DESIGN, TIME AND SETTING: A contrast study was performed at the Laboratory of Tissue Engineering, Guiyang Medical College between June 2005 and October 2007. MATERIALS: Fifteen male New Zealand rabbits were used in this study. METHODS: All rabbits underwent an excision of albuginea sizing 5 mm×5 mm at dorsal penis to establish models of albuginea penis trauma, and then the excision was sutured in situ. Thereafter, albuginea penis was obtained from five rabbits once after 2, 6, and 12 weeks. The samples were treated with picric-sirius red staining and observed under polarizing microscope. MAIN OUTCOME MEASURES: Dynamic changes of area of collagen fiber during albuginea penis healing.RESULTS: There were significant differences in content of the same collagen fiber at different time points (P<0.05). At 2 weeks post-trauma, both type Ⅰ and type Ⅲ collagen fibers were observed, and the ratio was equal, but the arrangement was confused and disorder. At 6 weeks post-trauma, red and yellow type Ⅰ collagen fiber was apparently increased, but type Ⅲ collagen fiber was decreased, and the arrangement was still disorder. At 12 weeks post-trauma, thick, bright red and yellow type Ⅰ collagen fiber was rapidly increased, but thin and green type Ⅲ collagen fiber was rapidly decreased.CONCLUSION: Picric-sirius red polarized light method can not only differentiate types of collagen fiber, but also clearly display the distribution, form and proportion of type Ⅰ and type Ⅲ collagen fiber, characterizing by simple, convenient, specific, and highly sensitive.

Adolescent ; Adult ; Aged ; Cystitis ; etiology ; prevention & control ; Female ; Hematopoietic Stem Cell Transplantation ; adverse effects ; Hemorrhage ; etiology ; prevention & control ; Humans ; Injections, Intravenous ; Male ; Mesna ; administration & dosage ; therapeutic use ; Middle Aged ; Transplantation Conditioning ; methods ; Young Adult

Humans ; Hypertension, Pulmonary ; diagnosis

Objective to investigate the correlation between glutathione S- transferase gene M1,t1 and P1(GStM1,GStt1 and GStP1)poly-morphism and sperm DNA fragmentation index(DFI)in male patients with idiopathic infertile. Methods the study included 246 male patients with idiopathic infertility. Polymerase chain reaction(PCR)and polymerase chain reaction restriction fragment length polymorphism(PCR-RFLP) were used to identify the genotype of GStM1,GStt1 and GStP1,respectively. Sperm DFI was analyzed by flow cytometry with acridine orange staining. Results the sperm DFI was significantly higher in GStt1 gene null type group than in GStt1 gene wild type group(P < 0.01);and there were no significant differences in GStM1 and GStP1 gene mutation type groups when comparing with the wild type group(P > 0.05). Conclusion GStt1 gene deletion was positive associated with the sperm DFI in male idiopathic infertile patients.

Objective To evaluate the efficacy of combination therapy with M and α receptors antagonist for the treatment of double-J stent related lower urinary tract symptoms.Methods From May 2013 to May 2014,131 cases,including 71 male and 60 female cases,were accepted the doubte-J stent indwelling after the ureteral lithotripsy,laparoscopic ureterlithotomy and pyeloureteroplasty.Their data was retrospectively reviewed.The age ranged from 29 to 64 years old,mean (47.4 ± 15.2) years old.They were divided into 4 groups randomly,including group A (control group,n =30),no drugs were taken;group B (tamsulosin group,n =34),0.2 mg tamsulosin was taken qd;group C (solifenacin group,n =32),5 mg solifenacin was taken qd;group D (tamsulosin combined with solifenacin group,n =35),0.2 mg tamsulosin and 5mg solifenacin were taken qd.The IPSS scores,QOL scores and visual analogue pain scale (VAPS) scores were assessed pre-operation,1 week after operation,and 4 weeks after operation,respectively.Results All patients were followed-up until the end of this study.In each time point,the IPSS scores in group A was 9.01 ± 2.79,13.18 ± 3.79 and 13.79 ± 3.76,respectively.In group B,the IPSS scores were 7.89 ± 4.29,12.39 ±3.90 and 12.21 ±3.87,respectively.In group C,the IPSS scores were 7.94 ±4.27,12.70 ±4.01 and 11.98 ±4.69,respectively.In group D,the IPSS scores were 8.21 ±3.18,11.97 ±5.03 and 8.17 ± 3.25,respectively.Significant difference in total IPSS scores and obstruction symptom scores were shown between pre-and post-operation (P ＜ 0.05).Comparing to other groups,group D exhibited the significant improvement in IPSS scores 1 and 4 weeks after the operation (P ＜0.05).4 weeks after operation,the QOL scores in group D was significantly lower than that in other groups (P ＜ 0.05).While the VAPS scores didn t show significant differences among those groups (P ＞ 0.05).Conclusion M and α receptors antagonist combination therapy can significantly improve lower urinary tract symptom due to indwelling double J stents.

The pathogenesis of male infertility involves the interactions between environmental and genetic factors. An individual's susceptibility to male infertility is influenced by his internal abilities of metabolizing and detoxicating endogenous and exogenous chemicals. Glutathione-S-transferases (GSTs), such as enzymes, are involved in the cellular detoxication of various physiological and xenobiotic substances. Studies show that the polymorphism of the GSTs gene is correlated with male infertility. GSTs polymorphism-related susceptibilities to male infertility were found similar but with some inconsistencies within the same community, and inconsistent with some similarities among different communities. Therefore, further studies are to be done on the differences in GSTs polymorphism-related susceptibility to male infertility in different communities.
Genetic Predisposition to Disease ; Genotype ; Glutathione Transferase ; genetics ; Humans ; Infertility, Male ; genetics ; Male ; Polymorphism, Genetic

OBJECTIVETo explore gender difference of relationship between occupational stress and depressive symptoms.

METHODSThe cross-sectional study was used to investigate 5338 workers in 13 factories and companies, the depressive symptoms were assessed with Epidemiologic Studies Depression Scale (CES-D) and the perceived occupational stress was evaluated by the Chinese version of the Job Content Questionnaire (JCQ) and Effort-Reward Imbalance (ERI) Model Questionnaire. Logistic regression analyses were conducted to estimate the associations between occupational stress and prevalence of depressive symptoms.

RESULTSPrevalence of depressive symptoms was 31.8% for all subjects, 33.8% for males and 27.7% for females, gender difference of prevalence was significant (P < 0.01). Psychological demands, physical demands, job control, effort, overcommitment and negative affectivity scores for males were significantly higher than those for females(P < 0.01 or 0.05), social support, reward and job satisfaction scores for males were significantly lower than those for females (P < 0.01). Psychological demands, physical demands, job control, effort, overcommitment and negative affectivity scores for workers with depressive symptoms were significantly higher than those for workers without depressive symptoms (P < 0.01) but job control, social support, reward, job satisfaction and positive affectivity scores for workers with depressive symptoms were significantly lower than those for without depressive symptoms (P < 0.01). Psychological demands, physical demands, job control, effort, overcommitment and negative affectivity scores for male workers with depressive symptoms were significantly higher than those for female counterparts (P < 0.01), but social support, reward, job satisfaction scores for male workers with depressive symptoms were significantly lower than hose for female workers with depressive symptoms (P < 0.01). Ratio of male workers and male workers with depressive symptoms with job strain and ERI > 1, high demands and low control, and high effort and low reward were higher than female counterparts (P < 0.01). The risk of depressive symptoms for female with high demands and low control was about two times as high as that for female workers with low demands and high control for psychological demands and physical demands (OR = 2.0), risk for females was slightly higher than that for males (OR = 2.04 and 2.17, respectively). The risk of depressive symptoms for workers with high efforts and low rewards was about two times as high as that for workers with low efforts and high rewards (OR = 1.70). No interactive effect between gender and job strain and ERI on depressive symptoms were found.

CONCLUSIONThe gender difference of relationship between depressive symptom prevalence and job strain may due to the difference of psychosocial factors between females and males. There may be no interactive effect between psychosocial factors and gender on depressive symptoms.

Adolescent ; Adult ; Cross-Sectional Studies ; Depression ; epidemiology ; Female ; Humans ; Job Satisfaction ; Male ; Middle Aged ; Sex Factors ; Stress, Psychological ; epidemiology ; Surveys and Questionnaires ; Young Adult

PurposeTo study the effect of gasoline on the extracellular matrix of dermis in rat. Methods45 male Wistar rats were divided into control group, 3 different exposure level groups, which were exposed to gasoline on 2 cm× 3 cm skin area with the dose of 250 mg/cm2 for 1 (group A) ,4(group B)and 8 days (group C) respectively,and an intervention group exposed to 250 mg/cm2 × 8 days gasoline after the application of protective agent on skin. After the treatment, collagen, elastin and glycosaminoglycan (GAG) were assayed in skin departed from intoxicated area.ResultsCompared with control group, collagen was decreased in group C(P<0.05); elastin was decreased in group B and C (P<0.05); glycosaminoglycan was decreased in all exposure groups and intervention group ( P < 0.05 or P < 0.01 )。 Collagen, elastin and glycosaminoglycan(GAG) were significantly lower in group C than in intervention group ( P < 0.05 ). ConclusionsThe extracellular matrix of dermis,including collagen,elastin and GAG were decreased in rat dermal exposed to gasoline.

Objective To investigate the typical value of gamma-radiation dose rate and its confidence interval in tank-transported copper ore by using bootstrap resampling techniques. Methods Bootstrap resampling method, coupled with kernel density estimation, introduced to acquire the typical value of gamma-radiation dose rate in copper ore. Results The typical value of gamma-radiation dose rate in copper ore was expressed as the central tendency of the means of resampling, and two kinds of confidence interval, empirical percentile and bias-corrected accelerated confidence interval, were provided as standard error. Conclusion It is clearly demonstrated that this method has an advantage to give a robust description in explanation of central tendency and variation range of gamma-radiation dose rate data profiles.