Adult ; Carcinoma, Hepatocellular ; secondary ; Humans ; Liver Neoplasms ; secondary ; Lymphoma, Non-Hodgkin ; pathology ; Male ; Spinal Neoplasms ; pathology

Acupuncture Therapy ; Female ; Humans ; Middle Aged ; Paralyses, Familial Periodic ; therapy

Objective To evaluate the effects of celecoxib on tumor growth,COX-2 and survivin expressions and angiogenesis in nude mice. Methods Xenograft animal model was established by injecting human colon cancer HT-29 cells into the BALB/c nude mice subcutaneously. Fifty mice were randomly divided into 4 groups 16 d after injection:control group,celecoxib group(receiving 25,50,75,100 mg?kg-1?d-1 for 35 d). Tumor volumes were measured every week. The expression level of COX-2,survivin and the microvessel density (MVD) of the xenograft tumor tissues were measured by immunohistochemistry,and mRNA level of VEGF by RT-PCR. Results Celecoxib at dose of 25,50,75 and 100 mg?kg-1?d-1 inhibited the tumor volume by 34.94%,39.20%,53.50%,59.20% respectively,and showed more effective in suppressing the tumor growth than the control group(P

BACKGROUND:It needs strong anchorage for shifting mandibular molar in orthodontics,which is a difficult problem to clinical orthodontic doctors.OBJECTIVE:To evaluate clinical effects and the characters by micro-implant anchorage during mandibular molars mesialization in Class Ⅰ malocclusal patients.METHODS:A total of 24 micro-implants were embedded mandibular bones between mandibular second premolar and mandibular first premolar of 15 Angle Ⅰ malocclusal patients as clinical anchorages for mesializing mandibular molars.The position changes of mandibular molars were measured from mesiodistal direction and vertical direction,and the implant anchorage loss was evaluated by maxillary central incisor.RESULTS AND CONCLUSION:The course of treatment was 10.4 months,and the velocity of mandibular second molar mesializing was 0.8 mm per month,with 8.5 mm in mesiodistal direction,there was no changes in the vertical direction.The distal tipping angle of molar was 2.5°,and the mandibular central incisor did not move.The method successfully mesialized mandibular molars to appropriate positions.No anchorage loss was found,The implant plays absolute anchorage during mandibular molar mesialization.

This research is to establish an HPLC method for the simultaneous determination of ophiopogonin D, ophiopogonin D', ophiopogonin C, deacetylophiopojaponin A and ophiogenin-3-O-α-L-rhamnosyl-(1-->2)-β-D-glucoside in Ophiopogonis Radix. HPLC-ELSD analysis was performed on a Kromasil 100-5 C₁₈ column (4.6 mm x 250 mm, 5 µm), with the mobile phase of acetonitrile (A) -water (B) in gradient elution mode (0-45 min, 35%-55% A), at a flow rate of 1 mL · min⁻¹. The column temperature was 35 °C and the drift tube temperature was 100 °C in a gas flow rate of 3.0 L · min⁻¹. The result showed that baseline of all the 5 constituents was well separated, and every constituent had wide linearity range and good linear relation (r > 0.999). The recovery rate was between 95.75% and 103.1%. The new established method for simultaneous determination of saponin constituents in Ophiopogonis Radix was sensitive and has good, repeatability. It could be applied to quality evaluation of Ophiopogonis Radix.
Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Ophiopogon ; chemistry ; Plant Roots ; chemistry ; Saponins ; chemistry ; isolation & purification

Objective To investigate the typical value of gamma-radiation dose rate and its confidence interval in tank-transported copper ore by using bootstrap resampling techniques. Methods Bootstrap resampling method, coupled with kernel density estimation, introduced to acquire the typical value of gamma-radiation dose rate in copper ore. Results The typical value of gamma-radiation dose rate in copper ore was expressed as the central tendency of the means of resampling, and two kinds of confidence interval, empirical percentile and bias-corrected accelerated confidence interval, were provided as standard error. Conclusion It is clearly demonstrated that this method has an advantage to give a robust description in explanation of central tendency and variation range of gamma-radiation dose rate data profiles.

OBJECTIVETo establish a method for determining the content of caffeic acid in compound Yinhuangjiedu decoction using high-performance capillary electrophoresis (HPCE).

METHODSThe optimized HPCE for determining caffeic acid content utilized a fused-silica capillary tube (75 microm x 60 cm, effective length of 53 cm) with 20 mmol/L borate as the running buffer (pH=9.18), a constant voltage of 12 kV, a sampling time of 5 s at 25 degrees celsius, and UV detection wavelength at 313 nm.

RESULTSThe linear range of caffeic acid was 20-100 microg/ml.

CONCLUSIONHPCE is simple, rapid, and sensitive for quality control of the compound Yinhuangjiedu decoction.

Caffeic Acids ; analysis ; Drugs, Chinese Herbal ; chemistry ; Electrophoresis, Capillary ; methods ; Medicine, Chinese Traditional

Objective To elucidate the possible role of p53-p21waf1 pathway for centrosome amplification in oral squamous cell carcinoma ( OSCC ) . Methods Formalin-fixed, paraffin-embedded tissues of 8 cases of normal oral epithelium tissues and 27 cases of OSCC tissues were examined for the expression of p21waf1 and mutated p53 proteins by flowcytometry and immunohistochemistry, and centrosome status was investigated by indirect immunofluorescence double staining with antibodies to centrosome protein gamma-tubulin and cytokeratin. The correlation between p21waf1, p53 and centrosome amplification in OSCC was statistically analyzed by SPSS 12.0. Results All normal oral epithelium tissues showed normal centrosomes( 1-2 centrosomes per cell) in epithelium cells, while 21 out of 27 cases(78%) of OSCC showed the evidence of centrosome amplification characterized by supernumerary centrosomes (＞2 centrosomes per cell) in a fraction of tumor cells. The quantity of p21waf1 protein was lower in OSCC with centrosome amplification [(0. 878±0. 081 )] than that in OSCC without centrosome amplification [ (0. 952±0. 018 ) ,t =3.838,P<0.01] , and negative correlations were found between the quantity of p21waf1 protein and the degree of centrosome amplification ( r = - 0.472, P＜0. 05) , as well as the positive staining of p53 ( r =-0.491 ,P＜0. 01). Conclusions p53-p21waf1 pathway might involve in centrosome duplication cycle in OSCC. Down-regulated p21waf1 protein, via p53 transactivation-dependent mechanism, was likely a contributing factor towards centrosome amplification in OSCC.

OBJECTIVETo establish a method for detecting plasma polydatin using high-performance liquid chromatography (HPLC) and investigate the pharmacokinetics of polydatin in Beagle dogs.

METHODSHPLC-based detection of polydatin was performed on a Hypersil-BDS C18 column with a mobile phase of methanol and water (35:65) at the flow rate of 1.0 ml/min and the detection wavelength of 290 nm. The pharmacokinetic parameters of polydatin were calculated by non-compartment model statistics.

RESULTSThe standard curve was linear within the range of 0.21-21.0 microg/ml (correlation coefficient of 0.9995, n=5). The average recovery was 90% with a relative standard deviation no more than 8.0%. The limit of detection of the method was 0.1 microg/ml. The pharmacokinetic parameters of polydatin at 3 doses were obtained, with T((1/2)); of 89.95-/+19.96 min, 152.15-/+55.82 min, and 202.36-/+66.75 min, and AUC0-infinity of 300.14-/+51.33 g.min.ml(-1), 751.72-/+173.97 g.min.ml(-1) and 1521.12-/+310.02 microg.min.ml(-1), respectively.

CONCLUSIONThe method we established allows effective detection of polydatin, whose pharmacokinetics conforms to the two-compartment model.

Animals ; Chromatography, High Pressure Liquid ; Dogs ; Female ; Glucosides ; blood ; pharmacokinetics ; Limit of Detection ; Linear Models ; Male ; Reproducibility of Results ; Stilbenes ; blood ; pharmacokinetics

A bacterial strain DN25, effective on cyanide-degradation, was isolated from contaminated soil and identified as Alcaligenes sp. on the basis of phenotype analysis and 16S rDNA sequence analysis. It showed great tolerance to the cyanide, which can grow in the medium containing 500mg CN -/L. The suitable condition for the cell growth and boitransformation was pH8.0 and 30oC and the transformation rate for 500mg CN - /L could achieve 99% in 10 h. It has also been found that the screened strain had the ability of K 4Fe(CN) 6 transformation with 96% of transformation rate at 12 h for the concentration of 500 mg CN /L.