The change in the venous concentration of amino acids in normal male and female adults was studied. After the brief fast (about 40 hours) except for a higher concentration of branched-chain amino acids in the plasma of female subjects, there was essentially no significant difference between the plasma composition of amino acids in female or male subjects. In only one male subject (Rohler's Index was 106), the transient fall of the branched-chain amino acid concentrations.
Low caloric diet feedings resulted in a marked reductions of the plasma serine, glutamic acid, proline, glycine, arginine and threonine. On the other hand, high protein and high fat diet feedings resulted is a remarkable increasing of the branched-chain amino acid concentration and marked reduction of the plasma alanine concentration.

Before and after the physical fitness tests included 1000 meter endurable walking, aged males and females more than 61 to 80 years old were tested on the blood pressure, heart rate and blood components. The results were as follows.
1) The systolic and diastolic blood pressure were not varied so distinctly.
2) Heart rates were increased in all groups.
3) Serum cholesterol, GOT and GPT were not influenced.
4) Fatty acid, especially unsaturated one, was increased remarkably.
5) Fatty acid increasing observed on excellent performer of the endurable walking was higher than that of poor performer.
6) Increasing ratio of the fatty acid which was found on the subjects with abnormal ECG was higher than that of the subjects with normal ECG.
As above mentioned facts, the test of serum fatty acid seemed very important item to the aged people from the view point of security of the exercise.

The remarkable increase of branched amino acid and pretty decrease of lysine venous concentration were observed in healthy nine male and female adults by the brief fast.
The increase of branched amino acid wasn't able to find by the various exercise generally. The increase of lysine venous concentration was found after the running more than 10km frequently.
Only on the subjects of 44 and 55 years of age, the increase of branched chain amino acid and lysine were observed by the running. It looks like have to take precautions that aged people run under the conditions of empty stomach.

Venous concentration of lysine and other 16 amino acids were determined in healthy 5 male 20-22 years of age subjects in the resting state, after 2 min from bigining of exercise, immediatetly after all-out exercise and 30 min after of the exercise with a bicycle ergometer at work intensities which will be fell into all-out in about 6 or 7 min.
During 2 min exercise lysine only was increased, no change of glycine and puoline were observed and other amino acids were decreased. At the point of all out increase of threonine, lysine and histidine were observed, compared with resting state
Alanine concentration at 30 min after of all out exercise was very high than that of resting state and same tendency but a little was observed on lysine. Other amino acids were decreased.
A risk connected with exercise was discussed from the data of lysine obserbed high concentration during two min exercise concerning with the variation of other amino acids by the all-out exercise.

Evidence of an increased resting blood flow in the tendon against the adjacent muscle was confirmed in the in situ hindlimb preparation of a rabbit anesthetized with urethane. The tendon tissue blood flow was found to be modulated by nervous and mechanical factors. The effect of nervous control was demonstrated by the denervation of the sciatic nerve, which showed a gradual increase in the resting blood flow in the tendon and muscle after being severed. During local muscle exercise, the increase of tendon tissue blood flow observed was minimal in comparison with the adjacent muscle. The tendon surrounding tissue blood flow showed site-dependent characteristics along the longitudinal tendon bundles. Evidence of tendon vascular structure in the rabbit species was confirmed by observing capillaries in cross sectional fascicles and longitudinal and transversal vessels in the paratenon of the Achilles tendon. These results strongly suggest a physiological model of local tissue temperature regulation and fluid dynamics in the biological system.