Objective: To establish the detemination method of bismuth subcarbonate in Weishu Powder. Methods: The samples were ignited, then bismuth subcarbonate in residue was determined by compleximetry. Results: The average recovery was 99.36?0.14%(n=9). Conclusion: This method can be used in quality control of Weishu Powder preparation.

AIM:To investigate the effects of down-regulated miR-9 expression on the proliferation , invasion and migration of nasopharyngeal carcinoma (NPC) cells.METHODS:Human NPC CNE1 and CNE2 cells were transfect-ed with the inhibitor of miR-9 by Lipofectamine to down-regulate the expression of miR-9, and the cells transfected with an inhibitor control were also set up .The cell proliferation and cell cycle were evaluated by CCK-8 assay and flow cytometry . The cell invasion and migration abilities were detected by Transwell invasion and wound -healing assays .Immunoblotting was applied to analyze the levels of the proteins .RESULTS:Compared with control group , inhibition of miR-9 expression in the NPC cells by transfection of the miR-9 inhibitor significantly decreased the proliferation ability (P<0.05).The per-centages of the cells in G 0/G1 phase [ CNE2: ( 57.96 ±1.39 )% vs ( 47.93 ±1.76 )%, P<0.05; CNE1: ( 51.24 ± 0.88)%vs (48.29 ±0.39)%, P<0.05] were significantly increased.The migration distances [CNE2: (186.50 ± 7.94)μm vs (247.56 ±15.56)μm, P<0.05;CNE1:(139.06 ±16.73)μm vs (230.66 ±14.27)μm, P<0.01] and the invasion ability of the CNE2 cells (43.00 ±3.17 vs 65.80 ±5.20, P<0.01) were also significantly inhibited .Moreo-ver, the tumor cells transfected with the inhibitors produced lower β-catenin.CONCLUSION:Inhibition of miR-9 expres-sion suppresses the proliferation , invasion and migration of nasopharyngeal carcinoma cells .

To analyze influenza pathogen spectrum in Yunnan province during 2009-2014 years, and analyze HA and NA genes of influenza A H1N1. Analysis was made on the monitoring date of influenza cases in Yunnan province in recent 6 years, 23 strains of influenza virus of HA and NA gene was sequenced and analyzed by MEGA 5 software to construct phylogenetic tree. 4 times of influenza AH1N1 epidemic peak were monitored from 2009-2014 years in Yunnan Province, as the nucleic acid detection results of influenza A H1N1 accounted for 28.8% of the total. The sequencing result showed that HA and NA gene were divided into 3 groups, one was detected with H275Y mutation strains. Influenza A H1N1 is one of the important subtypes in Yunnan province and their genes have divided into three branches during the period of 2009-2014 years, the vast majority of influenza a H1N1 are still sensitive to neuraminidase inhibitors.
China ; epidemiology ; Hemagglutinin Glycoproteins, Influenza Virus ; genetics ; metabolism ; Humans ; Influenza A Virus, H1N1 Subtype ; classification ; enzymology ; genetics ; isolation & purification ; Influenza, Human ; epidemiology ; virology ; Molecular Sequence Data ; Mutation ; Neuraminidase ; genetics ; metabolism ; Phylogeny ; Viral Proteins ; genetics ; metabolism

Objective To understand the viral etiology of acute respiratory infection in Kunming area. Methods We collected the nasopharyngeal swab of patients with acute respiratory tract infection,and used multiple reverse transcription-polymerase chain reaction (RT-PCR) method to detect 15 kinds of respiratory viral pathogens. Results Among the 600 samples,144 strains of viruses were detected, the positive rate was 24%,among which the highest positive rate was RSV (49/600,8.2%),followed by PIV (32/600,5.3%) HRV (27/600,4.5%) and IFV27 (27/600,4.5%) . The respiratory virus infection situation was different in every age group, groups of the highest virus positive rate was ≤1 age group (72/216, 33.3%);The respiratory virus infection situation in different seasons was different, the virus positive rate of the first quarter was the highest (85/144, 59%) . Conclusion RSV was the main virus pathogen of acute respiratory tract infections in Kunming area in 2011 years, the detection rate in sick children was the highest among all patients;the detection rate in the first quarter was higher than other quarters.

Objective To investigate the correlation between early blood pressure variability and early neurological deterioration (END) in patients with acute ischemic stroke.Methods Inpatients with acute ischemic stroke were collected prospectively.The blood pressure values of the enrolled patients were recorded continuously for 72 h after admission.The mean value (mean),maximum value (max),differences betw een the maximum and minimum (max-min),standard deviation (SD),and coefficient of variation (CV) for the systolic blood pressure (SBP) and diastolic blood pressure (DBP) were calculated.END was as an increase of at least 2 in the highest score of the National Institute of Health Stroke Scale (NIHSS) compared to the baseline.Multivariate logistic regression analysis was used to identify the independent correlation between the different blood pressure variability parameters and END following acute ischemic stroke after adjusting the confounding factors.Results A total of 128 patients with acute ischemic stroke were enrolled,including 53 females and 75 males,and their mean age was 63.30 ± 11.82 years.After standard treatment,35 patients (27.34％) developed END within 72 h after admission.There were significant differences in age,sex,diabetes mellitus,baseline NIHSS,C-reactive protein and SBPmax,SBP in,SBPSD,SBPCv,DBPmax,DBP max-min,DBPsD,and DBPCv between END group and non-END group (all P ＜0.05).Multivariate logisticregression analysis showed that SBPmax-min(odds ratio [OR] 1.040,95％ confidence interval [CI] 1.014-1.067,SBPsD(OR 1.191,95％ CI 1.052-1.347),SBPCv(OR 1.317,95％ CI 1.100-1.578),DBP max-min(OR 1.076,95％ CI 1.018-1.138),DBPsD(OR 1.508,95％ CI 1.128-2.016),and DBPCv(OR 1.338,95％ CI 1.093-1.638) in blood pressure variability indices were the independent risk factors for END in patients with acute ischemic stroke.Conclusion Blood pressure variability is significantly associated with END within 72 h after admission in patients with acute ischemic stroke.

With the development of economy,increase of cultural exchanges and changes of people's ideology in China,the number of female sex workers (FSWs) increased rapidly under the influence of various social factors.The diverse motivations for women engaging in commercial sex have been observed.Foreign researchers have conducted some surveys of factors associated with female commercial sex,while few such studies were conducted among FSWs in China.This paper summarizes the progress in the research of reasons for women engaging in commercial sex both at home and abroad to provide evidence for future study.

Objective To understand the gender characteristics,HIV/AIDS related knowledge awareness and behaviors of transgender women.Methods A questionnaire survey was conducted among the transgender women recruited through snowball sampling in Jinan in 2014,and descriptive epidemiologic analysis was conducted on the survey results.Results A total of 55 transgender women were surveyed,all of them were male physically and female psychologically.Serious gender conflict occurred in 27 subjects (49.1％),and very serious gender conflict occurred in 8 subjects (14.5％).Thirty subjects dressed up as a man in social life,accounting for 54.5％;25 subjects dressed up as a women in social life,accounting for 45.5％.The average awareness rate of HIV/AIDS related knowledge was 57.9％(22/38).The awareness of knowledge about AIDS associated behaviors,such as multi sex partner and anal sex,was poor.For the lovers or sexual partners,58.2％ of the subjects (32/55) would choose males and 50.9％ of the subjects (28/55) had chosen males,and for the sex partner at latest sex,63.6％(35/55) of the subjects had chosen males.Up to 56.3％ of the subjects had sex with casual sexual partners (net friends and partners of one-night stand) at latest sex behavior.Among the subjects surveyed,18(32.7％) never used condoms;29(52.7％) used condoms occasionally;4(7.3％) used condoms frequently and 4(7.3％) used condoms at each sex.Conclusions AIDS associated high risk behaviors were common among the transgender women,such as unprotected anal sex,multiple sexual partners,frequent sex and poor condom use.It is necessary to conduct the study of the HIV infection prevention in transgender women.

Objective:To compare the efficacy between drug-coated balloon (DCB) combined with bare metal stenting (BMS) and plain old balloon angioplasty (POBA) with BMS placement in the treatment of femoral-popliteal TASC D lesions.Methods:According to the Trans-Atlantic Inter-Society Consensus (TASC) D grade femoral-popliteal lesions as the standard, we enrolled 115 cases (120 limbs) receiving DCB combined with BMS (group DCB, 37 limbs in 36 cases) and POBA combined with BMS (group POBA, 83 limbs in 79 cases) from Jan 2017 to Mar 2020 to observe patency rate, freedom from clinical-drived target lesion reintervention rate (FCD-TLR) and complications.Results:The mean follow-up time was 18.1 months and the average occlusion length was (29.1±6.5)mm. In group DCB vs group POBA, the primary patency rates at 3-month, 9-month, 1-year and 2-year were 89.2% vs. 86.7%, 86.4% vs. 76.9%, 66.8% vs. 70.9% and 63.1% vs. 56.9%, respectively ( P=0.73); FCD-TLRs were 100.0% vs. 95.1%, 94.3% vs. 82.3% , 78.5% vs. 80.6% and 74.1% vs. 68.9% ( P=0.69), respectively. Conclusion:The benefit of DCB combined with BMS over POBA combined with BMS in improving the early primary patency rate and reducing FCD-TLR was not definite.

Objective:To evaluate the application value of three-dimension digital subtraction angiography (3D-DSA) in the diagnosis and treatment of iliac vein compression syndrome (IVCS).Methods:A retrospective analysis was made on 171 patients with a tentative diagnosis of IVCS based on signs, symptoms, and finding of CTV or MRV, and iliac vein angiography. The diagnostic efficacy of MRV, 2D-DSA and 3D-DSA were analyzed. The imaging advantages of 3D-DSA in the diagnosis and treatment of IVCS were evaluated.Results:Ninty-three patients underwent MRV and 3D-DSA simultaneously, 101 patients had 2D-DSA and 3D-DSA simultaneously. 3D-DSA was taken as gold standard, the diagonotic sensitivity, specificity, Youden index of MRV was 75.61%, 72.73% and 0.48 respectively. The sensitivity, specificity, Youden index of 2D-DSA was 90.22%, 100% and 0.90 respectively. There are significant differences in the diagonotic sensitivity between MRV and 3D-DSA, 2D-DSA and 3D-DSA ( P<0.05). There is no significant difference in the diagonotic specificity between MRV and 3D-DSA, 2D-DSA and 3D-DSA ( P=1.000). In this study, we found that 3D-DSA has greater imaging evaluation advantages in preoperative evaluation, intraoperative guidance and immediate postoperative reexamination in the diagnosis and treatment of iliac vein disease. Conclusions:3D-DSA can improve the detection rate of IVCS, and has its advantage in imaging evaluation.

［摘要］ 目的：探讨穿膜蛋白125（transmembrane protein125，TMEM125）在肺腺癌组织与A549 细胞中的表达，以及影响细胞 的增殖和侵袭能力的分子机制。方法：从癌症基因组图谱（the cancer genome atlas，TCGA）数据库收集肺腺癌数据包，下载临床 信息及基因表达谱数据。分析TMEM125 在肺腺癌组织中的表达及其与患者总生存期的相关性。构建TMEM125 过表达A549 细胞株，以CCK-8 法、细胞划痕实验检测TMEM125 过表达对肿瘤细胞的增殖和迁移能力的影响；流式细胞术检测TMEM125 过 表达对A549 细胞的细胞周期和凋亡的影响。WB检测TMEM125 过表达对下游NF-κB信号通路、凋亡蛋白的影响；免疫共沉淀 法（co-immunoprecipitation，Co-IP）检测TMEM125 与NF- κB 抑制因子结合Ras 样2（NF- κB inhibitor interacting Ras-like 2， NKIRAS2）的相互作用。利用TNFα（10 ng/ml）处理TMEM125 过表达A549 细胞，CKK-8、流式细胞术及WB检测其对细胞增殖、 凋亡以及NF-κB信号通路相关蛋白表达的影响。去甲基化试剂地西他滨处理A549 细胞，qPCR和WB检测TMEM125 基因和蛋 白的表达。结果：TMEM125 mRNA在肺腺癌组织中表达水平显著低于正常组织（P<0.001），启动子甲基化水平显著高于正常组 织（P<0.001），并且低、中表达患者总生存期显著低于高表达患者（P<0.001）。过表达TMEM125 抑制了A549 细胞的增殖和迁移 （P<0.01），增加细胞G2/M 期，促进细胞凋亡（P<0.01）；过表达TMEM125 与NKIRAS2 相互作用，显著抑制NF- κB 的活性 （P<0.01）；地西他滨处理A549 细胞可促进TMEM125 表达并且抑制细胞增殖（P<0.01）。结论：启动子高甲基化水平降低了 TMEM125 基因表达，导致其抑制NF-κB活性功能和抑制细胞增殖的作用下降，并且降低了细胞对地西他滨的敏感性。