Study on 475 patients with urealithiasis shows that 106 isolated strains are Enterobacteriaceae, 53 strains of them are E. coli (50%). The prevalence in woman is twice higher than this in men. All regular antibiotics were ineffective. Nofloxacin has high sensitivity
Urinary Tract Infections ; Escherichia coli

A study on 148 patients with urinary tract infection (UTI) in Hai Phong Children Hospital from January 2002 to 2004 got a result as follows: the infection rate was 45.3% in boys, and was 54.7% in girls; urologic malformation accounted for 6.1% and phimosis accounted for 9.5%. Positive urinary culture was 57.4% in which the most popular bacteria were E.coli (33.1%), Proteus (10.1%) and Enterobacter (6.1%). E.coli was sensitive to cefotaxim, nalidixic acid, gentamicin, amikacin and Augmentin but resistant to: cephalexin, ampicillin, choloramphenicol and co-trimoxazol. Preteus was sensitive to: Augmentin, ciprofloxacin, gentamicin, amikacin, cefotaxim, nalidixic acid and resistant to: ampicillin, chloramphenicol and co-trimoxazol. Enterobacter was sensitive to: cefotaxim, amikacin, ciprofloxacin, ceftriaxon and resistant to: gentamicin, ampicilin and chloramphenicol. General treatment results: recovered 54%, reduced 35.8%, and non-responded 10.2%. The average treatment duration for 3 groups was 8.685.03 days
Urinary Tract Infections ; Child ; Escherichia coli Infections

Enterohemorrhagic Escherichia coli ; Escherichia coli Infections ; epidemiology ; Germany ; epidemiology ; Humans

Africa ; epidemiology ; Escherichia coli Infections ; epidemiology ; Escherichia coli O157 ; Humans

Objective: To evaluate the typing and clinical application effect based on clustered regularly interspaced short palindromic repeats (CRISPRs), serotype, and Multilocus Sequence Typing (MLST). Methods: The spacers, serotype and sequence type (ST) were obtained with CRISPRsFinder, SeroTypeFinder and MLST. PCR was used to amplify the CRISPRs, and the spacers were used to predict serotype and ST, then comparing with the serotype and ST. Results: We defined the I-E CRISPR/Cas as CT-Ⅰ, I-F CRISPR/Cas as CT-Ⅱ, and only CRISPR3-4 as CT-Ⅲ. We designated each unique arrangement spacer profile as a unique CRISPRs type. A total of 79 CT types, 76 serotypes, and 66 STs were identified. The CRISPRs typing was the most discriminating, with the Simpson index of 0.936, having the highest correlation with serology with the adjusted Rand index of 0.908. The CRISPRs type could divide the same serotype (ST) into two subtypes [O157∶H7(ST11), O104∶H4(ST678), and O26∶H11(ST21)]. The detection rates of CRISPR1, CRISPR2, CRISPR3, CRISPR4, and CRISPR3-4 were 81.1%, 94.5%, 1.4%, 1.4%, and 4.6%, with the accuracy rate of 95.0% and 100.0% according to the spacers to forecast O157∶H7 (ST11) and ST131. Conclusion: Based on the CRISPRs spacer, this method can be used as an essential molecular typing for E.coli, as it presents a good typing and clinical application effect.
Escherichia coli/genetics* ; Escherichia coli Infections ; Humans ; Multilocus Sequence Typing

Nowadays the enteric infections are still the great concern over the world. According to the report from WHO, there is about 2.5 million deaths due to enteric infections every year in the developing countries: 120.000 deaths due to cholera, 380.000 deaths due to E.coli infections diarrhea excreting the toxin, 600.000 deaths due to dysentery, 600.000 deaths due to typhoid fever and 800.000 deaths due to rotavirus infectious diarrhea.
Immunization ; Escherichia coli Infections ; Typhoid Fever

No abstract available.
Escherichia coli* ; Escherichia* ; Humans ; Urinary Tract Infections* ; Urinary Tract*

No abstract available.
Escherichia coli* ; Escherichia* ; Urinary Tract Infections* ; Urinary Tract*

OBJECTIVETo investigate the etiologic value of diarrheagenic E. coil harboring genomic O island 28(OI-28) containing five putative virulence genes (Z0608, Z0609, Z0615, Z0634 and Z0635), which were related to RTX (Repeat in toxin) toxin family isolated from children with diarrheal disease in Taiyuan.

METHODSIn the study, 257 fecal samples from children with diarrheal disease collected in Shanxi Children's Hospital. Diarrheagenic E. coli and enteropathogenic bacteria were isolated and identified by conventional bacterial culture and typing specific diarrheagenic E. coli (EPEC, EIEC, ETEC and EHEC) diagnostic serum, while diarrheagenic E. coli harboring genomic 01-28 containing five putative virulence genes (Z0608, Z0609, Z0615, Z0634 and Z0635) were detected by PCR and DNA southern blot hybridization.

RESULTS206 strains (80.16%) of enteropathogenic bacteria were detected from 257 children with diarrhea disease, containing 149 strains (57.98%) of diarrheagenic E. coli and 57 strains(22.18%) of other entero-pathogenic bacteria. Among 3 strains (2.01%) of EPEC, 2 strains (1.34%) of ETEC, 2 strains (1.34%) EHEC were detected by typing specific serum, while all of the 142 strains (95.30%) isolated were suspected to be diarrheagenic E. coli. 21 strains (14.09%) of diarrheagenic E. coil harboring genomic O1-28 containing five putative virulence genes (Z0608, Z0609, Z0615, Z0634 and Z0635) were detected by polymerase chain reaction and DNA southen blot hybridization, 8 strains (5.37%) of diarrheagenic E. coli containing only one genomic OI-28 virulence gene, 2 strains (1.34%) of diarrheagenic E. coli containing two genomic OI-28 virulence gene. 21 children with diarrhea diseases caused OI-28-harboring E. coli containing five important putative virulence genes were among 0 to 3 years old (80.95%). These children correlating with OI-28-harboring E. coli did not present special clinical symptoms or signs.

CONCLUSIONThe diarrheagenic E. coil harboring genomic OI-28 was one of the important etiology for children with diarrheal disease in summer season.

Child ; China ; Diarrhea ; microbiology ; Escherichia coli ; genetics ; pathogenicity ; Escherichia coli Infections ; complications ; Genes, Bacterial ; Humans ; Virulence

A 29-year-old woman presented with bloody diarrhea, abdominal pain, hemolytic anemia, thrombocytopenia, and acute renal failure. She was diagnosed with Escherichia coli O104:H4-associated hemolytic-uremic syndrome (HUS) and treated with plasmapheresis and hemodialysis for 3 weeks. She recovered without sequelae. To the best of our knowledge, this is the first report of Escherichia coli O104:H4-associated HUS in Korea. We recommend that Escherichia coli O104:H4, as well as the more common O157:H7, be considered in the diagnosis of bloody diarrhea-associated HUS.
Humans ; Hemolytic-Uremic Syndrome/*microbiology ; Female ; Escherichia coli Infections/*complications ; Escherichia coli/*classification ; Adult