A study on the coefficient of the erythrocyte aggregation in the health person were:K60: 0.979 +/-0.0564; K90: 0.965+/- 0.073; K120: 0.956+/- 0.061. These coefficients in the patients with myocardial infarction were: K60:0.800+/- 0.083; K90:0.795+/- 0.090; K120: 0.795+/-0.096; and significant different with the coefficients in the control group, respectively. This showed that the erythrocyte aggregation process in patients occurred more strong than this in health persons.
Myocardial infarction ; Erythrocyte Aggregation

33 patients who were definitely diagnosed with acute myocardial infarction (MI) using WHO criteria were involved in this study. Statistic method of STARTA 95 was used to compare erythrocyte aggregation after and before using aspirin. Aggregation of erythrocyte increased in the acute stage of MI. Oral aspirin with dose of 0.1g per day for 3 days reduced significantly the erythrocyte aggregation in MI patients, compared with baseline.
Aspirin ; Erythrocyte Aggregation

This article summarizes the pathogenesis of hypercoagulability in β thalassemia patients, including platelet activation, alteration of red blood cell membranes, abnormal expression of adhesion molecules on vascular endothelial cells and iron overload. Clinical evidence, clinical manifestations of hypercoagulable state and thrombosis in β thalassemia and the effect of splenectomy on hypercoagulable state were reviewed. Strategies to prevent and treat the thromboembolic events in β-thalassemia intermedia are also discussed, including transfusion therapy to raise hemoglobin levels, avoidance or delay of splenectomy and a number of treatments in the exploration.
Erythrocyte Aggregation ; Humans ; Thrombophilia ; etiology ; prevention & control ; beta-Thalassemia ; complications

OBJECTIVETo observe the changes of blood viscosity and erythrocyte rheology in mice after acute hypoxic hypoxia (AHH).

METHODSThirty-two Kui-ming mice were randomly divided into control group, AHH group (duplicating AHH model, and divided into 5 min, 8 min, 11 min subgroups), the blood sample was taken out from heart after neck dislocation at corresponding times, for detecting the blood viscosity and erythrocyte rheology indices.

RESULTSCompared with control group, the whole blood viscosity at different shears, whole blood reduced viscosity, whole blood relative viscosity were lower and the erythrocytes aggregation index was higher in AHH 5 min group; the electrophoresis time was longer and the electrophoresis length, migration of erythrocyte were lower in AHH 8 min and AHH 11 min groups. The whole blood reduced viscosity, whole blood relative viscosity, erythrocytes aggregation index in AHH 8 min group were higher, and the erythrocyte deformability index was lower significantly than that of AHH 5 min group, respectively.

CONCLUSIONThese data suggested that the AHH could induce the blood viscosity and electrophoresis ability.

Animals ; Blood Viscosity ; Erythrocyte Aggregation ; Erythrocyte Deformability ; Erythrocyte Indices ; Erythrocytes ; physiology ; Hypoxia ; blood ; etiology ; Male ; Mice ; Mice, Inbred Strains

OBJECTIVETo observe the effects of mesenteric lymph drainage on erythrocyte rheology and blood viscosity in hemorrhagic shock rats.

METHODSWistar rats were randomly divided into sham-shock group, shock group (establishing hemorrhagic shock model), drainage group (establishing hemorrhagic shock model plus drainaging shock mesenteric lymph from hypotension 1 h). At 3 h of hypotension or corresponding time, blood samples were harvested from the abdominal aorta for determining the erythrocytic parameters, erythrocyte electrophoresis, erythrocyte sedimentation rate (ESR) and blood viscosity, and the erythrocytes aggregation index and erythrocyte deformability index were calculated.

RESULTSCompared with the sham-shock group, the red cell contents, hematocrit (HCT), hemoglobin (Hb), mean corpuscular hemoglobin concentration (MCHC), erythrocyte electrophoretic rate and mobility, erythrocyte deformability index, whole blood viscosity, whole blood relative or reduced viscosity at low and high shear rates in shock group were observably lower, and mean corpuscular volume, electrophoretic time of erythrocyte, ESR, K value of equation and K value of emendation, erythrocytes aggregation index, plasma viscosity in shock group were increased markedly; the MCHC, erythrocyte electrophoretic rate and mobility, whole blood viscosity, whole blood relative viscosity at low and high shear rates in drainage group were reduced, and the red blood cell volume distribution width -SD (RDW-SD) was increased remarkably. At the same time, in drainage group, the HCT, RDW-SD, erythrocyte deformability index, whole blood viscosity and relative viscosity at low and high shear rates were higher, the ESR, K value of equation and K value of emendation, erythrocytes aggregation index, plasma viscosity were lower than that of shock group.

CONCLUSIONThe results indicate that the mesenteric lymph drainage could improve the erythrocyte rheological behavior, as a result, improve the hemorrheological properties in hemorrhagic shock rats.

Animals ; Blood Viscosity ; Drainage ; methods ; Erythrocyte Aggregation ; Erythrocyte Deformability ; Lymph ; Male ; Mesentery ; Rats ; Rats, Wistar ; Rheology ; Shock, Hemorrhagic ; blood ; therapy

In order to find out the relationship between blood viscosity and the development of an ischemic symptom(clinical vasospasm) following an aneurysmal subarachnoid hemorrhage(SAH), was checked daily the hematocrit(Hct), blood viscosity and plasma viscosity from admission day to 14 days after SAH in 33 patients. Twelve patients with diseases except those with a SAH were selected as a control group. We analyzed the difference of the average hematocrit, blood viscosity, and plasma viscosity between the control group, non-spasm group and spasm group on admission, and then the period of preoperative, postoperative with mannitolization, and postoperative without mannitolization. We also analyzed the change of those according to the SAH day, and the relationship between the blood viscosity and the level of hematocrit in each group. As a result, aneurysmal SAH patients with clinical vasospasm showed a higher plasma viscosity than the control(1.82+/-0.21 vs. 1.55+/-0.14, respectively : p<0.01) and clinical non-spasm(1.82+/-0.21 vs. 1.66+/-0.12, respectively : p<0.05) group of patients on admission statistically. In the spasm group, the blood viscosity was raised during the spasm risk period(SAH 7-10 days). The level of the hematocrit in the spasm group was lower than the non-spasm group and most of them checked below 30% after surgery. In the relationship between the level of hematocrit and the blood viscosity, the spasm group showed a relatively higher blood viscosity than the non-spasm group. These results suggest that the blood viscosity has some role in the development of ischemic symptoms after a subarachnoid hemorrhage, but major determinants such as erythrocyte aggregation, plasma fibrinogen concentration and platelet aggregation which affect the microcirculation have a more important role. Therefore, when hemodilution are used for prevention or improvement of ischemic symptoms after subarachnoid hemorrhage, the level of the hematocrit and the focus on decreasing the major determinants of the viscosity in microcirculation should be considered.
Aneurysm* ; Blood Viscosity* ; Erythrocyte Aggregation ; Fibrinogen ; Hematocrit* ; Hemodilution ; Humans ; Mannitol ; Microcirculation ; Plasma* ; Platelet Aggregation ; Spasm ; Subarachnoid Hemorrhage* ; Viscosity*

OBJECTIVETo construct and identify the Porphyromonas gingivalis(Pg)ATCC33277 hemagglutinin-2(HA-2)-deficient mutant.

METHODSThe genomic DNA of Pg was isolated from PgATCC33277. The up/down stream genes of HA-2-HA(u), HA(l) were amplified by PCR, and inserted into pSY118 separately which contains a 2.1 kb antibiotic resistance ermF-ermAM cassette. The resultant recombinant plasmid-pSY118-HA was linearized as the gene targating fragment HA-ermF-ermAM and used in the electroporation of PgATCC33277. The Pg HA-2-deficient mutant was screened by allelic exchange. The test of aggregation of red blood cells was used to investigate the function change between PgHA2-deficient mutant and the wild type of PgATCC33277.

RESULTSThe PgHA-2-deficient mutant was identified by PCR. The ability of Pg HA-2-deficient mutant to aggregate red blood cell was significantly decreased compared with the wild type.

CONCLUSIONSHA-2-deficient mutant of Pg ATCC33277 was constructed successfully, which lays a foundation for further study of its biological function.

Alleles ; Erythrocyte Aggregation ; Hemagglutinins ; genetics ; Polymerase Chain Reaction ; Porphyromonas gingivalis ; genetics

OBJECTIVETo observe blood flow features in elderly hypertension patients of different Chinese medical syndrome types and to test their correlation with rheological properties of red blood cells (RBCs) by applying micro-channel array flow analyzer (MC-FAN).

METHODSRecruited were 109 elderly hypertension patients were assigned to phlegm-stasis blocking collateral group (35 cases), yin deficiency yang hyperactivity group (42 cases), Shen deficiency group (32 cases) according to Chinese medical syndrome typing. Besides, another 21 elderly healthy subjects were recruited as the control group. The hemorheology visualization testing of elderly hypertension patients was detected using MC-FAN. The erythrocyte deformation index (DI), erythrocyte aggregation index (EAI), and erythrocyte related plasma ATPase activity were observed. The correlation between the transiting time (TT) of blood hemorheology visualization and rheological indicators of RBCs were analyzed.

RESULTSThe hemorheology visualization TT was significantly prolonged more in the phlegm-stasis blocking collateral group than in the yin deficiency yang hyperactivity group, the Shen deficiency group, and the control group (P < 0.05). Compared with the control group, the DI was significantly lower in the phlegm-stasis blocking collateral group than in the yin deficiency yang hyperactivity group, the Shen deficiency group, and the control group (P < 0.05) when the shear rate was 100 s(-1). Compared with the control group, the plasma Na+ -K(+) -ATPase activity and the plasma Ca2+ -Mg2+ -ATPase activity were significantly lower in elderly hypertension patients (P < 0.01). Among the three groups, plasma Ca2+ -Mg2+ -ATPase activity was significantly lower in the phlegm stasis blocking collateral group than in the Shen deficiency group (P < 0. 05). The hemorheology visualization TT (10, 30, 60, and 100 microL) was negatively correlated with DI. The hemorheology visualization TT at 100 microL was positively correlated with EAI.

CONCLUSIONChanges of hemorheology visualization TT of elderly hypertension patients were significant and correlated with traditional EAI.

Aged ; Case-Control Studies ; Erythrocyte Aggregation ; Erythrocyte Deformability ; Female ; Hemorheology ; Humans ; Hypertension ; blood ; diagnosis ; Male ; Medicine, Chinese Traditional ; methods ; Middle Aged

AIMTo establish reference range of blood theology for different gender by sitting and lying in healthy populations.

METHODSIn 31 volunteers blood were drawed in the postures of sitting and lying to measure blood rheology. Data (between July 2002 and July 2006) of 32854 samples (23436 check-up, 6553 out-patient,2865 in-patient) were collected. Differences between different postures and different gender were compared by Independ-Sample t-test with SPSS 13.0 software and each reference range of blood rheology was established.

RESULTSIn sitting and lying postures 12 parameters of blood rheology showed great significance (P < 0.01). 8 parameters such as whole blood viscosity, blood reduce viscosity, plasma viscosity, hematocrit, were decreased by 9.33% on average while posture was changed from sitting to lying. But index of erythrocyte deformability, erythrocyte electrophoresis presented inverse correlation and were increased by 6.49% on average with the same posture change.Various parameters of blood theology in different gender showed great significance (P < 0.01).

CONCLUSIONThere is significant difference in various parameters of blood rheology in the posture change from sitting to lying. Reference ranges of blood theology of different postures are established which may be used to decrease the rate of misdiagnosis by 8-10 percent.

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Blood Viscosity ; Child ; China ; Erythrocyte Aggregation ; Erythrocyte Deformability ; Female ; Hemorheology ; Humans ; Male ; Middle Aged ; Posture ; physiology ; Reference Values ; Young Adult

In order to provide experimental data for the development and application of drug in clinic, we determined the effects of extracts from different parts of folium perillae (L. ) Britt. on hemorheological parameters, extracted from leaves (folium perillae), seeds (fructus perillae) and peduncles (caulis perillae). The results showed that all extracts from different parts of folium perillae (L. ) Britt. can significantly reduce the whole blood viscosity at low shear rate (10 s(-1)), erythrocyte aggregation index, erythrocyte electrophoresis index (P<0.05), and the whole blood reductive viscosity at low shear rate (10 s(-1)) (P<0.01). Extracts from folium perillae and caulis perillae can significantly decrease erythrocyte deformation index (P<0.05), whereas extracts from fructus perillae can not. Extracts from fructus perillae and caulis perillae can significantly decrease plasma viscosity at low shear rate(10 s(-1)), but extracts of folium perillae can not. Aspirin can only decrease the whole blood reductive viscosity at low shear rate and plasma viscosity (P<0.05). All extracts from different parts of folium perillae (L. ) Britt. had no significant effects on hematocrit, erythrocyte rigidity index, fibrinogen concentration , the whole blood viscosity and the whole blood reductive viscosity at middle and high shear rate (60 s(-1),120 s(-1)).
Animals ; Blood Viscosity ; drug effects ; Erythrocyte Aggregation ; drug effects ; Erythrocyte Deformability ; drug effects ; Male ; Perilla frutescens ; anatomy & histology ; chemistry ; Plant Extracts ; pharmacology ; Plant Leaves ; chemistry ; Plant Stems ; chemistry ; Random Allocation ; Rats ; Rats, Wistar ; Seeds ; chemistry