Objective To explore the correlation between mutations in the promoter region of TBX1 gene and conotruncal heart defects. Methods A total of 621 children with conotruncal heart defects were recruited. Multiplex ligation-dependent probe ampliifcation (MLPA) was used to detect the copy numbers of chromosomal region 22 q 11 . 2 . Children with 22 q 11 . 2 deletion were excluded. Polymerase chain reaction ampliifcation (PCR) and gene sequencing were applied to analyze promoter region of TBX 1 (-2000 ..+1 ) in 605 children with conotruncal heart defects without 22 q 11 . 2 deletion and 588 healthy children. Bioinformatics software was used to predict and analyze the function of the variable loci. Results There were mutations in the promoter region of TBX 1 gene in children with conotruncal heart defects, including 3 single nucleotide polymorphisms (SNP) sites and 7 rare loci. The incidence of mutation was 1 . 7%. The analysis of 7 rare loci by AliBaba 2 . 1 to showed that 3 of them may inlfuence the combination of trans-acting factors and cis-acting elements of the promoter of TBX 1 gene. Conclusion The mutation in the TBX 1 promoter region may be related to the occurrence of conotruncal heart defects.

Objective To understand the situation of nursing teamwork against a background of high-quality nursing service and to explore the influencing factors of nursing teamwork so as to find out practical solutions to improve the level of nursing teamwork.Methods A total of 380 nurses of a classⅢ grade A general hospital in Shanghai Municipality were investigated with the nursing teamwork survey (NTS) by cluster sampling in June 2016. The data were analyzed with SPSS 20.0.Results The median of the total score of NTS among nurses was 4.31 (the full marks are 5). The scores of four dimensions from the highest to the lowest were: team leader (4.75), shared mental models (4.50), trust and support (4.38), team-oriented (4.00). The influencing factors of nursing teamwork included departments, weekly working hours, overtime hours and most often shift nearly three months, whether planed to leave current operating post and departments human resource allocation with significant differences (P<0.05).Conclusions There is still room for improvement in nursing teamwork. Promot teamwork among nurses through reasonably allocating nursing human resources, scientifically scheduling, strengthening humanistic concern and reducing negative effects.

Objective To introduce and translate the English version of Team Development Measure (TDM) into Chinese one and evaluate the reliability and validity of the Chinese version for general practitioner teams to provide a basis for studying the development of the general practitioner teams.Methods According to the Guidelines for the Process of Cross-Cultural Adaptation of Self-report Measures, we completed the translation and culture debugging. Using the convenient sampling method, a total of 26 community health centers were selected which included 85 general practitioner teams for investigation and analyzing the reliability and validity of TDM. The data were statistically analyzed by SPSS 20.0 and AMOS 22.0.Results The correlation (r) between each item and total score of item analysis was 0.251-0.695 (all P<0.001). AllCR values of these items were greater than 3.0 except item 17 and item 23. By use the CFA method,KMO=0.912, and got 4 common factors finally, which could explain 54.3% of overall variance; and had a good fitness CFA confirmed. The Cronbach's α coefficients of the total scale and each dimensions were 0.925,and 0.680-0.916 respectively. The retest reliability was 0.787 (P<0.001).Conclusions The Chinese version of TDM has good reliability and validity and can be used as a tool for measuring the general practitioner team development.