1.Cell affinity of cartilage tissue engineering scaffolds prepared by poly(hydroxybutyrate-co-hydroxyoctanoate)/collagen composite materials
Xing LU ; Yonghong ZHANG ; Erfeng LI ; Zengrong WANG ; Liangqi ZHAO
Chinese Journal of Tissue Engineering Research 2014;(12):1895-1900
BACKGROUND:Many experiments have demonstrated that tissue engineering scaffolds prepared by polymer materials alone or biomaterials cannot meet the requirement of tissue engineering research.
OBJECTIVE:To evaluate biological characteristics and cel affinity of poly(hydroxybutyrate-co-hydroxyoctanoate)/col agen composite scaffold.
METHODS:Tissue engineering scaffolds were prepared by combination of poly(hydroxybutyrate-co-hydroxyoctanoate) and col agen at different proportions (2%, 4%, 6%, 8%and 10%) using solvent casting/particulate leaching method. Inner structure and apertures were observed by scanning electron microscope, and the porosity was determined by liquid displacement method. Rabbit chondrocytes were co-cultured with poly(hydroxybutyrate-co-hydroxyoctanoate)/col agen composite scaffold and poly(hydroxybutyrate-co-hydroxyoctanoate) scaffold. Growth curve of cel s was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and cel adhesion on the scaffolds was observed by scanning electron microscope.
RESULTS AND CONCLUSION:The pore size and porosity of the composite scaffold were about 200μm and (85±2)%, respectively. The cel affinity dynamical y increased with the increasing of proportion of col agen. Compared with the poly(hydroxybutyrate-co-hydroxyoctanoate) scaffold, the poly(hydroxybutyrate-co-hydroxyoctanoate)/col agen composite scaffolds are better to improve cel adhesion and proliferation, with favorable cel ular affinity.
2.The Gender and Clinical Symptoms Difference in Depression of Parkinson’s Disease
Yamin WANG ; Erfeng LI ; Li JIANG
Journal of Apoplexy and Nervous Diseases 2020;37(11):989-993
Objective We aimed to analyze the gender differences by evaluating the motor and non-motor symptoms among the patients with parkinson’s disease and the patients with depression of parkinson’s disease,and to explore the possible pathogenesis further and make sex-specific therapeutic strategies. Methods Two hundred patients with parkinson’s disease from both the outpatients department and the ward were included in this analysis. There were 56 patients whose HAMD scores exceeded 19,which were divided into the depession of parkinson’s disease (dPD) group。There are 144 patients whose HAMD scores under 20,which were divided into the non-depession of parkinson’s disease (ndPD) group. The scores from all the scales were analyzed using statistical software. Results ①The depression incidence of females was significantly higher than that of males in PD patients (P<0.05). ②Rigidity score and Bradykinesia score in males were significantly higher than that in females in dPD patients (P<0.05). Tremor score in males was significantly lower than that in females in dPD patients (P<0.05). ③A significantly higher score in mood/apathy and feel problem/hallucination domains were observed in female PD patients (P<0.05). ④The HAMA score,HAMD score,NMSQ total score of dPD patients were all significantly higher than that of ndPD patients (P<0.05). The PDSS score of dPD patients was significantly lower than that of ndPD patients (P<0.05). The tremor score,rigidity score,postural instability score,bradykinesia score,UPDRS-III score of dPD patients were all significantly higher than that of ndPD patients (P<0.05). ⑤The HAMD scores correlated with levodopa equivalent doses (Led) (r=-0.31,P=0.01),H-Y stage (r=0.23,P=0.03),PDSS (r=0.36,P=0.01) and rigidity (r=0.27,P=0.04) scores. Conclusion ①There are gender differences bewteen males and females in motor disorders in depression of parkinson’s disease. ②The motor disorders and non-motor disorders were more severe in depression of parkinson’s disease than in non-depression of parkinson’s disease. ③The HAMD score in depression of parkinson’s disease correlated with levodopa equivalent doses,the motor disorders and the non-motor disorders. ④The spectrum of motor and non-motor symptoms showed different gender distribution in PD and dPD patients. Gender-specific therapeutic agents hold particula attention for developing treatments with optimal efficaty in men and women in the future.
3.Application of miniprobe ultrasonic endoscope in evaluating the depth of invasion in colorectal laterally spreading tumors
China Journal of Endoscopy 2024;30(3):25-30
Objective To analyze the accuracy and influencing factors of miniprobe ultrasonic endoscope in evaluating submucosal infiltration of colorectal laterally spreading tumor(LST).Method A retrospective analysis was conducted on the clinical data of 213 patients(268 lesions in total)with colorectal LST who underwent endoscopic submucosal dissection(ESD)treatment from June 2018 to August 2021.We summarized the clinical pathological characteristics and miniprobe ultrasonic endoscope examination results of LST,then analyzed the accuracy of miniprobe ultrasonic endoscope examination and the risk factors affecting the accuracy of miniprobe ultrasonic endoscope examination.Results The accuracy rate of miniprobe ultrasonic endoscope examination was 93.28%,and there was a statistically significant difference in the accuracy rate of miniprobe ultrasonic endoscope examination between different lesion surface morphologies(P = 0.000).Multivariate Logistic regression analysis showed that mix-ed nodule and false depression lesions were risk factors for inaccurate EUS assessment.Conclusion Colorectal LST is a special type of tumor,and miniprobe ultrasonic endoscope examination has a high accuracy in evaluating its infiltration depth.The surface morphology of the lesion is a risk factor that affects the accuracy of miniprobe ultrasonic endoscope examination.When the lesion is a nodule mixed type or pseudo depressed type,it can easily lead to inaccurate miniprobe ultrasonic endoscope examination.
4.Analysis of risk factors and construction of prediction model of pathological diagnosis upgrading after resection of colorectal laterally spreading tumors
Erfeng LI ; Jing PANG ; Libin ZHANG ; Wenbin ZHANG ; Feng WANG ; Bin GUO
Chinese Journal of Digestion 2024;44(6):391-397
Objective:To investigate the risk factors affecting pathological diagnosis upgrading after resection of colorectal laterally spreading tumor (LST).Methods:From June 2018 to December 2022, the clinical data of 256 patients with LST (297 lesions) admitted to Shanxi Provincial Cancer Hospital were retrospectively included as an modeling group.From January 2023 to January 2024, 125 patients with LST (129 lesions) were collected as an external validation group. The pathological diagnosis of endoscopic forceps biopsy (EFB) samples and the resected LST tissue of modeling group were compared, and the patients were divided into pathological non-difference group and pathological upgrading group. The clinical data such as gender, age, body mass index (BMI), pre-resection carcinoembryonic antigen levels, drinking history, smoking history, family history of colorectal cancer, and whether complicated with underlying diseases as well as endoscopic surface morphological features such as lesion size, morphological features, and lesion location were compared between the two groups. Chi-square test was used for statistical analysis, and multivariate logistic regression analysis was used to identify the risk factors for pathological diagnosis upgrading after resection. Based on the independent risk factors, the prediction models were established and validated by nomogram. The receiver operating characteristic curve (ROC) of repeated samples within the modeling group and external validation growp was plotted, and the area under the curve (AUC) was used to evaluate the predictive value of the model.Results:The proportion of patients with family history of colorectal cancer in the pathological upgrading group was higher than that of the pathological non-difference group (38.7%, 12/31 vs. 22.2%, 50/225), and the difference was statistically significant ( χ2=4.04, P=0.045). There were statistically significant differences in lesion size (63.9% (23/36) and 44.4% (116/261) lesions with long diameter ≥2 cm, respectively), surface morphological characteristics (flat elevated type accounted for 8.3% (3/36) and 22.6% (59/261), granular uniform type accounted for 11.1% (4/36) and 28.0% (73/261), nodular mixed type accounted for 44.4% (6/36) and 24.9% (65/261), pseudo-depressed type accounted for 36.1% (13/36) and 24.5% (64/261)), and lesion location (distal colon accounted for 22.2% (8/36) and 33.3% (87/261), proximal colon accounted for 16.7% (6/36) and 28.7% (75/261), and rectum accounted for 61.1% (22/36) and 37.9% (99/261)) between the pathological upgrading group and the pathological non-difference group ( χ2=4.80, 12.62 and 7.08, all P<0.05). The results of multivariate logistic regression analysis showed that family history of colorectal cancer ( OR=2.211, 95% confidence interval (95% CI) 1.005 to 4.861, P=0.049), lesion length ≥ 2 cm ( OR=2.212, 95% CI 1.074 to 4.555, P=0.031), nodular mixed subtype ( OR=4.841, 95% CI 1.343 to 17.455, P=0.016), pseudo-depressed subtype ( OR=3.995, 95% CI 1.084 to 14.721, P=0.037), and lesion in rectum ( OR=2.417, 95% CI 1.024 to 5.705, P=0.044) were independent risk factors for pathological diagnosis upgrading after LST resection. A nomogram was established based on these four risk factors, with a ROC AUC of 0.833 (95% CI 0.752 to 0.913). The external validation results demonstrated that the ROC AUC was 0.848 (95% CI 0.736 to 0.960), the sensitivity was 0.737, the specificity was 0.972, the maximum Youden index was 0.712, and the overall accuracy was 0.868. Conclusions:Family history of colorectal cancer, lesion length ≥ 2 cm, lesion in rectum, and nodular mixed or pseudo-depressed subtypes may affect the accuracy of pathological diagnosis of LST lesions by EFB, and leading to pathological diagnosis upgrading after resection. The prediction model based on these four factors has good predictive efficacy in pathological diagnosis upgrading after LST resection.
5.Efficacy and safety of endoscopic argon ion coagulation combined with mesalazine enteric fluid in the treatment of chronic radiation proctitis
Libin ZHANG ; Wenbin ZHANG ; Bin GUO ; Erfeng LI
Cancer Research and Clinic 2024;36(5):381-385
Objective:To investigate the efficacy and safety of endoscopic argon ion coagulation combined with mesalazine enteric fluid in the treatment of chronic radiation proctitis with hemorrhage.Methods:A retrospective case control study was conducted. The clinical data of 82 patients with chronic radiation proctitis with hemorrhage admitted to Shanxi Province Cancer Hospital from January 2019 to January 2022 were retrospectively analyzed. According to the treatment methods, all patients were divided into the observation group (endoscopic argon ion coagulation combined with mesalazine enteric fluid retention enema, 44 cases) and the control group (0.9% NaCl solution 20 ml + thrombin 1 000 U combined with mesalazine enteric fluid retention enema, 38 cases). The clinical efficacy, proctoscopy scores, laboratory indexes [C-reactive protein (CRP), hemoglobin (Hb)] and the adverse reactions of the two groups 1 month after treatment were compared.Results:There were no statistically significant differences in gender, age, body mass index and disease classification between the two groups (all P > 0.05). After 1 month of treatment, the total effective rate of the observation group was higher than that of the control group [93.18% (41/44) vs.76.32% (29/38)], and the difference was statistically significant ( χ2 = 4.64, P = 0.031). The degree of intestinal injury, anal pain and naked blood stool in the observation group were lower than those in the control group (all P < 0.05). The level of CRP 1 month after treatment was lower than that before treatment in both groups, and the level of Hb 1 month after treatment was higher than that before treatment in both groups (all P < 0.001); and there were no statistically significant differences in the levels of CRP ahd Hb before treatment and 1 month after treatment between the both groups (all P > 0.05). There was no statistically significant difference in the adverse reactions between the two groups ( P > 0.05). Conclusions:Endoscopic argon ion coagulation combined with mesalazine enteric fluid in the treatment of chronic radiation proctitis with hemorrhage has a favorable effect and a good safety, which can improve the symptoms of hematochezia, diarrhea, anemia and improve the quality of life of patients.
6.A case -control study of the effects of surgical history on chemotherapy -induced nausea and vomiting
Bo SUN ; Erfeng ZHANG ; Lu CHEN ; Xun LIU ; Shufang LI ; Huanqing MA ; Lili PAN ; Danna LIU ; Huipin WANG
China Pharmacy 2022;33(19):2378-2383
OBJECTIVE To explore the influence of surgical history on chemotherapy -induced nausea and vomiting (CINV). METHODS A retrospective case -control study was adopted ,with 824 patients undergoing chemotherapy as the object . A total of 27 items were collected ,including demographic data ,medical history data ,pre-chemotherapy data ,and chemotherapy treatment status. Logistic regression model was used to analyze the relationship between the history of surgery and the risk of CINV . The multiple models were constructed to correct potential confounding factors ,and subgroup analysis was performed on patients with surgical history . RESULTS The incidence of CINV was higher in patients with surgical history . The statistical result before adjustment was [OR=1.72,95%CI(1.31,2.28),P<0.001];after adjusting potential confounding factors ,the statistical result was [OR=1.78,95% CI(1.28,2.48),P=0.001]. In the subgroup analysis ,the time between surgery and chemotherapy was different , and the impact of surgical history on CINV was different ,and the results were statistically significant (P=0.027). The risk of CINV showed decreasing trend with the time ,and the results were statistically significant (P for trend ≤0.050). Compared with patients who had not undergone surgery ,patients who had undergone surgery within one year had a higher risk of CINV [OR= 2.33,95%CI(1.52,3.59),P<0.001]. CONCLUSIONS Patients with surgical history are more prone to CINV ,and the risk of CINV shows a downward trend in the length of time from surgery .