1.Isolation and identification of Roseomonas strain from the ticks
Erchen QIU ; Fang ZHANG ; Wei LIU ; Xiaoming WU ; Hong YANG ; Wuchun CAO
Chinese Journal of Microbiology and Immunology 2009;29(1):29-32
objective To identify the three strains of pink-pigmented bacteria isolated from ticks.Methads 16S rDNA sequence analysis,16S rDNA similarity searching,bacterial morphological,physiological and biochemical tests and fatty acids analysis were used.Results The bacterial strains were pinkpigment,gram negative,strictly aerobic coccobacillus.They grow optimally at 37℃,while not in the presence of≥5% NaCl.The tests showed positive result for urease,D-glucose,citrate,UV absorption,and catalase.The predominant fatty acids were C16:0,C17:0,C18:1 ωgc and C19:0 cyc.Comparative 16S rDNA sequences analysis revealed that the strains were closely related to Roseomonas cervicalis and could reach up to 99.1% identity.Conclusion Based on phenotypic,biochemical and phylogenetic data,we propose these three isolates represent distinct species in genus Roseomonas,of which ticks may serve as a potential vector.
2.Effects of job burnout and work satisfaction on the quality of life about army recruits
Jianjiang ZHANG ; Jimin JIA ; Ning TAO ; Hua TIAN ; Erchen QIU ; Zhixin SONG ; Jiwen LIU
Chinese Journal of Behavioral Medicine and Brain Science 2017;26(1):64-69
Objective To investigate the quality of life of army recruits in their basic military training period,and to analyze the influence of job burnout and work satisfaction on their quality of life.Methods A total of 625 recruits enrolled in 2014 of Xinjiang Army troop were chosen as subjects in this study by stratified cluster random sampling.Quality of life was assessed by Chinese version SF-36(Short-Form Health Survey Scale),job burnout was assessed by Chinese version Maslach Burnout inventory general survey (MBI-GS) and work satisfaction was assessed by Chinese version Minnesota Satisfaction Questionnaire (MSQ).Results The scores of PCS and MCS about 597 recruits were (85.93±12.62) and (81.10±14.12) respectively.According to demographic characteristics,the score of physiological function was lower than that of non-smoking group (t=2.009,P<0.05),the score of role physical was lower than that of non-smoking group (t=2.617,P<0.05),and the score of PCS was lower than that of non-smoking group (t=2.141,P<0.05).As to except reported health transition,there were negative correlations between emotional exhaustion,depersonalization and other SF-36 scales (r=-0.344~-0.661,P<0.01) respectively.There were negative correlations between personal accomplishment and vitality (r=-0.204,P<0.05),role emotional (r=-0.239,P<0.05),mental health (r=-0.289,P<0.05) and MCS (r=-0.276,P<0.05) respectively.Work satisfaction and quality of life was positively correlated (r=0.243~0.635,P<0.01).As to the independent variable,regression analysis showed that emotional exhaustion (β=-4.732,P<0.01),Minnesota external satisfaction (β=0.783,P<0.01) influenced PCS,the difference was statistically significant.Emotional exhaustion(β=-6.534,P<0.01),pre-enlistment place of residence (β=-5.319,P< 0.05),Minnesota external satisfaction (β=0.813,P<0.01)influenced MCS,the difference was statistically significant.Conclusion Job burnout and work satisfaction influence the quality of life about the army recruits,the more job burnout signify the lower quality of life,and its influence on mental health is more significant.The higher work satisfaction signify the higher quality of life.
3.Study on co-infection of tick-borne pathogens in Ixodes persulcatus in Charles Hilary, Xinjiang Uygur autonomous region
Xiaoming LIU ; Guilin ZHANG ; Ran LIU ; Xiang SUN ; Zhong ZHENG ; Erchen QIU ; Xiaoling MA
Chinese Journal of Epidemiology 2015;36(10):1153-1157
Objective To study the co-infection of tick-borne pathogens in Ixodes persulcatus collected in Charles Hilary,Xinjiang Uygur autonomous region (Xinjiang).Methods Ticks were collected by flagging in grassy areas in Charles Hilary,Xinjiang from June 2012 to June 2013.The 5S-23S rRNA intergenic region from Borrelia (B.) burgdorferi,16S rRNA gene from Anaplasma,ompA gene from spotted fever group Rickettsia,comI gene from Coxiella (C.) burneti and Nss-rRNA gene from Babesia were amplified with nested polymerase chain reaction (nested PCR),respectively.Results Among 204 lxodes persulcatus,104 were positive for tick-borne pathogens with the positive rate of 50.98%,and among them the positive rates of B.burgdorferi,spotted fever group Rickettsia and Anaplasma phagocytophilum were 34.31% (n =70),28.92% (n =59),9.31% (n =19),respectively.And no C.burnetii and Babesia were detected.The overall co-infection rate was 19.12% (39/204),the co-infection rate was 16.18%(33/204) for B.garinii and spotted fever group Rickettsia,4.90% (10/204) for B.burgdorferi and Anaplasma phagocytophilum,2.94%(6/204) for spotted fever group Rickettsia and Anaplasma phagocytophilum and 2.45% (5/204) for B.burgdorferi,Anaplasma phagocytophilum and spotted fever group Rickettsia.Conclusion The results indicated that the natural co-infections of B.garinii,B.afzelii,Anaplasma phagocytophilum and spotted fever group Rickettsia existed in Charles Hilary Ixodes persulcatus collected in Xinjiang.
4. Mediator effect analysis of the trait coping style on job stress and fatigue of the military personnel stationed in plateau and high cold region
Jianjiang ZHANG ; Jimin JIA ; Ning TAO ; Zhixin SONG ; Hua GE ; Yu JIANG ; Hua TIAN ; Erchen QIU ; Jinhua TANG ; Jiwen LIU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2017;35(3):176-180
Objective:
To investigate the fatigue status of military personnel stationed in plateau and high cold region, and to analyze the mediator effect of trait coping style on job stress and fatigue.
Methods:
In October 2010, with the method of cluster random sampling survey, 531 military personnel stationed in plateau and high cold region were chosen as subject. The fatigue status were evaluated by the Chinese version multidimensional fatigue inventory (MFI-20) , job stress were evaluated by the Job Stress Survey (JSS) , and trait coping style were evaluated by the Trait Coping Style Questionnaire (TCSQ) .
Results:
According to the information of different population characteristics, mean rank of physical fatigue about the urban (town) group were higher than that of rural group (