1.Evaluation of enzymum system@(ES-300) for enzyme linked immunosorbent assay: comparison with RIA and CLIA for T3, T4, fT4 and TSH.
Kyung Soon SONG ; Young Kyu SUN ; Hae Ryun KIM ; Woon Young RYU ; Baek Soo KIM
Korean Journal of Clinical Pathology 1993;13(1):7-11
No abstract available.
Enzyme-Linked Immunosorbent Assay*
2.Evaluation of enzymum system@(ES-300) for enzyme linked immunosorbent assay: comparison with RIA and CLIA for T3, T4, fT4 and TSH.
Kyung Soon SONG ; Young Kyu SUN ; Hae Ryun KIM ; Woon Young RYU ; Baek Soo KIM
Korean Journal of Clinical Pathology 1993;13(1):7-11
No abstract available.
Enzyme-Linked Immunosorbent Assay*
3.Evaluation of enzymum system@(ES-300) for enzyme linked immunosorbent assay: comparison with RIA and CLIA for T3, T4, fT4 and TSH.
Kyung Soon SONG ; Young Kyu SUN ; Hae Ryun KIM ; Woon Young RYU ; Baek Soo KIM
Korean Journal of Clinical Pathology 1992;12(1):7-11
No abstract available.
Enzyme-Linked Immunosorbent Assay*
4.Detection of anti-borrelia burgdorferi antibody by enzyme-linked immunosorbent assay in Korea.
Myoung Sook KOO ; Eui Chong KIM ; Woon Sup HAN
Korean Journal of Clinical Pathology 1991;11(2):435-443
No abstract available.
Enzyme-Linked Immunosorbent Assay*
;
Korea*
5.A Case of ELISA negative AIDS Who was diagnosed by Polymerase diagnosed by Chain Reaction: An Autopsy Case.
Jun Won CHUNG ; Jiso RYU ; Jin Won CHUNG ; Soo Jin KANG ; I Nae PARK ; Young Keol CHO ; Shin Kwang KHANG ; Jun Hee WOO ; Yang Soo KIM
Korean Journal of Infectious Diseases 2001;33(3):227-230
No abstract available.
Autopsy*
;
Enzyme-Linked Immunosorbent Assay*
6.Expression and use of Truncated Recombimnant Flagellin Protein ( FlaB ) in ELISA for Diagnosis of Leptospirosis.
Cheon Kwon YOO ; Myo Ah PAIK ; Hae Kyung LEE ; Man Suck PARK ; Mi Yeoun PARK
Journal of the Korean Society for Microbiology 2000;35(5):365-365
No Abstract Available.
Diagnosis*
;
Enzyme-Linked Immunosorbent Assay*
;
Flagellin*
;
Leptospirosis*
7.Establishment of Measurement of Human Cytomegalovirus with in situ ELISA.
Eung Soo HWANG ; Jin Hee KIM ; Chung Kyu PARK ; Chang Yong CHA
Journal of the Korean Society of Virology 2000;30(2):125-130
No Abstract Available.
Cytomegalovirus*
;
Enzyme-Linked Immunosorbent Assay*
;
Humans*
8.Development of Enzyme Linked Immunosorbent Assay for Erythropoietin.
Ki Hong KIM ; Jung Hyun SHIM ; Min Chul CHO ; Jeong Woo KANG ; Hyo Eun YOON ; Do Young YOON ; Jong Wan KIM ; Dong Ju SON ; Jae Woong LEE ; Eun Sook JEONG ; Jin Tae HONG ; Dong Cheul MOON
The Korean Journal of Laboratory Medicine 2006;26(3):185-191
BACKGROUND: The aim of our study was to optimize and establish erythropoietin (EPO) enzyme linked immunosorbent assay (ELISA) system. METHODS: We prepared several monoclonal and polyclonal antibodies specific to human-EPO. The best combinations of antibodies for coating and detecting antibodies were selected for the establishment of ELISA. We tested several methods such as a competitive EIA and a sandwich ELISA. RESULTS: The best sandwich ELISA was optimized compared to competitive EIA when purified polyclonal antibody (PoAb) was used as a coating antibody and biotinylated PoAb as a detecting antibody. This sandwich ELISA easily detected EPO when PoAb pairs were used compared to the ELISA using monoclonal antibody and PoAb. There were no significant differences between the effects of various blocking solutions on the performance of sandwich ELISA using biotinylated antibody. The ELISA system using PBST containing 3% BSA as a blocking solution can sensitively detect EPO (10 mU/mL) in a broad range of EPO concentrations (10-2,000 mU/mL) and there were cross-reactions with other cytokines). CONCLUSIONS: EPO can be easily determined by using biotinylated PoAb as a detecting antibody and another PoAb as a coating antibody.
Antibodies
;
Enzyme-Linked Immunosorbent Assay*
;
Erythropoietin*
9.Study on immune response of antibodies IgA1 and IgG fron sera of hepatoma and acute leukaemia patients by lectin-ELISA and lectin-protein A
Journal of Medical and Pharmaceutical Information 2003;9():23-25
Healthy human serum IgA, purified lectin-sepharose 4B column affinity chromatography was assessed by sodium dodecyl sulfate polyacrylamide electrophresis. The lectin from wild Jackfruit reacted specifically to IgA1 from human serum. This lectin used as antigen in the technique of ELISA-Lectin to determine the concentration of IgA1 from healthy and pathological human sera. Using protein A from Staphylococcus aureus isolated from Vietnamese infectious patients to capture IgG average concentration of IgA1 from healthy human serum as 14.37 mg/ml. No change of IgG concentration from patients sera (hepatoma and leukemia) was obtained in comparison with healthy subjects
Carcinoma, Hepatocellular
;
Enzyme-Linked Immunosorbent Assay
;
Leukemia
;
10.Quick diagnosis of japanese encephalitis by MAC-ELISA technique
Journal of Practical Medicine 2004;481(6):5-6
On 138 serum samples taken in the first 7 days of acute syndrome of brain, by MAC-ELISA technique 95 samples were determined as positive, 68.84% was positive. 43 samples taken in the first 7 days of the disease there was no detected antivirus IgM antibody but this could not concluded as negative, but may be a late antibody response or may be another cause of acute brain syndrome. For assuring the accuracy of the diagnosis, the samples taken in the first 7 days of the disease must be taken again after 7 day to verify the results.
Encephalitis, Japanese
;
Diagnosis
;
Enzyme-Linked Immunosorbent Assay
Result Analysis
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