2.Enterovirus Infection in Adults Presenting with Nonspecific Febrile Illness during Summer.
Areum DUREY ; Young Su JE ; Hea Yoon KWON ; Jae Hyoung IM ; Ji Hyeon BAEK ; Sun Myoung LEE ; Young Kyoung PARK ; Jin Soo LEE
Infection and Chemotherapy 2017;49(2):140-141
No abstract available.
Adult*
;
Enterovirus Infections*
;
Enterovirus*
;
Humans
3.Detection of Enteroviruses and Mammalian Reoviruses by RT-PCR and Integrated Cell Culture-PCR in CPE-positive Surface Water Samples.
Hee Jung KIM ; Hyun Ae KO ; Sang Hyun KIM
Journal of Bacteriology and Virology 2004;34(4):331-338
The environmental water samples assayed by total culturable virus assay (TCVA) were evaluated by reverse transcription-polymerase chain reaction (RT-PCR) and integrated cell culture-PCR (ICC-PCR) method for enteroviruses and reovirus since they are the usually detected virus groups by culture assays. The detection sensitivities of the TCVA, RT-PCR, and ICC-PCR were compared and the overall reliability of the detection was analyzed to confirm environmental samples for enteric viruses. A total of eight samples from different areas was analyzed by performing TCVA, RT-PCR, and ICC-PCR. Virus concentrations in surface water samples ranged from 1.03 to 47.3 most probable numbers of infectious units (MPN) per 100 liters. When primers specific for both enteroviruses and reoviruses were used in both RT-PCR and ICC-PCR, all the samples (100%) were positive for the viruses. Reoviruses were the most frequently detected ones among the samples. According to the sequence results of enteroviruses, five of the samples were contaminated by coxsackievirus type B3, and the rest by coxsackievirus type B6, echovirus type 30, or vaccine strain poliovirus type 3. It was observed that both enteroviruses and reoviruses were detected concurrently in all CPE-positive environmental water samples.
Enterovirus B, Human
;
Enterovirus*
;
Poliovirus
;
Water*
4.Detection of Enteroviruses and Mammalian Reoviruses by RT-PCR and Integrated Cell Culture-PCR in CPE-positive Surface Water Samples.
Hee Jung KIM ; Hyun Ae KO ; Sang Hyun KIM
Journal of Bacteriology and Virology 2004;34(4):331-338
The environmental water samples assayed by total culturable virus assay (TCVA) were evaluated by reverse transcription-polymerase chain reaction (RT-PCR) and integrated cell culture-PCR (ICC-PCR) method for enteroviruses and reovirus since they are the usually detected virus groups by culture assays. The detection sensitivities of the TCVA, RT-PCR, and ICC-PCR were compared and the overall reliability of the detection was analyzed to confirm environmental samples for enteric viruses. A total of eight samples from different areas was analyzed by performing TCVA, RT-PCR, and ICC-PCR. Virus concentrations in surface water samples ranged from 1.03 to 47.3 most probable numbers of infectious units (MPN) per 100 liters. When primers specific for both enteroviruses and reoviruses were used in both RT-PCR and ICC-PCR, all the samples (100%) were positive for the viruses. Reoviruses were the most frequently detected ones among the samples. According to the sequence results of enteroviruses, five of the samples were contaminated by coxsackievirus type B3, and the rest by coxsackievirus type B6, echovirus type 30, or vaccine strain poliovirus type 3. It was observed that both enteroviruses and reoviruses were detected concurrently in all CPE-positive environmental water samples.
Enterovirus B, Human
;
Enterovirus*
;
Poliovirus
;
Water*
7.Epidemics of aseptic meningitis in Seoul area during 1989-90.
Eun young CHO ; Mi Koo KANG ; Soo Jong HONG ; Ki Soo KIM ; Young Seo PARK ; In Sook PARK ; Hyung Nam MOON ; Chang Yee HONG
Journal of the Korean Pediatric Society 1991;34(11):1565-1572
No abstract available.
Enterovirus
;
Meningitis, Aseptic*
;
Seoul*
8.Sequence Varation of 5(1) Nontranslating Region of Enterovirus Isolated from Patients with Aseptic Meningitis.
Young Chul PARK ; Jung Hyun NAM ; Chul Hyun JOO ; Mi Sun MOON ; Kyoung Won CHUNG ; Heui Ran LEE ; Yung Keol CHO ; Yoo Kyum KIM
Journal of Bacteriology and Virology 2001;31(1):85-94
No abstract available.
Enterovirus*
;
Humans
;
Meningitis, Aseptic*
9.Integrated Cell Culture-PCR Detection of Enteroviruses and Reoviruses in Water Sources in Gyeonggi-do.
Kyung A KIM ; Jong Chan KIM ; Hoan Uck KO ; Jung Bock LEE ; Young Sug KIM ; Yong Bae PARK ; Myung Jin LEE ; Myung Gill KIM ; Jae Kwan KIM ; Eun Mi PARK
Journal of Bacteriology and Virology 2008;38(2):77-87
The integrated cell culture-PCR (ICC-PCR) method has been suggested as an improved method for detection of viruses in water environments. We tested 57 source waters including finished water samples in Gyeonggi-do for enteric viral contamination using total culturable virus assay (TCVA) using BGMK cells and ICC-PCR. Nineteen of the 57 source water samples (33.3%) exhibited the cytopathic effect (CPE) on BGMK cells and no finished water did exhibited CPE. Nineteen samples (33.3%) of the 57 were positive for reoviruses. For the enteroviruses, only 3 samples (5.3%) of the 57 samples showed positive results. By using ICC-PCR method, 202 flasks from source water samples were positive for enteroviruses and reoviruses. Three samples from source water were positive for both viruses. However, any flasks tested was not co-infected with two types of viruses. While the enteric viral frequencies in TCVA and ICC-PCR were similar, the viral frequency for reoviruses at first passage in two type of method was higher in ICC-PCR (94.7%) than TCVA (56.9%).
Enterovirus
;
Viruses
;
Water
10.Isolating enterovirus and poliovirus isolated in patients with acute flaccid paralytic syndrome in 2005
Thanh Thi Hien Nguyen ; Binh Thanh Nguyen
Journal of Preventive Medicine 2007;17(6):11-16
Background: Paralysis has been eliminated in Vietnam in 2000. To maintain the achievement, Vietnam needs to have more than 90% of children under 1 age drinking 3 doses of OPV, controlling cases of acute flaccid paralysis and diagnosing for virology in laboratory to determine causes of the disease. Results of laboratory identifying Polio virus and enterovirus in patients with acute flaccid paralysis is evidence of successful in paralytic elimination annually in Vietnam.\r\n', u'Objectives: To of isolate polio virus and enterovirus in patients with acute flaccid paralysis suspected paralysis in 2005.\r\n', u'Subjects and methods: The study included 267 cases with acute flaccid paralysis from 29 provinces, cities in the North and six provinces in the Central of Vietnam during 2005.\r\n', u'Results:Results of surveillance for acute flaccid paralysis and indicators of samples tested in 2005 have reached the criteria regulated by the World Health Organization. 8 poliovirus strains and 56 other non-polio enterovirus strains were isolated from 538 stool specimens of above patients. All isolated poliovirus strains were confirmed as Sabin-like strains. 56 non-polio enterovirus strains included coxsaxkie A (3), conxsackieB (5), echoviruses (34), enterovirus 71 (1) and untyped enteroviruses (13). Wild polio virus was not isolated in 2005.\r\n', u'Conclusion: The result confirmed successfulness in maintaining of poliomyelitis free status in the North and 6 provinces of the Central of Vietnam in 2005.\r\n', u'\r\n', u'\r\n', u'
Paralysis
;
Enterovirus
;
Poliovirus