Objective To observe the effect of penetrating electro-acupuncture at scalp-acupoints on the motor function and oxidative stress action of mice with Parkinson's disease (PD).Methods Mouse model of PD was established by 5-day intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP,30 mg/kg),and then PD mice were given electro-acupuncture set at different parameters.The effect of the electroacupuncture on the behavior and expression of tyrosine hydroxylase (TH) of mice were observed to screen out the appropriate treatment parameters.Healthy C57BL/6 mice were randomly divided into blank group,model group,positive group (madopar,6.25 mg/kg) and electro-acupuncture group.The mice except for blank group were given intraperitoneal injection of MPTP to induce PD,and then the PD mice of positive group and electroacupuncture group were treated with madopar(6.25 mg/kg) and electro-acupuncture respectively from the 6th day for 7 continuous days.Behavioral test was carried out on the 5~ modeling day and on the Th electro-acupuncture treatment day to evaluate the motor function of the mice.After the mice were killed and their brains were isolated,mitochondrial complex Ⅰ ～Ⅳ activities,reactive oxygen species(ROS) level,malondialdehyde (MDA) content,and total activities of superoxide dismutase (SOD) in the cerebral mitochondria were determined to evaluate the effect of electro-acupuncture at acupoints on anti-oxidative stress of PD mice.Results The motor function of PD mice was improved,the activity of mitochondrial complex Ⅰ was increased,the contents of ROS and MDA in cerebral mitochondria were decreased,and the activity of SOD was increased in the electro-acupuncture group,the differences being significant compared with those of the model group (P ＜ 0.05 or P ＜ 0.01).Conclusion Penetrating electro-acupuncture at scalp-acupoints can increase the anti-oxidative ability of the cerebral mitochondria and improve the motor function of PD mice.

[ ABSTRACT] AIM:To investigate the effect of triptolide on the inhibition of microglial activation in 1-methyl-4-phenyl pyridinium ( MPP+)-induced hemiparkinson disease rats.METHODS:The rat model of Parkinson disease was es-tablished by intranigral injection of MPP +.The rats were randomly divided into sham group, MPP+group, triptolide group and vehicle group.The survival of dopaminergic neurons was detected by the immunofluorescence of tyrosine hydroxylase ( TH) in the substantia nigra ( SN) .The activation of microglia was determined by immunofluorescence of OX-42 ( micro-glia marker) in the SN.The expression of chemokine receptor CX3CR1 in SN was measured by Western blotting.RE-SULTS:Intranigral injection of MPP+increased the fluorescence intensity of the microglial marker, and promoted DA neu-ron degenerative death.Immunohistological analysis showed that the OX-42 density was decreased (P<0.01) and tyrosine hydroxylase (TH) positive neurons were increased in the triptolide group (P<0.01).The expression of CX3CR1 was lower in triptolide group than that in model group (P<0.05).CONCLUSION:Triptolide may improve PA neurons func-tion in MPP+-induced rats through inhibiting CX3CR1 expression and microglial activation.

Objective:To explore the feasibility and cosmetic effect of repairing partial breast defects with pedicled lateral thoracic artery perforator(LTAP) flap.Methods:Pedicled LTAP flap was applied to repair partial breast defects in 12 patients from the Department of Breast Surgery of Shenzhen Maternal and Child Health Hospital of Southern Medical University from May 2019 to October 2020. Before the operation, the Doppler ultrasound was used to explore the perforator branch of the lateral thoracic artery in the 3rd to 4th intercostal area of the lateral thoracic wall, and a transverse or nearly transverse pedicled LTAP flap containing the perforator branch was designed. During the operation, the flap was elevated from the deep surface of the deep fascia, through which the lateral thoracic vessels were faintly visible. Then the vascular direction was determined with an audible Doppler blood flow detector. From the deep fat layer and the deep surface of the deep fascia, the pedicle with a width of about 1.2 cm and a thickness of about 0.5 cm was formed by careful dissection along the main vessel to the axillary direction. The flap was transferred to the breast defect after the epidermis of the flap was removed. The survival of the flaps was observed, and the reconstructed breast shape was evaluated.Results:A total of 12 patients were included. All patients were female, aged from 32 to 58 years old, with an average of 41.7 years. Among them, there were 8 cases of breast cancer and 4 cases of non-lactation mastitis. The lesions were all located in the lateral breast area. In this study, the tissue weight of tumor or inflammatory lesions was (78.3±5.1) g(56-92 g), and the LTAP flap with pedicle was (9.3±0.6) cm (8.2-12.5 cm)in length and (5.6±0.4) cm(3.8-7.2 cm) in width. In this group, 1 patient developed an infection of the donor site drainage entrance. After wound dressing and taking oral antibiotics, the wound healed quickly and healed well. Postoperative follow-up was conducted for 2-17 months, with an average of 8.2 months. There were 10 cases with good cosmetic effect and 2 cases with average cosmetic effect, and there were no patients with poor cosmetic effect. All patients were satisfied with the shapes of the reconstructed breasts. Among them, 5 patients completed radiotherapy, and the appearance change of the breast was not obvious. All patients were followed up and underwent color ultrasound examination of the breasts, which showed that the flaps survived well. There was no recurrence of tumor or inflammation.Conclusions:Pedicled LTAP flap is an alternative operation scheme for partial breast reconstruction after breast-conserving surgery for breast cancer or lesion resection for non-lactation mastitis, especially for reconstructing of the lateral region. It has the advantages of simple operation, easy survival of the flaps, low complications, and relatively hidden incisions.

Objective:To explore the feasibility and cosmetic effect of repairing partial breast defects with pedicled lateral thoracic artery perforator(LTAP) flap.Methods:Pedicled LTAP flap was applied to repair partial breast defects in 12 patients from the Department of Breast Surgery of Shenzhen Maternal and Child Health Hospital of Southern Medical University from May 2019 to October 2020. Before the operation, the Doppler ultrasound was used to explore the perforator branch of the lateral thoracic artery in the 3rd to 4th intercostal area of the lateral thoracic wall, and a transverse or nearly transverse pedicled LTAP flap containing the perforator branch was designed. During the operation, the flap was elevated from the deep surface of the deep fascia, through which the lateral thoracic vessels were faintly visible. Then the vascular direction was determined with an audible Doppler blood flow detector. From the deep fat layer and the deep surface of the deep fascia, the pedicle with a width of about 1.2 cm and a thickness of about 0.5 cm was formed by careful dissection along the main vessel to the axillary direction. The flap was transferred to the breast defect after the epidermis of the flap was removed. The survival of the flaps was observed, and the reconstructed breast shape was evaluated.Results:A total of 12 patients were included. All patients were female, aged from 32 to 58 years old, with an average of 41.7 years. Among them, there were 8 cases of breast cancer and 4 cases of non-lactation mastitis. The lesions were all located in the lateral breast area. In this study, the tissue weight of tumor or inflammatory lesions was (78.3±5.1) g(56-92 g), and the LTAP flap with pedicle was (9.3±0.6) cm (8.2-12.5 cm)in length and (5.6±0.4) cm(3.8-7.2 cm) in width. In this group, 1 patient developed an infection of the donor site drainage entrance. After wound dressing and taking oral antibiotics, the wound healed quickly and healed well. Postoperative follow-up was conducted for 2-17 months, with an average of 8.2 months. There were 10 cases with good cosmetic effect and 2 cases with average cosmetic effect, and there were no patients with poor cosmetic effect. All patients were satisfied with the shapes of the reconstructed breasts. Among them, 5 patients completed radiotherapy, and the appearance change of the breast was not obvious. All patients were followed up and underwent color ultrasound examination of the breasts, which showed that the flaps survived well. There was no recurrence of tumor or inflammation.Conclusions:Pedicled LTAP flap is an alternative operation scheme for partial breast reconstruction after breast-conserving surgery for breast cancer or lesion resection for non-lactation mastitis, especially for reconstructing of the lateral region. It has the advantages of simple operation, easy survival of the flaps, low complications, and relatively hidden incisions.