Abstract: To comparatively study the effect of three roots talh(pulvis compositus) medicine and three roots granule medicine in the immune system. Materials and Methods: A total number of 32 healthy Wistar male rats weighing 180-220 grams were taken and equally divided into four groups: healthy group; small dose (0.85g/kg) of three roots talh medicine group(TRSD); high dose (1.7g/kg) of three roots talh medicine group(TRHD) and three roots granule medicine group at dose 1.3g / kg(TRGM). Medicine was given once every day for 42 days. At the end of the study, spleen index and thymus index were calculated and IL-2, IL-6, TNF-α, iNOS, NO levels in blood plasma and spleen using ELISA(Magellan 7.2) was determined and compared with healthy group. Result: There was no statistically significant increase in spleen index and thymus index between the healthy group and medicine groups(p>0.05). In blood plasma, there was no statistically significant difference in IL-2, IL-6, TNF-α, iNOS and NO between healthy and medicine groups (P> 0.05).
In spleen, there was significant increased in IL-2 in the high dose three roots talh medicine group in compare with healthy group (p<0.05). Similarly, IL-2, IL-6 and TNF-α was significantly increased in three roots granule medicine group in comapre with healthy group (P<0.05; P<0.05; P>0.001 respectively). There was no significant difference in iNOS and NO between healthy and medicine groups (p>0.05). Conclusions Three roots talh medicine and three roots granule medicine has active of immune system and three roots granule medicine has more activates the immune system compared to the three roots talh medicine.

Abstract Since ancient times, In traditional medicine has been widely used for Tan medicine, which make to natural raw material. Nowadays, this practice has been renewed, and researches are being conducted to determine their chemical component, mineral content and to explain their activating mechanism. Compounds with antioxidant activity contained in the pharmaceutical raw materials has very important benefits for metabolism, organ system function and to inhibit inflammation, oxidation reaction. In Mongolian traditional medical sources literature noted that bark (Larix sibirica Ldb) has activity such as anticoagulant, anti-cancer, anti-flu and to decrease arterial hypertension and also it was included to composition of "Tanchin-25" reception. Spectrometry and Rentgen fluorescence spectroscopy were used to identify chemical composition and content of mineral in the sample of Larix bark (Larix sibirica Ldb). According to the exploration, content of total flavonoid were determined 0.773%, and by mineral test, essential macro and micro elements that were obtained Ca (18.74%), Mg (8.31%), P (13.29%), K (2.49%), Na (0.675%), Cu-(375ppm), Zn(715ppm), Cr (68ppm) and V (17ppm), Co (5ppm).

Abstract: Mongolian traditional medicine has history of 5000 years and has been used medicinal plants, medicinal animal products, and minerals for prevention, treatment, more energy or strength. The woody parts, resins-colophony, and bark of the Siberian larch, belonging to the genus Pinaceae, are used in traditional medicine as a medicinal raw material. This paper purposed to reviews the chemical composition and pharmacological research of Siberian larch, a natural medicinal raw material that occupies 60.4% of Mongolia’s natural forest area and 78.7% of its reserves, and its use in Western and Oriental medicine. Method: The study of the chemical composition of Siberian larch, pharmacological research, and its use in Western and Eastern medicine was judged on the basis of sources, scientific articles, and textbooks. Result Alkaloids, flavonoids, various essential oils, polysaccharides, sesquiterpenes in the upper part of the Siberian larch, flavonoids such as quercetin, taxifolin, kempferol in Siberian larch wood, abietic acid in the solid part of Siberian larch resin, Siberian larch bark contains tannins (10-15%), lignin (46-47%) and polyphenols (38-39%). The woody part of the Siberian larch tree has antiviral activity against Herpes Simplex, high antioxidant properties, and is used to prevent and treat allergic diseases. The flavonoids in larch resin reduce the risk of cardiovascular disease and cancer. Larch bark inhibits E.coli bacteria.

Purpose: To study the effect of Three roots granule medicine with compound medicines ingredient of Asparagus cohinchinensis, Polygonatum odoratum and Polygonatum sibiricum on acute toxicity test and genotoxicity tests. Methods: In toxicity study of Three roots granule medicine, by acute toxicity test were observed general status of animals, body weight changes, signs of poisoning and death for 14 day and determined the maximum tolerated dose, by Ames test, mouse bone marrow polychromatic erythrocyte micronucleus test and mouse sperm deformity test were determined genotoxicity effect. The data were analyzed through SPSS 19.0. Results and Conclusions In the result of toxicity study, three roots granule medicine was MTD>15g/kg, no acute toxic activity, did not induce mutagenic effect in Ames test and was negative in mouse bone marrow polychromatic erythrocyte micronucleus test and mouse sperm deformity test. Three roots granule medicine has no acute toxicity effect, no genotoxicity effect and safety. We as regard as in future can continuously study to the other pharmacology study of three roots granule medicine.