1.ЭКСГУМАЦИ ШИНЖИЛГЭЭГЭЭР ГЭМТЭЛ ИЛРҮҮЛЭХЭД ЛЕПЕНИЙ БУДГИЙН АРГЫГ ХЭРЭГЛЭХ НЬ
Ariunzul B ; Ganbold Ts ; Enkhjin B ; Tuya N
Innovation 2017;11(2):39-40
BACKGROUND. When the body of musty and changed, a long time in the water can be difficult to identify soft tissue injury. For detecting hemorrhage in changed tissues we are using the method named after German doctor G.Lepchne, born in 1887. The method, based on the peroxidase activity of hemoglobin pigment. It is recommended to stain frozen slices.Erythrocytes and hemoglobin are colored in a dark brown color. Reagent: 2 ml 0.6% benzidine solution, 0.5 perhydrol, 4.5 ml of 70% ethyl alcohol
Since 20148 we have been using the method in four cases. The result was useful for detecting injuries.
2.УРСГАЛ УСНЫ ЧИМЭЭГ ЭМЧИЛГЭЭНД АШИГЛАХ БОЛОМЖ
Dariimaa G ; Lkham-Edrene B ; Enkhjin B ; Nomin G ; Davaadulam E
Innovation 2017;11(2):103-107
In this study we aimed to check a hypothesis about water sound (WS) effect can
stimulate parasympathetic nervous system. A case-control study design was used. The
students in control group did not listen WS, whereas students in case groups had been
influenced under WSE by 3 different ways, including audio, video and shower. Data
base had been obtained by performance of stress test, measurements of systolic and
diastolic pressure, and counts of pulse. For statistical analysis, IBM SPSS 20.0 and Graphpad
prism 7.0 were used. Interestingly, 92% of total students had stress. There were no
differences between groups before WS effect. After WS effect, ANOVA test detected
differences in diastolic pressure (ANOVA, F=6.209, p=0.001), stress score (F=15.21,
p<0.001). Paired test was utilized to compare mean of values between before and post
WS measurements, finding SP (p=0.001), SS (p<0.001) categories. It showed an effective
WS influence. Chi-square test indicated that stress level of all students decreased significantly
(p<0.001). But there were no differences between 3 methods of water therapy. In
conclusion, WS has an effect of stimulation in immune system, useful for some diseases,
in which parasympathetic system decrease and sympathetic system increases.
3.Analyzing an evidence-based prescription in the “Man ngag rin chen ‘Byung”
Enkhjin G ; Gerelmaa B ; Tsetsegmaa S ; Bold Sh
Mongolian Pharmacy and Pharmacology 2020;17(2):59-63
Abstract:
The basis of the development of traditional medicine in the XXI century is to study the old medical books and scriptures created by ancient Mongolian doctors and scientists of previous generations. For example, it is important to deepen the scientific understanding of “Man ngag rin chen ‘byung” written by Mongolian doctor ‘Jam dpal chos kyi bstan ‘dzin phrin las in Tibetan, and to understand the content and charms of the Man ngag rin chen ‘byung.
Purpose:
Sampling and analysis of experimental prescriptions in the “Man ngag rin chen ‘byung” written by Mongolian doctor ‘Jam dpal chos kyi bstan ‘dzin phrin las in Tibetan.
Research methods:
Methodologies of ancient textbook analysis and check list of textbook were used.
Conclusion
In the concluding remarks of the “Man ngag rin chen ‘byung” written by Mongolian doctor ‘Jam dpal chos kyi bstan ‘dzin phrin las, it was stated that there are many medicinal raw materials in the practice. He mentioned that: “I have summarized the benefits of medical literature written by the ancient doctors of Mongolian medicine to facilitate for people to learn.” An analysis of this sentence reveals that in the current understanding of medical science, it is evidence-based practice (EBP).
4.Comparison of Sysmex UF-5000 with urine culture for the diagnosis of urinary tract infections
Otgontungalag D ; Battulga M ; Bolortungalag B ; Munkhzaya D ; Bayanmunkh B ; Erkhembayar O ; Oyuntsetseg D ; Enkhjin B ; Bolor A
Health Laboratory 2020;12(2):5-10
Introduction:
Urinary tract infections (UTI) are at the second place in the frequency of all causes of infection after respiratory ones. The UTI requires appropriate antibiotic treatment. 85% of UTI predictive antibiotic treatment without confirmation by bacteriological analysis. This is one of the major causes of drug resistance, especially in K.coli. Urine bacteriological tests do not show bacterial culture in all cases where the number of bacteria in the urine exceeds the reference level. Therefore, there was a need to establish criteria for urine bacteriology test based on the results of urine sediment analysis.
In 20I7, a new fully automated Sysmex UF-5000 urine sediment analyzer was installed in the laboratory department of Medipas Hospital. The features of this analyzer include counting the number of bacteria in the urine, distinguishing between gram-positive and negative, homogeneous and mixed forms, and counting the formed elements in the urine. This feature made it possible to compare the number of bacteria and leukocytes in the urine with the results of urine bacteriology tests.
Goal:
Determine the relationship between the number of white blood cells and bacteria in the urine measured by the Sysmex UF-5000 urine sediment analyzer and the results of the urinary bacteriological test.
Objectives:
Compare the number of urine bacteriaand leukocyte measured by using the Sysmex UF-5000 urine sediment analyzer with the urine bacterial culture, and calculate the correlation.
Materials and methods:
The study is analytic cross-sectional study, analyzed the results of a total of 159 people who analyzed a urinalysis and urine bacteriological test at the Medipas Hospital Laboratory in 2017-2019 years.Urine samples were collected in a 100 ml, disposable sterile container in accordance with the instructions for taking urine midstream.Urine analysis was performed within 2 hours of sampling with a fully automatic urine sediment analyzer Sysmex UF-5000 Japan. Urine bacteriological analysis was performed on a lul sterile loop of urine specimens, inoculated into 5% blood agar from Hungary's BioLab, Sabouraud agar, and Chromogen agar from Biomerieux France, and incubated for 24 hours in an incubator at 37°C. Bacterial identification and antibiotic susceptibility tests were analyzed using the "Vitek-2" analyzer from the manufacturer Biomerieux France. Bacterial and leukocyte counts data measured by the Sysmex UF-5000 analyzer and urinary bacteriological analysis data were performed using SPSS23 software.
Results:
A total of 159 urine samples were tested for bacteriological analysis, of which 81 (50.9%) were bacteria over 105 CFU/ml or urine positive culture UTIs, 78 (49.1%) were nonsignificant bactcruria and urine negative culture.The average number of bacteria measured in the urine of 81 samples with urine positive culture above 105 CFU/ ml was 46491/ul (1168-100000 BACT/ul).
The average number of bacteria measured by the urine sediment analyzer of 78 samples with urine negative culture was 2645 BACT/ ul (2-57280 BACT/ul). To calculate more accurately estimate the average number of bacteria in 81 urine specimens with positive culture, the average number of bacteria in 17 (21%) samples was 4753 BACT/ul, measured in relatively low bacteria numbers of 1168-9450BACT/ul. The average leukocyte number in the urine of 81 samples with positive culture was 472.2 WBC/ul, and the average leukocyte number in the urine of 78 samples with negative culture was 87.7 WBC/ul.There is a strong correlation between the number of bacteria measured by the urine sediment analyzer and urine bacterial positive culture, which is 0.8 or statistically significant (p<0.001).The correlation coefficient of the number leukocytes measured by the urine sediment analyzer with in the urine positive cultureof bacteriological tests was 0.6 or moderately of statistically significant (p=0.005).There is a statistically significant relationship (p=0.001) between the number of bacteria in the bacterial positive culture population and the number of leukocytes.
Discussion:
Of the 81 cases of urine bacterial positive culture, 78 (96%) were female, indicating a high prevalence of UTI among women. According to the results of the Fabio Manon's study, the number of leukocytes in the urine is 160-340 WBC/uL and the number of bacteria is 15000-30000 BACT/u,L in the case of UTI, which is approximate results compared to the our study results.Based on the results of the urine sediment analysis, indications for a urine bacteriological test should be made.
Based on the results of urinary bacteriological tests, the choice of antibiotic treatment is the best treatment for urinary tract infections and a way to prevent of antibiotic resistance to UTI.
Conclusions
The number of bacteria measured by a Sysmex UF-5000 urine sediment analyzer is directly related to the bacterial culture urine bacteriological test. If the number of bacteria in the urine is measured above 4753 BACT/ul, it can be considered as an indication for urine bacteriological analysis. Although the number of leukocytes in the urine measured by the Sysmex UF-5000 urine analyzer is moderately correlated with bacterial culture in urine bactcriologucal tests, it is a key indicator of the degree of inflammation of the urinary tract.
5.Interdependence of hepatitis B and C virus infection with M2BPGi marker
Battulga M ; Erdembayar O ; Munkhzaya D ; Bayanmunkh B ; Oyuntsetseg D ; Enkhjin B ; Undarmaa G ; Otgontungalag D ; Bolor A
Health Laboratory 2021;13(1):21-25
Introduction:
Over 800,000 people in the world contract HCC each year and approximately 700,000 die from the disease. HCC is the 6th most common cancer in the world. HCC is the 3rd leading cause of cancer deaths in the world. 2/3 of liver cancer deaths are caused by hepatitis. In the U.S, HCV infection is the more common cause of HCC, while in Asia and Africa, HBV is more common. Mongolia ranks first in the world in mortality from liver cancer, indicating the need for early detection and treatment of cirrhosis. Sysmex Corporation has introduced for HISCL series analyser, a new cirrhosis marker M2BPGi of non-invasive, blood-testing. In 2016, the test was introduced
at Medipas Hospital in Orkhon province. It is possible to study the advantages and significance of the marker for use in clinical practice.
Materials and methods:
From a total of 385 patients who underwent M2BPGi marker testing in 2016-2017Medipas hospital laboratory, data from a total of 283 patients tested for hepatitis B and C virus and M2BRGi markers were selected. A comparison of age, sex, and test parameters of a total of HCVab and HBsAg positive 172 patients tested for Total bilirubin, GPT, GOT, GGT, AFP and M2BPGi. HCV Ab, HBsAg, AFP, M2BPGi markers were analyzed by SysmexHISCL-5000 fully automated immunological analyzer, Liver function tests were performed with a fully automatic biochemical analyzer JEOL Biomajesty BM6010/C.
Results:
Of the M2BPGi marker tested 283 patients 94 (33%) were infected with the C virus, 78 (28%) were with the B virus,11 (4%) were co-infected with B and C viruses, 100 (35%) no any viral infection. Of the 172 patients diagnosed with hepatitis B and C virus infection, 97 (56%) were male, 75 (44%) were female. In terms of age, 72% of the population is over 45 years old.
Of the 172 patients, 115 (67%) had M2BPGi marker abnormal or > 1.0 COI. Of the M2BPGi marker abnormal patients, 47 (41%) were infected with the B virus and 68 (59%) with the C virus. In terms of age, 27.7% of hepatitis B patients and 10.3% of hepatitis C patients were under 45 years of age, 72.3% of hepatitis B patients and 89.7% of hepatitis C virus patients were over 45 years of age.
Hepatitis B and C viruses are slightly more common in men than in women. The majority of patients infected with the hepatitis virus over the age of 45. The majority of patients with hepatitis virus have abnormal liver function. Increased M2BPGi markers in people under the age of 45 with hepatitis B virus infection are relatively higher for hepatitis B virus infection than for C virus infection.
Conclusions
The M2BPGi marker was abnormal in 67% of hepatitis virus infected patients. It has been observed that the probability of an increase in M2BPGi marker is slightly higher in hepatitis C virus infection than in hepatitis B virus infection.