Objective: To determine the antibody titer in the serum of allergic rabbits after the injection of 1, 3-di-caffeoylquinic acid contained in Shuanghuanglian injection.Methods: The complete antigen was prepared by incubating the suspected small molecular hapten 1, 3-di-caffeoylquinic acid contained in Shuanghuanglian injection with the serum from the normal rabbits.The specific antibody was obtained in the immunized rabbits.The antibody titers of antiserum were measured by ELISA kits.Indirect competitive ELISA was used to determine serological specificity, and the obtained data was used to plot the inhibition curves.The content of IgE antibody in the antiserum of rabbits was detected by rabbit immunoglobulin E (IgE) ELISA kits.Results: The antibody titer (A) of 1,3-di-caffeoylquinic acid was 2 times higher than that of the negative control, which indicated its potential allergenicity.The regression equation was I=0.170 6 lg C + 0.317 5 , which was with the correlation coefficient of r=0.985 4 , the detection limit of IC 10 =57.40 μg·ml-1 and the half inhibitory concentration of IC 50 =8.732 0 mg·ml-1.Furthermore, the exogenous IgE antibody was produced in the rabbits.Conclusion: The results indicated that the hapten substance 1,3-di-caffeoylquinic acid in Shuanghuanglian injection was allergenic under the present experimental conditions.

OBJECTIVE: To explore the molecular mechanism underlying the inhibitory effects of aspirin against human breast cancer cell proliferation through bioinformatics analysis. METHODS: Drug Bank 5.1.3 was searched to identify direct protein targets (DPTs) of aspirin, and the protein-protein interaction (PPI) network of the DPTs was constructed online using STRING and the signaling pathways involved were identified. The genetic alterations of 6 DPTs associated with human breast cancer was analyzed and visualized by cBio Portal and OncoPrint, respectively. The transcriptomic data of breast cancer and normal tissues were downloaded from TCGA database, and the overexpressed genes were analyzed by DECenter. The intersection between the genes associated with the DPTs obtained by STRING analysis and the differentially over-expressed genes in TCGA was determined to confirm the candidate DPTs as a potential target of aspirin, and GO functional enrichment analysis was performed using Gene Ontology. The potential targets of aspirin against the proliferation of human breast cancer cells were verified by Western blotting. RESULTS: Eleven DPTs of aspirin were identified. KEGG pathway enrichment indicated that 6 genes (EDNRA, IKBKB, NFKB2, NFKBIA, PTGS2 and TP53) were associated with the occurrence and development of cancer. A total of 10 220 differentially expressed genes were identified from the TCGA database, and among them 4 genes (, , , ) were found to be the potential targets for aspirin. These genes were involved mostly in the regulation of cell cycle and cell division. Western blotting showed that aspirin could down-regulate the expression levels of several pivotal proteins that regulated cell cycle and cell division, including , , and . CONCLUSIONS , , and may be potential targets for aspirin to inhibit the proliferation of human breast cancer cells, by affecting the progress of cell cycle and cell division.

OBJECTIVE： To study the improvement effects of Shenshao yiqi yangxue granule on experimental aplastic anemia （AA） in mice and the preventive and therapeutic effects of it on myelosuppressive in mice. METHODS： Totally 72 mice were randomly divided into blank group （normal saline）， model group （normal saline）， positive control group （Yixuesheng capsule， 0.625 g/kg in content）， Shenshao yiqi yangxue granule low-dose， medium-dose and high-dose groups （1.26， 7.56， 15.12 g/kg by crude drug）， with 12 mice in each group. Except for blank group， other groups were given 60Co-γ irradiation combined with intraperitoneal injection of CTX to induce AA model. After modeling， they were given relevant medicine intragastrically once a day， for consecutive 7 d. After 6 days of administration， the mice were placed on a treadmill for exhaustion test， and the exhaustion distance and exhaustion time were measured. After 7 days of administration， the changes of peripheral blood （the number of RBC， WBC， PLT and content of HGB） were determined of mice in each group. The percentage of reticulocyte （Ret） was measured after blood smear， and the number of bone marrow nucleated cells （BMNC） was determined after bone marrow smear. Another 90 mice were collected， grouped and given relevant medicine as above， with 15 mice in each group. They were relevant medicine intragastrically once a day， for consecutive 12 d. After 3 days of administration， except for blank group， other groups were given 5-fluorouracil intraperitoneally to induce the bone marrow suppression model at the beginning of medication. After medication， peripheral hemogram， Ret and the number of BMNC were determined in each group. RESULTS： Compared with blank group， the exhaustion distance and exhaustion time of AA model group were significantly decreased （P＜0.01）， while the number of RBC， WBC， PLT and content of HGB， Ret and the number of BMNC were decreased significantly （P＜0.05 or P＜0.01）. Compared with model group， the exhaustion distance and exhaustion time of positive control group and Shenshao yiqi yangxue granule medium-dose and high-dose groups were increased significantly （P＜0.05 or P＜0.01）； the peripheral hemogram indicators， Ret and the number of BMNC were increased significantly in positive control group and Shenshao yiqi yangxue granule high-dose group， and Ret of Shenshao yiqi yangxue granule medium-dose group was increased significantly （P＜0.05 or P＜0.01）. In bone marrow suppression model， compared with blank group， the number of WBC， RBC and PLT， Ret， the number of BMNC were decreased significantly in model group （P＜0.05 or P＜0.01）. Compared with model group， the number of WBC， RBC and PLT， Ret and the number of BMNC in peripheral blood were increased significantly in positive control group and Shenshao yiqi yangxue granule high-dose group， and the number of RBC， Ret and the number of BMNC were increased significantly in Shenshao yiqi yangxue granule medium-dose group （P＜0.05 or P＜0.01）. CONCLUSIONS： Shenshao yiqi yangxue granule has certain improvement effects on AA in mice， and can prevent and treat myelosuppression in mice.