Objective To elucidate the pathogenesis of achalasia, Benzyldimethyltetradecylammo-nium chloride ( BAC) was used to create amyenteric cat achalasia models. Methods Twenty-four cats were randomized into two groups and injected respectively with BAC in models or saline in controls into the distal esophagus under endoscopy. After 8 weeks, manometry, biopsy with HE stain, acetylcholinesterase activity and immunohistochemistry were studied. Results Manometrically, the pressures of LES in cat models showed higher than those of the controls (P

Objective To investigate the therapeutic effect transferring acetylcholinesterase (AChE) gene into experimental cat achalasia. Methods Thirty six cats were divided into 4 groups.Group 1 was served as healthy control.The cats in other three groups were injected with benzaikoniumchloride(BAC) into the lower esophageal sphincter(LES) under endoscopy to establish achalasia model. Then the cats were injected with normal saline (group 2 as experiment control),adenoviral vectors containing green fluorescent protein (AdGFP, group 3) or adenoviruses containing full length AChE T cDNA (group 4) by the same way 8 weeks later. RT PCR and immunohistochemistry was performed to detect the expression of the AChE 10 days later.Manometry was carried out to check the pressure of LES. Results RT PCR amplification results indicated that AChE T fragment corresponding to target gene was presented in group 4. The expression of AChE T mRNA was positive and AChE protein could be detected by immunohistochemistry in the LES of cats treated with AdAChE T. In addition AChE activity increased significantly in group 4 than that in group 3 and group 2( P

Objective To study the therapeutic effects of Bioenterics Intragastric Balloon (BIB) on obesity under gastroscopy.Methods Data of 47 patients treated with Bioenterics Intragastric Balloon under gastrscopy were reviewed from July,2010 to May,2011.Results Weight loss ( mean 15.4 kg ) was successfully achieved in all the patients during 6 months.BMI decreased by 3.2-6.4 kg/m2 ( mean 4.7 kg/m2 ).There was no serious side effect with a better result for obesity according to the follow-up.Conclusion BIB is effective for obesity for noninvasiveness,stable speed of weight loss and less pain.