Objective The purpose of this paper is to study PGM1 genotyping by PCR RFLP.Method 300 unrelated individuals of Han were genotyped using PCR RFLP. The target amplificaton products of extron 4 and 8 of PGM1 gene were digested by Bgl II and Nla III respectively.The digested DNA fragments were typed by PAGE.Result This PGM1 RFLP system can discriminate 9 genotypes with Dp of 0 7450 in Han population.Compared with conventional PAGIEF, 1+2- and 1-2+ cant be differentiated and the rare genotypes also cant be detected by this method.The advantage of this method was PGM1 genotyping successfully in bloodstains stored for 25 years and with 0 1ng genomic DNA.PGM1 RFLP method is useful for forensic identification.

Objective: To study the effect of 30-min nap at three low points of the rhythm during 40-hours sleep deprivation on continuous adding. Methods: Eight young subjects with normal sleep-wake habits were subjected to nap and total sleep deprivation conditions at an interval of 2 weeks. The whole experiment was from 6:00 of the first day to 22:00 of the second day, totally 40 hours. There were three times of nap which lasted 30 min every time, 13:00(the first day), 1:00 and 13:00(the second day). Continuous adding was given separately at the time before experiment,13:00 and 18:00 of the first day, 1:00,6:00,13:00,18:00 and 22:00 of the second day.and 2 days after the experiment;reaction times and wrong numbers were recorded. Results: Whether sleep deprivation with or without naps, the reaction time was prolonged.Compared with total sleep deprivation, the reaction time was shortened significantly at SD24, SD31 and SD40 when naps were introduced. Whether sleep deprivation with or without naps, the number of wrong answers did not change. Conclusion:Nap has positive effects during sleep deprivation.

Objective:To study the effect of 30-min nap at three low points of the rhythm during 40-hour sleep deprivation Method:Eight young subjects having normal sleep-wake habits were subjected to nap and total sleep deprivation conditions at an interval of 2 weeks The whole experiment was from 6:00 of the first day to 22:00 totally 40 hours The typing workload was given There were three times of naps, which lasted 30 min every time, at 13:00 (the first day), 1:00 and 13:00 (the second day) Visual event related potentials were measured at 1:00 and the end Symbol recognizing task was given before the experiment, after each nap, at the end of it, and two days after Results:In the symbols recognizing task, whether sleep deprivation with or without naps, the reactive time prolonged, the number of right decreased, the number of lose increased Compared with total sleep deprivation, the reactive time was shortened apparently while the number of right and lose did not change when naps were introduced Whether sleep deprivation with or without naps, the latency of P300 was prolonged, the number of right decreased while the amplitude did not change Compared with total sleep deprivation, the latency was shortened apparently while the number of right and amplitude did not change when having naps Conclusion:Nap has positive effects during sleep deprivation

Objective To investigate the association between the signaling pathways of MCP-1-pCDH-GFP-trans?fected cells and deep venous thrombosis (DVT). Methods The cultured human umbilical vein endothelial cells (HUVECs) were tested by immunofluorescence and co-immunoprecipitation methods. The constructed MCP-1 over-expression/interfer?ence vector, and the change of transcription profile were detected by microarray assay and biological information technology analysis. Results MCP-1 over-expression/interference vector MCP-1-pCDH-GFP/MCP-1-LMP shRNAmir1 was con?structed and HUVECs were transfected. According to the microarray analysis we found that there were 18 down-expressed signaling pathways and 7 up-expressed signaling pathways in MCP-1-pCDH-GFP-transfected cells. There were 60 down-expressed signaling pathways and 15 up-expressed signaling pathways in the MCP-1-LMP shRNAmir1 transfected cells. Conclusion Signaling pathways of MCP-1 plays an important role in DVT formation,which may provide us a new way to study molecular mechanism of DVT.

Objective:To investigate the expression of c-Fos protein after total and partial sleep deprivation(SD) of 96 hours.Methods:SD was induced in the male Sprague-Dawley rats by housing them on the small platforms over water.Controls were housed in the normal cage(CC) or on the large platforms over water(TC).The effects of total and partial 96h SD on the expression of c-Fos protein were examined by immunocytochemisty.Results:SD made Fos protein express differently in the different fields of the brain.The expression of Fos protein of partial SD was wider and lighter than of total SD.Conclusion:Compared with total SD,partial SD has lighter stress.

Objective To build rat DVT inferior vena cava partial stasis (narrow) model, to detected the expression ofβ2-GP1, VEGF and TF in rat blood, and to investigat the correlation betweenβ2-GP1, VEGF and TF with DVT. Meth?ods SD rats (n=70) are divided into control group (n=10), sham operation group (n=30) and the model group (n=30) ran?domly and DVT model was built by the inferior vena cava partial stasis (narrow) after 2 h, 8 h and 24 h respectively. In each time point, ten rats were taken in each group, inferior vena cava blood were collected whileβ2-GP1, VEGF and TF expres?sion were detected by ELISA. Results In rat experiment, compared with control group, there was no significant change in?expression of β2-GP1, VEGF and TF in sham operation group (P > 0.05). Levels of β2-GP1, VEGF and TF were in?creased at the 2nd hour and 8th hour then peak at the 24th hour which was higher than those in the 24th hour control group and in Sham group and it was also higher than those in the 2nd hour and the 8th hour in model group with statistical signifi?cant difference (P<0.01). Conclusion Based on the above experimental data, in rat DVT formation process, β2-GP1, VEGF and TF may play an important role in promote DVT formation.

Objective To study and establish a high quality digital 3D anatomical modeling of the mandible with full teeth.Methods A set of accurate digital models of standard anatomical specimens of mandibular teeth were obtained by laser scanning, and the 3D mandible model was reconstructed by CT scan data;then, a registration deformation method based on the geometry and image anatomical landmark was employed to do the registration of each tooth to the mandible model, and finally the tooth enamel , dentin, periodontal ligament were generated .Results A high quality digital 3D anatomical modeling of the mandible with full teeth was built , each tooth had detail crown and whole root , the distinction between the enamel , dentin, periodontal ligament , and any anatomical regions can be zoomed and rotately displayed . Conclusion The digital 3D anatomical modeling of the mandible with full teeth has realistic 3D imaging view and convenient teaching-learning function , and has tremendous apllication futures in the stomatology , maxillofacial and other medical departments .

The rehabilitation of a 19-year-old male with a congenital ventricular septal defect who suffered a haemorrhagic stroke from a ruptured mycotic aneurysm, secondary to infective endocarditis is described in this case study. This case study illustrates the application of the International Classification of Functioning, Disability and Health (ICF) model, and Kawa model in the structuring of a holistic, interdisciplinary team management. Application of both models provide clear communication within the interdisciplinary team and aids the team to understand the contextual factors in the interaction of the patient's rehabilitation journey.

A case study highlighting the complexity in the management of newly diagnosed atrial fibrillation in an elderly female with recurrent falls and a lack of mental capacity. This demonstrates a delicate balance between reducing thromboembolic phenomenon and bleeding risks.