1.Study on the infection of primary tupaia hepatocytes with hepatitis B virus in vitro
Jingjing ZHANG ; Guang YANG ; Chun YANG ; Yanrong HAO ; Ji CAO ; Chao OU ; Yuan LI ; Kechen BAN ; E.blum HUBERT ; Jianjia SU
Chinese Journal of Microbiology and Immunology 2008;28(11):984-988
Objective To provide a better cell model of closely nature infectious state for further research of hepatitis B virus(HBV). Methods Primary tupaia hepatocytes were isolated by the two-step perfusion method. The hepatocytes were then infected with purified serum from patients with hepatitis B. DNA and RNA isolated from the hepatocytes were detected with Southern blot and Northern blot. HBsAg in supernatant was tested by immunohistochemical method. Results cccDNA, pgRNA and sgRNA could be detected by Southern blot and Northem blot, and strong signals could be seen from day 7 to day 14 post-in-fection. The S/CO value of HBsAg in supernatant decreased from day 1 to day 5 and then increased after 5 day. Conclusion Primary tupaia hepatocytes are competent for infection with HBV. HBV can stably repli-cate and express in HBV-infected tupaia hepatocytes.
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