Adult ; Aged ; Breast Neoplasms ; metabolism ; pathology ; Carcinoma, Ductal, Breast ; metabolism ; pathology ; Estrogen Receptor alpha ; metabolism ; Estrogen Receptor beta ; metabolism ; Female ; Fibroma ; metabolism ; Humans ; Lymphatic Metastasis ; Middle Aged ; Receptor, ErbB-2 ; metabolism

Phytoremediation effectiveness and remediation costs are driving factors of this project. Full utilization of plant resources after their being used for phytoremediation is an unsolved problem. GC/MS technique was used to investigate the volatiles of the flowers from Elsholtzia argyi (PFE1: Purple Flower Elsholtzia) and their variation (WFE: White Flower Elsholtzia), naturally growing in Pb/Zn mined area, and Elsholtzia argyi (PFE2: Purple Flower Elsholtzia), naturally growing in Jiuxi uncontaminated agriculture soil. Seventeen compounds constituting 86.88% of total essential oils were identified in PFE1, with 2,6-octadienoic acid,3,7-dimethyl-methyl ester being the main constituent (63.30%). Sixteen compounds accounting for 95.32% of total essential oils were identified in WFE, with caryophyllene being the main component (55.02%). Compared to PFE1, PFE2 contains lower level of 2,6-octadienoic acid,3,7-dimethyl-methyl ester (31.76%), which is the main constituent in the total essential oils of PFE2. Caryophyllene is the main ingredient of flavor. Elsholtzia ketone was identified in all the three Elsholtzia plants. It can be concluded that the selected Elsholtzia argyi plants can be exploited on their versatile uses as fragrances and antiseptics due to the perfume ingredient and antibacterial components existing in their essential oils.
Agriculture ; methods ; Biodegradation, Environmental ; Conservation of Natural Resources ; Flowers ; classification ; metabolism ; Lamiaceae ; classification ; metabolism ; Oils, Volatile ; analysis ; metabolism ; Plant Extracts ; analysis ; metabolism ; Species Specificity

The afferent connections of the lateral hypothalamic nucleus in the rat werestudied with retrograde horseradish peroxidase(HRP)method using microinjection andmicroelectrophoretic technique.The microinjection syringe needle or microelectrophoretic pipette was insertedvertically into the brain or in inclined direction through the cerebellum.The results were as follows:Following HRP introduced into the lateral hypothalamic nucleus,labeled neuronswere found in the anterior hypothalamic nucleus,the paraventricular nucleus,thedorsal raphe nucleus,the locus coeruleus and substantia grisea periventricularis inall cases.In most cases,labeled cells were also observed within the lateral septal nucleus,the interstitial nucleus of stria terminalis,the medial preoptic nucleus,the lateralpreoptic nucleus,the supraoptic nucleus,the ventromedial hypothalamic nucleus,themedial and central amygdaloid nuclei,the nucleus reunius,the interpeduncular nuc-leus,the nucleus parabrachialis dorsalis,the nucleus parabrachialis ventralis,and thenucleus tractus solitanii.In a few cases,retrograde labeling of neurons was observed within the medialseptal nucleus,pars medialis of the nucleus medialis thalami,the supramammilarynucleus and nucleus raphe medianus.In certain cases,a small number of cells could be found in the dorsal tegmentalnucleus of Gudden and the nucleus dorsalis nervi vagi.Among those nuclei,the locus coeruleus,the supraoptic nucleus,the paraventri-cular nucleus and the dorsal parabrachial nucleus were labeled bilaterally.

Bone Neoplasms ; pathology ; Chondrosarcoma, Mesenchymal ; pathology ; Dura Mater ; pathology ; Humans ; Meninges ; pathology

Acute Disease ; Adult ; Bridged-Ring Compounds ; poisoning ; Female ; Humans ; Male ; Middle Aged ; Rodenticides ; poisoning

Carcinoma ; pathology ; Humans ; Paranasal Sinus Neoplasms ; pathology

Breast Neoplasms ; diagnosis ; genetics ; pathology ; Carcinoma, Ductal, Breast ; diagnosis ; genetics ; pathology ; Centromere ; genetics ; Chromosomes, Human, Pair 17 ; genetics ; Early Detection of Cancer ; methods ; Female ; Gene Amplification ; Gene Dosage ; Genes, erbB-2 ; Humans ; Immunohistochemistry ; In Situ Hybridization ; standards ; Receptor, ErbB-2 ; genetics

Objective To investigate the cell-type-specific enhancer (CTSE) in HPV16 and its variation in cervical carcinoma. Methods CTSEs were detected by polymerase chain reaction (PCR) in 58 cervical carcinoma from Shaanxi province; in addition variation of CTSEs was analyzed through single-strand conformation polymorphisms (SSCP). Results HPV16 CTSEs were detectable in 34 of 58 (57%) specimens and mutant rate was 41%(14/34) and the main mutations of chosen randomly variant CTSE (CTSEv) happened at YY1 binding sites in addition to glucocoticoid response elements (GRE). Conclusion CTSE in some specimens of Shaanxi province was obviously different from that in HPV16 wild type and variant CTSE might affect the transcriptional regulation of LCR on viral P97, which regulates over-expression of viral oncogenes in cervical carcinoma.

Animals ; Apoptosis ; Hippocampus ; pathology ; Male ; Neurons ; pathology ; Physical Conditioning, Animal ; Rats ; Rats, Sprague-Dawley ; Stress, Psychological ; pathology