Objective To compare the efficacy of isophane protamine biosynthetic human insulin premixed 30R( Novolin 30R) and biosynthetic human insulin ( Novolin R) combined with protamine biosynthetic human insulin (Novolin N) in the treatment of gestational diabetes mellitus and the patients compliance .Methods According to the digital table ,40 patients with gestational diabetes mellitus were randomly divided into A group ( 20 cases ) and B group(20 cases).A group was treated with Novolin 30R,while B group was given Novolin R combined with Novolin N.The blood glucose levels,the incidence of hypoglycemia during hospitalization ,clinical efficacy,security,conven-ience of insulin injection and compliance were compared and evaluated in the two groups .Results In A group after treatment,the compliance rate was 70.00%(14/20),that in B group was 75.00%(15/20),there was no significant difference(χ2 =0.125,P>0.05).In A group,the incidence rate of hypoglycemia was 10.00%(2/20),that in B group was 5.00%(1/20),there was no significant difference (χ2 =0.360,P>0.05).After treatment,the fasting blood glucose,postprandial 2 hours blood glucose levels in the two group were decreased significantly ( t =5.883, 5.248,6.856,7.549,all P<0.05),but the differences were not statistically significant between the two groups after treatment [(4.9 ±1.0)mmol/L vs.(4.8 ±1.2)mmol/L,(6.6 ±1.6)mmol/L vs.(6.3 ±1.8)mmol/L,t=0.085, 0.553,all P>0.05].In A group,the incidence rate of irregularly injected insulin was 1.23%(4/324),which was significantly lower than 3.58%(23/642) in B group(χ2 =4.37,P<0.05).Conclusion Novolin 30R program and Novolin R combined with Novolin N program can effectively control the glucose of gestational diabetes ,the clinical re-sults are satisfactory and relatively safe ,but the former is more acceptive by the patients .

Objective To study effects of Tongxieercaofang (TXECF) on levels of IL-2 in serum and NO, NOS in intestinal mucosa tissue of rats with ulcerative colitis, and further to research the mechanism of' restoreing ulcer of TXECF. Methods Ulcerative colitis model was established by immunization and stimulus method partly. 50 rats were divided into 5 groups randomly:normal group, model group, TXECF group, Tongxieyaofang (TXYF) group, Bupiyichangwan (BPYCW) group. Then the effect of TXECF on levels of IL-2 in serum and NO, NOS in intestinal mucosa tissue were observed. Results In successful model group, IL-2 in serum was much lower than that of normal group (P

The influences of varied concentrations of AG on the 8H-TdR(3H-Thymidine ) incorporation inhibition rate and the release of PGI2 and TXA2 were studied in cultured human and rat lymphocytes . The results indicated that the AG increased the 3H-TdR incorporation inhibition rate on the concentrations of 1.5? 10-7mol/L and 3?10-7mol/L. Using the RIA method, authors find that AG on the concentrations of 4 ? 10-7mol/L, 4 ? 10-3mol/L and 4 ? 10-11mo/L can significantly decrease the cultured lymphocytes TXA2 release while has no clear influences on PGI2 release

Objective: To investigate the epidemiological characteristics of newly reported HIV/AIDS cases at ages of 50 years and older in Zhengzhou City from 2017 to 2021, so as to provide the evidence for formulating the AIDS control measures among the elderly. Methods: Epidemiological data of newly reported HIV/AIDS cases at ages of 50 years and older in Zhengzhou City from 2017 to 2021 were collected through the Zhengzhou Municipal Intelligent Public Health Management System. The epidemiological characteristics including population distribution, transmission route and route of detection were analyzed using a descriptive epidemiological method. Results: A total of 533 newly reported HIV/AIDS cases at ages of 50 years and older were diagnosed in Zhengzhou City from 2017 to 2021, accounting for 25.94% of all HIV/AIDS cases. There were 400 male cases and 133 female cases, with a male-to-female ratio of 3.01∶1, 288 cases at ages of 50 to 59 years (54.03%), 467 cases with an educational level of junior high school and below (87.62%), 391 cases with household registered residence in Zhengzhou City (73.36%), and 333 farmers (62.48%). Sexual contact was the main route of transmission (524 cases, 98.31%), and HIV/AIDS cases were predominantly detected by medical institutions (305 cases, 57.22%), followed by counseling and testing (167 cases, 31.33%). The proportion of HIV/AIDS cases diagnosed by medical institutions appeared a tendency towards a decline in Zhengzhou City from 2017 to 2021 (χ2trend=10.953, P=0.001), while the proportion of HIV/AIDS cases diagnosed by counseling and testing showed a tendency towards a rise (χ2trend=5.438, P=0.020). Conclusions The newly reported HIV/AIDS cases at ages of 50 years and older were predominantly local male farmers in Zhengzhou City from 2017 to 2021, with sexual contact as the main transmission route and medical institutions as the predominant route of detection. The proportion of newly reported HIV/AIDS cases appeared a tendency towards a rise.

Objective To observe the effect of cerebral blood flow on Broca aphasia patient of cerebrovascular disease by early rehabilitative intervention, to evaluate the result of early rehabilitative intervention, to ques the marker evaluating early effect. Methods 41 Broca aphasia patients were divided to rehabilitation groups (23 patients) and control groups(18 patients) according to the sequence of hospitalization, and meanwhile 37 patients of health examination were chosen as health control groups, the patients of rehabilitation groups were treated with drug and rehabilitative intervention but the patients of control group were treated only with drug, the average cerebral blood flow of left middle cerebral artery of both groups and health control group was examined with transcranium doppler before and after treatment. Results The average cerebral blood flow of left middle cerebral artery of rehabilitation group and control group was not different before treatment, the rehabilitation group and control group was less than health control group; the average cerebral blood flow of left middle cerebral artery of rehabilitation group and control group was increased obviously after treatment than that before treatment; the average cerebral blood flow of left middle cerebral artery was not different between rehabilitation group and health control group after treatment, it was different between control group and health control group; the average cerebral blood flow of left middle cerebral artery was increased obviously on rehabilitation group than that on control group after treatment. Conclusions the early rehabilitative intervention can increase the average cerebral blood flow of left middle cerebral artery of Broca aphasia patient accelerate the functional restoration; TCD can be regarded as an early marker to evaluate early effect.

Objective To report 49 cases of adult interatrial septal defect with pulmonary hypertension operated and to evaluate their results.Method Most of those cases were repaired with conventional operative procedures,but 2 cases among them with severe pulmonary hypertension were repaired with one-way valvular patch.Those with mitral or tricuspid remarkable regurgitation,were repaired with annuloplastic procedure at the same time.Results There were no operative death,and followed up for one to five months,the heart size,ECG and the data of blood gas analysis were retuned into normal value.Heart function of 47 case was raised to Ⅰ class(NYHA),and 2 case to Ⅱ class.Conclusions Operation for adult interatrial septal defect especially with pulmonary hyperteasion is highly indicated.Treatment of mitral or tricuspid regurgitation remarkably at the same time is important for recovery of cardiac function.One-way valvular patch used to repair ASD with severe pulmonary hypertension could reduce complication and mortality,so it is an effective procedure adapted.

Objective: To construct a replicating adenovirus vector CNHK600-p53 carrying p53 gene and investigate its effect on the chemosensitivity of hepatocarcinoma cell line. Methods: Chemotherapy of hepatocarcinoma cel ls BEL-7404 were carried out using Fluorouracil, Mitomycin, Epirubicin, CNHK600-p53, CNHK600-p53 + Fluorouracil, CNHK600-p53 + Mitomycin, and CNHK600-p53 + Epirubicin, separately. MTT assay was used to evaluate the killing effects after therapy. Results: The inhibition rate on BEL-7404 cells was(47±3)% whe n using 100 μg/ml 5-Fu, was (20±4)% when using 1 μg/ml MMC, and was (73±2)% when using 2.5 μg/ml EPI. The inhibition rates of BEL-740 cells in CNHK600-p53 (MOI=10) + Fluorouracil (100 μg/ml), CNHK600-p53 (MOI=10) + Mitomycin (1 μg/ml), and CNHK600-p53 (MOI=10) + Epirubicin (2.5 μg/ml) groups were (59±4)%, (44±4)% and (86±2)%, respectively (P<0.01). Conclusion: Adenovirus CNHK600-p53 carrying p53 gene can enhance chemosensitivity of BEL7404 hepatocarcinoma cell line. Gene therapy using adenovirus CNHK600-p53 in combination with chemotherapy may be a new strategy for hepatocarcinoma treatment.

Objective: To compare the phenotype of induced dendritic cell (DC) derived from peripheral blood of the healthy individuals and the patients with oral carcinoma. Methods:15 patients with oral carcinomal at stages II or above were assigned to study group and 15 cases of healthy volunteers were assigned to control group. Leukocyte were isolated by density centrifugation. Adherent fractions were incubated in present of GM-CSF,IL-4 and TNF-? for 7 days in vitro. The morphological change of the cultured cells was observed by light microscopy and electronic microscopy. The phenotype expressions of CD83,CD1a,CD86, CD80, HLA-DR were analysized by flow cytometry. Results:Typical DCs were obtained from two groups after 7 days culture. The expression rate of CD83,CD1a on DC from healthy volunteers was significantly higher than that from patients (P0.05). Conclusion:DC derived from oral cancer patient' s peripheral blood monocyte might be more matured and is a good cell model for further immunotherapy and research.

This paper reported the estimation of free calcium concentration in rabbit resting lymphocytes, and the effect of (-) Daurisoline (8701) on the cyto-plasmic free calcium concentration by using fluorescent indicator Fura -2/Am estimating technique. Results were compared with the effect of the Ca2-ionophore A23187.The results indicated that the free calcium concentration of resting lymphocytes was 109. 6nmol ? L-1, after 10-5 mol? L-1,10-4.5 mol ? L-1,and 10-4 mol ? L-1-)Daurisoline administration, the cyto-plasmic free calcium concentration increased to 178. 8%, 204. 4%and 817. 5% respectively, while same concentration A23187 only led the increase of 164. 6% , 198.4% and 327% respectively.Results suggested that ( - )Daurisoline may be a Ca2- agonist and may induce the cytotoxicity effect on lymphocytes at high concentr ation.

Objective: The studies were carried out on the endogenous phytohormone changes of Panax notoginseng seeds during the after-ripening process in order to understand the machanism of endogenous phytohormones (GA, IAA, ZR, and ABA) in the process of embryo development and dormancy release. Methods: Dynamic changes of endogenous phytohormones of P. notoginseng seeds were measured by ELISA method through the entire after-ripening process. Results: The contents of endogenous GA, IAA, and ZR increased with the embryo development, while the ABA content decreased contrarily. Conclusion: The change of phytohormone balance is one of determining factors for relieving dormancy and promoting germination of P. notoginseng seeds.