1.Repairing maxillofacial and neck scar by overlapping tissue expansion of neck
Huabin ZHANG ; Jiyang CHEN ; Dongyuan SUN ; Hui MENG ; Shuangqing LI
Chinese Journal of Medical Aesthetics and Cosmetology 2009;15(5):301-303
Objective To summarize overlapping tissue expansion of neck without platysma in repairing maxillofacial and neck scar contracture deformity.Methods Two expanders were buried in the same soft tissue pocket superficially to the platysma in an overlapping pattern,water injection were on schedule,and secondary operation was performed after 4 to 6 weeks.Results 16 cases of maxillofacial and neck scar contracture deformities were treated with overlapping tissue expansion of neck without platysma since 2004.Good results were achieved except one case of expander exposure,but the final resuit was good after suitable treatment.Conclusion The overlapping tissue expansion technique can provide much more expanded tissue and reduce complications compared with the traditional expansion technique,especially using overlapping tissue expansion of neck without platysma for repairing the defects at the maxillofacial and cervical region.
2.Cosmetic repair for face scar deformity by small-capacity tissue expansion
Huabin ZHANG ; Jiyang CHEN ; Dongyuan SUN ; Hui MENG ; Shuangqing LI
Chinese Journal of Medical Aesthetics and Cosmetology 2001;0(04):-
Objective To investigate the clinical value of face scar deformity by small-capacity tissue expansion. Methods A small-capacity expander of 10~100 ml was implanted into the hypoderm, and then regular affusion was made with injection pot outside or inside. After expanding for four weeks to eight weeks, the expander was removed and the removing wound surface of scar was repaired with flap. Results After clinical application in 32 cases, there were complications such as infection and expander's exposure occurred in two cases, but the final result was good after suitable treatment. All cases were satisfied with unclear scar after 6 to 36 months’ follow-up. Conclusions Positive cosmetic effect can be received with small-capacity tissue expansion.
3.Parthenolide enhances the apoptosis induced by 4-hydroxyphenyl-retinamide in human hepatoma cells
Lan LIU ; Dongyuan XU ; Wanshan YANG ; Shu SUN
Chinese Journal of Pathophysiology 1999;0(09):-
AIM: To detect the signal pathway of apoptosis induced by 4-hydroxyphenyl-retinamide(4-HPR) and the biological effect of parthenolide-induced apoptosis.METHODS: TUNEL staining,FCM analysis,electrophoretic mobile shift assay(EMSA) were used to determine the actual effects and its mechanism of parthenolide on the 4-HPR-induced apoptosis in human hepatoma Hep-3B and SK-Hep-1 cells.RESULTS: The results of TUNEL and PI staining showed that parthenolide selectively enhanced 4-HPR-induced apoptosis in Hep-3B and SK-Hep-1 cells.Subsequent observations using EMSA assay indicated that parthenolide effectively inhibited NF-?B activation during fenretinide-induced apoptosis.CONCLUSION: These findings indicate that parthenolide suppresses 4-HPR-induced apoptosis via inhibition of NF-?B activation and that NF-?B activation during fenretinide-induced apoptosis might have an anti-apoptotic effect.
4.Improved lipid productivity of Nannochloropsis by heavy-ion irradiation mutagenesis.
Zhiyao WANG ; Yubin MA ; Runzhi MU ; Changjiang SUN ; Dongyuan ZHANG ; Yongfei WANG
Chinese Journal of Biotechnology 2013;29(1):119-122
Nannochloropsis has been considered as a promising feedstock for biodiesel production in recent years. To improve its lipid productivity, heavy-ion irradiation mutagenesis, an effectively breeding method used in plants and microorganisms was applied in Nannochloropsis oceanica OZ-1. After large-scale screening using Imaging-PAM and microplate-reader, two mutants (HP-1 and HP-2) with higher growth rate were isolated from the wild type N. oceanica. Subsequently analysis showed that after 18 days of cultivation biomass accumulation of the HP-1 and HP-2 mutant was increased by 18% and 26% respectively compare to the wild type. Total lipid productivity of the HP-1 and HP-2 mutant was 295 mg/(L x d) and 275 mg/(L x d), respectively, whereas that of the wild type was 247 mg/(L x d). Both mutants showed significantly advantage over their wild type concerning biomass accumulation and lipid productivity.
Heavy Ions
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Lipids
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biosynthesis
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Microalgae
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growth & development
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metabolism
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radiation effects
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Mutagenesis
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genetics
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Mutation
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genetics
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Stramenopiles
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genetics
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metabolism
;
radiation effects
5.Correlation between Genetic Variants and Polymorphism of Caveolin and Sudden Unexplained Death.
Fang Yu WU ; Xin Hua TANG ; Lian Lei GAI ; Xiao Ping KONG ; Bo HAO ; Er Wen HUANG ; He SHI ; Li Hui SHENG ; Li QUAN ; Shui Ping LIU ; Bin LUO
Journal of Forensic Medicine 2017;33(2):114-119
OBJECTIVES:
To explore the genetic variation sites of caveolin (CAV) and their correlation with sudden unexplained death (SUD).
METHODS:
The blood samples were collected from SUD group (71 cases), coronary artery disease (CAD) group (62 cases) and control group (60 cases), respectively. The genome DNA were extracted and sequencing was performed directly by amplifying gene coding region and exon-intron splicing region of CAV1 and CAV3 using PCR. The type of heritable variation of CVA was confirmed and statistical analysis was performed.
RESULTS:
A total of 4 variation sites that maybe significative were identified in SUD group, and two were newfound which were CAV1: c.45C>T (T15T) and CAV1:c.512G>A (R171H), and two were SNP loci which were CAV1:c.246C>T (rs35242077) and CAV3:c.99C>T (rs1008642) and had significant difference (P<0.05) in allele and genotype frequencies between SUD and control groups. Forementioned variation sites were not found in CAD group.
CONCLUSIONS
The variants of CAV1 and CAV3 may be correlated with a part of SUD group.
Caveolins/genetics*
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Coronary Artery Disease
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Death, Sudden/etiology*
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Exons
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Genotype
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Humans
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Male
;
Polymerase Chain Reaction
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Polymorphism, Single Nucleotide
6.Carcinoma-associated fibroblast-derived lysyl oxidase-rich extracellular vesicles mediate collagen crosslinking and promote epithelial-mesenchymal transition via p-FAK/p-paxillin/YAP signaling.
Xue LIU ; Jiao LI ; Xuesong YANG ; Xiaojie LI ; Jing KONG ; Dongyuan QI ; Fuyin ZHANG ; Bo SUN ; Yuehua LIU ; Tingjiao LIU
International Journal of Oral Science 2023;15(1):32-32
Carcinoma-associated fibroblasts (CAFs) are the main cellular components of the tumor microenvironment and promote cancer progression by modifying the extracellular matrix (ECM). The tumor-associated ECM is characterized by collagen crosslinking catalyzed by lysyl oxidase (LOX). Small extracellular vesicles (sEVs) mediate cell-cell communication. However, the interactions between sEVs and the ECM remain unclear. Here, we demonstrated that sEVs released from oral squamous cell carcinoma (OSCC)-derived CAFs induce collagen crosslinking, thereby promoting epithelial-mesenchymal transition (EMT). CAF sEVs preferably bound to the ECM rather than being taken up by fibroblasts and induced collagen crosslinking, and a LOX inhibitor or blocking antibody suppressed this effect. Active LOX (αLOX), but not the LOX precursor, was enriched in CAF sEVs and interacted with periostin, fibronectin, and bone morphogenetic protein-1 on the surface of sEVs. CAF sEV-associated integrin α2β1 mediated the binding of CAF sEVs to collagen I, and blocking integrin α2β1 inhibited collagen crosslinking by interfering with CAF sEV binding to collagen I. CAF sEV-induced collagen crosslinking promoted the EMT of OSCC through FAK/paxillin/YAP pathway. Taken together, these findings reveal a novel role of CAF sEVs in tumor ECM remodeling, suggesting a critical mechanism for CAF-induced EMT of cancer cells.
Humans
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Paxillin/metabolism*
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Protein-Lysine 6-Oxidase/metabolism*
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Carcinoma, Squamous Cell/pathology*
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Epithelial-Mesenchymal Transition
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Integrin alpha2beta1/metabolism*
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Mouth Neoplasms/pathology*
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Collagen/metabolism*
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Fibroblasts
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Extracellular Vesicles/metabolism*
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Cell Line, Tumor
;
Tumor Microenvironment