1.Effect of conventional ventilation and inhaled nitric oxide In Infants with persistent pulmonary hypertension of the newborn
Chinese Pediatric Emergency Medicine 2010;17(3):230-232
Objective To evaluate the effects of inhaled nitric oxide (iNO) therapy combined with conventional ventilation in the infants with persistent pulmonary hypertension of the newborn (PPHN).Methods NO inspiration was added for 22 infants with PPI-IN ventilated with FiO2≥0. 9,PIP≥30 cm H2O,PEEP≥3 cm H2O,RR≥50 bpm for 4~6 hours,with SpO2 still < 90% and PaO2 <55 mm Hg. The iNO concentration started at (10~20)×10-6 for 20 infants,and (20~40)×10-6 for 2 infants. The iNO would be stopped when the concentration reached 40×10 -6 without any sign of improvement. The SpO2 ,blood gas analyses,blood pressure, heart rate and NO: concentration were moraitored during therapy and the resulting data compared to readings before administration of int. Results Clinical situation were significantly improved in 20 (91%) of the infants with SpO2 gradually going up after 10 minutes of int. Before iNO,mean Fit2 was 0. 9±0. 1 ,SpO2 was(76. 3±13.3)% ,and Pat2 was (46. 4±10. 1 ) man Hg. From 1 to 6 hours after iNO,SpO2 increased to(95. 1±3.8)% ,Pat2 increased to(92. 8±24.7) mm Hg,FiO2 decreased to 0.6 s0. 1.The differences were significant (P < 0. 01 ). Eighteen of 22 (82%) infants surviving. Conclusion iNO is effective in alleviating PPHN in infants. There are no remarkable side effects. It is more beneficial to start the iNO concentration at (10~20)×10-6 while some infants may need NO concentration at up to (20~40)×10-6.
2.Clarithromycin in the Treatment of Upper Respiratory Tract Mycoplasmal Infections in Children
China Pharmacy 2001;0(11):-
OBJECTIVE:To evaluate the efficacy of clarithromycin in the treatment of upper respiratory tract mycoplasmal infections(URTI)in children METHODS:68 cases were divided into two groups according to different therapeutic schemes:observation group(38 patients)received clarithromycin,10~15mg/(kg?d),bid,po,double the first dose;control group(30 patients) were treated with erythromycin,20~30mg/(kg?d),tid,po or iv drip qd for 7 days RESULTS:Observation group had significantly higher overall effective rate(P
3.Rheological properties of the common carotid artery in young versus aged cadavers
Donghui XU ; Shuhua HUANG ; Xinying LI ; Dongyuan LI
Chinese Journal of Tissue Engineering Research 2017;21(16):2527-2533
BACKGROUND: With age and blood pressure increasing, the damaged elastic membrane induced by degenerative vascular matrix results in calcium and collagen deposition, and finally expanded large arteries and aortas are easy to cause arteriosclerosis. OBJECTIVE: To compare the rheological properties of the common carotid artery in the youth and elderly, thus providing a basis for the study on the pathogenesis of atherosclerosis of the common carotid artery. METHODS: Common carotid arteries were respectively removed from the young and elderly cadavers. Ten samples from each group were randomly selected, and subjected to stress relaxation and creep tests. Meanwhile, one sample from each group was selected for histological observation. RESULTS AND CONCLUSION: The stress at 7200 s in the young group was significantly decreased compared with the elderly group (P < 0.05). The strain at 7200 s in the young group was significantly increased compared with the elderly group (P < 0.05). Scanning electron microscope observed that in the elderly group, there were lipid plaques and foam cell accumulation; the internal elastic lamina and middle elastic plate presented with degeneration, rupture and disintegration; and there were abundant plaques and inflammatory cells in the intimal and media, which characterized as atherosclerosis. While no lipid plaques were found in the young group. These results indicate that the elastic fibers and collagen fibers in the common carotid artery arrange in disorder as age increases, and the rheological properties are also changed.
4.Changes in marrow fat content of non-dialysis chronic kidney disease patients assessed by T2 *-corrected water-fat MRI
Biao LIU ; Hanzhuan LUO ; Xueping LI ; Bo HUANG ; Dongyuan LIN
Journal of Practical Radiology 2017;33(6):599-602
Objective To detect the changes in vertebral marrow fat fraction (MFF) using T2 *-corrected water-fat MRI and to analyze the relationships between MFF with bone biomarkers of non-dialysis chronic kidney disease (CKD).Methods 78 CKD patients were divided into five groups according to the eGFR and underwent water/fat MRI to obtain MFF.The reliability of MFF measurements by two radiologists was assessed with intra-class correlation coefficient (ICC).Serum calcium,phosphorus,alkaline phosphatase,osteocalcin,intact parathyroid hormone and 25(OH)D3 were determined.Results Mean CV for MFF measurements reproducibility was 2.37%.The inter-observer agreement for MFF was excellent (ICC=0.901).The ICC for each intra-observer agreement was excellent (ICC=0.959 and 0.948,respectively).There were statistical differences in MFF among five groups of CKD.Changes of MFF were earlier than those of serum calcium,phosphorus,parathyroid hormone,alkaline phosphatase and osteocalcin.MFF was positively correlated with serum phosphorus (r =3.011,P =0.003),parathyroid hormone (r=3.852,P<0.001),and negatively associated with calcium (r=-2.767,P=0.017),25(OH)D3 (r=6.032,P<0.001),eGFR (r=-5.104,P<0.001),respectively.Multivariable regression analysis showed MFF was negatively correlated with 25(OH)D3(Sβ=-0.343)and eGFR(S(S=-0.284,P<0.001).Conclusion CKD patients had higher marrow fat.T2 *-corrected water fat MRI could serve as a useful tool to quantify marrow fat content for CKD patients.
5.Preparation of andrographolide-loaded solid dispersions
Dongyuan SHAN ; Ying PEI ; Qing MENG ; Tong LI
Chinese Traditional Patent Medicine 2017;39(4):719-723
AIM To prepare the andrographolide-loaded solid dispersions.METHODS Differential scanning calorimetry (DSC) and X-ray powder diffraction (XRPD) were used for the analysis of solid dispersions previously prepared from the mixture of andrographolide and PVPK30 (1 ∶ 9) in autoclave.In addition to the evaluation index of in vitro dissolution rate,the influencing factors of pressure,temperature and reaction time were taken into consideration for the preparation optimization by Box-Behnken method.RESULTS Andrographolide was totally dispersed in solid dispersions in an amorphous state,manifesting an inhibited crystal diffraction peaks' formulation.Under the optimal conditions of 21.26 MPa for pressure,40.76 ℃ for temperature,and 1.13 h for reaction time,the in vitro dissolution rate was 85.49%.CONCLUSION After andrographolide is made into solid dispersions,its in vitro dissolution rate is obviously increased.
6.Repairing maxillofacial and neck scar by overlapping tissue expansion of neck
Huabin ZHANG ; Jiyang CHEN ; Dongyuan SUN ; Hui MENG ; Shuangqing LI
Chinese Journal of Medical Aesthetics and Cosmetology 2009;15(5):301-303
Objective To summarize overlapping tissue expansion of neck without platysma in repairing maxillofacial and neck scar contracture deformity.Methods Two expanders were buried in the same soft tissue pocket superficially to the platysma in an overlapping pattern,water injection were on schedule,and secondary operation was performed after 4 to 6 weeks.Results 16 cases of maxillofacial and neck scar contracture deformities were treated with overlapping tissue expansion of neck without platysma since 2004.Good results were achieved except one case of expander exposure,but the final resuit was good after suitable treatment.Conclusion The overlapping tissue expansion technique can provide much more expanded tissue and reduce complications compared with the traditional expansion technique,especially using overlapping tissue expansion of neck without platysma for repairing the defects at the maxillofacial and cervical region.
7.GC-MS Analysis and Biological Activities in vitro of the Supercritical CO2 Extracts from the Roots of Poly-althia nemoralis
Dongyuan HUANG ; Jiawei LIU ; Wuguo LI ; Weijie LIN
China Pharmacy 2016;27(1):15-17
OBJECTIVE:To extract and identify the volatile components of the roots of Polyalthia nemoralis,and to evaluate its biological activity in vitro. METHODS:Supercritical CO2 extraction (SFE-CO2) was performed to extract volatile compounds from the roots of P. nemoralis,and the volatile components were separated and determined by GC-MS. Human hepatic cancer Huh-7 cells were cultured with 0(blank control),5,10,20,30,40 and 50 μg(medicinal material)/ml extract for 24 h,and then MTT assay was used to detect the inhibitory effect of the extracts on cells. Relative cell viability and IC50 were calculated. The an-ti-bacterial activities of extract to Staphylococcus aureus,Methicillin-resistant Staphylococcus aureus,Enterococcus faecalis and oth-er strain were determined by Kirby-bauer method and broth dilution method. RESULTS:Forty compounds were identified from the SFE-CO2 extracts from the roots of P. nemoralis. The main constituents were(Z,Z,Z)-9,12,15-octa-decatrien-1-ol,glycerin,cinna-maldehyde,n-hexadecanoic acid and eugenol. Compared with blank control group,SFE-CO2 extracts from the roots of P. nemoralis 5 μg(medicinal material)/ml and above showed significant inhibitory effect on cell growth(P<0.05),and the inhibitory effect was strengthened as the concentration of extracts increased,IC50 values was 5.2 μg(medicinal material)/ml. However,the supercritical extract didn't showed antibacterial activity against three microorganisms in 2 kinds of antibacterial tests. CONCLUSIONS:SFE-CO2 and GC-MS method can effectively extract and identify volatile components of the roots of P. nemoralis,and supercritical extracts can inhibit the viability of cells but have no antibacterial activity.
8.Cosmetic repair for face scar deformity by small-capacity tissue expansion
Huabin ZHANG ; Jiyang CHEN ; Dongyuan SUN ; Hui MENG ; Shuangqing LI
Chinese Journal of Medical Aesthetics and Cosmetology 2001;0(04):-
Objective To investigate the clinical value of face scar deformity by small-capacity tissue expansion. Methods A small-capacity expander of 10~100 ml was implanted into the hypoderm, and then regular affusion was made with injection pot outside or inside. After expanding for four weeks to eight weeks, the expander was removed and the removing wound surface of scar was repaired with flap. Results After clinical application in 32 cases, there were complications such as infection and expander's exposure occurred in two cases, but the final result was good after suitable treatment. All cases were satisfied with unclear scar after 6 to 36 months’ follow-up. Conclusions Positive cosmetic effect can be received with small-capacity tissue expansion.
9.Observation in effect of different follow-up types on discharged patients with indwelling D-J stents
Xiangfeng QUAN ; Ji JING ; Suqing LU ; Yuqin LI ; Xuelin WEN ; Dongyuan TANG ; Linling WU ; Ruilian WU
Chinese Journal of Practical Nursing 2011;27(5):17-19
Objective To explore the effect of health education through different postoperative follow-up method, including telephone、Email and QQ, on patients with indwelling D-J stents. Methods 319patients with indwelling D-J stents were divided into the control group(88 cases), the telephone group(89 cases), the Email group (70 cases) and the QQ group (72 cases). M1 patients received rourine health education during hospitalization and before discharge, the latter three groups received follow-up by telephone、Email and QQ after discharge respectively. The rehabilitation effect was observed in the four groups. Results The complication rates of the telephone group, the Email group and the QQ group was significantly less and the mastering of knowledge about prevention and handling of complication was better than the control group during the follow-up. Conclusions Different types of follow-up can be selected by patients according to their actual status. Decreasing complication rate, favorable social benefit and approval of the patients family members will be seen due to involvement of the patients family in health education.
10.Mechanism of Neural Stem and Progenitor Cells Mediated by Hypoxia Inducible Factor-1α in Ischemic Stroke Model (review)
Dongyuan ZHANG ; Ming LI ; Zhengze WANG ; Wenran ZHANG ; Xiaodan XU ; Liying ZHANG ; Shen TIAN
Chinese Journal of Rehabilitation Theory and Practice 2017;23(3):319-322
Hypoxia inducible factor-1αis a kind of hypoxia response factor. Acute cerebral ischemia and anoxia can induce up-regula-tion of hypoxia inducible factor-1α, and the downstream genes, which plays a role in both the energy metabolism and collateral circulation after cerebral ischemia. Neural stem and progenitor cells regeneration also benefit the functional outcome after ischemic stroke. Hypoxia in-ducible factor-1αmay induce proliferation and differentiation of neural stem and progenitor cells through Notch, Wnt/β-catenin pathways, etc., in the ischemic stroke model.