1.The growth-inhibitory effect of endometrial carcinoma cell line HEC-1A induced by somatostatin receptor family
Journal of Xi'an Jiaotong University(Medical Sciences) 1981;0(03):-
Objective To study the expression of somatostatin receptor family in endometrial carcinoma cell line HEC-1A, as well as the growth and apoptosis of HEC-1A cell line when it was treated with somatostatin analogue RC-160. Methods RT-PCR was used to examine the existence of somatostatin receptors on the HEC-1A cells. The anti-proliferative effect of RC-160 on the growth of HEC-1A cells was detected by using BrdU incorporation test. TUNEL staining was used to find out whether apoptosis was involved in the inhibitory process. Results All the five somatostatin receptor subtypes were expressed in HEC-1A cells. RC-160 inhibited the HEC-1A cell growth stimulated by serum in a dose-dependent manner. The effect was maximal at the concentration of 10 -5 mol/L after 48 hours' treatment. No apoptosis was detected. Conclusion Somatostatin analogue RC-160 can inhibit the proliferation of endometrial carcinoma cell line HEC-1A through binding to the somatostatin receptors on the cells. It seems that apoptosis is not the major cause of the inhibition.
2.The ancient clinical application of umbilical therapy for retention of urine.
Chinese Acupuncture & Moxibustion 2017;37(2):185-187
By collecting relevant ancient literature, with retention of urine, umbilical therapy and Shenque (CV 8) as key words, various umbilical therapies for retention of urine from thedynasty todynasty were retrieved and summarized. The results indicated rich knowledge of umbilical therapy was recorded in ancient literature (thedynasty to thedynasty), including 9 kinds of therapies e.g. sticking umbilical method, ironing umbilical method, pasting umbilical method, smearing umbilical method, filling umbilical method, sealing umbilical method, covering umbilical method, dripping umbilical method and bundling umbilical method. Moreover, the detailed methods were briefly explained. It is hoped to provide reference for modern application of umbilical therapy for retention of urine.
3.Determination of hesperidin and salvianolic acid B in Xiaopikang Capsule by HPLC
Junxiang WANG ; Dongying WANG ; Junyong LI
Chinese Traditional Patent Medicine 1992;0(09):-
AIM: To determine the contents of hesperidin and salvianolic acid B in Xiaopikang Capsule(Folium Citri reticulatae,Radix et Rhizoma Salviae miltiorrhizae,etc.) by HPLC. METHODS: The HPLC system consisted of Diamonsil C_(18) column(250 mm?4.6 mm,5 ?m), metanol-acetonitrile-formicacid-water(30∶10∶1∶59) mixture as mobile phase,detection wavelength at 284 nm,flow rate of 1.0 mL/min. RESULTS: The ca-(libration) curves of two ingredients were linear within the ranges of 0.22 ?g-2.2 ?g(r=0.999 6,hesperidin),(1.62 ?g-10.2 ?g)(r=0.999 9,salvianolic acid B),respectively.The average recoveries of hesperidin was(98.9%)(RSD=1.60%),salvianolic acid B for 99.02%(RSD=0.97%). CONCLUSION: The method is rapid,sensitive and accurate.
4.Immunoregulatory effect of Dachengqi decoction on endotoxic ARDS in rats
Yumei LI ; Hongchang WEI ; Dongying WANG
Chinese Journal of Pathophysiology 2000;0(10):-
AIM:To investigate the effect of Dachengqi decoction(DD)on the acute respiratory distress syndrome(ARDS)in rat model induced by endotoxin "two-hit".METHODS:48 healthy male Wistar rats were randomly divided into 4 groups:control group,ARDS model group,ARDS+DD treatment group and ARDS+dexamethasone treatment group(12 in each group).E.coli lipopolysaccharide(LPS)"two-hit" induced ARDS model in rats was established.The arterial blood gas analysis,lung wet/dry weight(W/D)ratio and lung tissue pathology observation and scoring were measured to evaluate the pharmacological effects of DD on ARDS.The levels of TNF-?,IL-1 and IL-10 were determined by ELISA to explore the immune regulatory mechanism of DD.RESULTS:(1)Treatment with DD significantly improved blood pressure in rats,increased oxygen saturation,decreased lung wet/dry weight ratio and lung injury score,relieved pulmonary edema and the inflammatory responses.(2)DD suppressed the productions of systemic and pulmonary pro-inflammatory mediators and promoted the release of anti-inflammatory mediators in lung.CONCLUSION:The inhibitory effects of DD treatment on pulmonary inflammatory response are not only related to reducing the extent of systemic inflammatory response,but also promote pulmonary anti-inflammatory production and regulate the balance of pulmonary pro-inflammatory mediators and anti-inflammatory cytokines.This specific regulatory effect protects the target organ from excessive inflammatory organ injury,discloses that DD has advantages in the treatment of endotoxic ARDS.
5.The Establishment and Application of Examination Question Database on Nutrition and Food Hygiene
Linjing WANG ; Shaoying FANG ; Dongying LIU
Chinese Journal of Medical Education Research 2003;0(04):-
The examination question database on Nutrition and Food Hygiene was set up and applied to reduce the effects of subjective factors on test efficacy.The test items of noun explanation,brief answer and discussion were compiled in the form of Microsoft Excel.Then the items were classified by Excel filtration function and a test paper was created by random sampling from the database.It reduces teachers' burden of making test questions and increases objectivity of test questions,which has achieved good results in teaching.
6.Therapeutic effectiveness of thalidomide to multiple myeloma and its mechanism.
Minglin WANG ; Yuefen LIU ; Yinggang LI ; Hongguang WU
Chinese Journal of Hematology 2002;23(10):514-516
OBJECTIVETo observe the effective mechanism and side effects of thalidomide to multiple myeloma (MM).
METHODSTen cases of MM were studied, of which 3 were previously untreated and 7 refractory or relapsed. Bone marrow microvascular density (MVD) was detected by factor-VIII related antigen and CD(34) immunohistological staining and serum concentration of vascular endothelial growth factor (VEGF) before and after treatment was determined by ELISA. The initial dosage of thalidomide was 100 approximately 200 mg/d with a weekly escalation of 50 mg/d to 450 approximately 650 mg/d. The therapeutic effectiveness is classified into partial remission, improvement and uneffective according to the decrease of serum M protein and bone marrow myeloma cells. Anemia, renal function and blood electrolytes were also observed.
RESULTSBefore treatment, MVD was 73.32 +/- 28.80 and 32.30 +/- 12.50 in MM and control group, respectively, (P < 0.01). MVD in MM group decreased to 56.12 +/- 19.34 after treatment, and was of significant difference (P < 0.05) as compared to the pretreatment value. However, there was still a significant difference as compared to control (56.12 +/- 19.34 vs 32.30 +/- 12.50, P < 0.01). The concentration of VEGF significantly decreased after treatment [from (178.23 +/- 26.56) ng/L to (78.48 +/- 19.98) ng/L, P < 0.01)]. The total effective rate was 70%. There were no serious side effects.
CONCLUSIONMVD and VEGF concentration were decreased obviously by thalidomide treatment. The dosage of 450 approximately 650 mg/d might be effective in refractory or initial MM.
Aged ; Angiogenesis Inhibitors ; adverse effects ; therapeutic use ; Antigens, CD34 ; analysis ; Bone Marrow ; blood supply ; drug effects ; Constipation ; chemically induced ; Endothelial Growth Factors ; blood ; Fatigue ; chemically induced ; Female ; Humans ; Immunohistochemistry ; Intercellular Signaling Peptides and Proteins ; blood ; Lymphokines ; blood ; drug effects ; Male ; Middle Aged ; Multiple Myeloma ; blood ; drug therapy ; pathology ; Nausea ; chemically induced ; Sleep Wake Disorders ; chemically induced ; Thalidomide ; adverse effects ; therapeutic use ; Treatment Outcome ; Vascular Endothelial Growth Factor A ; Vascular Endothelial Growth Factors ; von Willebrand Factor ; analysis
7.Estrogen-like effects of saikosaponin-d in mice.
Yong LI ; Peng WANG ; Jianlin REN ; Dongying YUAN ; Shouyi YIN
Journal of Integrative Medicine 2009;7(7):657-60
To investigate whether saikosaponin-d (SSd) had estrogen-like effects in mice.
8.Effects of periodontitis on the phenotype and secretion function of macrophages in C57 BL/6J mice model
Yixin XU ; Jincai ZHANG ; Dongying XUAN ; Ting YU ; Xiangli WANG
The Journal of Practical Medicine 2016;32(10):1568-1570
Objective To explore the splenic macrophages phenotype and secretory function of mice with periodontitis, so as to explore effects of periodontitis on macrophages. Methods 22 mice were randomly divided into periodental ligation group (group P10d ) and pseudo periodental ligation control group (group C), with 11 mice in each group. The experimental periodental ligation on mice lasted for 10 days before they were sacrificed. Flow cytometry was applied to detect the expression of M1 and M2 in mononuclear macrophages. Real-time PCR was applied to detect the relative expression of pro-inflammatory cytokines IL-1β and anti inflammatory cytokines IL-10. Results Compared with the control group C, the proportion of M1 macrophages in the periodontitis group decreased, and the ratio of M1/M2 was also decreased significantly, and IL-1β mRNA also down-regulated. Conclusions Chronic periodontal infection could down regulate the proportion of M1 macrophages , decrease ratio of M1/M2 and the expression of inflammatory cytokines IL-1βmRNA.
9.A Clinical Study of 46 Children with Cyclic Vomiting Syndrome
Guilian SUN ; Yajing JIANG ; Lin JIANG ; Dongying WANG ; Zhiliang YANG
Journal of China Medical University 2010;(1):67-70
Objective To analyze the clinical characteristics of children with cyclic vomiting syndrome (CVS), summarize the clinical experience, and improve the awareness,diagnosis and treatment level of CVS.Methods The clinical data and results of long-term follow-up of the children with CVS were collected and analyzed from 1995 to 2009 in our department.Results Forty-six children were enrolled in the study, including 22 boys and 24 girls.Mean onset age was 4.5 years(ranged from 1 to 11 years)and the mean age at final diagnosis was 8.5 years (ranged from 4.5 to 14.5 years).Sixty-six and seventy-four percent of patients had family history of migraine and motional sickness respectively,whereas 83% patients had triggers.The clinical manifestation of CVS was severe episodic vomiting.The episodes had a rapid onset and sudden ending,persisted for several hours to days,and were separated by symptom-free intervals.The incidental symptoms were pallor, lethargy,intractable nausea,abdominal pain,headache,photophobia and dizzy.Twenty-nine patients diagnosed were followed up for 5 years at average.Five patients were treated with valproate,4 with cyproheptadine, 5 with cyproheptadine and valproate,5 with amitriptyline,cyprohep tadine and valproate,and 3 with cyproheptadine and flunarizine.All treated patients recovered mean 10 months(1.5 months to 2 years) years later and displayed the reduced number of episodes or the severity of episodes except 3 patients.Twenty-eight percent (8/29) of patients progressed to migraine headaches.Conclusion CVS is a relatively common disease in children and awareness of the condition should be increased.CVS should be considered when patients had recurrent vomitting and were completely healthy between the two episodes after excluding other pathological conditions.
10.Expression and clinical significance of AOC4P in hepatocellular carcinoma
Qianqian HE ; Haolian WANG ; Jinping ZHANG ; Dongying LI ; Jiansheng LI
Chinese Journal of Clinical Oncology 2016;43(8):344-347
Objective:This study investigates the expression of long non-coding RNA termed amine oxidase, copper containing 4, pseudo-gene (AOC4P) in hepatocellular carcinoma (HCC) and its relationship with clinicopathological factors and patient prognosis. Methods:The expression of AOC4P in 106 HCC tissues and adjacent non-tumor tissues were detected using qRT-PCR. The relationship between AOC4P expression and clinicopathological factors, as well as patient prognosis, in HCC was analyzed. Results:The relative expression of AOC4P in the cancer tissues was 0.66±0.34, which was significantly lower than the relative expression in adjacent non-tumor tissues, i. e., 1.45±0.63 (P<0.05). Univariate analysis showed that low AOC4P expression was correlated with tumor size, lymph node metastasis, and TNM stage (P<0.05). The group with high AOC4P expression levels has longer survival time compared with the group with low AOC4P expression levels. Cox multivariate regression analysis showed that AOC4P expression, lymph node metastasis, and vascular in-vasion were all independent factors that affect prognosis of patients. Conclusion:AOC4P expression was reduced in HCC tissues, and AOC4P may play crucial roles in the occurrence and development of HCC. For patients with poor prognosis, low AOC4P expression is an independent risk factor for patients with HCC. Thus, AOC4P expression may be used as a molecular marker for HCC.