Objective: To study the action of acupuncture on the morbid psychology of the heroine addicts at late stage of detoxification. Methods: Four methods including acupuncture, combination of opium and acupuncture, combination of opium and buprenorphine, and combination of opium and Han's drug withdrawal instrument were adopted to study the effect of acupuncture on each factor in 90 listing symptoms of heroin addicts at late stage of detoxification by the self-evaluation scales. Result: Acupuncture had more advantages in improving obsessive symptoms, anxiety, psychogenic symptoms and No 10 factor (P＜ 0.01). Conclusion: Acupuncture can correct the morbid psychology of the addicts and help them enter the recovery stage smoothly.

OBJECTIVE: To compare the effects of de-addiction with the therapy of acupuncture, acupuncture plus opium, opium plus buprenorphine and opium plus Han's instrument for de-addiction and to study the effects of the four therapeutic methods on the protracted withdrawal syndrome and craving. METHODS: The effects of de-addiction were assessed with the opiate withdrawal scale and the craving degree with visual analogue scale (VAS). RESULTS: The dominance of acupuncture treatment for withdrawal syndrome appeared to be after the 6th day, and the dominance for controlling craving showed after the 8th day, moreover, there were little side effects. CONCLUSION: Acupuncture treatment had the potentiality of preventing relapse and could be used for treating the protracted withdrawal syndrome and psychic dependence during the period between the stages of abstinence and rehabilitation.

BACKGROUND:Primary culture in vitro of neurons plays an important role in the development, regeneration, signal transduction mechanisms, neuropharmacology and gene expressions of the nervous system.
OBJECTIVE:To establish a simple method for primary culture of high-purity cortical neurons in neonatal Sprague-Dawley rats.
METHODS:Cortical tissues were acquired from neonatal Sprague-Dawley rats born 1 day. In traditional experimental group, the whole cortex was removed;in improved experimental group, the cortical tissues, 2-3 mm thick on the brain surface were removed. Single cel suspensions were prepared after papain digestion and centrifugation and were then seeded onto 24-wel culture plates containing neuron solutions for primary culture (1×105 per wel ). Cel s were identified by neuronal specific markers MAP-2 and Tuj1 after 3-day culture. The number of neurons and neurite length were observed under inverted phase contrast microscope and recorded at 6, 24, 48 and 72 hours, 5 and 7 days of culture, resprctively.
RESULTS AND CONCLUSION:The cultured cel s expressing MAP-2 and Tuj1 were neurons that could be used in the fol owing experiments. The purity of neurons in the improved experimental group was 92%at 3 days, while only 51%in the traditional experimental group. Cel s in both two groups had attached to the wal presenting with smal processes at 6 hours, and a simple neural network formed at the 3rd day until dense neural networks could be found at the 5th day. To conclude, our culture method herein is simple and convenient, and can be used to produce neurons with high purity, which wil be helpful for the experimental studies on cortical neurons from Sprague-Dawley rats.

Objective To optimize preparation process of Zushima Gel Cream. Methods The comprehensive evaluation set sensory evaluation, initial adhesive force, viscous force, and peeling strength score as indexes. The mixing time, refining temperature, mixing speed, and powder adding sequence were investigation factors. Orthogonal experiment was used to optimize forming process. Results Conditions of optimized preparation process were as following: add Zushima powder in Viscomate NP-700 and glycerol; mixing time was 5 min; refining temperature was 40 ℃; mixing speed was 100 r/min. Conclusion The preparation process is good and optimized Zushima Gel Cream has a good adhesive force, good glossiness and excipients. The preparation process is good.

Objective:To investigate the effect of decoction of Fructus Mume (DFM) on the myoelectric activity of uterine smooth muscle of unpregnancy and early pregnancy rats and study its action mechanism.Methods: A pair of bipolar electrode was implanted on the serous surface of rat uterus. The DFM was injected into abdominal and the changes of uterine myoelectrical activity were observed.Results: The higher dose of DFM could enlarge the average amplitude of slow wave of uterine smooth muscle myoelectricity as well as increase the incidence and the maximal amplitude of the outbreak plexiform myoelectric wave. On the other hand, rats during early pregnancy were more sensitive to the DFM. Conclusions: The DFM enhances the myoelectricity activity of uterine smooth muscle of unpregnancy and early pregnancy rats which may result from enhacing the start cells electric activity and speeding up the action potential depolarization. Thus the DFM can serve as an effective clinical drug for anti early pregnancy.

OBJECTIVE: To assess the accuracy of a wrist worn Watch PAT 200 in diagnosing obstructive sleep apnea hypopnea syndrome (OSAHS) by comparing with the standard polysomnography (PSG). METHOD: Twenty-eight adults with suspected OSAHS underwent a standard in-lab PSG while wearing a Watch PAT 200. PSG events were manually scored according to standard criteria (AASM). Watch PAT data were collected including changes of apnea hypopnea, sleep stages, peripheral arterial volume, signal oxygen saturation and heart rate, and then apnea hypopnea index (AHI) was analyzed by an automatic algorithm. RESULT: For PSG, the mean score of AHI was (23.00 +/- 21.55)/h, and for Watch PAT, a mean score of AHI was (25.99 +/- 19.09)/h. There is a statistically positive correlation between PSG-AHI and PAT-AHI (r = 0.92, P < 0.01). The Coincidence rate of the sleep-wake assessment based on 30-second bins between the PSG and Watch PAT 200 was (89 +/- 6)%. CONCLUSION Watch PAT 200 could detected OSAHS based on AHI with comparable accuracy with standard PSG. And it would provide a reasonably accurate estimation of sleep and wakefulness stages in patients with OSAHS.
Adult ; Aged ; Aged, 80 and over ; Female ; Humans ; Male ; Middle Aged ; Monitoring, Ambulatory ; instrumentation ; Polysomnography ; instrumentation ; Sleep Apnea, Obstructive ; diagnosis ; physiopathology ; Sleep Stages

Objective To investigate the value of the immunohistochemistry and Western blot in the diagnosis of the benign muscular dystrophy with abnormal dystrophin expression.Methods The medical histories and clinical manifestations of 4 patients were collected.In addition to routine histological and histochemical studies,expression of dystrophin in muscle fibets was observed by immunohistochemical reaction(dys-N,dys-R and dys-C)and Western blot to anti-dystrophin antibody.Results Two patients had muscular weakness while another 2 patients had only muscular pain and elevated creatine kinase blood levels without muscular weakness.Histochemical stains showed atrophy,hypertrophy and fiber splitting in 2 patients,while only variation in fiber size was presented in anothor 2 patients.One patient had no reaction for dys-N,but had immunostains for dys-C and dys-R in the sarcolemma of muscle fibers.Western blot confirmed that the band of dys-C and dys-R was partly deficient,and the band of dys-N was absent compared with control.Three patients had no reaction for dys-R,but had immunostains for dys-C and dys-N.Compared with control,Western blot confirmed that the band of dys-R was absent,and the band of dys-C and dys-N were truncated.Conclusion The immunohistochemistry is stained with three anti-dystrophin antibodies to avoid diagnostic errors.Western blot is essential to further determine the type of dystrophin protein.

Objective:To explore the effects of dexmedetomidine (Dex) on perioperative intrapulmonary shunt rate (Qs/Qt), inflammatory factors and Claudin-4 in patients with esophageal cancer undergoing radical operation.Methods:Sixty patients with thoraco-laparoscopic combined esophageal cancer radical resection under general anesthesia were selected from the Second Hospital of Shanxi Medical University from March to August 2018. The patients were divided into Dex group (observation group) and 0.9% sodium chloride injection group (control group) according to the random number table method, and both groups were given the same anesthesia. In the observation group, Dex was injected intravenously before the anesthesia induction, the infusion was first performed at the loading dose of 1.0 μg/kg (the infusion was completed in 10 minutes) and then the infusion was performed at the rate of 0.4 μg·kg -1·h -1 until 30 minutes before the end of the operation. The control group was injected with the same dose of 0.9% sodium chloride injection. The radial artery blood and the subclavian venous blood was collected from the two groups at four time points of double lung ventilation for 15 minutes (T 0), 30 minutes (T 1) and 1 hour (T 2) after one-lung ventilation and 30 minutes (T 3) after the restoration of bipulmonary ventilation. The blood gas was analyzed, and Qs/Qt was calculated. The blood samples from subclavian vein were collected, and the serum levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and Claudin-4 were measured by enzyme-linked immunosorbent assay. Results:The Qs/Qt in the control group at T 0, T 1, T 2 and T 3 were (13.6±3.6)%, (36.1±2.9)%, (31.8±2.4)%, and (15.3±3.2)%, the difference was statistically significant, ( F = 397.273, P < 0.01), and the Qs/Qt in the observation group were (12.5±1.8)%, (27.4±3.0)%, (27.7±4.2)%, and (13.9±3.4)%, the difference was statistically significant, ( F = 205.124, P < 0.01); the Qs/Qt in the observation group at T 1 and T 2 were significantly lower than those in the control group ( t values were 178.011 and 23.791, both P < 0.05). The concentrations of TNF-α in the control group at T 0, T 1, T 2 and T 3 were (12.4±2.4) pg/ml, (20.5±3.0) pg/ml, (24.8±4.1) pg/ml, and (34.3±8.0) pg/ml, the difference was statistically significant, ( F = 109.749, P < 0.01), and the concentrations of TNF-α in the observation group were (11.4±3.0) pg/ml, (17.6±2.8) pg/ml, (17.4±3.2) pg/ml, and (26.2±5.0) pg/ml, the difference was statistically significant, ( F = 87.653, P < 0.01); the concentrations of TNF-α in the observation group at T 1, T 2 and T 3 were significantly lower than those in the control group ( t values were 10.471, 44.730 and 24.132, all P < 0.05). The concentrations of IL-6 in the control group at T 0, T 1, T 2 and T 3 were (18.4±4.0) pg/ml, (28.5±5.4) pg/ml, (40.1±6.0) pg/ml, and (43.1±6.0) pg/ml, the difference was statistically significant, ( F = 200.151, P < 0.01), and the concentrations of IL-6 in the observation group were (17.7±4.8) pg/ml, (21.9±3.9) pg/ml, (24.8±4.6) pg/ml, and (24.0±3.8) pg/ml ( F = 14.655, P < 0.01); the concentrations of IL-6 in the observation group at T 1, T 2 and T 3 were significantly lower than those in the control group ( t values were 38.983, 120.110 and 594.878, all P < 0.01). The concentrations of Claudin-4 in the control group at T 0, T 1, T 2 and T 3 were (5.9±0.8) ng/ml, (13.6±1.8) ng/ml, (14.7±4.5) ng/ml, and (16.8±2.5) ng/ml, the difference was statistically significant, ( F = 89.332, P < 0.01), the concentrations of Claudin-4 in the observation group were (5.5±0.7) ng/ml, (16.8±1.8) ng/ml, (18.0±4.8) ng/ml, and (21.2±4.4) ng/ml, the difference was statistically significant, ( F = 120.367, P < 0.01), the concentrations of Claudin-4 in the observation group at T 1, T 2 and T 3 were significantly higher than those in the control group ( t values were 54.619, 7.112 and 18.766, all P < 0.05). Conclusion:Dex can improve the intrapulmonary shunt to some extent, inhibit the inflammatory response during the operation, and increase the level of Claudin-4, which plays an active role in perioperative lung protection.

Objective: To study the biological effects of the HPV16Z-Hsp65-E6/E7 fusion protein vaccine on the tumor associated with HPV16 infection. Methods: We tested the cellular immune responsive intensity to the vaccine by the lymphocyte proliferation and CTL response, and studied the therapeutic effect of the vaccine on mouse TC-1 cell transplanted cancer in vivo and the influence on mouse lifetime. Results: The spleen lymphocytes from the C57BL/6 mouse immunized by the Hsp65-E6/E7 vaccine could proliferate obviously in the presence of the protein and TC-1 tumor cell could be lysed specifically by immune activated lymphocytes in vitro. This animal therapeutic experiment in vivo showed that the vaccine suppressed the growth of TC-1 cell transplanted tumor remarkably. Conclusion: The recombined vaccine can induce specific cellular immune response in vitro and suppress HPV16 positive TC-1 tumor cell growth obviously in vivo.

Objectives To analyze the characteristics of antigenic genes of clinical Bordetella pertussis strains recently isolated by analyzing the sequence of pertussis toxin S1 subunit(ptxS1) , pertactin (Prn) , fimbriae 2 (Fim2) and fimbriae 3 (Fim3 ) genes of four clinical isolates. Methods The 4 clinical isolates were collected in 2002 in Shijiazhuang of Hebei province. Four strains were isolated from pertussis patient's nasopharyngeal aspirate. ptxS1, Prn, Fim2 and Fim3 genes of these strains were amplified and sequenced. The sequences of those genes were compared with those of the isolates in GenBank and the isoaltes used in the production of pertussis vaccine in China. Results The results of the gene sequencing showed the four clinical isolates belonged to ptxS1 A type, which were different from those in vaccine strains. In addition, three Prn and three Fim'3 variants were observed in the four clinical isolates. Sequence analysis showed that the nucleotide sequence and deduced amino acid sequence of those strains had more than 99% identity with those in vaccine strains. The phylogenetic trees of those genes also showed these strains had a higher level of similarity with other Bordetella pertussis strains. Conclusion The four clinical isolates are different from vaccine strains in four antigenic genes, which laid a foundation for further studies on pertussis epidemiology,quality control and development of pertussis vaccine in China.