BACKGROUND:Bonemarrow mesenchymal stem cel transplantation can effectively improve decreased cardiac function caused by heart failure, but there is a lack of research about the effectinbone marrow mesenchymal stem cel transplantation on cardiac functioninheart failure induced by cardiomyopathies.
OBJECTIVE:To explore the effect of bone marrow mesenchymal stem cel transplantation on cardiac function in patients with cardiomyopathiesaccompanied by heart failure.
METHODS:Totaly 40 Sprague-Dawley rats were enroled, and bone marrow mesenchymal stem cels were isolatedfrom 10rats, and the remaining rats were equivalently randomized into normal, model and stem cel transplantation groups. Then rats in the model and stem cel transplantation groups were given intraperitoneal injection of hydrochloric acid doxorubicin to preparecardiomyopathy-inducedheart failure models. At 7 days after modeling, the stem cel transplantation group was treated with bone marrow mesenchymal stem celsthrough intravenous transplantation, and the model group was treated with equal amount of DMEM medium. Four weeks later, cardiac function of each rat was detected, and the cel survival and differentiation were observed by immunofluorescence method.
RESULTS AND CONCLUSION:At 4 weeks after transplantation, compared with normal and stem cel transplantation groups, the left ventricular systolic pressure and maximumrise/fal rate of left ventricular pressurewere significantly decreased, buttheleft ventricular end diastolic pressure significantly increased in the model group (P< 0.05). And there was no significant difference between the normal group and the stem cel transplantation group (P> 0.05).Highand dense fluorescence intensity was observed in the host myocardiumimmediatelyafter transplantation.Subsequently, the fluorescence intensityand density decreasedat 4 weeks, but the cel migration could be found, and some cels expressed cardiac troponin T. These results show thatintravenous transplantation ofbone marrow mesenchymal stem cels can improve cardiac function in rats withheart failure due tocardiomyopathies. Besides, the transplanted cels can survive in the host, and differentiate into cardiomyocyte-like cels.

Objective To compare the different effects of different titanic parameters on the expression of long-term potentiation (LTP) and to determine the optimal patterns of tetanizing stimulation in hippocampal CA1 area in adult male rats. Methods Four tetanic parameters of 100Hz were used in the experiment. Tetanus 1 was a short burst of stimulus composed of 60 pulses, which were divided into 5 sets, each set including 4-pulses-bursts. The inter-burst intervals are lengthened gradually, 1s, 5s, 30s, 10min in sequence. Tetanus 2 was a long burst of stimulus composed of 300 pulses in all, each burst including 20 pulses. The inter-burst interval is 2s, and tetanus 3 is also a short burst of stimulus composed of 15 bursts, each burst including 4 pulses. The inter-burst interval is 2s and tetanus 4 is a single train of burst composed of 60 pulses. The field excitatory post-synaptic potentials (fEPSPs) in hippocampal CA1 area were evoked by stimulating the Schaffer collateral fibers in the stratum radiatum and the expressions of LTP induced by the four parameters were evaluated. Results The amplitude and incidence of LTP induced by tetanus 1 were higher than those induced by the other three parameters. There was no statistical significance between the responses induced by the other three parameters. Both tetanus 1 and tetanus 3 could lead to long-lasting-LTP (LL-LTP, over 3h). Conclusion A high frequency stimulus with 200ms-inter-burst interval (theta frequency) is better in the induction of LTP in hippocampal CA1 area. A short burst of stimulus with 100Hz can evoke the expression of LL-LTP in hippocampal CA1 area.

Objective: To study the influence on the displacement of abutment with various retainer designs for bilateral distal-extension removable partial dentures(RPDs). Methods: Combined clasp, extension clasp and telescopic crown were used to retain RPDs. When food chip was masticated between the upper and lower dentition on one side, the displacement of abutment on both sides was measured by a high sensitive laser traneferring-detector and the data were statistically analyzed. Results: Inclination, torsion and vertical translocation of all abutment teeth were observed. Significant statistical difference ( P

Objective To sum up the problems should be taking care in operations for huge goitres. Methods Retrospectively analyzed the clinical data of 295 cases of huge goitres was made. Results All of the 295 cases had some degree of compress, replacement and bend of trachea by the goitre comfirmed by X ray examination .Among the 295 cases,162(54.9%) had trachea constriction.51(17.3%) had obvious dyspnea. Retrosternal goitres was found in 21 cases.12 complicated with secondary hyperthyroidism. All the 295 cases underwent subtotal or total thyroidectomy. Postoperative pathological diagnosis: nodular goitres was diagnosed in all the 259 cases (100%),including 11 cases(3.7%) with malignancy, 12 cases( 4.1% ) with secondary hyperthyroidism,and 39(15.1%) with adenomas. Conclusions Operation of huge goitres should choice appropriate anaesthetic method.For the main vessels of thyroid, very high position of the upper pole of thyroid, retrosternal goitres, malignancy of goitres etc,appropriate operative measures shoudd be adopted to prevent introoperative massive bleeding and damage of recurrent laryngeal nerve. If the indications of tracheostomy presents,a tracheostomy must be done.

Objective To improve the diagnosis and treatment of sliding hernia of adult urinary bladder. Methods Clinical data of 32 cases of adult sliding hernia of the bladder from 1984 to 2009 were analyzed retrospectively. Discomfort was complained in all the 32 patients along with palpable mass. Twentynine cases suffered from urgency of micturition, ascheturesis and dysuria, 15 cases had interruption of urination. The mass was saddle-shaped in 29 cases, when the bladder was filling. In all the 32 cases of hernia block were in a sense of fluctuations and flat on percussion. In 26 cases the bladder appeared a dumbbell-shaped image on cystography. By B-ultrasonic examination an opaque dark area of fluid was detected in 29 cases when there was retention of urine. Results Preoperatively 29 cases were diagnosed of bladder sliding hernia, 3 cases with misdiagnosis ( misdiagnosis rate of 9.4% ). 32 cases were cured by surgical treatment. The prolapsed portion of the bladder including stones in 2 cases were resected. 32 cases were followed up from 1 to 5 years without recurrence. Conclusions Once the sliding hernia of the bladder is diagnosed, patient should receive surgery. Intraoperatively the key of preventing inadvertent cut or missed diagnosis is familiar with pathological anatomy and careful exploration.

Objective To study the effects of gamma-aminobutyric acid (GABA) on the induction of long-term potentiation (LTP) in developing rat visual cortical slices. Methods Field potentials recorded from layer Ⅱ/Ⅲ induced by layer Ⅳ stimulation in rat visual cortical slices were examined. LTP was induced by tetanus in the slices on the postnatal 2, 3 and 5-week rats. The LTP incidences and the effects of GABA on them in different developmental stages were compared. Results LTP incidence at postnatal week 5 was significantly lower than that in other two groups. GABA A receptor antagonist picrotoxin could reverse this tendency, but had no effects on the LTP induction at the early postnatal age. Conclusion In visual cortex, GABA has inhibitory effects on the LTP induction only after the end of critical period and has no any facilitatory effects on the LTP induction before the developmental critical period.

Objective:To summarize diagnosis and surgical treatment of syndrome of splenic flexure of colon(SSFC) .Methods:The clinical data of SSFC in 81 patients between Jan,1987 and Dec,2008 were analysed retrospectively.Results:The main clinical manifestations were repeated abdominal pain,abdominal distension,constipation and chronic incomplete colon obstruction.X-ray barium enema showed that splenic flexure of colon was 7 cm higher than hepatic flexure of colon.The angle of splenic flexure of colon was less than 45 ?.Two patients underwent release of splenic flexure of colon and 3 patients accepted side-to-side anastomosis of splenic flexure of colon which were suitable to old,weak and emergent patients.The effects of these methods were well shorthy after operation,but not in long term(6 months to 2 years) .As to the rest 76 patients,thorough operation were done:subtotal resection of proximal segment colon(14 patients) ,subtotal resection of colon(20 patients) and total resection of colon(42 patients) .Conclusion:The diagnosis of SSFC is depended on clinical manifestations and barium enema.Thorough surgical treatment is the best method including proximal part resection,subtotal resection and total resection of colon.

Objective To study the changes of field potentials (FPs) during development and after long-term potentiation (LTP) induction in rat visual cortical slices. Methods Field potentials recorded from layer Ⅱ/Ⅲ induced by layer Ⅳ in the rat visual cortical slices were examined. The developing changes of FPs from postnatal days 8-13,15-21 and 29-35 were observed. Amplitudes, peak latencies and slopes of FPs before and after LTP induction were compared. Results Field potentials changed with the development, and the stimulating threshold at p15-21 was lower than that of the other two groups. The increase of FP amplitude was positively correlated with increase of its slope. The shortening of FP latency was positively correlated with the difference between changes of slope and amplitude. Conclusion Developing changes of field potentials reflect the development of visual cortex. The changes of FP slope follow the changes of its amplitude and latency, and thereby the slope is a proper parameter used as an index to evaluate LTP in visual cortex.

Objective To discuss the clinical effect of transurethral cystitis glandularis electrocision combined with treating the diseases under bladder outlet in female.Methods One hundred and two female cases of cystitis glandularis who received transurethral electrical resection of the bladder mucosa combined with treating the diseases under bladder outlet were selected as experimental group,and another 78 female cases were selected as control group,who only received transurethral electrical resection of the bladder mucosa.The visual analogue score (VAS) was observed before and after a 12-week treatment,and the efficacy was evaluated by analysis of non parameter RIDIT.Results The operation time in experimental group and control group were 15-40 (26 ± 13) min and 8-19 (13 ± 5) min,respectively,and there was significant difference between two groups (P ＜ 0.05).Two groups cases urinated smoothly after the removal of catheter,without the occurrences of urinary fistula and incontinence.During the 12-weeks of follow-up,44 cases were cured,31 cases improved,15 cases were effective and 12 cases unimproved in experimental group; 26 cases were cured,21 cases improved,16 cases were effective and 15 cases unimproved in control group.There was significant difference between two groups (R =0.576,95％ CI0.519-0.633,P＜ 0.05).Conclusion Although the operation time is lengthened by transurethral cystitis glandularis electrocision combined with treating the diseases under bladder outlet in female,this method obviously improves curative effect.

Objective: To investigate the effect of bromodomain-containing protein 4 (BRD4) gene on the apoptosis of cardiomy