Objective To explore the efficacy of double fork plate in treating the posterior wall fracture of the acetabulum.Methods Reviewed 11 patients who had treated with open reduction and internal fixation with double fork plates for posterior wall fracture of the acetabulum from July 2008 to July 2012,including 8 male cases and 3 female cases with an average age of 35.3 years(from 22 to 53 years).The average time from getting injury to operation was 7.8 days(from 1 to 19 days).The operations were performed through the Kocher-langenbeck approach.Double fork plates(formed from a three-or four-tubular plate) with an overlapping reconstruction plate used for posterior wall fragments.The functional outcomes were evaluated by the modified Merled'Aubigne and Postel'clinical garding system.The radiographs were graded according to the Matte criteria.Rmults The patients were adopted telephone or outpatient follow-up for 25 months on average(from 12 to 33 months).By the Matte criteria,7 cases achieved excellent reduction,3 good reduction,1 case fair reduction and none poor reduction,with the good and excellent rate of 90.9％.By the modified Merled'Aubigne and Postel'critria,functional recovery of the affected hip at the last followe-up was rated as excellent in 6 cases,good in 3 cases,fair in 2 cases and none poor,with the good and excellent rate of 81.8％.Heterotopic ossification was happened to 1 patient.Neither infection nor ischemic necrosis of the femoral head was recorded.Coclusion Double fork plates with an overlapping reconstruction plate can provid stable fixation and allow for early functional exercise.So the use of Double fork plates have a certain clinical value.

Objective To investigate the characteristics and the operative treatment effect of pelvic fractures complicated with lumbosacral plexus compression injury. Methods From January 2000 to January 2009, 19 patients (13 males, 6 females; mean age 34.7 years) of pelvic fractures complicated with lum-bosaeral plexus compression injury were treated by surgical fixation and neural decompression. The diagnosis of nerve compression were determined by analyzing the clinical manifestation and X-ray and CT imaging findings. The injured nerve were decompressed directly by remove the fracture fragments or callus which compressed the lumbersacralis plexus nerve. Eight cases of nerve decompression were through posterior ap-proach, six through anterior approach, and five through combined approach. Sixteen cases of unstable pelvis fractures were treated by open reducton and internal fixation. Results Seventeen patients were followed up. The mean follow-up time was 27 months (12-72 months). The fractures were clinically healed at 10.6 (8-14) weeks. Regarding the Neural Function of the lower extremity, 12 cases had achieved full recovery, 4 cases had recovered partly, 1 case had no change. Four of the six patients who have bladder and bowel dysfunc-tion had fully recovered while the other two patients still have urinary dysfunction. Conclusion Early and accurate diagnosis of the lumbosacral plexus compression injury accompanied with pelvic fractures were very important. It was key to better outcomes that early decompression the lumbosacral plexus and surgical fixa-tion the pelvis fractures are carried out after injury.

Objective To observe the effect of ammonia silver nitrate on early caries.Methods 100 cases of children with early caries treated in our hospital from April 2015 to December 2016 were admitted to conduct research and analysis.The children were divided into the control group and the observation group by double blind,and random method 50 cases in each group.The control group was treated with caries removal,the observation group was treated with carious worn out joint ammonia silver nitrate.After treatment, the two groups of children suffering from dental pulp tissue TNF-a, IL-8 concentration, the recurrence rate, filling rate falling, the rate of suffering from pulpitis, pain conditions were analyzed.Results After treatment, the observation group with TNF-α, IL-8 concentrations were lower than the control group(t=21.961,t=23.836), the difference was statistically significant (P<0.05);The recurrence rate, suffering from pulpitis rate was significantly lower than the control group, and the difference was statistically significant (P<0.05);the observation group after treatment in children with severe pain and painless was significantly lower than the control group, and the difference was statistically significant (P<0.05).Conclusion Ammonia silver nitrate is an effective and safe treatment for early caries, which can relieve the pain and discomfort in children, it is a safe and effective method of treatment and should be promoted and used in the clinic practice.

0.05). Conclusion The recombinant plasmid containing hBMP- 2 cDNA can be transfected and expressed effectively in rabbit MSCs

Objective To discuss the timing and methods of operative treatment of acetabular fractures of AO Type C (double- column fractures). Methods From February, 1999 to January, 2004, we treated operatively 46 cases of AO Type C acetabular fractures, including 23 cases of C- 1 type, 14 cases of C- 2 type, and 9 cases of C- 3 type. 15 cases were treated through ilioinguinal approach, 10 cases through improved Kocher- Langenbeck approach, and 21 cases through combined anterior and posterior approaches. There were 3 groups of patients according to the time from injury to operation: 15 cases got operation within 1st week after injury (Group A); 23 cases within 2nd week (Group B); 8 cases within/after 3rd week (Group C). All of them were checked with radiography and 3D CT scan before and after operation. Results 34 cases( 73.9% ) obtained anatomical reduction, 8 cases( 17.4% ) got imperfect reduction, and 4 cases( 8.7% ) had unsatisfactory reduction. All the patients were followed up for 1 year to 4 years, averaging 31 months. According to D' Aubigne and Postel Hip Score, joint function was rated as excellent in 32 cases( 69.6% ) , good in 5 cases( 10.9% ) , fair in 5 cases ( 10.9% ) and poor in 4 cases ( 8.7% ) . As far as reduction was concerned, there was a significant difference (P0.05). As far as joint function was concerned, there was a significant amelioration in Groups A and B compared with Group C (P0.05) between group A and Group B. Conclusions Good knowledge of AO classification of acetabular fractures combined with particular radiological data can result in correct diagnosis of Type C acetabular fractures. Operations should be carried out within 2 weeks after injury and efforts should be made to ensure anatomical reduction. To achieve good clinical results in treatment of Type C acetabular fractures, suitable approaches and correct reduction and internal methods are essential

Objective To investigate the drug-resistant status of Acinetobacter baumannii in clinical strains isolated from our hospital,and get to know the distribution of integrons carried by Acinetobacter baumannii,analyze the correlation between integron and drug-resistant of Acinetobacter baumannii..Methods Collected 85 Acinetobacter baumannii isolated in our lab,the drug-sensitivity tests were completed by the method of KB,the integrase genes were amplified by polymerase chain reaction(PCR),and identified the type of integron..Analyzed the correlation between the drug-resistant and integron in Acinetobacter baumannii..The open reading frame of integron were amplified by PCR,the polymorphism of integron was identified,and sequenced the PCR products.Results Except for Imipenem and Cefoperazone/ sulbactam(drug resistant rate less than 10%),the drug resistant rate of other 15 antibacterial was no more than 30% among the 85 Acinetobacter baumannii isolated in our lab..66.1 %(57/85) stains carried the integrons I,we didn't find out the integron Ⅱ and Ⅲ.The drug-resistant rates of Acinetobacter baumannii with integron were higher than that of Acinetobacter baumannii without integron.The length of amplified products in variable region of open reading frame of integron varied from 03 to 25kb,.The sequencing results confirmed the integron included multidrug resistant gene code.Conclusion In our hospital,the drug-resistant rate of Acinetobacter baumannii was high,and the majority were multidrug resistant strains.The drug-resistant rate of Acinetobacter baumannii was enhanced with integron,The multidrug resistant of Acinetobacter baumannii was associated with integrons.

Objective To study the genetic basis for biovar conversion of Y. pestis. Methods In silico comparative genomic analysis was conducted and some critical genetic variations of Yersinia pestis were comparatively analyzed by means of PCR and DNA sequencing. Results A 93bp in-frame deletion in glpD gene results in the glycerol negative characteristic of Orientalis strains. A point mutation in the napA gene may cause the negative characteristic of nitrate reduction in Mediaevalis and Microus strains. A 122-bp frameshift deletion in the araC gene may lead to the arabinose negative phenotype of Microus strains. Conclusion In this study, Microtus strains with their unique pathogenic, biochemical and molecular features, were proposed as a novel biovar Microtus. In the light of its differential ability to ferment glycerol and arabinose and to reduce nitrate, Y. pestis can be classified into four biovars-Antiqua(glycerol positive, arabinose positive and nitrate positive), Mediaevalis(glycerol positive, arabinose positive and nitrate negative), Orientalis(glycerol negative, arabinose positive and nitrate positive), and Microtus(glycerol positive, arabinose negative and nitrate negative).

Objective To study the genome dynamics of Y. pestis and look for the relationship between its genome microevolution and niche adaption.Methods The DNA microarray combined with PCR was used to perform comparative genomic analysis of natural populations of Y.pestis.Results It was revealed that considerable genome dynamics of Y. pestis were the result of gene acquisition and loss in genome. We established a genomotyping system to group homologous isolates of Y. pestis, drew an outline of parallel microevolution of the Y. pestis genome, and established the link between the bacterial niche adaptation and genome microevolution.Conclusion The transmission, colonization and expansion of Y. pestis in natural foci are the results of its parallel, directional and gradual adaptation to the complex interactions among the environment, the hosts, and the pathogen itself.

Objective To develop a whole-genome DNA microarray based on the genomic sequences of Y. pestis CO92 and 91001 and its use in comparative genomic analysis. Methods A total number of 4 005 genes of Y. pestis were amplified by PCR and printed onto glass slides in duplicate. Fluorescently labeled probes were prepared by marking genomic DNAs with random hexamers and Klenow. Labeled DNAs were hybridized with the microarrays by the method of two-fluorescence comparative hybridization. Three sets of two-fluorescence hybridizations were performed to examine the absence/presence of each gene. Results The results agreed with those derived from the in silico genomic comparison. Conclusion The results demonstrate that the microarry can be a useful tool for comparative genomic analysis of Y. pestis.

Objective To identify and compare the genome differences among live plague vaccines prepared with different strains of the bacillus. Methods The whole-genome DNA microarray of Yersinia pestis was used as a tool to perform genomic comparison among live plague vaccines prepared with 19 different strains. Results Dozens of deletions and/or increased copies of the genomic fragments were identified in the studied vaccines of different strains. Conclusion The revealed genomic differences among the vaccines from different origins account for the variability of the immunogenic and protective potency of live plague vaccines. The whole-genome DNA microarray was also provesd to be an ideal tool for the pre-evaluation of a vaccine strain.