Objective: To evaluate the noise characteristic of different reconstruction type CT image by using the noise power spectrum (NPS), and analyze the correlation between this results and subjective vision noise evaluation. Methods: QA water phantom of CT equipment was scanned and 8 common reconstruction algorithms were applied to dispose image. NPS peak value, the peak frequency and standard deviation (SD) were compared with the subjective evaluation measurements, such as granularity, contrast, sharpness and optical noise level, by using the correlation analysis. Results: Each reconstruction algorithm owned different peak value and peak frequency. Granularity negatively correlated with the peak frequency. A positive correlation was found between contrast and peak value. Sharpness was positively correlated with both peak value and peak frequency. All of the subjective evaluation measurements were correlated with SD. Conclusion: Comparing with SD, NPS can reflect both intensity and morphological feature of the noise and possess applicative potential as a more comprehensive evaluation index.

AIM: To explore the effects of sitai tablet on blood pressure, blood lipid,blood sugar and insulin of rats with insulin resistance syndrome(IRS).METHODS: A total of 40 male clean SD rats were divided into normal control group, model group, sitai group and captopril group by randomized block design. The rats were fed with high-fat high sugar diet for 8 weeks to induce IRS. Then, sitai tablets were given to rats with IRS to observe the influence of sitai tablet on blood pressure, blood sugar, blood lipid, insulin levels and insulin sensitivity index(ISI) of them.RESULTS: The systolic pressure reduced from ( 158 ± 8), ( 157 ± 7) mm Hg before treatment to (128 ± 4), (110 ± 2)mm Hg after treatment in sitai group and captoril group respectively. There was also significant difference on systolic pressure of between sitai group, captopril group and model group [ ( 165 ± 6) mm Hg] ( t = 11.86 - 28.99, P < 0.01 ). In sitai group, the total cholesterol (TC), triacylycerol (TG)decreased clearly than those in model group( t = 44.27, 9. 14, P < 0.01 ), but high density lipoprotein cholesterol (HDL-C) increased( t = 703, P < 0. 01 ) . There were increased fasting insulin(FINS) and decreased ISI in model group. There was significant difference on these indes between model group and normal control group( t= 7.14, 9.35, P < 0.01 ) . The insulin resistance was evident so that the model was successfully established. The fasting blood sugar and FINS all reduced when comparing it between sitai group, captopsil group with model group, there was significant difference( t = 11.95, 6.71, P < 0.01). When comparing the fasting blood sugar in captoril group and sitai group, there was also difference( t = 8.33, P < 0.01 ) . The ISI all increased in sitai group and captoril group, there was difference between them and model group (t=9.82,9.35, P <0.01).CONCLUSION: Sitai tablet can remarkably reduce the blood pressure,blood sugar and blood fat, and increase the sensibility of insulin so that it can improve the general effects of IRS.

Objective To evaluate the subject's absorbed dose,equivalent dose and effective dose.Methods The CBCT unit was Implagraphy and three scan projections were selected such as mandible,maxilla and temporamandibular joint (TMJ).Thermoluminescent dosimeter tubes were used to record the absorbed dose at special positions in the head and neck region of an adult skull and tissue-equivalent phantom.16 interested organs included pituitary,lens,parotid glands,submandibular glands,sublingual glands、diploe,spongy bone of the chin and cervical vertebra,skins of cheeks and nuchal region,thyroid and esophagus.The absorbed dose was measured in these organs,and then the effective dose(E1990,E2007)were calculated according to different ICRP tissue weighting factors.Results The absorbed dose of mandible,maxilla and TMJ scan varied from (0.99 ±0.09) to (12.85 ±0.09)mGy,(0.93 ±0.01) to (13.07 ±0.02) mGy and (0.68 ±0.01) to (10.18 ± 0.04)mGy.There was significant difference among the three scan projections (F =19.61-30992.27,p ＜ 0.05).The equivalent doses of lens and skin were (1.11± 0.07)-(5.76 ± 0.06) mSv and (6.96 ± 0.06)-(10.64 ± 0.07) mSv.There was significant difference among the three scan projections(F =4473.02,9385.50,P ＜0.05).The effective dose(E1990,E2oo7) was [(191.35±1.53),(325.17 ±2.58) μSv] for mandible scan,[(106.62 ±2.17),(226.28 ±2.81)μSv] for maxilla scan,[(104.21 ± 1.02),(142.36 ± 1.90) μSv]for TMJ scan,respectively.Conclusions The valid measurement should be taken to reduce the subject' s dose such as a careful history and clinical examination before the performance of CBCT,the latest risk/benefit assessment,precise scan position,the shielding of thyroid as well as brain and the smaller volume size as well.

Objective To investigate the clinical value of computer-assisted navigation system (CANS) in pedicle screw placement. Methods From August 2002 to June 2006, we carried out 66 cases of pedicle screw placement under the guidance of CANS; 66 cases of traditional pedicle screw placement were set as a control. Results The mean operation time of CANS group was significantly shorter [(142.3?5.3) min vs (173.4?7.1) min; t=-28.301, P=0.000] and the blood loss was significantly fewer [(798.3?10.9)ml vs (912.2?14.3) ml; t=-51.463, P=0.000] than those in the control. X-ray and CT scan respectively showed that the placement of pedicle screw in CANS group was significantly more accurate than that in the control. (?2=29.424, P=0.000; ?2=36.829, P=0.000). Conclusion Pedicle screw placement under the guidance of CANS is more accurate, safe, and micro-invasive than traditional operation.

Objective To observe the effect of Yinchenwulin(YCWL) powder on the c-myc rnRNA expression in vascular smooth muscle cell (VSMC) of atherosclerosis(AS) rats. Methods 70 Wistar rats were randomly divided into four groups: normal, model, Gypenosides treatment and YCWL powder treatment groups. AS models of rats were set up by feeding with high fat diet for one month. One month after treatment the level of serum lipid and blood rheology were measured, the phathological change of aorta was observed by HE staining, the ultrastructure change of aorta was observed by transmission electronic microscope, and c-myc mRNA expression in VSMC was detected by RT-PCR. Results Compared with Gypenosides treatment, YCWL powder could obviously decreased the levels of serum TC,TG and LDL, increase serum HDL level, reduce plasma viscosity,whole blood mid-cut,hematocrit and platelet adhesion rate, maintain aorta tissue structure, and down-regulate c-myc mRNA expression. Conclusion YCWL powder has therapeutic effect for atherosclerosis by regulating c-myc mRNA expression.

Objective To observe the clinical effect and safety of umbilical cord blood mesenchymal stem cell(UCB-MSC) transplantation in the treatment of diabetic foot. Methods UCB-MSC suspension (cell concentration (3 -7) × 107/L,0.3 -0.5 ml per point) was injected into multiple spots on affected lower limb with a 3 cm × 3 cm istance among each point. Demixing injection could be performed in regions with multilayer muscles. Clinical symptoms and related index were routinely observed from the first day to three months after operation. Results After three months of UCB-MSC transplantation, pain of patients was relieved, skin temperature increased, intermittent claudication ameliorated, ulcer healed, ankle-brachial index and transcutaneous partial pressure of oxygen increased. The lower extremity lesions showed an abundant collateral vessel formation after the treatment in 2 patients by angiography. Both patients had no severe complications and adverse reactions, none underwent amputation. Conclusions Umbilical cord blood mesenchymal stem cell transplantation is a safe and effective treatment of diabetic lower limb ischemic disease, which can exempt the patient from amputation and improve their quality of life.

Objective To establish a military disease prevention and control website to achieve dissemination of disease control information , resource sharing , business communication in the whole army , and popularize relevant knowledge of disease prevention and control promotion among the troops .Methods Based on the content management system and meta-data repository platform,B/S system architecture, JSP,MySQL and other programming techniques and tools were used to complete the system development .Results The military disease prevention and control website we designed has been for-mally launched ,which was able to implement a set of functions such as content distribution , full-text retrieval ,classification and aggregation of knowledge , online consultation .Conclusion This website plays a positive role in enhancing military prevention and control ,popularizing relevant laws and regulations ,meeting the health needs of the army soldiers and impro-ving the ability of disease prevention in grass-roots units.

Objective To investigate the effects of allogenic intra-bone marrow bone marrow transplantation (IBM-BMT) on re-establishing hematopoiesis in mice. Methods Bone marrow mononuclear cells (BMNCs) from BALB/ c mice were transplanted into the C57BL/6 mice treated with a lethal dose of ~(60)Coγ-ray radiation through intra-bone marrow injection or intravenous injection. Sixty of the C57BL/6 mice were randomly divided into three groups as higher dose intra-bone marrow injection group (IBM1 group), lower dose intra-bone marrow injection group (IBM2 group) and intravenous injection group (IV group). The nucleated cell numbers of whole bone marrow from the tibia of each recipient mouse were counted respectively at the day 1, day 3, day 6 and day 9 after the transplantation. The donor-derived total nucleated cells and myeloid cells were quantified by flow cytometry. Results At 6th day after transplantation, more total bone marrow nucleated cells, total donor-derived nucleated cells and donor-derived myeloid cells in the tibia of injected side in both IBM1 group and IBM2 group were found than that in IV group (P<0.05 or P<0.01). Conclusion Compared with traditional bone marrow transplantation (IV-BMT),IBM-BMT improves the bone marrow hematopoiesis in the early hematopoietic re-establishing stage in allogenic bone marrow transplantation.

OBJECTIVE: To analyze the clinical significance of ocular vestibular evoked myogenic potentials (oVEMP) and cervical vestibular evoked myogenic potentials (cVEMP) in primary unilateral benign paroxysmal positional vertigo (BPPV). METHOD: Fifty-two patients with unilateral primary BPPV (BPPV group) and 38 normal subjects (control group) received ocular vestibular evoked myogenic potential (oVEMP) and cervical vestibular evoked myogenic potential (cVEMP) test using tone burst stimuli. The response rate, latency and amplitude were analyzed. RESULT: In BPPV group, the response rate of oVEMP was 46.15% in lesioned side and 48.08% in healthy side, respectively. The response rate of cVEMP was 67.31% in lesioned side and 65.38% in healthy side, respectively. In control group, the response rate on the left ear was 84.21% for oVEMP and 92.11% for cVEMP. On the right ear, was 81.58% for oVEMP and 94.74% for cVEMP in control group, there was no significant difference in cVEMP and oVEMP P1, N1 N1-P1 latency and amplitude between left and right ear. The interaural amplitude ratio and asymmetry of cVEMP and oVEMP was significantly different between BPPV group and control group (P<0.05). CONCLUSION Unilateral primary BPPV with bilateral impaired vestibular otolith pathways function can be objectively evaluated by oVEMP and cVEMP detection. Abnormal oVEMP responses were more frequently detected than cVEMP.
Adult ; Benign Paroxysmal Positional Vertigo ; physiopathology ; Case-Control Studies ; Female ; Humans ; Male ; Middle Aged ; Prospective Studies ; Vestibular Evoked Myogenic Potentials ; physiology ; Young Adult

Background and purpose:Breast cancer is one of the most common carcinoma among female patients with high mortalities. Drug-resistance is the major reason that leads to chemotherapy failure in clinical practice. MCF-7/ADR is a multi-drug resistant cell line that was established on the basis of breast cancer cell line MCF-7. This research aimed to investigate the anti-apoptotic effect of c-fos in resistant breast cancer cell MCF-7/ADR, and to compare with its sensitive counterpart MCF-7.Methods:Doxorubicin with various concentrations was used to treat MCF-7 as well as its MDR- counterpart MCF-7/ADR. The growth inhibitory rate of MCF-7 and MCF-7/ADR wasdetermined by MTT assay. Additionally, RT-PCR was used to test the expression of P-gp and c-fos mRNA in MCF-7 and MCF-7/ADR; The expression of c-fos mRNA was detected by RT-PCR after 3 μmol doxorubicin treatment;We further established cell lines that stably interfered with c-fos, named MCF-7/ADR/si-fos-8B, MCF-7/ADR/si-fos-3D. Flow cytometry and MTT assay were used to investigate the apoptosis rate and inhibitory rate in these above cells under the treatment of 5-FU, CDDP or γ-radiation. At last, RT-PCR and Western blot analysis were used to detect the expression of bax, bcl-2, puma, p53.Results:The expression of c-fos and P-gp (MDR-1) was up-regulated in MCF-7/ADR, compared with its sensitive counterpart MCF-7. Additionally, the resistant fold of MCF-7/ADR to doxorubicin was nearly 40; The expression of c-fos was gradually up-regulated after 3 μmol doxorubicin treatment; The sensitivity to drugs (5-FU and CDDP) was increased after c-fos interference while the apoptosis rate was also increased after 5-FU, CDDP and γ-radiation treatment. RT-PCR and Western blot analysis indicated that up-regulation ofbax,puma,p53 after c-fos interference while the expression of bcl-2 was down-regulated.Conclusion:c-fos may act as an anti-apoptotic protein in resistant breast cancer cell line MCF-7/ADR by regulating the expression of apoptosis related proteins, and may play a vital role in the formation of multi-drug resistance phenotype.