1.Comparison of Two Commercial Enzyme-linked Immunosorbent Assays for Detection of IgG Antibody to Diphtheria
Xin ZHAO ; Dongsheng REN ; Li XU
Chinese Journal of Vaccines and Immunization 2008;0(01):-
Objective To compare the performance of two commercially available ELISA kits for detection of diphtheria IgG antibody to provide data for the proper kit choice. Methods Thirty-three serum samples from staff of the capital airdrome and two standard sera were tested for the evaluation of the kits.Commercial ELISAs were compared with respect to their reproducibility(intra-assay variation and inter-assay variation)and accuracy.Results For the detection of standard serum,intra-assay variation(Coefficient of Variation,CV)was 13.5507% for IBL kit and 5.9541% for Virion kit,the inter-assay variation(CV)was 9.9890% for IBL and 2.4728% for Virion kit.For the serum sample,intra-assay variation(CV)was 14.5270% for IBL and 11.0612% for Virion,the inter-assay variation(CV)11.0039% for IBL and 8.1506% for Virion kit.For the detection of standard serum,inter-assay variation(CV)was 9.9890% for IBL and 2.4728% for Virion.The accuracy was analyzed by variation between the actual concentration and measured concentration of standard serum,the CV was 17.6889% for IBL and 1.3966% for Virion.Conclusion The intra-assay and inter-assay variation of the two kits are generally acceptable(CV
2.Evaluation and Countermeasures on OTC Cough and Cold Medicines for Children in New Zealand
Dongsheng XIA ; Gang CHENG ; Ren TANG
China Pharmacy 2005;0(22):-
OBJECTIVE:To introduce positive response to the security and effectiveness issues on OTC cough and cold medicines for children in New Zealand in order to provide reference for drug risk-benefit evaluation and putting forward reasonable management measures in China. METHODS:The concept of OTC cough and cold medicines for children were introduced firstly. Then the evaluation and countermeasures of OTC cough and cold medicines for children in New Zealand were also introduced. RESULTS & CONCLUSIONS:It has significance for the evaluation of drugs for sale that are review and evaluation,study and countermea-sures and dynamic tracking for OTC cough and cold medicines for children in New Zealand and the realistic attitude of New Zealand Drug and Medical Instruments Security Bureau.
3.Effects of low-frequency ultrasound combined with Tongluo Zhitong herbs on urokinase-type plasminogen activator system and inflammatory cytokines in knee osteoarthritis
Hong ZHANG ; Dongsheng REN ; Yafei TAO
Chinese Journal of Tissue Engineering Research 2017;21(8):1149-1154
BACKGROUND: Low-frequency ultrasound combined with Tongluo Zhitong herbs can effectively relieve pain and improve joint function of patients with osteoarthritis, but the underlying mechanisms are still unclear.OBJECTIVE: To investigate the effects of low-frequency ultrasound combined with Tongluo Zhitong herbs on urokinase-type plasminogen activator (uPA) and inflammatory cytokines of osteoarthritis rabbits and the related mechanisms.METHODS: Forty rabbits were randomly divided into four groups: normal, model, control, and experimental groups. The normal group was treated with normal feeding, not for any processing. For the other three groups, papain was injected into the rabbit knee articular cavity to establish osteoarthritis models. One week later, the experimental group was treated with low frequency ultrasound combined with Tongluo Zhitong herbs for 3 weeks, the control group treated with the same volume of normal saline, and the model group received no intervention. The levels of inflammatory cytokines in the serum and synovial fluid were measured, the histological changes of synovial tissues and articular cartilage were evaluated using Mankin's score, and the expression levels of uPA and its receptor were determined by western blot assay.RESULTS AND CONCLUSION: The levels of inflammatory cytokines in the model group were significantly higher than those in the normal and experimental groups (P < 0.05). The Mankin's scores of synovial tissue in the experimental group were significantly lower than those in the model and control groups (P < 0.05), while Mankin's scores of the cartilage tissue had no significant difference between the experimental and model groups (P > 0.05). Compared with the model group, in the experimental group, the expression levels of uPA and its receptor were significantly downregulated,and the expression of uPA inhibitor was notably upregulated. These data show that low-frequency ultrasound combined with Tongluo Zhitong herbs attenuates inflammatory reaction in the synovial tissues, by inhibiting the formation of joint fluid and production of inflammatory cytokines, which is an effective treatment for knee osteoarthritis.
4.The clinical value of IgG antibodies avidity index detection in pregnant women with TORCH infection
Dongsheng WU ; Yuanjian WU ; Yalu REN ; Liuhong WANG
International Journal of Laboratory Medicine 2014;(5):538-539
Objective To explore the relationship between the type of TORCH infection and pregnancy outcome through the de-tection of TORCH infection in pregnant women with IgG avidity index (AI) .Methods Collected each type of TORCH pathogens IgM positive maternal serum ,and detect the IgG avidity index and track the outcome of pregnancy .Results The rate of TORCH pathogens recent infection (AI<30% )in pregnant women ,HSV ,RUB ,CMV ,TOXO respectively were 0 ,3 .8% ,9 .3% ,7 .9% ;In the group of pregnant women with TORCH-IgG AI<30% ,there were 2 cases of pregnancy outcome of abnormal (missed abortion) and one case of fetal malformations (hydrocephalus) .Conclusion In the recent infection of TORCH pathogens in pregnant women , mainly are non-primary infection ;The damage of cytomegalovirus and Toxoplasma gondii primary infection to the fetus is much larger than the non-primary infection .
5.Research progress of animal models of multiple sclerosis
Yingxia LI ; Dongsheng GUAN ; Deqi REN ; Jian GUO ; Chuanjie WU
Acta Laboratorium Animalis Scientia Sinica 2015;23(5):535-538
Experimental autoimmune encephalomyelitis ( EAE ) is a classic animal model for multiple sclerosis . After more than 2 centuries of development , EAE can be established in mice , monkeys and rats ,etc.Some animals can be immunized by different immune antigens which have different features in sensibility and clinical characteristics .In this pa-per, we will review the development of EAE , discuss the characteristics of EAE models produced by immunization of differ-ent animals with different antigens .In summary, we explore the development of animal models of EAE .
6.Construction of a three-dimension model on evaluation of human resources for military medical sciences
Pan LI ; Dongsheng ZHAO ; Jun REN ; Songjun WANG
Military Medical Sciences 2014;(9):690-693
Based on a correct understanding of the related concepts and system of the evaluation of human resources for military medical sciences ( EHRMMS), this thesis established a three-dimension model of EHRMMS that is composed of the evaluation goal, evaluation subject and evaluation object, and analyzed the factors that affect the crucial parameters in the model.We also analyzed the features and regularity of the evaluation model, and tentatively explored the application of the model.
7.Role of PKCα-Nrf2-HO-1 signaling pathway in endotoxic shock-induced acute lung injury in rabbits
Guoyan LIU ; Xiaodong LIU ; Jianbo YU ; Lirong GONG ; Dongsheng REN
Chinese Journal of Anesthesiology 2015;(5):620-623
Objective To evaluate the role of protein kinase Cα( PKCα)?nuclear factor E2?related factor 2 ( Nrf2)?heme oxygenase?1 ( HO?1) signaling pathway on endotoxic shock?induced acute lung injury ( ALI) in rabbits. Methods Thirty healthy male New Zealand white rabbits, aged 2 months, weighing 2?0-2?5 kg, were randomly divided into 3 groups ( n=10 each) using a random number table: normal control group ( group C);ALI group ( group ALI);PKCα inhibitor chelerythrine group ( group CHE) . In group CHE, chelerythrine 8 mg∕kg ( in 0?5 ml of DMSO) was injected intraperitoneally, and 30 min later, LPS 5 mg∕kg ( in 2 ml of normal saline) was injected via the auricular vein to induce ALI in ALI and CHE groups. The rabbits were then sacrificed at 6 h after injection of LPS or normal saline, and the lungs were removed for examination of the pathological changes which were scored and for determination of wet∕dry lung weight ratio ( W∕D ratio) , and the expression of Nrf2 and HO?1 protein and mRNA. Results Compared with group C, the pathological score and W∕D ratio were significantly increased, and the expression of Nrf2 and HO?1 protein and mRNA was up?regulated in ALI and CHE groups. The pathological score and W∕D ratio were significantly higher, and the expression of Nrf2 and HO?1 protein and mRNA was lower in group CHE than in group ALI. Conclusion The PKCα?Nrf2?HO?1 signaling pathway is one of the endogenous protective mechanisms underlying endotoxic shock?induced ALI in rabbits.
8.Relationship between serum PAPP-A expression and gene polymorphism with severity of coronary lesions in patients with coronary heart disease
Hui LIU ; Haijian LI ; Chuanyu GAO ; Shaofen MAO ; Yafei TAO ; Dongsheng REN
Chongqing Medicine 2016;45(18):2488-2490,2493
Objective To investigate the relationship between serum pregnancy associated plasma protein A (PAAA‐A) ex‐pression and gene polymorphism with the severity of coronary lesions in the patients with coronary heart disease (CHD) .Methods Ninety‐eight patients with CHD in the Nanyang Municipal Central Hospital were selected as the observation group and divided into single vessel lesion group and multiple vessel lesion group according to coronary angiographic results .Ninety‐eight individuals un‐dergoing healthy physical examination were selected as the control group .The venous blood was collected at the visiting hospital in the observation group and at the physical examination in the control group for detecting the serum PAPP‐A protein level by ELISA .PAPP‐A gene and IVS6+ 95 polymorphism were analyzed by adopting polymerase chain reaction restriction fragment length polymorphism(PCR‐RELP) .Results Compared with the control group ,peripheral blood PAPP‐A protein level in the obser‐vation group was significantly increased(P<0 .05) ,moreover the PAPP‐A protein level in the multiple vessel lesion group was sig‐nificantly higher than that of the single vessel lesion group (P<0 .05) .The peripheral blood PAPP‐A level was positively correlated with the severity of CHD .Three genotypes existed in PAPP‐A gene IVS6+95 locus ,including CG heterozygous ,homozygous CC and GG homozygous type .The CC homozygous allele frequency in the patients with multiple vessel lesion was higher than that in the patients with single vessel lesion (P<0 .05) .Conclusion The PAPP‐A protein level and IVS6+95 polymorphism have a close relation with the severity of coronary lesions in patients with CHD .CC genotype may be one of genetic susceptibility gene markers in the patients with CHD .
9.Rociverine combined with tamsulosin in treatment of overactive bladder after transurethral resection of prostate
Cheng LIU ; Jiagui MU ; Duo LIU ; Xuejun LIU ; Kai LU ; Yansheng REN ; Dongsheng ZHU
Journal of Endocrine Surgery 2013;7(4):319-321
Objective To study the efficacy and safety of rociverine combined with tamsulosin in treatment of overactive bladder after transurethral resection of prostate (TURP).Methods 93 cases of overactive bladder patients who received TURP were collected.They were given rociverine tablets 10 mg 3 times a day and tamsulosin 0.2 mg once a day for 7 to 10 days.The efficacy and safety of treatment were assessed by changes before and after treatment in subjective indicators of overactive bladder syndrome score(OABSS),the international prostate symptom score(IPSS) and quality of life score(QOL) and objective indicators including daily frequency of micturition,urgency,urgency incontinence,nocturia,voiding volumes,combining with the adverse drug reaction incidence.Results The average treating time for the 93 cases was (5 ± 3) days.The average score of OABSS reduced 8.12(P <0.01,and each score≤ 4).The average score of IPSS reduced 16.69(P <0.01).The average score of QOL reduced 2.18 (P < 0.01).There was statistical difference in objective indicators of patients between before and after treatment(P < O.05).The adverse drug reaction incidence was 2.1% (2 cases with thirsty symptom).Conclusion Rociverine tablets combined with tamsulosin in treatment of patients with overactive bladder after TURP are effective and safe.
10.Effect of the ongoing time of applying Musk Xiaochuan paste in stable asthma
Dongsheng GAO ; Yajun ZHANG ; Youjun REN ; Guangyuan YANG ; Yan HUANG ; Liping ZHAO ; Zhonghao NAN
Chinese Journal of Biochemical Pharmaceutics 2016;36(4):108-110
Objective To explore the effect of the ongoing time of applying Musk Xiaochuan in the treatment of stable asthma and obtain the optimal therapy.Methods 129 cases of patients with stable asthma were randomly divided into 3h group (29 cases), 4h group (34 cases), 5h group (31 cases), 6h group (35 cases) according to the different applying time.The selection of points, applying frequency, treatment course remained the same, and then “dog day” was selected to apply Musk Xiaochuan paste for treatment.The asthma quality of life and asthma control pre-and post-treatment were observed.Results After applying, the asthma control questionnaire (ACQ) score difference value in 3h group was not preferable than the other groups, with no significant difference.The asthma quality life questionnaire (AQLQ) total score difference value in 4h group was significantly higher than the other 3 groups, with significant difference(P<0.05).The activity limitation score difference value and mental health difference value of AQLQ in 4h group were significantly higher than the other 3 groups, with significant difference(P <0.05), the asthma symptom score and stimulus response score difference values, health care for their own score difference value also showed significant effect (P<0.05).Conclusion The difference of the ongoing time of applying Musk Xiaochuan has different efficacy in the treatment of stable asthma, the efficacy of applying for 4h is better than 3, 5,6 h, while it could not determine the best time in asthma control.