Objective To evaluate the diagnostic value of myocardial enzymes on the cardiac muscle injury for the children with acute mycoplasma pneumonia(MP).Method The levels of phosphocreatine kinase-MB(CK-MB),lactate dehydrogenase(LDH),aspartate aminotransferase(AST)in 48children with MP(MP group)were measured,48 normal children served as control group.Results The levels of AST,LDH,CK-MB were(37.18±6.15),(294.10±46.20),(84.50±13.70)U/L in acute stage in MP group,they were higher than those in control group[(25.14±10.50),(140.50±76.21),(19.50±4.10)U/L](P＜0.01).All the values above was descended in recovery phase in MP group,but the values of CK-MB[(27.80±8.40)U/L]and LDH[(189.50±69.73)U/L]were still higher than those in control group(P＜0.01 or＜0.05).Conclusions Children with MP may combine myocardial injuries and CK-MB isa parameter to diagnose the damage.Protection therapy for cardiac muscle should be performed on the children with MP.

ADAM9, a member of ADAM family, plays an important role in development of many human diseases especially neoplasms as its metaUoproteinases activity and the ability of combining with integrins. This might help us find new therapeutic targetpoints and therefore offer new therapeutic approaches.

Objectives To evaluate the application of systemic octreotide radioactive imaging in diagnosis and follow-up for children with neruoblastoma (NB). Methods Clinical data of 14 children with NB applied systemic octreotide radioac-tive imaging from April 2006 to March 2011 were collected and analyzed. For the ifrst-visit patients, they were applied with systemic octreotide radioactive imaging at the ifrst follow-up appointment after routine treatment. And for recurrent or sus-pected recurrent cases, systemic octreotide radioactive imaging was applied before the treatment and after 2-3 weeks chemo-therapy. Results Nine of 14 patients were male and 5 were female with a median age of 4.8 years (1-10 years). According to In-ternational Neuroblastoma Staging System (INSS), 4 cases were categorized as stage 3 and 10 cases were categorized as stage 4. The uptake rate of systemic octreotide radioactive imaging in primary site or metastasis sites of 8 cases were higher than nor-mal site with positive rate of 57.14%(8/14). One case was conifrmed as false positive by treatment and imaging examination. Six cases were negative in primary site or metastasis sites with negative rate of 42.86%(6/14). One case was conifrmed as false negative via treatment and imaging examination. Therefore, the sensitivity of systemic octreotide radioactive imaging reached 85.71%(12/14). Conclusion Systemic octreotide radioactive imaging could be one of the effective methods for NB in diagno-sis, follow-up and evaluation of prognosis.

B7H1 is a recently described B7 family member.Ligation of PD-1 receptors with B7hl on the surface of activated T cell inhibits T cell proliferation and cytokine production. B7H1 plays a negative regulatory role in peripheral tolerance, autoimmune diseases and chronic infections.B7H1 also has been found to be largely expressed on a broad range of cancers and is thought to contribute to immune evasion by cancers. Blockade of B7H1/PD-1 pathway may contribute to the treatment of autoimmune diseases and maligant tumors.

AIM To investigate the mechanism of anti-tumor effects of curcumin on human SPC-A1 cells. METHODS Cell culture、fluorescence microscope、TUNEL method and irradiation immunity techniques were used. RESULTS ①By the action of curcumin, SPC-A1 cells grew against the wall and then peeled off gradually;fluorescence microscope showed the nucleus broke into pieces, and cell split into apoptosis bodies in different sizes; The TUNEL method further certified that apoptotic cells reached 42 67% at the concentration of 20 ?mol?L -1 curcumin at 24 h. ②The level of intracellular cAMP concentration in the SPC-A1 cells,treated with curcumin, was significantly higher than that of the control group. ③Treated with 20 ?mol?L -1 curcumin for 24 h, the expression of Caspase-8 mRNA of human SPC-A1 cells was up-regulated. CONCLUSION Curcumin can induce the apoptosis of SPC-A1 cells, and one of its molecular mechanism may be associated with the rising of the level of intracellular cAMP concentration, and up-regulating expression of Caspase-8.

0.05). However,the accuracy in placement pedicle screws and derotation rate of apical vertebrae were much higher in the individually treated group than those in the standard group (P=0.001,0.02).[Conclusion]The accuracy in placement pedicle screws and correction effects on transverse plane of patients with Lenke Ⅰ AIS can be improved significantly by individual selection of entrance point.

Objective To find out the differences of sleep posture between normal children and those with obstructive sleep apnea hypopnea syndrome(OSAHS),and assess the effects of sleep posture on the children with OSAHS.Methods The results of polysomnography(PSG) of 385 children diagnosed as OSAHS by polysomnography were compared with that of 161 children who matched the former in age and gender(Controls).The effects of different sleep posture on obstructive apnea,hypopnea index(AHI) and apnea index(AI) were analyzed.Results For the children with OSAHS when sleeping,the percentages of sleep time in different posture were 45.4%+36.3% in supine posture,39.5%+34.5% in side posture and 15.1%+32.6% in prone posture,respectively;while in control group,the correspond percentages were 32.8%+24.5%,54.7%+23.9% and 12.5%+17.2%,respectively.Comparing the two groups,significant differences existed on the sleep time in both supine and side posture(P0.05).Children with OSAHS spent more time in supine posture and less time in side posture than those children in control group(P0.05).For the children with OSAHS,the average AHI and obstructive AI in three sleep-related postures were 14.5 and 3.80,respectively.The values of both AHI and AI in supine posture were significantly greater than that in side or prone posture(P

This paper studies the application of hyaluronic acid to artificial bone,manmade skin and restored nerve,and points out that the self-regulated releasing hyaluronic acid biomaterial will become a hot issue.

Objective To analyze the clinicopathological features and enhance the knowledge of Askin tumor in children. Methods The clinicopathological features of 4 cases of children with Askin tumor were described. Results Clinically, the tumor was often seen in elder child patients with painful thoracopulmonary masses.Histopathologically, the nest-like or lobular structures composed of small round-to-oval cells were surrounded by sparse fibrovascular stroma. Among tumor cells there were no or few resticular fibers,however in the surrounding of tumor masses the fibers were found. PAS staining in the tumor in a part of cases was positive. The positive rates of CD99 and Syn detected by immunohistochemistry were high. Neurosecretory granules can be observed by electron microscopy. Two patients received both chemotherapy and radiotherapy three patients died of metastatic tumor of lung and brain and chemotherapy complication in 24,25 and 5 months, respectively. Conclusions Askin tumor is a highly malignant neurosecretory tumor in thoracopulmonary region, which often be misdiagnosed as neuroblastoma, lymphoma, embryonal rhabdomyo sarcoma, tuberculosis and lung abscess, and requires a synthetical treatment plan consisting of pre-and postoperative chemotherapy, radiotherapy and surgical resection. The treatment methods of Askin tumor needs to be further studied.

20?10 9/L) were 12 (8-20), 17(10-28) and 21 days (7-78), respectively. The average follow-up period was 7 3 years(ranged from 1 month to 14 years). The overall survival (OS) and death rates after transplantation were 70%(18/26) and 30%(8/26) respectively. Transplant-related mortality was 7 7%(2/26). Conclusion The combination of chemotherapy and G-CSF is an effective and low toxicity protocol for the mobilization of peripheral blood stem cells. APBSCT can be safely performed in children with malignant tumors.