Objective:To study the protective effect of Ginkgo biloba L.extract(EGB) on PC12 cell damage induced by rotenone and 1-methyl-4-phenylpyridium(MPP +). Methods: PC12 cells damage model was induced with different concentrations of rotenone and MPP + and were treated with EGB . Then cell morphology was observed; survival rate and ultracellular dopamine (DA) level were determined by MTT assay and ELISA assay, respectively. Results: MPP + -induced damage was attenuated by pre- or co-treatment with EGB at 0.35,0.70 and 1.40 mg/ml, while rotenone-induced damage was attenuated with EGB only at 1.40 mg/ml.The DA concentrations of extracellular at 2 mmol/L MPP + (6.875?0.201) ng/ml and 10 ?mol/L rotenone (5.321?0.167) ng/ml increased to ( 7.595?0.139 ) ng/ml (P