In majority of malignant lymphoma patients the overexpression of mutant p53 gene can be found,and experiments show that p53 gene and the genesis currence and development of malignant lymphoma are closely related.Thus,normal p53 gene carried by recombinant adenovirus vector into tumor cells and its stable expression will play a theraputic role in the treatment of malignant lymphoma.This has been confirmed by the basic research.

Objective:To investigate the influence of curcumin on the level of nitric oxide in kidney and the renal protective effect in diabetic rats.Methods:Diabetic models were induced by a single intraperitoneal injection of streptozoticin(STZ)into male Wistar rats at a dose of 60mg/kg body weight and was randomly divided into two groups:untreated diabetes group(group DM,n=10) and diabetes group treated with curcumin 200mg?kg -1 ?d -1 (group Cur,n=10).A normal contral group(group N,n=10)was also designed,After treated for eight weeks,the level of NO in kidney,renal structure and function were evaluated.Results:The levels of urinary albumin excretion rate (UAER),albumin-creatinine ratio(Alb/Cr),kidney weight(KW),profile of kidney hypertrophy(KW/BW),mean glomerular area (MGA) and mean glomerular volume(MGV)were all significantly increased in dialetic rats as compared with normal rats (all P

Objective:To explore a new method and it's clinical efficiency of porcelain technique on severe tooth wear and dentition defect.Methods:The transitional occlusal pad restored the vertical dimension and centric relation.decreased TMD symptoms.The porcelain technique was used to reconstruct the occlusal relationship,their prognoses and patients' satisfactory were recorded.Results:45 patients suffered from serious dental abrasion,whose clinical symptoms got much better and masticatory efficiency improved significantly.Conclusion:Porcelain is an aesthetic and comfortable technique to improve patients' masticatory efficiency significantly which is similar to real tooth.

Objective:According to observe the 5-year clinical effect in the treatment of restoration of teeth with extensive de- fection through pulp chamber retainer,to discuss the usage and the defect of pulp chamber retainer.Methods:108 extensively damaged molars which had been finished pulp treatment were choosed.Retainers were fabricated correspond to the pulp chamber then full crow were factured.Results:108 molars were restored by full crown restoration with pulp chamber retainer,in which only 7 cases fell off.Conclusion:The retainer in pulp chamber can be used in the treatment of teeth with extensice defection and makes it easier in clinical operation.

Objective; To explore a new method and its clinical significance of restorative ceramo - metal by using cast metal post -core to reutilize the broken crown. Methods; 30 teeth were restored with ceramo - metal tooth by using cast metal post - core in order to preserve the old broken base tooth, and their prognoses were observed. Results: 30 teeth post - core were inserted smoothly, and PFM crowns border sealed. During 5 years follow up ,93% (28 teeth) cases were successful, although unfortunately there were 2 cases of failure with split of tooth root. Conclusion; Restoration of ceramo - metal tooth by cast metal post - core in order to reutilize the broken crown of the base tooth is convenient ,time - saving and well casted ,therefone it could be used widely in clinic.

Contrasuppressor T cells (TCS) take part in immunoreglation by rendering T help cells re-sistant to the effects of suppressor cells.It was found that both human and mouse TCS expressreceptor for vicia villola letcin (VVR).Human effective TCS are mainly CD8VV~+ T subsetswhich are closely associated with abnormalities of immune activity of many diseases.By usingroutine and double immunofluorescent labelling techniques combined with flow cytometry,dif-ferentVV~+ subsets were analysed.A significant increase was observed for CD8VV~+ cells in ac-tive SLE (23.6%)as compared with the control group (8.5%) and the SLE patients in remis-sion (14.49%).The percentage of CD4VV~+ subset,however,was unchanged.Moreover,thetypical contrasuppressive activities exerted by VV~+ cells,which were also increased significantlyin active SLE patients,were detected in SLE in a dose—dependent manner.In an experimentwith PPD—induced lymphoproliferation as indicating system,for example,the relative respons-es were observed to be increased doses in from 100% to 485% and 625% after the VV~+ cellswere added back in increased doses,suggesting that the contrasuppressive VV~+ or CD8VV~+cells were added back in increased doses,suggesting that the contrasuppressive VV~+ or CD_8VV~+cells Were closely related to the immune disorders of SLE.

In the article of Great Medical Sincerity,Sun Simiao's discussion of the medical ethics and medical skill reflected the characteristics of his virtue ethics—the integration of internal training and external training.Internal training makes the request for the quality of doctor from the internal subjective mind.External training is specification for external behavior of internal culture.The effective integration of these two aspects reflects Sun Simiao's positive life value which unifies individual life and social life.

Objective To explore the role of medical insurance in the treatment of the coronary heart disease(CHD) out-patients with or without medicare. Methods 92 CHD out-patients from the medicare department and 92 CHD out-patients from general department of Shandong Taishan Chronic Disease Hospital were investigated and comparatively analyzed on their clinical data in the past 1 year,including the times of hospital visiting,compliance with doctor's instruction,blood pressure,serum lipids and blood glucose control and evaluation of treatment effect based on their clinical symptoms and ECG records.Results The compliance with doctor's instruction,blood lipids,blood pressure control of out-patients in medicare department were significantly better than those in general department(P

OBJECTIVE:To optimize the extraction conditions of total flavones from Pueraria Lobata by ultrasonic method.METHODS:The uniform design method was applied to investigate the effects of alcohol content,the quantity of solvent,extraction time,extraction temperature and the extraction times on the result of extraction.The content of total flavone of Pueraria Lobata was determined by ultraviolet-visible spectrophotometry.The extraction conditions were optimized with the extraction yield and the mass fraction of total flavone of Pueraria lobata in the extract as indexes.RESULTS:The optimal extraction conditions were as follows:extracting with 75% alcohol(20 mL?g-1) for 40 min(extraction time) at 68 ℃(extraction temperature) for a total of 5 times.CONCLUSION:The extraction process is reasonable and feasible and it provides theoretic basis for the industrialized production.

Objective To investigate the clinical values of loop electrosurgical excision procedure (LEEP) in diagnosis and treatment of cervical intraepithelial neoplasia(CIN). Methods Fifty-two patients with cervical intraepithelial neoplasia underwent LEEP following cytology, colposcopy and multiple biopsies, were treated by LEEP. The cure rate, the operative time, bleeding volume and patients reaction of LEEP to the cervical intraepithelial neoplasia were investigated during the follow-up. Results The effective rate was 98.07 % in the patients treated by LEEP. The effective rate was 100 % in the patients in grade CIN Ⅰ or CIN Ⅱ by LEEP. No patients in grade CIN Ⅰ and Ⅱ had recurrence during the follow-up 1 year. The mean operative time was 7.8 minutes. The mean bleeding volume was 10 ml. No secondary bleeding and post-operative infection occurred. All these surgical specimens were checked successfully for pathology diagnosis. Conclusion The advantages to use the LEEP to manage cervical intraepithelial neoplasia include its simpleness to handle, short operative time,less bleeding, less vaginal discharge, safety, and high cure rate. LEEP can offer intact sample for pathological diagnosis. LEEP electrotome is a very ideal therapy for the cervical intraepithelial neoplasia, and can block the development of precancerosis to infiltrating carcinoma effectively.