Objective A Halotherapy (HT) device for meeting the demands of human body,has been trial-produced to efficiently treat the respiratory diseases.Method By means of grains of salt having been processed in a series of ways,proper halospray has been made for the treatment of respiratory diseases.Result Y JM-1 desk-top Halotherapy device,meeting the demands of human body and featuring a good halospray size and space density,has been developed,which is highly efficient to the treatment of respiratory diseases.Conclusion Y JM-1 desk-top Halotherapy device is a novel and creative one for the treatment of respiratory diseases.

Objective To explore the application value of color Doppler ultrasound in screening of fetal con-genital heart disease and its significance.Methods 4 132 cases of the expectant mothers were selected,using color Doppler ultrasonography on the line screening of five section to detect fetal cardiac,to compare with the actual clinical situation and analyze the occurrence of fetal congenital heart disease.Results Among 50 cases(1.21%) of congeni-tal heart disease,28 cases were live births, accounted for 56.00%,13 cases of induced abortion, accounted for 26.00%,9 cases died,accounted for 18.00%.48 cases were detected by ultrasound,relevance ratio was 1.16%,the accuracy rate was 96.00%,the missed diagnosis rate was 4.00%.28 cases of live births undergoing surgical correc-tion,treatment effect was good,48 cases were detected,including 10 cases of ventricular septal defect,6 cases of tetral-ogy of Fallot,6 cases of atrial septal defect,5 cases of endocardial cushion defect,4 cases of single chamber,4 cases of persistent truncus arteriosus,3 cases right ventricular dysplasia,3 cases of tricuspid valve shift malformation,2 cases of persistent left superior vena cava,2 cases left ventricular dysplasia,2 cases of aortic arch interruption,1 case of rhabdomyoma.Conclusion Using color Doppler ultrasound detection for pregnant women can effectively detect severe congenital heart disease in the fetus,stop gravid timely,which can effectively improve the level of prenatal and postnatal care that is worth clinical application.

Objective To analyze the clinical effect of brevisapine injection on high sensitivity C-reaction protein level in the treatment of acute myocardial infarction.Methods The patients were randomly divided into treatment group(n=80)and control group(n=80).Both groups were treated with routine midication,but treatment group were given intravenous drip of 100 mg brevisapine once a day.for 14 days.80 paients who came for diagnosis were selected for healthy control group.The selqlm high sensitivity C-reaction protein levels were measured at 2 d,7 d,and 14 d by ELLSA in treatment group,control group and healthy control group.Mortality,heart function status (Killip class),revaseularization and clinical adverse cardic events including postinfartion angina,new arrhythmia,reinfarction and bleeding complication at 4 weeks were observed.Results Treatment group had a significantly high level of hs-CRP compared with healthy subjects[(6.37±1.43)vs.(2.17±1.12)mmol/L,P＜0.01]at the second day.The serum hs-CRP content of treatment group was obviously lower than that of control group(3.21±1.31)and(2.25±0.34)mmol/L vs.(5.87±1.16)、(3.97±1.21)mmol/L,(P＜0.01)]on the 7th,14th days;The revascularization rate of treatment group was obviously higher than that of control group[62.50%(50/80)vs.51.25% (45/80),P ＜0.01];There was no difference between the two groups in mortality(3.75% vs.4.10%,P ＞0.05).No difference was found in the rate of bleeding between the two groups(6.25% vs.7.50%,P ＞0.05);Patients with Killip class＜Ⅱ of treatment group was obviously higher than that of control group (83.75% vs 61.25%,P ＜ 0.05 );Clinical adverse cardic events rates at 4 weeks of treatment group were obviously lower than those of control group (P ＜ 0.01 ).No obvious adverse reactions related to the treatment group were observed.Condusion The serum hs-CRP level is in close relation with acute myocardial infarction,which is the risk factor.It is effective and safe of brevisapine injection in the treatment of acute myocardial infarction.The effects of brevisapine on acute myocardial infarction might be involved in decreasing the level of hs-CRP and inhibiting the vascular inflammatory reaction.

Objective The diolistic assay has been modified to make it simpler and more efficient in labeling neurons and glia. Methods CNS neurons and glial cells were labeled with DiI diolistic assay in fixed tissue and living brain slices of C57/B6J mice. Results The method allowed the visualization of the fine structure of neurons and glia including synaptic structures such as dendritic spines. Conclusion With the method, the labeling efficacy of cell's fine structure is improved, making it preferable for the analysis of dendritic spine. In addition, the ability to label the living neuron and glia will extend its application vastly.

0.05).②The mean of SV, the mean of CO and the mean of CI in acute SAH group were lower than those in healthy persons group respectively (33.46?11.33 vs 52.67?12.46,P

Objective To study on comparison of different age group patients with acute abdomen who received laboratory check-ups.Methods 126 patients with acute abdomen were divided into 0.05).2.Of 126 patients,65 cases(51.6%)received abdominal B ultrasonography check-up,among which 41 cases(44.1%)were in

Object To study the chemical constituents from the stem of Photinia parvifolia (Pritz.) Schneid. Methods The compounds were isolated by silica gel column chromatography and their structures were elucidated by means of spectral analysis. Results Seven compounds were identified as lupeol (Ⅰ), ?-sitosterol (Ⅱ), betulinic acid (Ⅲ), pyracrenic acid (Ⅳ), epicatechin (Ⅴ), daucosterin (Ⅵ), pruasin (Ⅶ). Conclusion Compounds Ⅰ, Ⅲ-Ⅵ are isolated from the plants of Photinia Lindl. and compounds Ⅱ and Ⅶ are isolated from P. parvifolia for the first time. Compound Ⅳ shows anticancer effect.

The methods and experiences of anatomy,histology and embryology teaching in English for foreign students are discussed in this article to exchange experiences with each other and progress together.

Aim To evaluate the effect of expression inhibition of spermine oxidase(SMO)on the actitumor activity of polyamine analogue CPENSpm (N~1-cyclopropylmethyl-N~(11)-ethylnorspermine).Methods siRNA technique was used to inhibit expression of SMO in human lung cancer line A549.QT-RT-PCR and enzyme activity assay was performed to determine the expression level of SMO.The cell proliferation was detected by MTT assay.The apoptosis of A549 cells were evaluated by DNA degradation and Sub-G_1/flow cytometry assay.Results The A549 cell line with silenced SMO expression was successfully obtained.Basic SMO mRNA and enzyme activity levels in the SMO-siRNA plasmid transfected cells were 0.53% and 14% lower than that in the control cells respectively. Treating A549 control cells by 10 μmol·L~(-1) CPENSpm for 24 hours resulted in a 10-folds up-regulation of SMO in mRNA level and 20-fold increase in enzyme activity,but this drug-induced SMO expression was obviously prevented in SMO-siRNA plasmid transfected cells.MTT assay demonstrated that SMO expression inhibition decreased the sensitivity of A549 cells to CPENSpm exposure(0～20 μmol·L~(-1)).DNA degradation and sub-G_1 assay proved a deceased ability of CPENSpm to induce apoptosis in SMO-siRNA plasmid transfected cells.Conclusion Up-regulation of SMO by CPENSpm is possibly one of the molecular basics for its antitumor activity.

BACKGROUND: Recent studies have reported that more and more methods were used to prevent and cure tendon adhesion following tendon rupture by repairing tendinous sheath. Especially, amnion membrane is commonly used to effective prevent and cure adhesion and promote healing of biomembrane; however, the effect on tendon adhesion needs to be further studied. OBJECTIVE: To verify the efficacy of amnion membrane preserved in Honghua injection on preventing and curing tendon adhesion following transplanting into foot flexor tendon. METHODS: Bilateral foot flexor tendons of 32 healthy mature chickens were cut off. By anastomosis, amnion membrane preserved in Honghua injection was transplanted into left foot flexor tendon, considering as experimental group. Right foot flexor tendons were randomly divided into two groups: blank control group, anastomosis was performed alone; positive control group, amnion membrane not preserved in any injections was transplanted. At 4 weeks after fixation by plaster cast, sliding function of tendon was detected using biomechanics, and local samples were obtained for histopathological observation. RESULTS AND CONCLUSION: In the experimental group, broken end of left tendon was well healed; fiber tissues were formed surrounding tendon; tissue adhesion was not observed surrounding tendon. Proliferative quantity and adhesion of fiber tissues, as well as content of hydroxyproline in the experimental group were significantly less than in the blank control and positive control groups (P < 0.05, P < 0.01); total inflexion angle of articulationes digitorum pedis and slipping distance of flexor digitorum profundus tendon in the experimental group were significantly greater than in the blank control and positive control groups (P < 0.05, P < 0.01). The results indicated that amnion membrane preserved in Honghua injection might prevent tendon adhesion and effectively promote tendon healing.