Objective:To study changes of ECG and cardiac enzyme levels in patients with arrhythmia after in‐hospital infection .Methods :A total of 71 patients with arrhythmia complicated in‐hospital infection ,who were treated in our hospital ,were selected .ECG and cardiac enzyme levels were measured in all patients at hospitalization and after in‐hospital infection .ECG and cardiac enzyme levels were observed and compared between before and after infec‐tion .Results:Compared with before infection ,after infection ,there were significant rise in percentages of sinus tachycardia (29.58% vs .50.70% ) ,bundle branch block (22.54% vs .40.85% ) ,premature ventricular contraction (57.75% vs .78.87% ) and premature atrial contraction (70.42% vs .95.77% ) , P<0.05 or <0.01 ;significant in‐crease in percentages of ST‐T changes (19.72% vs .94.37% ) ,abnormal ST segment (35.21% vs .69.01% ) and ab‐normal PR interval (71.83% vs .94.37% ) , P<0.01 all;and significant rise in levels of creatine kinase isoenzyme [ (29.66 ± 7.54) U/L vs .(44.68 ± 8.93) U/L] and creatine kinase [ (283.65 ± 36.84) U/L vs .(329.47 ± 35.56) U/L] , P<0.01 both .Conclusion:After in‐hospital infection ,the percentages of arrhythmias and abnormal change of ECG wave and cardiac enzyme levels significantly rise in patients with arrhythmia ,physicians should strive to pre‐vent in‐hospital infection ,the patient's condition aggravation .

Objective: To study significance of detection of serum levels of high sensitive C reactive protein (hsCRP), tumor necrosis factor-α (TNF-α) and interleukin-8 (IL-8) for assessing patient’s condition in patients with coronary heart disease (CHD). Methods: Serum levels of hsCRP, TNF-α and IL-8 were measured in 78 CHD patients undergoing percutaneous coronary intervention (PCI) before and three months after PCI. Among CHD patients, there were 17 patients with acute myocardial infarction (AMI), 36 patients with unstable angina pectoris (UAP) and 25 patients with stable angina pectoris (SAP). Their levels of above indicators were compared with those of 47 healthy subjects (normal control group). Results: （1）Compared with normal control group, there were significant increase in serum levels of hsCRP [ (1.96±0.60) mg/L vs. (22.43±9.68) mg/L, (18.27±8.56) mg/L], TNF-α [ (11.26±3.82) ng/L vs. (60.12±19.37) ng/L, (40.33±15.48) ng/L] and IL-8 [ (48.26±20.87) ng/L vs. (120.36±33.32) ng/L, (105.92±34.2) ng/L] in AMI group and UAP group before treatment (P<0.01 all), and Pearson linear correlation analysis showed that hsCRP was positively correlated with levels of TNF-α and IL-8 (r=0.873~0.956, P<0.01 all); （2）Serum level of hsCRP in SAP group [(6.04±2.38) mg/L] was significantly higher than that of normal control group (P<0.01); （3）Compared with before PCI, there were significant decrease in serum levels of hsCRP[(13.89±6.13) mg/L vs. (2.06±1.42) mg/L], TNF-α[(38.26±14.27) ng/L vs. (13.76±4.12) ng/L] and IL-8[(98.96±32.9) ng/L vs. (50.12±19.85) ng/L] in CHD patients three-month after PCI (P<0.01 all), and they were no significant difference compared with normal control group (P>0.05 all). Conclusions: Detections of serum levels of hsCRP, TNF-α and IL-8 in CHD patients are of certain significance for diagnosis, treatment and prognosis prediction of CHD.

Objective To determine the prevalence of pain beyond the osteoporotic vertebral com-pression fracture (OVCF) in patients undergoing percutaneous vertebral augmentation and the response of pain to the treatment. Methods A retrospective study of 104 patients undergoing percutaneous vertebral augmentation (pereutaneous vertebroplasty and pereutaneous kyphoplasty) was performed to examine the lo-cations and severities of pain beyond the OVCF before and after operation. The Visual Analog Sscale (VAS,10-point scale) and Activities of Daily Living (ADL, 5-point scale) were used. Results Of the 104 patients, 48 (46.2%) suffered pain beyond the OVCF. The pain was firstly located in the sacrococeygeal region and buttocks (26.0%), secondly in the abdomen and sub-xiphoid process (10.6%) and thirdly in the flank, ribs and chest (9.6%) . For all the cases, the mean VAS score decreased from 8.9±0. 8 before vertebral augmentation to 2.9±1.4 after augmentation (t=37.410, P=0.000), and the mean ADL score decreased from 4.1±0.6 before operation to 2.0±0.7 after operation (t=25.331, P =0.000) . For the patients suffering pain beyond the OVCF, the mean VAS score decreased from 8.8±0.9 before vertebral augmentation to 2.8±1.5 after augmentation (t=23.722, P=0.000), and the mean ADL score decreased from 4.2±0.7 before operation to 2.0±0. 7 after operation (t=15.373, P=0.000). The decreases in VAS and ADL scores were not affected by the pain. Conclusion The pereutaneous vertebral augmentation can effectively relieve the pain beyond the OVCF.

Objective To investigate the changes and clinical significance of QT dispersion of patients with acute myocardial infarction especially those after thrombolitic therapy. Methods QTd, QTcd, QTLcd of 56 patients with AMI and 54 healthy people were measure through computer ECG. Results (1) The indexes of QTd in AMI group were significantly longer than normal ( P

Objective To explore the effect of Xiao Ban Xu, a compound Chinese traditional medicinal prescription, on the expression of collagenⅠand Ⅲ of keloid fibroblasts in vitro. Methods Six samples of keloid fibroblasts (KFB) and 6 samples of normal fibroblasts (NFB) entered the study as experimental and control groups, respectively. With the application of Xiao Ban Xu (10?g/ml), the fibroblast culture system and the ABC immunocytochemical staining method were used to investigate the expression of collagenⅠand Ⅲ of KFB and NFB. Results The density of the staining of collagenⅠand Ⅲ in the experimental group was much higher than that in the control group, with (7675 4?825 5 vs 2305 2?320 4 and 10595 2?1311 5 vs 2434 8?356 9; t =13 37 and 12 66, P =0 00004 and 0 00005) or without (11113 1?1304 9 vs 3519 6?236 0 and 11157 7?1300 3 vs 2626 5?426 3; t =14 42 and 13 47, P =0 00003 and 0 00004) the addition of Xiao Ban Xu. Compared with blank control group, the density of the staining of collagenⅠand Ⅲ was degenerated greatly in the experimental group after 48 hours of application of Xiao Ban Xu (7675 4?825 5 vs 11113 1?1304 9 and 10595 2?1311 5 vs 11157 7?1300 3; t =10 31 and 4 68, P =0 0001 and 0 0054). Furthermore, the inhibition ratios of collagenⅠwas 30 7%, and the one of collagen Ⅲ 5 1%, in the experimental group. Conclusions The expression of collagenⅠand Ⅲ of keloid fibroblasts in vitro may be significantly more intensive than the one of normal skin fibroblasts. It seems that the expression of collagenⅠand Ⅲ, especially collagenⅠ, in keloid fibroblasts and normal skin fibroblasts in vitro may be suppressed greatly by the application of Xiao Ban Xu.

Objective To discuss the effect of small needle knife therapy on collagensⅠ and Ⅲ in the hypertrophic scar tissues that was subcutaneously transplanted into nude mice. Methods Six samples of human hypertrophic scar tissues without cuticle were subcutaneously transplanted into the back of 24 nude mice to create the animal models of hypertrophic scar. Ten days after of the operation,the mice were divided into control,0.1 mg/ml triamcinolone,0.2 mg/ml triamcinolone,and small needle-knife groups with 6 mice in each. Specimens of the scar tissues were collected in 14 days for immunohistochemistry to detect the collagen Ⅰ and Ⅲ. Results In all the groups,collagens Ⅰand Ⅲ distributed extensively in the cytoplasm of fibroblast and tissues. Image analysis showed that the concentration of collagens Ⅰand Ⅲ in 0.1 mg/ml and 0.2 mg/ml triamcinolone groups were significantly lower than those in the control (0.09?0.03,0.11?0.05 and 0.12?0.02,0.11?0.01 vs. 0.17?0.04,0.19?0.03,P0.05). Conclusions Small needle-knife therapy can reduce the concentration of collagens Ⅰand Ⅲ in transplanted hypertrophic scar tissues in nude mice.

AIM: To investigate the effects of oral administration of Astragalus membranceus, mung bean and arsenolite on the toxic of the arsenolite induced rats and the possible mechanisms with metallothionein (MT). METHODS: All the rats were oral administration with arsenolite. The Astragalus membranceus and mung bean were compared with the cadmium chloride which induced MT synthesis. The contents of MT were determined by cadmium saturation method, the liver mRNA levels for MT 1, MT 2 were detected by RT PCR. The protective effects of renal and liver were observed by testing alanine aminotransferase (ALT), blood urea nitrogen (BUN) and serum creatinine (SCR). RESULTS: The contents of MT were accorded with the mRNA expression of MT 1, MT 2. Arsenolite, Astragalus membranceus and mung bean could induce the synthesis of MT, but the contents of MT which arsenolite induced were trace. The contents of MT significantly increased after oral administration of Astragalus membranceus and mung bean, especially in the Astragalus membranceus group (P

Object To study the chemical constituents from the stem of Photinia parvifolia (Pritz.) Schneid. Methods The compounds were isolated by silica gel column chromatography and their structures were elucidated by means of spectral analysis. Results Seven compounds were identified as lupeol (Ⅰ), ?-sitosterol (Ⅱ), betulinic acid (Ⅲ), pyracrenic acid (Ⅳ), epicatechin (Ⅴ), daucosterin (Ⅵ), pruasin (Ⅶ). Conclusion Compounds Ⅰ, Ⅲ-Ⅵ are isolated from the plants of Photinia Lindl. and compounds Ⅱ and Ⅶ are isolated from P. parvifolia for the first time. Compound Ⅳ shows anticancer effect.

0.05).②The mean of SV, the mean of CO and the mean of CI in acute SAH group were lower than those in healthy persons group respectively (33.46?11.33 vs 52.67?12.46,P

Objective To examine the expression of p44/42 mitogen -activated protein kinase (p44/42MAPK) and Phospho-p44/42MAPK in cicatrix (hy pertropic scars and keloids) and to aim at exploring the role of activated p44/ 42 MAPK pathway in development of cicatrix. Methods In or der to analyse the differences, 10 samples of normal skin (NS), hypertropic scar s (HS) and keloids (K) were collected, and then the extracted cytoplasmic protei ns from each tissue were examined by Western blotting for p44/42 MAPK and Phosph o p44/42MAPK and immunohistochemical staining with specific antibodies was emplo yed to determine that in fibroblasts of K, HS and NS. Results There was no evident difference of p44/42MAPK in the tissues and fibroblas ts between cicatrix and NS, but Phospho-p44/42MAPK was obviously higher in cica trix than that in NS. In cicatrix, there was no evident difference of p44/42MAPK in tissues between HS and K, while in fibroblasts, Phospho-p44/42MAPK in K was much higher than in HS. Conclusion Activation of p44/42MA PK pathways involves in formation of cicatrix.