creased maternal serum adiponectin level may be involved in the pathogenesis of pre-eclampsia and also may contribute to the reduced expression of adiponectin in the omental adipose tissue.

Objective To investigate the clinical efficacy of nimesulide in the treatment of chronic periodontitis patients,and analyze the effect of IL-8 and TNF-αlevels in the gingival sulcus fluid.Methods 88 patients with chronic periodontitis in our hospital between June 2014 to December 2015 were divided into control group and observation group by the random number method, 44 cases in each group, the control group patients were given metronidazole treatment, observation group patients were given nimesulide treatment,two groups therapeutic effect and the incidence of adverse reactions were observed, compared two groups of patients before and after treatment in gingival sulcus fluid IL-8 and TNF-αlevel, compared sulcus bleeding index (SBI), plaque index (PLI) and clinical attachment level (CAL),before and after treatment two weeks and four weeks of probing depth (PD) of two groups.Results The total effective rate of observation group patients was 95.55%, significantly higher than the control group patients’total efficiency (81.82%) (χ2 =4.06, P=0.04), after treatment in the observation group gingival sulcus fluid IL-8 factor and TNF-αlevels were significantly lower than the control group (P<0.05), After 2 weeks and 4 weeks of treatment, PD, SBI and CAL indicators observation group were significantly lower than the control group ( P <0.05 ) , the incidence of adverse reactions of observation group patients was 4.55%, significantly lower than the incidence of adverse reactions in control group patients(22.73%)(χ2 =6.18, P=0.01).Conclusion Nimesulide in the treatment of chronic periodontitis with a highly efficient clinical efficacy, while reducing gastrointestinal side effects, etc., can effectively reduce the gingival sulcus fluid IL-8 and TNF-αlevels, ease clinical symptoms, is a highly effective treatment.

Objective To investigate the value of minimal invasion technique in treatment of breast benign disease.Methods 204 benign breast tumors of 74 women were completely excised by using Mammotome or VACORA.Results 108 masses were confirmed fibroadenoma,66 fibroadenosis,30 adenosis of breast.Postoperative followed up 3～12 months:there were 2 cases of local hematoma,5 cases of subcutaneously hematoma,1 case of recurrence,no infection.Conclusions There ale many advantages using uhrasound-guided minimal invasion technique to treat benign breast disease,including accurate localization,minimal invasion,less bleeding,easy performation,less complications and good cosmetology.

Objective To investigate the circadian blood pressure(BP)rhythm in hypertensive patients with chronic renal insufficiency.Methods Circadian blood pressure(BP)rhythm was studied by ambulatory 24-h monitoring among hypertensive(n=25,group B)patients with stable progression of chronic renal insufficiency,and among patients with essential hypertension(n=28,group A)without renal disease.Results ①The 24-h diastolic BP and nocturnal systolic and diastolic BP of group B were significantly higher than those of group A[(80.9±13.4)mmHg vs[70.3±15.6)mm Hg,(160.2±17.8)mm Hg vs(140.3+25.9)mm Hg,(82.6±16.1)mm Hg vs(68.8±20.2)mm Hg,P＜0.05)].②Blooa pressure variability(BPV)of 24 hour systolic and diastolic BP of group B was higher than group A[(13.5±3.9)mm Hg vs(11.3±2.1)mm Hg and(9.2±1.2)mm Hg vs(8.3±1.8)mm Hg,P＜0.05).BPV of nocturnal systolic and diastolic BP in group B was signitleantly higher than group A [(14.9±3.3)mm Hg vs(9.3±2.1)mm Hg and (9.7±2.4)mm Hg vs(8.0±2.2)mm Hg,P＜0.01)].③The 24-h profile of BP of group A showed more non-dipper according for 64.3%(18/28),reverse-dipper according for 10.7%(3/28),but which was 40.O%(10/25)and 48.0%(12/25).Conclusion The renal patients show a disturbance in the 24-h profile of BP,higher indexes of noetttrnal BP and a greater nocturnal variability,which may be important factor for event of cardiovascular disease and renal insufficiency.

Objective: To investigate the mortality of malignant tumors, diabetes, cardio-cerebrovascular diseases and chronic respiratory diseases and trends in probability of premature mortality due to these four chronic diseases in Jiangmen City, Guangdong Province from 2012 to 2021, so as to provide the evidence for perfecting the chronic disease control strategy. Methods: The mortality of malignant tumors, diabetes, cardio-cerebrovascular diseases and chronic respiratory diseases among household registered residents in Jiangmen City from 2012 to 2021 were collected from the Guangdong Provincial Population Death Information Registration Management System, and the crude mortality, standardized mortality by the population of the Fifth National Population Census in China in 2000 and probability of premature mortality were calculated. The trends in mortality and probability of premature mortality were analyzed using average annual percent change (AAPC), and whether achieving the targets for the probability of premature mortality due to four chronic diseases in 2025 and 2030 were evaluated. Results: A total of 226 012 deaths occurred due to four chronic diseases in Jiangmen City from 2012 to 2021, and the overall crude mortality and standardized mortality rates were 569.22/105 and 283.29/105, with a reduction in the probability of premature mortality from 15.04% to 12.05% (AAPC=-2.403%, Z=-7.603, P<0.001). The probability of premature mortality due to four chronic diseases decreased from 19.21% to 16.38% in males, and from 10.42% to 7.58% in females (AAPC=-1.893% and -3.085%, Z=-5.262 and -9.811, both P<0.001). The probability of premature mortality due to diabetes appeared a tendency towards a rise (AAPC=2.317%, Z=2.548, P=0.034), and the probability of premature mortality due to cardio-cerebrovascular diseases showed a tendency towards a decline (AAPC=-4.826%, Z=-13.590, P<0.001), while no significant changing trend was seen in the probability of premature mortality due to malignant tumors or chronic respiratory diseases (AAPC=-0.751% and -2.461%, Z=-1.532 and -1.730, P=0.125 and 0.122). The predicted probability of premature mortality due to four chronic diseases was 10.92% in 2025 and 9.66% in 2030 in Jiangmen City, which were both lower than the target (11.21% and 9.81%). Conclusions The probability of premature mortality due to four chronic diseases appeared a tendency towards a decline in Jiangmen City from 2012 to 2021, which can reach the target in 2025 and 2030. Males should be given a high priority for interventions of chronic diseases, and diabetes control should be reinforced.

Objective To explore the impact of the systematic social skills training on recovery of chronic schizophrenia patients with negative symptom and social skills defects.Methods A total of 82 cases of long -term hospitalized patients with chronic schizophrenia were randomly divided into intervention group and control group by digital table,41 cases in each group.The two groups received psychiatric routine therapy and nursing,and general entertainment therapy,and systemic social skills training was conducted in the intervention group for 12 weeks.PANSS and SSC were respectively used to evaluate the two groups before and after the intervention.Results After 12 weeks of systematic social skills training,the total score of SSC[(24.5 ±4.5)points]in the intervention group was apparently lower than that in the control group[(29.2 ±5.2)points],and the difference was statistically significant(t =3.212, P <0.01).Compared with the control group[(20.5 ±4.3)points,(27.2 ±4.2)points,(59.1 ±9.5)points],in the intervention group the score of negative symptom mental pathology and total score of PANSS were (14.9 ± 3.7)points,(24.5 ±3.5)points,(52.8 ±7.1)points.The differences of total score between the two groups were sta-tistically significant(t =5.199,3.317,3.239,all P <0.01),while the positive symptoms score was not statistically significant(P >0.05).Compared with before intervention,in the negative symptom table,the differences of total score N2,N3,N4,N6 had statistically significant differences(t =4.533,5.413,4.501,4.668,all P <0.01).Conclusion The systematic social skills training can greatly promote chronic schizophrenia patients with negative symptom and social skills defects,improve their social skills and life qualities.

Objective To investigate the acute toxicity and anti-stress effects of Naoxinshu capsule (NC) in mice.Methods In acute toxicity experiment,mice were oral administered to determine the maximum tolerance dose of NC.In anti-stress experiments,mice were divided into 5 groups at random,namely NC high,medium and low dose groups (2.4,1.2 and 0.6 g/kg,respectively),Gypenosides group(0.75 g/kg) and control group (n=10).After seven days of oral administration,rope climbing time,loaded swimming time,anti-hypoxia time,and survival rate under high or low temperature tests were carried out,respectively.Results The maximum tolerance dose of NC was 72 g/kg.NC high,medium and low dose groups significantly prolonged the rope climbing time [is (9.5 ± 3.1) min,(6.0± 1.6) min,(4.1 ± 1.2) min],loaded swimming time [is (28.2± 6.8) min,(25.7± 10.3)min,(22.9± 8.0)min] and anti-hypoxia time (around 17 min),compared to the control group (P＜0.05 or P＜0.01).NC high dose group significantly improved the survival rate of mice (i.e.30％) under high or low temperature (P＜0.05).Conclusion NC shows good anti-stress effects,and no detectable acute toxicity in mice.

Objective To approach the effect of CYP3A5 6986A＞G (rs776746) and ABCB1 3435C＞T (rs104564) on plasma tacrolimus(FK506) dose adjusted blood trough concentrations(C0/D) in kidney transplanted patients.Method Totally 125 renal transplanted recipients were recruited,and gene sequencing method were used to detect the genotype of CYP3A5 6986A＞G and ABCB1 3435C＞ T.FK506 trough concentration of 125 patients was detected by enzyme multiplies immunoassay technique (EMIT) after the surgery over 6 month.The relevance between the ratio of trough concentration/dosage and polymorphisms was analyzed.Result In 125 renal transplanted receipients,the allele frequency of CYP3A5 and ABCB1 genetic variants was 74％ and 34.8％,respectively.After 6 months,the C0/D ration in the homozygous mutant CYP3A5 * 3/* 3 receiptients was significant higher than that in the homozygous wild type * 1/* 3 and heterozygous mutant * 1/* 1 receiptients (P ＜ 0.05).And there was no significant difference of the Co/D ration between CYP3A5 * 1/* 1 and CYP3A5 * 1/* 3 receipients(P＞0.05).The the C0/D ration in homozygous wild type ABCB1 T/T and homozygous mutant T/C receipients was significant higher than that in heterozygous mutant C/C receipients (P＜0.05),and there was no significant difference of the C0/D ration between T/C and T/T receipients (P＞ 0.05).Conclusion The study shows that the the polymorphism of CYP3A5 and ABCB1 genes was significantly correlated with the trough C0/D value of the recipients after renal transplantation who were treated by Tacrolimus for more than half a year.The detection of the SNP of the CYP3A5 and ABCB1 will be useful for FK506 dosage adjustment.

Objective To evaluate the quality of Psoralea corylifolia collected from 12 provinces of China.Methods An HPLCDAD-MS/MS method was used to identify,determine,and estimate 14 representative bioactive compounds in P.corylifolia.Then on the basis of the content data,the chemometrics method was used to differentiate 20 samples from different regions.Results The quality of P.corylifolia from 12 different provinces of China was evaluated by this method.Though the samples showed similar profiles,content of the detected markers varied significantly in different regions and batches.According to the results of the hierarchical cluster analysis and principle component analysis,it can be concluded that the samples from different origins could be clustered reasonably into two groups,as well as successfully distinguished.Conclusion A simple and reliable new method which used HPLC-DAD-MS/MS and chemometrics has been developed to characterize,classify,and control the quality of P.corylifolia.

This study aimed at evaluating the effect of different processing methods on the contents of antiosteoporotic compositions in P.corylifolia.HPLC method adopted to analyze the contents of antiosteoporotic compositions,including coumarin (psoralen and isopsoralen),flavonoids (neobavaisoflavone,corylifolin,corylifolinin and corylifol A) and meroterpenes (bakuchiol) in P.corylifolia.The contents were analyzed before and after the processing methods.Results:The results showed that the content of coumarin,flavonoids and meroterpenes obviously changed in different processed P.Corylifolia in comparison with the crude drugs.In conclusion,it was demonstrated that the therapeutic efficacy of osteoporosis was enhanced by CP method which increased all of the three compositions.The testimony tallied with the Chinese medical theory:stir-frying with salt-water could enhance the effect of the tonification of spleen and kidney.