Objective To investigate the effects of the milrinone in adjuvant treatment of intractable heart failure.Methods 60 cases of patients with intractable heart failure were randomly divided into observation group and control group according to hospitalization sequence,30 cases each group.The control group was given conventional therapy for heart failure,the observation group based on the use of milrinone.Changes of cardiac function index,NT-proBNP were compared between two groups before and after medication,and recorded the adverse reactions and the efficacy evaluation.Results After taking drugs,HR,LVEDD of the two groups were significantly decreased(t =5.31,5.92,4.61,4.22,all P ＜ 0.05),SV,LVEF and 6 minutes walking distance increased significantly(t =7.22,4.78,8.78,4.56,4.11,4.05,all P ＜ 0.05) ; group HR,LVEDD were significantly lower than those in the control group(t =4.31,4.92,all P ＜0.05).SV,LVEF and 6-minute walking distance was significantly higher than that in the control group(t =4.31,4.92,4.11,all P ＜ 0.05).After taking drugs,the observation group NT-proBNP levels decreased significantly than before treatment (t =14.55,P ＜ 0.05),and significantly lower than that in the control group (t =7.12,P ＜ 0.05).No significant change in NT-proBNP levels before and after treatment in control group was found (t =1.03,P ＞ 0.05).The observation group in the total effective rate was 93.33 ％ (28/30),the control group was 63.33％ (19/30),the difference was statistically significant(x2 =5.26,P ＜ 0.05).5 cases of adverse reactions to the observation group,4 patients in the control group,no significant difference in the adverse reactions was found between the two groups (x2 =0.67,P ＞ 0.05).Conclusion Low dose,short-term use of milrinone can make the heart function of patients with refractory heart failure was obviously improved,significantly improve the efficacy with higher safety.

Mastering peripheral nerve block technique is a basic requirement for residents stan-dardization training. Ultrasound-guided nerve block has broken through the limitation of traditional blind operation, promoted the quality and safety of anesthesia, and improved the quality of the peripheral nerve block teaching. However, the safety of ultrasound-guided nerve block technique must be based on the speci-fication training. In the teaching experience, we have used theoretical study, simulation training and clinical practice to explore a new method for upgrading the quality of resident doctor training.

Objective To discuss the efficacy and safety of sitagliptin combined with metformin in treatment of type 2 diabetes mellitus(T2DM).Methods One hundred patients with T2DM were divided into two groups by random digits table method with 50 cases each.The patients in study group were given phosphate sitagliptin (100 mg,day 1 oral) combined with metformin (500 mg,day 3 oral).The patients in control group were given repaglinide (1 mg,day 3 oral) combined with metformin (500 mg,day 3 oral).They were treated for 12 weeks and fasting plasma glucose(FPG),2 h postprandial blood glucose(2 h PG),glycosylated hemoglobin (HbA1c),fasting insulin (FINS),body mass index (BMI) and the incidence of hypoglycemic events were detected before and after treatment.Results After treatment,the level of FPG,2 h PG,HbA1c was significantly lower than that before treatment in two groups,and there was significant difference (P ＜ 0.01).The level of B MI after treatment was significantly decreased [(24.45 ± 2.13) kg/m2 vs.(25.46 ±2.62) kg/m2] and FINS was significantly increased [(13.36 ± 1.89) mU/L vs.(12.36 ± 1.80)mU/L] compared with that before treatment in study group (P ＜ 0.05 or ＜ 0.01).But the level of BMI and FINS had no significant difference before and after treatment in control group (P＞ 0.05).The incidence of hypoglycemic events had significant difference between study group and control group [0 vs.12％ (6/50)] (P ＜ 0.05).Conclusions Sitagliptin combined with metformin is safe and effective in treatment of type 2 diabetes mellitus,which is the preferred solution treatment.The risk of hypoglycaemia events is obviously reduced,also it can control and reduce weight and other advantages.

Objective To compare the clinical effect of UK and rt-PA intravenous thrombolytic therapy for acute myocardial infarction.Methods By SAS statistical analysis system,72 patients with acute myocardial infarction were randomly divided into rt-PA group and UK group,each group had 36 cases.The coronary artery recanalization rate,bleeding events,adverse cardiovascular events,mortality,and cardiac troponin T(cTnT) level were recorded.Results In rt-PA group,0.5h,1 h,2h after the start of thrombolytic therapy,the coronary artery recanalization rate was significantly higher than that in the control group,the difference was statistically significant (x2 =7.32,6.12,5.81,all P ＜ 0.05).In rt-PA group,3 cases had bleeding events,2 cases died,and in UK group,5 cases had bleeding events,3 cases died,,there was no statistically significant difference between the two groups (x2 =1.22,P ＞ 0.05).In rt-PA group,there were 6 cases with adverse cardiovascular events,14 cases in the control group,the difference was statistically significant (x2 =4.56,P＜0.05).2 cases died in rt-PA group,3 cases died in UK group,the mortality between the two groups had no statistically significant difference (P ＞ 0.05).The cTnT level in the hospital 6h,12h was significantly higher than admission in two groups (t =6.67,6.30,4.11,4.22,4.40,all P ＜ 0.05).In rt-PA group,cTnT level in the hospital 6h,12h was significantly lower than that in UK group (t =5.92,6.61,all P ＜0.05).Conclusion Compared with UK,rt-PA in the treatment of acute myocardial infarction can increase recanalization rate,decrease adverse events and myocardial injury,it is worthy of clinical application.

ObjectiveTo investigate the effect of sevoflurane anesthesia in the neonatal period on cognitive function and acetylcholinesterase(AChE) activity in hippocampus in developmental stages in rats.Methods Eighty 7 day-old SD rats weighing 12-16 g were randomly divided into 5 groups( n ＝ 16 each):groups A and B inhaled 3 ％ sevoflurane in oxygen for 6 and 2 h respectively; groups C and D inhaled 1.5 ％ sevoflurane in oxygen for 6 and 2 h respectively; group E inhaled oxygen only.The cognitive function was assessed using Morris water maze test in weaning period(16-21 day-old) and sexual maturity period (55-60 day-old) respectively.The rats were sacrificed at 30 min after Morris water maze test was finished at 21 and 60 d after birth,and the hippecampus were removed for determination of AChE activity using colorimetry method.ResultsCompared with group E,escape latency was prolonged during 17-20 d after birth in group A,and at 18,19 d after birth in groups B and C,and at 18 d after birth in group C,and AChE activity increased at 21 d after birth in group A( P < 0.05 or 0.01 ).Compared with group A,escape latency was shortened at 19,20 d after birth,and AChE activity decreased at 21 d after birth in groups B and C( P < 0.05 or 0.01).Compared with groups B and C,escape latency was shortened at 19,20 d after birth in group D( P < 0.05).There were no significantly differences in probe time in original platform quadrant,frequency of crossing the original platform and AChE activity at 60 d after birth and escape latency during sexual maturity period among the 5 groups( P ＞ 0.05).ConclusionSevoflurane anesthesia in the neonatal period can reversible decrease the congnitive function in developmental stages in rats in concentration and time dependent manners,and the mechanism may be related to increasing the AChE activity in hippocampus.

Humans ; Infant, Newborn ; Mastocytosis, Cutaneous

Objective To analyze the change and clinical significance of blood lipid and high sensitive-C reactive protein (hs-CRP) in patients with subclinical hypothyroidism.Methods Fifty patients with subclinical hypothyroidism were selected as observation group,and 50 health people with the same gender and age were selected as control group.The blood lipid and hs-CRP levels in the two groups were measured and compared.Results The total cholesterol (TC),triacylglycerol (TG),and low density lipoprotein cholesterol (LDL-C) in observation group were significantly higher than those in control group [(5.20 ±0.84) mmol/L vs.(4.79 ±0.80) mmol/L,(2.21 ±0.97) mmol/L vs.(1.78 ±0.60) mmol/L,(3.25 ±0.65) mmol/L vs.(2.83 ±0.51) mmol/L],high density lipoprotein cholesterol (HDL-C) was significantly lower than that in control group [(1.18 ± 0.15) mmol/L vs.(1.28± 0.22) mmol/L],and there were statistical differences (P ＜ 0.05 or ＜ 0.01).The hs-CPR in observation group was significantly higher than that in control group [(4.2 ± 1.1) mg/L vs.(3.0 ± 0.8) mg/L],and there was statistical difference (P ＜0.01).Conclusions Subclinical hypothyroidism can cause increased TC,TG,LDL-C and hs-CRP and decreased HDL-C,which may accelerate atherosclerosis and cardiovascular disease.It is necessary to timely screen and treat the disease.

Objective To study the risk factors for cerebral infarction resulting from transient ischemic attack (TIA).Methods The clinical data of 144 hospitalized patients with TIA were evaluated.Results 50 patients (34.7%) suffered from cerebral infarction(CI) following the onset of TIA in 144 patients.11 cases with CI resulted from vertebra-basilar artery system,37 cases resulted from internal carotid artery system. There was significantly different in two artery systems( P

Objective To investigate and report the catalogue and distribution of acaroid mites in region of Jianghuai.Methods The dust samples were collected from storage circumstances, working places and human dwellings from Jianghuai region(11 cities) in Anhui province from April to October, 2006.Acaroid mites were isolated by waterflotation and tullgren, then counted and identified.Results Thirty species of acaroid mites were identified, belonging to 20 genera, 7 families(4 subfamilies), the dominant species were Tyrophagus putrescentiae, Acarus siro, Lepidoglyphus destructor, Glycyphagus domesticus, Dermatophagoides farinae and Dermatophagoides pteronyssinus.Conclusion Acaroid mites breeding in differenai habitats of Jianghui region distributes widely and shows significant diversity.

Objective:To investigate the effect of Shuanghuangbu slow releasing strip (SRSS) on the expression of BMP2 and IGF1 in the experimental regeneration of periodontium. Methods:Periodontal defects were surgically made around the second incisor and the ca nine in four beagle dogs .32 defects were randomly divided into experimental gr oup and control group with 16 defects in each group. SRSS was applied to the e xperimental group and no disposition to periodontal defect in the control group. Two teeth of each dog were randomly chosen as the natural controls.Specimens we re respectively obtained one month and three months after operation. BMP2 and I GF1 were quantitatively analyzed by immunohistochemistry. Results:The positive rate of BMP2 and IGF1 in experimental group were higher t han those in the control( P