OBJECTIVE: To study the change of the active components in Radix Paeoniae Rubra before and after the radiation of 60Co-?-rays.METHODS: The content of paeoniflorin in Radix Paeoniae Rubra before and after the radiation of 60Co-?-rays was determined by HPLC on Spherigel ODS C18(150 mm?4.6 mm,5 ?m) chromatographic column.The mobile phase consisted of methanol-water(gradient elution) with the detection wavelength set at 260.5 nm.RESULTS: The content of paeoniflorin in Radix Paeoniae Rubra dropped by nearly 20% after the radiation of 60Co-?-rays.CONCLUSION: The effect of the radiation of 60Co?-rays on the active component should be taken into consideration in treating the Chinese drugs preparation of Radix Paeoniae Rubra.

Aim To investigate the effects and mechanisms of DMA on cardiac function in cardiomegaly rat isolated hearts.Methods With Langendorff perfusion,the changes in the indicators reflecting cardiac function were observed in cardiomegaly rat isolated hearts.Its probable mechanism was explored with calcium channel blocker and sodium calcium exchange(NCX) blockers.Results DMA(0.5～2 ?mol?L~(-1))enchanced cardiac function and exerted positive effects on LVSP-LVDP,+dp/dt_(max)and-dp/dt_(max) in Langendorff-perfused cardiomegaly rat isolated hearts.The effects of DMA on cardiac function in cardiomegaly rat isolated hearts weren′t blocked by nicardipine-one of calcium channel blockers and were blocked by NiCl_2——one of sodium calcium exchange(NCX) blockers.Conclusion DMA can enhance systolic and diastolic function in cardiomegaly rat isolated hearts.By agitating NCX,DMA enhances cardiac function in cardiomegaly rat isolated hearts,independent of L-calcium channel.

15℃ . Conclusion The changes of density of different strains of Escherichia coli in the undernourished water samples closely associated with the strain ' characteristics, environment temperatures and preserving time, showed significantly differences.

OBJECTIVE To search an applicable software in order to achieve real time monitoring and processing management for nosocomial infection which may improve the medical service efficiently through comparing with three different methods.METHODS To analyze and compare the three surveillance methods:the manual summed-up statistical methods,the National Computer Management System for Nosocomial Infection Surveillance Network and the Beijing Information System for Nosocomial Infection Surveillance and Control(Beijing System).RESULTS Beijing System is much more pragmatic and multipurpose than the two others.CONCLUSIONS Beijing System has the feature of wide coverage and convenience manipulating.It is confirmed practical and universal.And it is superexcellent and available for different kinds of hospitals.So Beijing System is worth for being popularized.

Objective To investigate the effect of ginsenoside-Rgl on paraurethral fascia fibroblastsmultiplication and the expression of proliferation cell nuclear antigen (PCNA) of stress urinary incontinence(SUI) women in vitro. Methods Specimens of human paraurethral fascia were obtained from 4 SUI womenduring tension-free vaginal tape (TVT) or tension-free vaginal tape-obturator (TVT-O) procedure.Fibroblasts were isolated and cultured by outgrowth technique. After reaching confluency fibroblasts weresubcultured every 5 days and cells after passage number 3 to 5 were used for assessment. The paraurethralfascia fibroblasts were treated with ginsenoside-Rgl at different concentrations (5, 10, 20 μmol/L) andfibroblnsts without Rgl were used as controL The multiplication conditions of paraurethral fascia fibroblastswere respectively detected by methyl thiazolyl tetrazolium (MTr) assay and the expression of PCNA byhistochemistry. Results ( 1 ) Compared with the control group, the growth rate of cells treated with differentconcentrations of Rgl after 72h [ (29±5 )%, (40±5 )%, (26±4)% respectively ] was significantly higher(P<0.01). (2)Compared with the control group, the stimulatory effect of Rgl on fibroblast growth wassignificant at 24 h (P<0.01), and peaked at 72 hi (29±5)% ,(40±5)%, (26±4)% respectively, P<0.01]. (3)Compared with the control group(28.77% ), there was a significant increase of PCNA-positivecells (P<0.01) after 48 h treatment with different concentrations of Rgl (49.24%, 83.48%, 54.50%respectively). Conclusion The results indicate that, at least in vitro, fibroblasts from paraurethral fasciataken from women suffering from SUI are able to proliferate after

OBJECTIVE:To investigate therapeutic efficacy and recurrence rate of vaginal administration of lactobacillus com-bined with chlorquinaldol-promestriene for senile patients with atrophic vaginitis. METHODS:150 senile patients with atrophic vag-initis were selected and divided into group A,B,C according to random number table,with 50 cases in each group. Group A was given Lactobacillus vaginal capsules,2 capsules,qd (at bedtime);group B given Chlorquinaldol-promestriene vaginal tablets,1 tablet,qd(at bedtime);group C given Chlorquinaldol-promestriene vaginal tablets,1 tablet,qd(at bedtime),and Lactobacillus vaginal capsules,2 capsules,qd,next morning,implanting into vagina. 3 groups received treatment for 18 d. Clinical efficacy, vaginal health score,vaginal symptom score and vaginal pH value were observed in 3 groups,and the occurrence of ADR and re-currence rate during follow-up period (12 months) were recorded. RESULTS:The total effective rate of group C was 94.00%, which was significantly higher than group A(72.00%)and B(74.00%),with statistical significance(P<0.05). Before treatment, there was no statistical significance in vaginal health score,vaginal symptom score and vaginal pH value among 3 groups (P>0.05). After treatment,vaginal health score of 3 groups were increased significantly,while vaginal symptom score and vaginal pH value were decreased significantly;and the improvement of above indexes in group C were significantly better than in group A and B,with statistical significance(P<0.05). No obvious ADR was found in 3 groups during treatment. The recurrence rate of group C during follow-up period(4.00%)was significantly lower than that of group A(18.00%)and group B(20.00%),with statistical significance(P<0.05). CONCLUSIONS:Vaginal administration of lactobacillus combined with chlorquinaldol-promestriene for se-nile patients with atrophic vaginitis can effectively relieve clinical symptoms and signs,improve vaginal cleanliness,regulate the lo-cal pH value and reduce the risk of recurrence,with good safety.

OBJECTIVE:To observe clinical efficacy of salmeterol and fluticasone propinate inhalation in the treatment of chronic obstructive pulmonary disease (COPD).METHODS:348 patients were randomly diuided into trial group(n=180) and control group(n=168).Both groups received comprehensive treatment for 15 days.Trial group additionally received one time of seretide inhalation twice a day.RESULTS:The clinical symptoms and the improvement of lung function in trial group were superior to in control group obviously.CONCLUSION:Salmeterol and fluticasone propinate inhalation can be applicable to treat COPD and have an ideal assistant effect.

Purpose To observe the effect of MK on proliferation, migration and angiogenesis of human umbilical vein endothelial HU-VECs cells and to explore the role of MK in tumor angiogenesis. Methods siRNA targeting MK was used to downregulate MK expres-sion in human breast cancer cell line MDA-MB-231. Then, the experiment was divided into three groups: the untreated group, the empty vector transfection group and MK gene interference group. CCK-8 assay was used to detect the endothelial cells proliferation, tr-answell method was for detection of endothelial cell migration numbers, and matrigel in vitro small tube formation assay was used to sur-vey the state of tube formation. Expression of MK in siRNA transfection was identified by RT-PCR and Western blot. Results The MK gene interference group showed lower cell proliferation activity, less number of migration of cells and tube formation than the other two groups (P<0. 05). Conclusion Low-expressed MK in human breast cancer cell line MDA-MB-231 can inhibit proliferation, mi-gration and angiogenesis of endothelial cells, which shows that MK may play an important role in tumor angiogenesis.

Objective To study the relationship between intra-tumor micro vessel density (MVD) clinic opathological features and prognosis of patients with hapatocellular carcinoma(HCC). Methods 30 cases of pathological sections undergone radical surgery for HCC,were evaluated after HE staining and anti-CD 34 monoclonal antibody immuno-histochemical staining. The number of endothelia cells stained by anti-CD34 antibody was counted. The relationship between MVD and survival rate afte roperation was evaluated. Results The surviva Irate was significantly lower in the tumor of larger size, poor stage and higher MVD than that in the other group(P

Objective To study the relationship between infection and hypokalemic paralysis and its clinical features.Methods A systemic analysis was made on the infection courses,clinical manifestations, serum levels of potassium and creatine phosphokinase,the results of electrophysiological and virological examinations in present 34 patients and 115 cases reported in literature with hypokalemic paralysis.Results There were differences in the clinical manifestations and denominations among the present and reported cases. No definite evidences for virus infections were found among patients who were characterized by acute flaccid paralysis accompanied with hypokalemia in the infection curses. The prognosis of the disorder was better after the patients were given potassium, and most of them didn't recur.Conclusion No enough evidences showed that this disorder was an independent disease.It should be named as "concomitant infectious hypokalemic paralysis" appropriately. It was probably a syndrome accompanied by infection on the base of hereditary susceptibility.