Minimal residual disease (MRD) is the cause of relapse in leukemia,and its dynamic monitoring has been a hotspot yet proven to be difficult.MRD in acute B-lineage lymphoblastic leukemia has its own characteristics,normal bone marrow produces a large number of B precursor cells,easily mistaken for leukemia cells,and there were no specific gene alterations that can be conveniently detected.This paper summarizes the antibody combinations in flow cytometry,timing for surveillance and polymerase chain reaction for gene detection,and provide a reference for B lymphocytic leukemia MRD monitoring.

Objective To investigate the effects of alprostadil injection on IL-6, IL-8, TNF-αand insulin resistance in diabetic patients.Methods 90 patients with diabetes mellitus in our hospital from January 2014 to May 2016 were selected,and randomly divided into observation group and control group,45 cases in each groups.The control group received conventional western medicine , the observation group was treated with alprostadil injection.The levels of serum IL-6, IL-8, TNF-α, insulin and insulin resistance index were compared before and after treatment in both two groups. Results After treatment,the total effective rate of treatment group was 91.11%,which was significantly higher than the control group 71.11%( P<0.05).Before treatment,the levels of IL-6,IL-8,TNF-α,insulin and insulin resistance index of two groups were had no statistical significance,after treatment,the levels of IL-6,IL-8,TNF-α,insulin and insulin resistance index were decreased in both groups,and the observation group was significantly lower than the control group,the difference was statistically significant (P<0.05).Conclusion The alprostadil injection can significantly reduce the levels of IL-6,IL-8,TNF-α,and control the level of blood glucose,reduce the effect of insulin resistance.

Objective To investigate current status and influencing factors of patient delay in patients with advanced hepatocellular carcinoma and provide a theoretical evidence for the early diagnosis of HCC. Methods All patients with a primary diagnosis of advanced HCC admitted at Department of Hepatobiliary Medicine I, Eastern Hepatobiliary Surgery Hospital of the Second Military Medical University from January 2015 to October 2015 were investigated prospectively. The current status of patient delay was assessed by medical records and influencing factors of patient delay were analyzed by the self-designed questionnaires. Results Rate of patient delay in the patients with advanced HCC was 27.7%(53/191). The univariate analysis demonstrated that significant influencing factors for patient delay included age, education levels, economic status, place of residence, medical insurance, knowledge about follow-up examination for chronic viral hepatitis. The rate of patient delay was high in these patients with older age, lower income, less education, place of residence (countryside or small towns), new rural insurance, lack of the knowledge about follow-up examination for chronic viral hepatitis. Multivariate analysis showed that age and lack of the knowledge about follow-up examination for chronic viral hepatitis were significantly independent predictors for patient delay. Their OR (odd ratio) and 95%CI (confidence interval) were 2.770 and 1.424-5.388, 7.867 and 1.669-37.092, respectively. According to place of residence, the univariate analysis demonstrated that significant influencing factors for patient delay included marital status, age, economic status, and knowledge about follow-up examination for chronic viral hepatitis in the countryside or small towns. Multivariate analysis also further showed that age and lack of the knowledge about follow-up examination for chronic viral hepatitis were significantly independent predictors for patient delay. Their OR and 95% CI were 3.812 and 1.550-9.373, 15.400 and 1.793-132.245, respectively. Patient delay of HCC patients in county seat and city was not significantly associated with all of above mentioned influencing factors. Conclusions Patient delay of the patients with advanced HCC in countryside or small towns was comparatively common. To early find high risk factors of HCC, government should be intensified screening to chronic hepatitis B, hepatitis C in the middle and elderly population. To reduce the rate of patient delay in advanced HCC and improve results of treatment for advanced HCC, health care workers should popularize medicine-associated knowledge, especially about chronic hepatitis and its complications.

Objective To investigate the attitudes of medical students regarding to organ donation compensation.Method A self administered questionnaire was distributed to 600 medical students selected randomly from two medical universities in China between January 2013 and January 2014.The questionnaire included demographics and core questions related to medical student's attitudes regarding organ donation compensation.Result A total of 600 questionnaires were distributed and 592 valid responses were received,including 150 doctoral students,143 master students and 299 undergraduate students.In all,69.8％ of medical students believed that compensation for organ donor was essential.Nearly 60.5 ％ believed financial compensation would increase the rate of organ donation but would not tarnish the behavior of organ donors (64.7％).At the same time,over 70％ of medical students believed that financial compensation would increase the number of living organ donation.With regarding to the ways of compensation,most of the students (80.4％) were in favor of priority on waiting list.Reducing or free of hospitalization costs (50.4％) and increasing the reimbursement ratio of medical insurance (41.8％) were also the acceptable compensation ways to medical students.Conclusion Most of medical students believe that it is essential to give compensation to organ donors.Enjoying the priority on waiting list,reducing or free of hospitalization costs and increasing the reimbursement ratio of medical insurance are the reasonable incentive methods.

ObjectiveTo get to know the status of health statistics postgraduate teaching and requirements during the study, and provide a basis to improve medical statistics teaching. MethodsPostgraduate students of 2008 grade of Dalian Medical University were selected randomly and filled in the self-designed questionnaire. Results52.6％ of the students considered that medical statistics was more important than other courses. 72.6％ of the students thought that medical statistics was difficult. Up to 30％of the students couldn't master and apply superior statistical analysis technique. ConclusionThe results of the survey offered basis and direction for enhancing the teaching quality and improving teaching methods.

Objective: To discuss the risk factors of suicide with psychological autopsy method. Methods: 66 complete suicides from Jinzhou and Zhuanghe of Dalian , LiaoNing province, which were individually matched with control population. Results: Three main risk factors of suicide remained in the final conditional logistic regression model. They were social support (OR = 0.66, 95%CI = 0.48 - 0.91 ), depression (OR=1.18, 95%CI=1.03-1.35) and neurosis (OR=2594.59,95%CI=6.29-1069550) . Conclusion: The combined effect of multiple factors increases the risk of suicide but not the unique effect of single factor. The suicide preventive efforts should focus on individuals exposed to multiple risk factors.

The enantiomeric resolution of lactic acid was studied by reversed high performance liquid chromatography. β-cyclodextrin (β-CD), dimethyl (DM) and TM-β-CD were used as chiral mobile phase additives. The effects of different mobile phase, pH and concentration of chiral mobile phase additives on resolution of D,L-lactic acid were investigated. The results showed: the enantioselectivity of TM-β-CD was better than those of β-CD and DM-β-CD. D,L-lactic acid could be partly separated by DM-β-CD 　and could not be separated by β-CD, while it could be fully separated by TM-β-CD. As the concentration of TM-β-CD changed from 0.20　mmol/L to 1.00　mmol/L, The resolution varied slightly. The experiment of the effect of pH of mobile phase on Rs showed: as pH changed from 2.4 to 3.0, the Rs remain stable, and when pH＞3.1, the Rs decreased with the increase of pH. The RSD of retention time of L-lactic acid and D-lactic acid were 0.2% and 0.2%, respectively. The chromatographic systems with a dynamically-generated stationary phase with 2,3,6-tri-O-methyl-β-cyclodextrin proved to be an effective method for lactic acid enantiomer separation

In vitro diagnostic ( IVD) manufacturers were important suppliers of external servicesto medical laboratories .There equipment , reagents , calibration and maintenauce services played an important role in ensuring the quality of medical laboratory tests .It is also helpful for IVD manufacturers to establish and maintain a good reputation and credibility .ISO 15189:2012“Medical laboratories -Requirements for quality and competence” was issued onNovember 1, 2012, which replaced the second edition (ISO 15189:2007).Requirements related to IVD manufacturers are involved in the new edition standard .The article aimed to help medical laboratories and IVD manufacturers understand the requirements ; therefore , laboratories would know how to select suppliers based on their ability to supply external services , IVD manufacturers would know how to meet the needs of laboratories , and the cooperation between IVD manufacturers and laboratories would be facilitated .

AIM To observe effects of IBMX on cGMP and GFAP production in gerbil hippocampus after recirculation following ischemia. METHODS Bilateral occlusion of common carotid arteries and immunofluorescent stain methods in gerbil hippocampal tissue slice were used. RESULTS Recirculation following ischemia leads to a rise in hippocampus cGMP and GFAP concentration. cGMP and GFAP positive cells mainly distributed in rediatum layer and molecular layer in the CA 1 subfield, Most of cGMP positive cells are astrocytes under double immunofluorescent stain. Most of cGMP strong positive cells are also those of GFAP strong positive cells. IBMX increased cGMP and GFAP production. CONCLUSION cGMP is possible to play an important role in the activation or regulation of GFAP in the hippocampus.

ISO 15189:2012Medical laboratories-Requirements for quality and competence was issued on November 1,2012,which replaced the second edition (ISO 15189:2007 Medical laboratoriesParticular requirements for quality and competence).A study on changes in new edition standard will facilitate medical laboratories,regulatory authorities,accreditation bodies to have a good understanding of requirements of this new edition.An implementation of new edition will assist medical laboratory to improve their management to continually meet requirements of international standards.To enable stakeholders mentioned above have a good understanding and application of this new edition,changes in new edition were discussed in depth in this article.