OBJECTIVE:To optimize the extracting process of the total flavonoids from Herba Epimedii.METHODS: The extract technology of the total flavonoids from Herba Epimedii was optimized by orthogonal experiment with the yield of total flavonoids from Herba Epimedii as index and with the extraction temperature,extraction time,the ratio of solid to liquid and the concentration of ethanol as factors.RESULTS:The optimum extracting conditions for the total flavonoids from Herba Epimedii were as follows:the reflux extraction was conducted twice(1 hour/time) at 80 ℃ with 60% ethanol as solvent,and the ratio of solid to solution was 1∶10.CONCLUSION:The optimum extracting technology is simple and reasonable,and it serves as a good reference for the extracting process of the total flavonoids from Herba Epimedii.

60 mm and left ventricular ejection fraction (LVEF)

55 mm and LVEF

Objective To investigate the effect of Tongxinluo on VSMC apoptosis and protein expression of Bcl-2 and Bax in rabbits after angioplasty. Methods Atherosclerotic and PTCA models were set up in 60 rabbits which were randomly divided into 3 groups, the control group the hypertipidemia group and the Tongxinluo group. Apoptosis was determined by TUNEL method and protein expressions of Bcl-2 and Bax were detected by S-P immunohistory chemistry staining. Results Apoptosis of VSMC in the hyperlipidemia group was less than that in the control group, but the level of apoptosis in the Tongxinluo group was even higher compared with the hyperlipidemia group. The level of Bcl-2 expression was found higher in the hyperlipodemia group than in the control group. The expression was mainly seen in endothelium and tunica media of the arteries. The expression of Bcl-2 in the Tongxinluo group is the least among the 3 groups studied. Bax expression, which can mainly be seen in the endothelia, is higher in the Tongxinluo group than that in the hyperlipidemia group. Conclusion Tongxinluo may enhance the apoptosis of VSMC, downregulating the expression of Bcl-2 protein and upregulating the expression of Bax protein.

Objective To explore the post-implant complications of permanent cardiac pacemakers,their reason,treatment and preventive measures.Methods The records of 516 patients receiving a permanent pacemaker were reviewed and complications of the patients were analyzed.Results There were 69 cases of complications after the permanent cardiac pacemaker implantation,5 cases(7.2%) of infection,10 cases(14.5%) of haematoma,7 cases(10.1%) of pocket rankle,10 cases(14.5%) of hemothorax and pneumothorax,10cases(14.5%) of pacemaker syndrome,7 cases(10.1%) of undersensing,2 cases(2.9%) of pacemaker mediated tachycardia,3 cases(4.4%) of battery exhaust advance,3 cases(4.4%) of arrhythmia,10cases(14.5%)of electrode displacement and 2 cases (2.9%) of electrode fracture.Conclusion Good prognosis will be resulted from deep understanding of the usual complications,increased differentiating ability, emphasized pre-operation prevention,standardized operation,strengthened follow-up as well as the education of pacemaker knowledge,early discovery and treatment of the complications.

BACKGROUND:Heterogeneous deproteinized bone because of wide source and its special biological characteristics maybe a good bone tissue engineering scaffold.Its immunogenicity and mechanical properties are different by different interventions.OBJECTIVE:To proof the preparation technology,immunological and mechanical properties of heterogeneous deproteinized bone.DESIGN,TIME AND SETTING:A control observational experiment was performed in the Heilongjiang Key Laboratory of Anti-fibrosis Biotherapy from February to October 2008.MATERIALS:Fresh femoral cancellous bones extracted from adult pigs,which were not including cartilages and cortical bones,were prepared into size of 3 cm?0.5 cm?0.5 cm.Their long axis direction was the same with orientation of trabeculae.The apparent pore densities of the used bones were almost same.METHODS:The bones were soaked in chloroform-carbinol(1:1) 24 hours for degrease.They were shaken and washed by distilled water at 50 ℃,and then put into 20% H2O2 for 24 hours.The procedure repeated three times.The bones were soaked in distilled water at room temperature for 24 hours,and then dryed.MAIN OUTCOME MEASURES:The gross morphological and histological features of the deproteinized cancellous bones were observed.The contents of amino acid and their biomechanical properties were measured.RESULTS:The three-dimensional space structures of the deproteinized cancellous bones were not damage greatly,and they had a natural pore network system which composited of hydroxyapatite and collagen.The contents of collagen amino acids in the deproteinized cancellous bones had no obvious difference from fresh cancellous bones,but the wave crests of aromatic amino acids such as tyrosine and methionine were disappear.The elastic moduli of deproteinized cancellous bones were significantly higher than those of fresh cancellous bones(P

80%).Two patients had side lateral vein branches,and 5 patients had side branches of posterolateral veins.There was no significant relationship between the venous variation and sex.The rate of venous variation was higher in patients with noischemic cardiomyopathy than in patients with ischemic cardiomyopathy(91.25% vs 72.72%,P

[Objective]To study the properties of xenogeneic deproteinized bone (DPB) used as scaffold in the bone tissue engineering and its application to the spinal fusion of the lumbar intertransverse process in a goat. [Methods]The deproteinized bone was derived from an adult pig femoral cancellous bone through physical and chemical treatments.The cell-material complex was observed under the inverted phase contrast microscope to evaluate the adhesion and the growth of the osteoblasts. The experimental model of the spinal fusion in the lumbar intertransverse process was produced in 24 male goats aged 6-8 months, which were divided into 3 groups according to different implant graft. All the samples were harvested at 4,8,12 weeks postoperatively, and a series of examinations were performed, including the radiography and the histomorphological assay.[Results]It showed that The DPB maintained natural pore network system, it hardly had any antigen, so it had good histocompatibility. In the spinal fusion model of lumbar intertransverse process,the cell-material complex could form cartilage and had a new bone formation in a multipoint way, the osteogenic process was almost the same as the auto-ilium osteogenesis and had a good mechanical strength.[Conclusion]The xenogeneic deproteinized bone is a good material in the bone tissue engineering, which can be used as an osteogenesis scaffold and provides a stable fusion.

Laboratory animal welfare is an important guarantee for the accuracy and reliability of experimental results, so it has been paid attention in scientific research .But the situation is not optimistic in the teaching practice .This paper has specially analyzed laboratory animal welfare and ethics in experiment teaching and provided specific measures involved in legislation , spread of animal welfare and implementation of 3R principle to ensure animal welfare .

Purpose To observe the effects of midkine ( MK) on human breast cancer cell line MDA-MB-231 angiogenesis in vitro, and to explore its mechanism. Method shRNA interference was performed to silence the expression of MK in MDA-MB-231 cells, and Western blot was used to identify the expression of MK and EPCR. After MK and EPCR knockdown, or treated with anti protease-activated receptor 1 (PAR1) antibody, the culture medium of MDA-MB-231 cells were collected and the conditioned medium were pre-pared. Human umbilical vein endothelial cells ( HUVECs) were cultured with conditioned medium, and the endothelial cells prolifera-tion was detected by CCK-8 assay, cell migration was detected by transwell method, vasculogenic activity was assessed by Matrigel-based tube formation assay. Results After knockdown of MK, the protein level of EPCR was decreased in MDA-MB-231 cells. Com-pared with control, knockdown of MK and EPCR decreased the proliferation, migration and angiogenesis ability of HUVECs significant-ly (P<0. 05), and the effect of EPCR knockdown group was stronger than MK knockdown group (P<0. 05). After treated with anti-PAR1 antibody, the proliferation, migration and angiogenesis ability of HUVECs were decreased compared with control and EPCR knockdown group (P<0. 05). Conclusion MK promotes human breast cancer cell line MDA-MB-231 angiogenesis through EPCR /PAR1 signaling pathway in vitro.