Objective To investigate the effect of Calf Spleen Extractive Injection(CSEI) on immune function of immunocompromised mice. Methods BALB/C mice were divided into normal group (n=20), model group (n=20) and positive control group (n=20), CSEI high dose group (1.20 mL/kg, n=20), middle dose group (0.60 mL/kg, n=20) and low-dose group (0.30 mL/kg, n=20). Besides the normal group, mice in other groups were intraperitoneal injected cyclophosphamide at dose of 80 mg/kg for three consecutive days to induce immunocompromised model. Continuous dosing after 10 days to viscera index, index, natural killer cells (natural killer cell, NK) kill rate, the number of peripheral blood leukocytes (white blood cell, the WBC), lymphocyte count (lymphocytes, LYM) and serum against sheep red blood cells (the Sheep Red blood cell, SRBC) IgG levels as examining index, verify the CSEI effect on the regulation of immune function was low immune function in mice. Results Compared with model group, CSEI groups of high middle and low dose had improved viscera index, phagocytic coefficient, NK cell killing activity, serum specific antibody level, peripheral WBC count and lymphocyte count(P<0.05). Conclusion CSEI has possess immunomodulatory effects on the immunocompromised mice.

Objective To explore the curative effect of the different utilized timing of romethamine in cesarean section with central placenta previa.Methods All of 148 patients with central placenta previa were divided into observation group (74 patients) and control group (74 patients) according to the serial number.The patients in observation group were given romethamine after breaking off umbilical core,the patients in control group were given romethamine after placental expulsion.The intraoperative blood loss,postoperative 24 h blood loss,operation time,rate of automatic placental expulsion and adverse reaction were compared.Results The intraoperative blood loss in observation group was less than that in control group[(503.5 ± 320.8) ml vs.(672.1 ± 409.6) ml],the rate of automatic placental expulsion was higher than that in control group [79.73％ (59/74) vs.54.05％ (40/74)],the operation time was less than that in control group [(42 ± 15) min vs.(55 ± 16) min],there was significant difference (P ＜0.05 or ＜0.01).The postoperative 24 h blood loss and the adverse reaction between two groups had no significant difference (P ＞ 0.05).Conclusions In cesarean section with central placenta previa,given romethamine after breaking off umbilical core can improve the rate of automatic placental expulsion,shorten operation time,decrease intraoperative blood loss.It is worthy of spreading.

Objective To investigate the effects of the milrinone in adjuvant treatment of intractable heart failure.Methods 60 cases of patients with intractable heart failure were randomly divided into observation group and control group according to hospitalization sequence,30 cases each group.The control group was given conventional therapy for heart failure,the observation group based on the use of milrinone.Changes of cardiac function index,NT-proBNP were compared between two groups before and after medication,and recorded the adverse reactions and the efficacy evaluation.Results After taking drugs,HR,LVEDD of the two groups were significantly decreased(t =5.31,5.92,4.61,4.22,all P ＜ 0.05),SV,LVEF and 6 minutes walking distance increased significantly(t =7.22,4.78,8.78,4.56,4.11,4.05,all P ＜ 0.05) ; group HR,LVEDD were significantly lower than those in the control group(t =4.31,4.92,all P ＜0.05).SV,LVEF and 6-minute walking distance was significantly higher than that in the control group(t =4.31,4.92,4.11,all P ＜ 0.05).After taking drugs,the observation group NT-proBNP levels decreased significantly than before treatment (t =14.55,P ＜ 0.05),and significantly lower than that in the control group (t =7.12,P ＜ 0.05).No significant change in NT-proBNP levels before and after treatment in control group was found (t =1.03,P ＞ 0.05).The observation group in the total effective rate was 93.33 ％ (28/30),the control group was 63.33％ (19/30),the difference was statistically significant(x2 =5.26,P ＜ 0.05).5 cases of adverse reactions to the observation group,4 patients in the control group,no significant difference in the adverse reactions was found between the two groups (x2 =0.67,P ＞ 0.05).Conclusion Low dose,short-term use of milrinone can make the heart function of patients with refractory heart failure was obviously improved,significantly improve the efficacy with higher safety.

Objective To explore the expression of Pokemon protein and p53 protein in ovarian carcinoma and assure the relationship with clinicopathologic parameters.Methods 52 cases of ovarian cancer were enrolled as the observation group,60 cases of benign ovarian tissue as the control group.Expression of Pokemon protein and p53 protein were detected by immunohistochemistry.Results The positive rate of Pokemon protein were respectively 69.2％ (36/52) in the observation group and 11.7％ (7/60) in the control group,a significant differences between the two groups (x2 =12.74,P ＜0.01).The positive rate of p53 protein were respectively 78.8％ (41/52) in the observation group and 15.0％ (9/60) in the control group,a significant differences between the two groups(x2 =22.71,P ＜ 0.01).Pokemon protein expression were associated with ovarian cancer tumor invasion depth (x2 =9.16,P ＜ 0.05),clinical stage(x2 =5.86,P ＜ 0.05),lymph node metastasis (x2 =32.28,P ＜ 0.01) and recurrence (x2 =27.08,P ＜ 0.01) and no significant relationship with tumor differentiation (x2 =0.82,P ＞ 0.05).p53 protein expression were associated with ovarian cancer tumor invasion depth (x2 =8.24,P ＜ 0.05),clinical stage (x2 =9.24,P ＜ 0.05),lymph node metastasis (x2 =19.62,P ＜ 0.01) and recurrence (x2 =21.16,P ＜ 0.01) and no significant relationship with tumor differentiation(x2 =0.77,P ＞0.05).By correlation analysis,Pokemon protein expression had a significant positive correlation and p53 protein(r =0.297,P ＜ 0.05).Conclusion Pokemon protein and p53 protein had closely related with diagnosis,lesion degree and prognosis in ovarian cancer.

Objective To analyze the effect of artery perfusion intervention combined with radiation and chemotherapy in the treatment of locally advanced cervical cancer,and to provide clinical reference.Methods One hundred and eighteen cases with locally advanced cervical cancer patients were divided into observation group and control group by random digits table method,each group 59 cases,all patients in two groups received preoperative chemoradiation,observation group based on this,and added arterial perfusion interventional chemotherapy,compared the clinical curative effect of two groups.Results The effective rate in observation group was significantly higher than that in control group[93.22％(55/59) vs.77.97％(46/59)] (x2 =5.567,P=0.018).Seventeen cases in observation group with Ⅰ and Ⅱ degree of nausea,vomiting,2 cases with Ⅲ degree of nausea,vomiting,4 cases with Ⅰ degree of bone marrow suppression,the incidence of adverse reactions were 38.98％(23/59);18 cases in control group with Ⅰ and Ⅱ degree of nausea,vomiting,3 cases with bone marrow suppression,the incidence of adverse reactions were 35.59％ (21/59),there was no statistically significant difference between two groups (x2 =0.145,P =0.703).Conclusions Preoperative arterial perfusion intervention combined with chemoradiotherapy can effectively improve the curative effect for locally advanced cervical cancer,and has no increase in adverse reactions,and has important clinical value.

One hundred and fifty-two cases of fasciitis of lumber muscles were divided into two groups.The treatment group of 75 cares war treated with acupuncture and movement therapy bared on syndrome differentiation with meridians and collaterals using points of Shuigou(DU26)or Houxi(S13),Shousanli(LI10)or Tmchong(LR3);while 77 cages in the control group were treated with simple acupuncture and Ashi points and Weizhong(BL40).The total efficacy rate Wag 86.7%in the treatment group and 64.9%in the control group(P＜0.01).The effect of immediate pain relief in treatment group WaR better than that of the control group,with the VAS scores significantly decreased(P＜0.01).Acupuncture and movement therapy is effective for treatment of fasciitis of lumbar muscles.

Objective]Analysis of related theory that“the theory of spleen and stomach”treats“diet injury”, to explore principle-method-recipe-medicines’guiding significance for treatment of internal injuries caused by“diet injury”to explore the related theory’s guiding significance for treatment of internal injuries caused by“diet injury”(This is the related theory to“the theory of spleen and stomach”treating“diet injury”).[Method]Through the process of diagnosis and treatment of three cases analysis(They are“drinking wound”case, dizziness case and insomnia case), expound the argumentation thinking to“the theory of spleen and stomach”treating“diet injury”, principle of prescription medication, and matters needing attention, etc. Through the analysis of diagnosis and treatment process, expound the argumentation thinking to“the theory of spleen and stomach”treating“diet injury”, principle of prescription medication, and matters needing attention. Including the prescription of treatise on febrile disease———Wuling powder’s application in the“diet injury”,Gehuajiejiu soup, Zhishu pill, Hezhong pill’s application in the“drinking wound”.[Result] Understand related theory that the“the theory of spleen and stomach”treats “diet injury”, specific therapeutic principle for sweating, the urine and digestion. Including the prescription of treatise on febrile disease—Wuling powder’s application in the“diet injury”,Gehuajiejiu soup, Zhishu pill, Hezhongpill’s application in the“drinking wound”, Wuling powder, Gehuajiejiu soup are dehumidification by sweating and the urine, Zhishu pill, Hezhong pill ,etc are helping digestion.“The theory of spleen and stomach”treating“diet injury”,prescription and symptoms are consistent. Clinical efficacy of three consilias is satisfaction. Among them, drinking wound, shares proprietary Chinese medicine medicinal properties to achieve and anti-nausea, points up and down digestive purpose of alcohol. Gehuajiejiu soup points up and down digestive purpose of alcohol warning patients at the same time; The dizziness, Wuling powder and Banxiazhishu pill wet Xiaoshi with use and effect;Insomnia, emphasis will be placed on the medication method of small amount many times, and“Nursing mind modulation spleen and stomach”, cooperating with use, treatment effect is obvious. [Conclusion]Using the related theory of“the theory of spleen and stomach”is worth clinical exploration to treatment of clinical disease caused by the“diet injury”, because it is dialectical correctly, curative effect is better.

Objective To explore the early characteristics,risk factors and correct diagnosis procedure of acute pulmonary embolism.Methods The onset,axillary examination and susceptibility of 52 cases of acute pulmonary embolism between Jan 2004 and Dec 2005 were analyzed.Results The early manifestation of acute pulmonary embolism was difficulty in breathing,chest pain,hemota and fatigue.Only 11.5%patients presented all the obove symptomes at the same time.D-dimer＞500μg/L was 100%.Blood gas analysis showed hypoximia in 94.2%,typical SⅠQⅢTⅢin 13.6% on electrocardiograph,pulmonary artery hypertension in 82.5%on ultrasonography,dilated right in 38.5%of all the subjects.The diagnosis rate was 82.7%at the first diagnosis.Lower limb deep vein embolism,chronic cardiac,kidney disease,malignant tumor and periooperation patients were the risk factors.Conclusion Acute pulmonary embolism is specific-free of clincial manifestation,patients with history of vertigo but excluding difficulty in breathing,chest pain,hemato should go proper examination.

This article applies documentary,expert interview, questionnaire and other methods to study the physical education teaching of foreign students in Chongqing, and explores the construction of P.E features teaching content teaching to foreign students in medical colleges.

The encephalopathy of prematurity is a complex amalgam of primary destructive disease and secondary developmental disturbances.The interrelations between destructive and developmental mechanisms at cellular and molecular level in the genesis of the encephalopathy are emphasized and reviewed.We focus on its latest definition,microglia activation induced oligodendrocyte injury,neuronal/axonal impairments,and recent insights of thalamus,subplate neurons and cerebral cortex injury.