Objective To introduce and evaluate the technique of laparoscopic live donor nephrectomy in renal transplantation.Methods Three donors underwent laparoscopic live donor nephrectomy, and 3 recipients received renal transplantation. The procedures were described in detail. Results The surgery was successfully performed in all 3 cases. Ischemia of the harvested kidneys was less than 8 min. All 3 kidneys were successfully transplanted and functioned well in 3 recipients up to now.Conclusion Laparoscopic live donor nephrectomy is a minimally invasive surgery. Advantages include less postoperative pain, shorter hospital stay and convalescence. The recipients also have a satisfactory renal function.