Objective To investigate the significance of the mutation of c-kit gene in the development,(clinical) pathology and prognosis of gastrointestinal stromal tumors(GISTs).Methods The technique of(PCR-SSCP) was used to detect ckit gene mutation in 106 cases of GIST,and 57 of the cases were(followed-up).Results The c-kit gene mutation rate in the whole group was 45.3%(48/106),and(3.4)%(2/41) in benign GIST,and 70.8%(46/65) in malignant GIST.The 1-,3 and 5-year survival rates and median survival time of the mutation positive group were all markedlly lower than those of the(mutation) negative group.A comparison of the parameters that can differentiate the benign GISTs from malignant ones such as size,histological necrosis,aggressive grade,nucleus mitotic count and nuclear discrepancy,showed significant difference between the of postive and negative gene mutation(P

Objective To assess the association between the depth of trophoblastic penetration into the tubal wall with serum concentrations of vascular endothelial growth factor (VEGF) and β-human chorionic gonadotropin (β-HCG). Meth-ods Eighty patients with a diagnosis of tubal pregnancy in the ampullary region underwent radical surgical treatment (sal-pingectomy), were included in this study. The serum levels of VEGF andβ-HCG were detected on the day of surgery. The se-rum level of VEGF was measured by ELISA. The serum level ofβ-HCG was quantified with a two-site immunofluorimetric assay based on the direct sandwichtechnique. Histological material was stained with Masson's trichrome to identify muscular fibers. Immunohistochemical staining was used for human placental lactogen (hPL) to identify intermediate trophoblast and determine the depth of trophoblastic invasion into the tubal wall. The ampullary pregnancies were classified histologically ac-cording to the depth of trophoblastic infiltration into the tubal wall. Results The mean serum values of VEGF andβ-HCG were significantly lower in patients with stage I tubal infiltration than those of stageⅡ, and which were significantly lower in patients with stageⅡthan those in stageⅢ(P<0.05). The threshold serum value of VEGF was 308.6 ng/L, the sensitivity was 100.0%and the specificity was 92.6%for stageⅠand stageⅡ. The threshold serum value of VEGF was 431.9 ng/L, the sensitivity was 79.3%and specificity was 79.2%for stageⅡandⅢ. The threshold serum value ofβ-hCG was 2 509.6 IU/L, the sensitivity was 91.7%and specificity was 81.5%for stageⅠand stageⅡ, and levels of 13 142.6 IU/L, 72.4%and 95.8%for stageⅡand stageⅢ. Conclusion The depth of trophoblastic penetration into the tubal wall is associated with maternal serum concentrations of VEGF andβ-HCG, which can be used as the evaluation index for histological staging of trophoblas-tic tissue infiltration.

Objective: To share the anti-infection experience for the patients with complicated intra-abdominal infection after operation.Methods: The whole course of drug administration participated by clinical pharmacist in one patient with complicated intra-abdominal infection after pancreatoduodenectomy were retrospectively analyzed and discussed.Results: Enterococcus was found in the blood culture and ascites.According to the results of drug sensitive tests, clinical pharmacist provided medication suggestions and performed medication mornitoring.According to the blood concentration, clinical pharmacist adjusted the dose of vancomycin, and changed the therapy regimen according to the changes of conditions.As a result, adverse reactions were controlled timely, and satisfactory treatment effects were obtained.Conclusion: The aetiology of complicated intra-abdominal infection is complex with intractable treatment, and the participation of clinical pharmacists in the treatment can improve the efficiency and safety.

Theinnate immunity system of human body has more and more attention for its antibacterial, antiviral, maintainingimmunehemostasis and promoting tissue damage and repair and other physiological functions.As members of NOD-like receptors(NLRs), NOD1 and NOD2 receptors are identified as intracellular pattern recognition receptors(PRRs), can be identified with molecular damage endogenous(damage-associated molecular patterns, DAMPs)and exogenous injury-molecular pathogen associated molecular(pathogen-associated molecular patterns, PAMPs), and initiation of innate and specific immune response, maintain the steady balance of body.Recently, a bunch of evidence have demonstrated that the importance of NOD1 receptor and NOD2 receptor is not limited in field of anti-infection, and the insulin resistance, kidney and liver damage recovery, cardiovascular disease and tumorigenesis are also closely related with these two receptors.So the aim of this article is to interpret the NOD1 and NOD2 general structure and function, and summarize the link between these two PRRs and tumorigenesis and finally make a clue for cancer immunotherapy.

Objective To compare the incidence of secondary surgical procedures after cervical disc arthroplasty vs anterior cervical discectomy with fusion in patients treated for symptomatic single level cervical spondylosis.Methods An online search of Pubmed, Medline, Ovid, Embase, Cochrane Library, CBM database, Wanfang data and VIP database were searched for prospective randomized controlled trial of cervical disc arthroplasty versus anterior cervical discectomy with fusion in incidence of secondary surgical procedures.Data were collected and extracted by two reviewers independently.Risk of bias was assessed using the criteria of Cochrane Reviews Handbook 5.1.0.Review Manager 5.2 software system was used to evaluate the data for Meta analysis.Results A total of 12 trials were included.Six of them were short-term follow up (two years).The 6 other trials were mid-and long-term follow up (mean 5.8 years).There are 4 trials with low risk of bias, 7 trials with moderate risk of bias, and 1 trial with high risk of bias.The results of meta-analysis showed there was no statistical difference in reoperation rate of adjacent level between the two groups at two years follow-up;however, the reoperation rate of non-fusion group was significantly lower in the non-fusion group compared with fusion group at mid-and long-term follow-up.The incidence of secondary surgical procedures at index level was higher in fusion group than in non-fusion group.No statistical difference was found between the two groups in using ‘ removal’ for secondary surgical procedure at two years follow-up;however, the rate of non-fusion group was significantly lower than fusion group at mid-and long-term follow-up.Conclusion Cervical disc arthroplasty was partly superior to anterior cervical discectomy with fusion in avoiding secondary surgical procedures of adjacent levels.However, non-fusion surgery doesn't have any advantages in short time (two years) follow up.Reducing the incidence of pseudarthrosis was an effective way to decrease the incidence of secondary surgery procedures in index level.

Serum Cardiac troponin Ⅰ (cTn Ⅰ) and lactic acid were measured in 128 neonatal patients with hypoxic-ischemic encephalopathy and 38 healthy neonates (control group). Serum cTn Ⅰ and lactic acid levels were higher in neonates with hypoxic-ischemic encephalopathy than those in healthy neonates (P < 0. 05 ); and the differences of these values were also statistically significant between the serious patients and the mild patients (P<0.05). Serum cTn Ⅰ and lactic acid are two sensitive markers for degree of myocardial injury and hypoxia in neonatal patients with hypoxic-ischemic encephalopathy.

ObjectiveTo revise and test the reliability and validity of the Chinese vision of the Basic Needs Satisfaction in General Scale (BNSG-S).MethodsTotally 6366 youth workers from 5 organizations were recruited with stratified sampling method.Participants were asked to complete the Chinese vision of BNSG-S.Item analysis was conducted to identify valid items and revise the scale.Then the construct validity of the revised scale was tested through exploratory and confirmatory factor analysis ( EFA & CFA).The criterion validity was tested with the Index of Well-being Scale.Finally the reliability of the scale was examined.ResultsThe revised scale consists of 15 items with 4 factors- satisfied state of autonomy need,blocked state of autonomy need,satisfied state of relatedness need,blocked state of relatedness need.The four factors explained 63.95％ of the total variance.Besides,the CFA results demonstrated a satisfactory construct validity of the scale ( RMSEA =0.056,NFI =0.97,NNFI =0.96,CFI =0.97).The correlation coefficients between the scale and the Index of Well-being Scale was 0.50 (P＜0.01 ) and each factor was significantly correlated to the Index of Well-being Scale.The Cronbach's α coefficients of the Chinese vision of BNSG-S was 0.76,and those of its 4 factors were 0.80,0.67,0.83and 0.80 respectively.ConclusionThe Chinese vision of BNSG-S with 4 factors has good reliability and validity,demonstrating it a valid tool to measure Chinese basic needs satisfaction.

Objective To evaluate the relationships of the expression of orexin A in hypothalamus with hyperleptinemia, hyperinsulinism, and hyperlipidemia in alimentary obesity rats.Methods The alimentary obesity rat model was induced by high-fat diet in Wistar rats. The levels of leptin, insulin, C-peptide, total cholesterol (TC) and triglyeride (TG) were detected by luminescent immunoassay and biochemistry enzymic method. The gene expression of orexin A in rat hypothalamus was detected by real-time PCR. The correlation between orexin A and leptin was analyzed. Results After 8 weeks of high-fat diet feeding, the body weight, Lee's index, blood glucose, TG and TC significantly increased in obesity rat group (P＜0. 01). The levels of serum leptin, insulin and C-peptide were significantly higher in obesity rats than in controls [( 1.74±0. 36)μg/Lvs. (1.27±0.11) μg/L, (35±5) MIU/L vs. (23±4) MIU/L, (0.21±0.04) μg/L vs.(0. 14±0. 03) μg/L respectively, P＜0.01]. However, the level of orexin A mRNA was lower in obesity rats than in controls (6.8±2.3 vs. 14.5±3.6, t=-8.06, P＜0.01). The correlation coefficient (r) of orexin A mRNA with serum leptin, insulin, and C-peptide in alimentary obesity rat were -0. 726 (P＜0.01), -0. 506 (P＜0.01) and -0. 664 (P＜0.01), respectively. Conclusions High-fat diet can induce leptin-resistance and alimentary obesity in Wistar rat. The down-regulation of orexin A in rat hypothalamus would be correlated closely to the leptin and insulin resistance in alimentary obesity.

Objective To investigate the lifestyles of internet addiction disorders and explore the relationship between lifestyles and internet addiction among Chinese adolescents.Methods In this cross-sectional stratified cluster sampling study,Demographic Information Questionnaire,Chinese Adolescent Lifestyles Scale (CALS)and Young's Internet Addiction Test were applied.Results (1) Adolescents with internet addiction were 275,accounting for 11.20％.Boys(15.45％) addicted the internet more than girls(7.48％).The prevalence rate of IAD was higher in students from urban(13.55％) than that in students from rural area(9.67％).The ratio of IAD was the highest in junior school students (14.01％),the second in university students (13.84％),the third in senior high school students(9.66％),and the lowest in primary school students(7.67％) (P＜0.01).(2) The internet addicts scored significantly lower on sleep,study habits,physical exercise,interpersonal relationship and coping style,compared to the normal group(P＜0.01).(3) Sleep status,study habits,physical exercise,interpersonal relationship and coping style were negatively correlated with internet addiction (r=-0.193,r=-0.319,r=-0.175,r=-0.225,r=-0.105,P＜0.01).(4)Multiple logistic regression analysis indicated learning habits and interpersonal relationship had negative predictive effects on internet addiction; whereas,the coping style showed a weak positive prediction effect on Internet addiction.Conclusion Internet addict teenagers have poor lifestyles which should be suggested to start the good behavior habit and insist on a healthy lifestyle to prevent the internet addiction disorder.

Objective To develop a father involvement scale and test the reliability and validity.Methods The scale was developed based on previous studies and depth interviews with more than 20 fathers.Totally 1565 families from 3 provinces were recruited.Item analysis was conducted to identify valid items.Then the construct validity of the scale was tested by exploratory and confirmatory factor analysis (EFA & CFA).Finally the reliability and criterion validity of the scale was examined.Results The scale was consisted of 8 factors wihch were involvement attitude,activities and playing,accompany and taking care of children,attentiveness,discipline and school encouragement,economic support,protection,support mother.They explained 59.94％ of the total variance.Besides,the CFA results demonstrated a satisfactory construct validity of the scale (RMSEA =0.05,NFI =0.95,NNFI =0.96,CFI =0.97).The Cronbach(')s α coefficients of the scale was 0.897,and those of its 8 were 0.828,0.788,0.764,0.781,0.671,0.754,0.528 and 0.693 respectively.Conclusion The Father Involvement Scale with 8 factors has a good reliability and validity,demonstrating it a valid tool to measure father involvement in China.