Natriuretic factors,include cardionatrin(CN)and digoxin-like immunoreac-tive substances(DLIS),were important humoral factors relating to ascites formation inliver cirrhosis.An observation was made on the levels of plasma CN,DLIS,angiotensinⅡ, aldosterone and serum and urine sodium contents berore and after ascites extinction in40 cirrhotic patients.The results showed that the plasma levels of CN and DLIS had dis-tinct changes before and after ascites extinction and their changes were parallel with theactivity of renin-angiotensin-aldosterone system.These facts suggested that CN and DLIShad certain relation with ascites formation in liver cirrhosis.

Objective To observe the effect of acupuncture combined with massage on vertebral artery cervical spondylosis.Methods 100 patients with vertebral artery cervical spondylosis were randomly divided into the treatment group and control group with 50 cases in each group.The cases of the treatment group were treated with acupuncture neck to Jiaji,Baihui,Wind Point acupoints and massage therapy.The cases of the control group only took sibelium orally.Results The cure rate and total effective rate of the treatment group were 70.0% and 98.0% respectively;those of the control group were 51.1% and 86.0%.There was a significant difference in cure rate and total effective rate between two groups(P<0.05～0.01).Conclusion Acupuncture combined with massage treatment is effect on vertebral artery cervical spondylosis.

Objective To analyze the distributional characteristics of NPC1L1 1 735 C>G polymorphism and the relation-ship between serum lipid levels and obesity in Guangxi Han population.Methods Genotyping of the NPC1L1 1 735 C>G poly-morphism of 409 cases of Han was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis in all subjects.The relationship of different genotypes in blood lipid levels and obesity were evaluated.Re-sults The frequency of CC,CG and GG genotypes was 39.40%,46.00% and 14.60% in Han.The frequency of C and G alleles was 62.35% and 37.65% in Han.The levels of BMI,LDL-C,ApoB was different among the three genotypes (P <0.05).The G al-lele carriers had higher serum LDL-C and ApoB levels than the G allele noncarriers (P <0.05 ).Serum lipid parameters and BMI were also correlated with some environmental factors.Conclusion The 1 735 C>G polymorphism in NPC1L1 gene may be correla-ted with serum lipid profiles and obesity,the G allele might increase the risk of hyperlipemia and obesity.

Objective To compare of flexible ureteroscope holmium laser lithotripsy and percutaneous nephrolithotomy in treating renal stones of ≤2cm.Methods In this retrospective study,we compared 120 patients of renal pelvic stones of ≤2cm size treated with FURS(n=62)or PCNL(n=58).Factors such as operation time,hospitalization time,stone-free rate(SFR),drop in haemoglobin level,and complications were analyzed.Results The stone burden of the FURS group and PCNL group was(1.4±0.2)cm and(1.6±0.3)cm respectively.In FURS group,The operation time was(28±12)min with postoperatlve fever in 3 cases;he hospitalization time was(3.7±1.8)days.The decrease of hemoglobin was(0.18±0.06)g/L.No major complication was recorded.The SFR was 85.5%(53/62)one week postoperatively and 95.2%(59/62)one month postoperatively.In PCNL group,the operation time was (42±15)min with postoperative fever in 2 cases and bleeding in 2 case.The hospitalization time was 6.8±1.5 days.The decrease of hemoglobin was(14.30±3.50)g/L.The SFR was 91.4%(53/58)one week postoperatively and 96.6%(56/58)one month postoperatively.Conclusion For renal stones≤2cm,FURS has the advantages of shortening operation time,reducing blood transfusion requirements,decreasing postoperative complications with a farely SFR compared with PCNL.FURS is a good alternative.

Objective To characterize the abrasive wear of polymer tapes in ATM (Auto Teller Machine)servicing in sand dust environment. Methods The abrasive wear experiment of polymer tapes, PEN (polyethylene-2,6-naphthalenedicarboxylate) and PI (polyimide), by sand dust particles is performed in ATM tribosystem. The fractal characteristics of the worn surface of polymer tapes and the corresponding wear mechanisms are examined. Results As main results, the worn surfaces of polymer tapes show bi-fractal characteristics, and PI tape shows higher fractal dimensions and finer wear profiles than PEN tape. Wear mechanism examination shows macro and micro ploughing and cutting grooves and micro fracture characters, which gives reasonable explanation of bi-fractal character of worn polymer tapes. Conclusion Worn polymer tape shows fractal character and bi-fractal can be used in accurately characterizing the abrasive wear of polymer tapes of ATM in sand dust environment.

Objective:To prepare specific IgY production using Actinobacillus actinomycetemcomitans(A.a) immunizing hen, and then to investigate anti-Actinobacillus actinomycetemcomitans IgY inhibiting growth of A.a and Capnocytophaga gingivalis(C.g). Methods:Using immunization method, water-dilution method, two-step ammonium sulfate precipitation, inhibiting bacteria growth test in liquid anaerobic culture, and ELISA, IgY were induced, extracted, purified, and inhibiting growth of A.a and C.g by the IgY was roundly evaluated. Results:The IgY purity reached to 85.6%~90.3% through 550 g/L and 330 g/L ammonium sulfate precipitation, and efficacy value was 1∶32 000. The IgY efficacy value of anti- A.a was 1∶8 000 against C.g in cross-reactivity.When IgY concentrations of anti-A.a were in the 5.0,1.0,0.1 g/L and concentration of A.a was in the 5?108 CFU/L, the suppression rate of A.a growth were 31.60%(P=0.004),10.24%(P=0.024),-3.30% respectively during 24 h culture and were 64.20%(P=0.004),53.21%(P=0.002),11.20% respectively in 72 h culture. When the concentration of A.a was in the 1?108 CFU/L, the suppression rate of A.a growth were 35.71%(P=0.004),30.95% (P=0.012),11.11% respectively during 24 h culture, and were 65.11%(P=0.005),54.04%(P=0.002),16.17% respectively during 72 h culture. When 5.0 g/L IgY of anti-A.a was cultured with 1?108 CFU/L C.g for 24 h, the suppression rate of C.g growth was 41.61%(P=0.005), and for 72 h it was 86.99%(P=0.014). Conclusion:The hen is able to be induced to produce high efficacy value IgY of anti-A.a by A.a. The specific IgY of anti-A.a is capable of inhibiting A.a and C.g growth. There are common antigens and cross immunizing reactivity between A.a and C.g.

Objective:to do neuropsychological tests and apply functional questionnaire in screening of Alzheimer disease in rural area of north China. Method: subjects positive in screening with MMSE were applied a battery of neuropsychological tests including Fuld Object Memory test (FOM), a categorical test (Animal Naming Test), Digit Span Subtest from WAIS-R, and Block Design Subtest from WISC-III. Examination of apraxia and Pfeffer Functional Questionnaire were also applied.Result:There were significant differences of FOM in different groups, such as Alzheimer disease (AD) group, vascular dementia (VD) group, depression group, and VD+AD group. FOM had better sensitivity and specificity, while the sensitivities of RVR and apraxia were low, and the specificity of Digital Span and Block Design were not high enough.Conclusion:FOM may play an important role in diagnosis of Alzheimer disease.

Objective To explore the feasibility and value of laparoscopic cholecystectomy (LC) for acute cholecystitis complicated with incarcerated gallstones. Methods From January 2005 to December 2007,71 patients with acute cholecystitis complicated with incarcerated gallstones received laparoscopic cholecystectomy by using four trocars in our hospital. The Arteria cystica and ductus cysticus were operated successively. For widened ductus cysticus,we ligated the duct first and then cut it off. Afterwards,the wound on the liver bed and its margin were electrocoagulated. After the operation,a drainage tube was placed under the liver. Results Of the patients,the LC were successfully completed in 67 cases (94.4%),4 cases were converted to open surgery (2 of them had a disease course of less then 3 days,and one over 3 days). No biliary injury or death occurred in this series. The patients were discharged from hospital in 2-6 days (mean,3 days). No complication was found during a 6-month follow-up. Conclusions With the improvement of operator's proficiency and skills,LC becomes feasible and safe for acute cholecystitis complicated with incarcerated gallstones.

[ ABSTRACT] AIM:To investigate the effect of microRNA-375 ( miR-375) on the viability, cell cycle and apop-tosis of HCT116 cells.METHODS: The expression of miR-375 in different colorectal cancer cell lines was detected by real-time PCR.The miR-375 mimics was transfected into HCT116 cells by LipofectamineTM 2000.The mRNA expression of miR-375 and AEG-1 was detected by real-time PCR.The HCT116 cell viability was detected by MTT assay.The changes of apoptosis and cell cycle distribution were analyzed by flow cytometry.RESULTS:Real-time PCR showed that miR-375 expression was the lowest in HCT116 among 4 colorectal cancer cell lines.The expression level of miR-375 significantly in-creased in miR-375 mimics group compared with that in the negative control group.The high expression level of miR-375 significantly inhibited the mRNA expression of AEG-1.After transfection with miR-375 mimics, the cell viability was in-hibited, the apoptotic rate was increased, the proportion of G1-stage cells was increased, and the proportion of S-stage cells was decreased.CONCLUSION:miR-375 inhibits the viability, mediates the cell cycle arrest and promotes the apoptosis of colon cancer HCT116 cells.miR-375 may act as a tumor suppressor in colorectal cancer by inhibiting AEG-1.