Objective To investigate the capability of bluetongue virus(BTV)dsRNA inducing IFN-β from human cells.Methods Artificial complex interfemn inducer polyinosinic-polycytidylic acid (PolyI:C).BTV and BTV dsRNA were added to A549(human lung cancer cell)and HEL(human lung normal cells)culture system in difierent concentrations.IFN-β in culture median was detected by ELISA.Results Though all of the 3 reagents could induce IFN-β,BTV dsRNA significanay induced the highest level of IFN-β.The production of IFN-β was induced by BTV dsRNA in dose dependence.BTV dsRNA induced IFN-β level from HEL Was higher than that from A549(P<0.05).Conclusion BTV dsRNA Can induce IFN-β from human cells effectively,which shows its potential of an endogenous IFN-β inducer.

Object To investigate the main risk factors of ICU sydrome,and establish Logistic regression model to evaluate the accuracy rating、sensitivity and specificity for predicting ICU sydrome.Methods Investigated all patients hospitalized in ICU from June to July of 2007.Data collected include age,gender,religion,family income,education degree,primary disease,character type,APACHE II score and et al.Case-control study Was used to retrospectively investigate the clinical data of ICU sydrome.Firstly,sillgle factor analysis was used in 21 clinical variables.Then,Multivariate Logestic regression analysis was used in selected variables.Results Twelve clinical variables,including age,gender,family income,health care cost wiping out degree,whether or not having family member'death,whether or not encountering other patients'death,length of stay in ICU,whether or not receiving ventilator theraphy,sleep quality,score of APACHE II,time with disease,character type.By Logestic regression analysis,it WSS revealed that there were 6 significant factors for ICU sydrome in diminishing sequence as whether or not receiving ventilator theraphy,length of stay in ICU,sleep quality,gender,time with disease and whether or not encountering other patientsMeath.Logestic regression equation was as following:P=1/[1+Exp∑(31.425+2.611X1+8.188X2-2.677X3+7.474X4+4.312X5+6.153X6)].The accuracy rating、sensitivity and specificity of the Logestic model was 82.37%.79.42% and 85.13% respectively.Conclusions Whether or not receiving ventilator theraphy,length of stay in ICU,sleep quality,gender,time with disease and whether or not encountering other patients'death were the main risk factors of ICU sydrome.The Logestic regression model based on these factors is reliable in predicting ICU sydrome.

Objective To investigate the relationship between serum 1 ,25‐hydroxy vitamin D3 concentration level and children with bronchial asthma .Methods 222 children with bronchial asthma treated in paediatrics of Zhongshan Boai hospital from 2012 Jan 2014 Dec were chosen as asthma group(A) ,while 223 cases of healthy children were selected as control group(C) .Electro‐chemical luminescence method was used to detect the serum level ,and its effect on asthma was disscussed .Results The concen‐tration of serum 1 ,25‐hydroxy vitamin D3 of asthma group (86 .30 ± 27 .70 nmol/L) was low than the control group ( 93 .39 ± 27 . 15nmol/)L .Serum levels of asthma group in different age groups (A1 ,A2 and A3) were 106 .87 ± 28 .28 nmol/L ,88 .98 ± 20 .65 nmol/L ,68 .38 ± 19 .23 nmol/L respectively ,While those of control groups were (110 .00 ± 22 .30) nmol/L ,(87 .36 ± 24 .42) nmol/L ,(21 .23 ± 16 .42) nmol/L respectively .Male and female serum level in asthma group were (88 .26 ± 27 .73)nmol/L and (81 .94 ± 26 .91)nmol/L respectively .While those of control group were(91 .91 ± 26 .57)nmol/L and (85 .69 ± 28 .04) nmol/L re‐spectively .Conclusion There is significant correlation between serum 1 ,25‐hydroxy vitamin D3 level and children with bronchial asthma .,yet no correlation between serum level and gender .1 ,25‐hydroxy vitamin D3 duficiency is one of the risk factors of chil‐dren asthma .

A wide range of pharmacological and clinical studies have been made on compound Ejiao Jiang in enriching blood, enhancing white blood cell, adjuvant chemotherapy, et al. By reference to relevant documents, this study summarizes current researches on compound Ejiao Jiang in terms of pharmacological and clinical application, providing literature basis for further studies on compound Ejiao Jiang.
Animals ; Blood Circulation ; drug effects ; Drug Therapy ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Humans