1.Optimization of Determination Methods for Scopoletin from Caulis Erycibes
Cuiting LIU ; Dongcheng ZHENG ; Caiqun HE ; Jie PAN ; Ping YAN ; Ruoting ZHAN
Journal of Guangzhou University of Traditional Chinese Medicine 2015;32(6):1067-1070,1074
Objective To optimize the high performance liquid chromatography (HPLC) method for determining the content of scopoletin from Caulis Erycibes. Methods Methanol-25% HCl ( v/v, 4 : 1) solvent was used to extract scopoletin. HPLC method was performed on Waters XBridge Shield RP18 column (4.6 mm × 250 mm, 5μm) with the mobile phase consisting of acetonitrile ( A) and 0.16% ( v/v) acetic acid ( B) solution by gradient elution. The detection wavelength was 298 nm and the flow rate was set at 1.0 mL/min. Results The linear range of scopoletin from Caulis Erycibes was 2.83-118 μg/mL, and the recovery rate was 99.47% ( sR=1.07%). Conclusion The optimized method is simple, specific and accurate, and can provide reference for content determination of scopoletin in Caulis Erycibes.
2.Advances in pancreatic β-cell protective strategies
Jing WANG ; Hongxia LIU ; Dongcheng CAI ; Chaoyong HE
Journal of China Pharmaceutical University 2020;51(5):622-627
Pancreatic β cells, the only cells in the body that can synthesize and secrete insulin, can be damaged by a variety of pathological factors. Increasing attention has been paid to the studies of protecting pancreatic β cells in the treatment of diabetes. In this review, we highlight the studies that focus on enhancement of islet β cell function, reduction of islet β cell apoptosis, increase of islet β cell number and induction of islet β cell differentiation, providing novel targets and therapeutic strategies for the treatment of diabetes.
3.Effects of flattening filter on dosimetry in fractionated stereotactic radiotherapy for brain metastases
Zhijian ZHU ; Xiaoye ZHANG ; Yan ZHANG ; Tingting SHI ; Jun HONG ; Dongcheng HE ; Jihua HAN
Chinese Journal of Radiological Health 2022;31(5):615-619
Objective To investigate the dosimetric differences between volumetric modulated arc therapy (VMAT) with a flattening filter (FF) and flattening filter-free (FFF) VMAT in fractionated stereotactic radiotherapy for brain metastases. Methods Seventeen patients with brain metastases were divided into FF-VMAT group (VMAT plans with the FF mode) and FFF-VMAT group (VMAT plans with the FFF mode). The two groups were compared in terms of target volume dose parameters (D98%, D2% and Dmean), the conformal index (CI), the gradient index (GI), the gradient, normal brain tissue dose parameters (V5Gy, V10Gy, V12Gy and Dmean), monitor units, and beam-on time. Results Compared with the FF-VMAT group, the FFF-VMAT group had significantly lower GI (3.33 ± 0.37 vs 3.27 ± 0.35, P = 0.001), a significantly lower gradient [(0.85 ± 0.20) cm vs (0.84 ± 0.19) cm, P = 0.002], a significantly shorter beam-on time [(177.05 ± 62.68) s vs (142.71 ± 34.59) s, P = 0.001], and significantly higher D2% [(65.69 ± 2.15) Gy vs (66.99 ± 2.03) Gy, P = 0.001] and Dmean [(58.77 ± 1.60) Gy vs (59.95 ± 1.43) Gy, P <0.001]. There were no significant differences in the CI, the D98% of the target volume, the V5Gy, V10Gy, V12Gy and Dmean of the normal brain tissue, and monitor units between FFF-VMAT and FF-VMAT. Conclusion FFF-VMAT can better protect the normal tissue around the target volume, reduce the beam-on time, and improve treatment efficiency.
4.Infection status of enterovirus 71 and coxsackievirus A16 among children receiving health examination for child care setting entrance in Beijing and their related medical care seeking practice.
Xiaoli WANG ; Changying LIN ; Haiyan ZHANG ; Jianxin MA ; Chao LI ; Jie LI ; Lei JIA ; Yang YANG ; Yiwei DU ; Zhichao LIANG ; Quanyi WANG ; Xiong HE ; Email: HEXIONG@VIP.163.COM.
Chinese Journal of Epidemiology 2015;36(7):730-733
OBJECTIVETo understand the infection status of enterovirus 71 (EV71) and coxsackievirus A16 (Cox A16) among children receiving health examination for child care setting entrance in Beijing and their related medical care seeking practice and provide evidence for the estimation of disease burden caused by hand foot and mouth disease (HFMD).
METHODSSerological survey was conducted in the local children receiving health examination for child care setting entrance. Enzyme-linked immunosorbent assay (ELISA) was conducted to detect anti-EV71 and anti-Cox A16 IgG and IgM.
RESULTSA total of 813 children were surveyed (mean age: 3.5 ± 1.0 year old). The seropositive rate was 61.9% and 4.4% for anti-Cox A16 IgG and IgM. The seropositive rate was 9.3% and 1.1% for anti-EV71 IgG and IgM. No significant difference was observed in sex specific seropositive rate (P > 0.05). However, significant differences were found in seropositive rate among different age groups (P < 0.05). Among the children who were anti-Cox A16 positive, 7.8% had ever had rashes on their hands and feet, mouth or buttocks (HFMD-like rashes). Among the children who were anti-EV71 positive, 10.7% had ever had HFMD-like rashes. For the children who were anti-Cox A16 or anti-EV71 positive, only 7.1% were brought to see doctors by their parents. However, among the seropositive children with rashes, 80.5% were brought to see doctors.
CONCLUSIONIn the healthy children at the age to go to child care setting in Beijing, most had ever infected with Cox A16. The anti-EV71 positive rate was much lower than the anti-Cox A16 positive rate. It was necessary to strengthen the prevention and control of EV71 infection in child cares settings.
Antibodies, Anti-Idiotypic ; blood ; Beijing ; epidemiology ; Child Health Services ; utilization ; Child, Preschool ; Cost of Illness ; Enterovirus A, Human ; isolation & purification ; Enzyme-Linked Immunosorbent Assay ; Female ; Hand, Foot and Mouth Disease ; epidemiology ; virology ; Humans ; Male ; Parents ; psychology ; Patient Acceptance of Health Care ; statistics & numerical data