Objective To evaluate the clinical features,diagnostic methods and treatment of venous thromboembolic disease (VTE) during pregnancy.Methods From June 2006 to June 2011,a total of 20 pregnant women were diagnosed VTE at the Department of Obsterics and Gynaecology,Qilu Hospital of Shandong University.Clinical data of these patients were analyzed retrospectively.Results (1) Characteristics of patients:the symptoms of all the 20 patients commenced in pregnancy.Of these,6 (30％) happened in the first trimester,7 (35％) in the second trimester,and 7 (35％) in the third trimester.Twelve (60％) patients went to hospital in one week after they had symptoms,while 8(40％ ) went to hospital after one week.(2)Clinical manifestation:18 patients were diagnosed deep venous thrombosis (DVT),one was diagnosed pulmonary embolism (PE).One patient was diagnosed DVT and PE simultaneously.Among the 19 DVT patients,16 (16/19)were on the left side,3 (3/19)were on the other.They all came with sudden swelling and pain of the affected lower extremity.In 17(17/19) patients,the circumference differences between two legs were beyond (4.0 ±0.5) cm.In all the 20 patients,12 (60％) had elevated plasma level of D-dimmer.The diagnosis of DVT was made mainly by a Doppler ultrasound.Among the 19 DVT events,7 (7/19) were proximal DVT,2 (2/19) were distal,and 10( 10/ 19) were mixed type.(3)Anticoagulant therapy:patients with VTE during pregnancy were treated with low molecular weight heparin (LMWH) (enoxaparin,once 1 mg/kg subcutaneous,twice a day).After delivery,patients were treated with subcutaneous LMWH and warfarin simultaneously for at least 5 days,until the prothrombin time-international normalized ratio ( PT-INR ) was ＞ 2.0 for 24 hours.( 4 ) Thrombolytic therapy:for most patients with VTE,we are against the routine use of thrombolytic therapy,especially before delivery.For patients with acute massive PE,urokinase of 600 000 units intravenously daily was recommended for 3 days.For those patients with DVT whose standard anticoagulation therapy was ＜ 30 days,an inferior vena cava filter(IVCF) placement was recommended before delivery or abortion.If it was ≥30 days,IVCF was not recommended as a routine,and anticoagulant therapy was used 24 hours after delivery.If there was no recurrent DVT or PE,IVCF was retrieved routinely in 12 days.(5)Outcome:among patients treated with LMWH (95％,19/20).Three received IVCF placement,which was retrieved successfully in 12 days,with no interventional complication.All patients recovered well after 2 weeks,and the circumference differences between two legs were within (2.0 ± 0.3 ) cm.Of the 18 patients maintained to the third trimester,17 received anticoagulant therapy,and no abnormal findings were found during antenatal examination.Ten patients received cesarean section (50％,10/20),while 8 had vaginal delivery (40％,8/ 20).Neither neonatal asphyxia nor malformation was observed.The patients were followed-up for 1 - 24 months,no venous thrombus extension was found in 17 cases by Doppler ultrasound,thrombus disappeared in 2 cases of distal DVT after 4 weeks and 8 weeks respectively.By echocardiography,the pulmonary arterial pressure of the 2 patients with PE was found normal 3 months after hospital discharge.There was no maternal death during the study,no recurrent PE or bleeding occured.Conclusions LMWH is safe and effective for VTE during pregnancy.Routine use of thrombolytic therapy is not recommended.VTE in pregnancy is not the absolute indication of termination of pregnancy.The indication of an IVCF placement should be stricter,and a retrievable suprarenal IVCF is recommended under certain circumstances.

The mortality rate was measured against the time of the treatment of ozone and the variation of ozone concentration. Experiment results indicate that Ganoderma lucidum hyphae could be destroyed completely by ozone and the mortality rate obviously went up with the prolonging of treatment time. The changes of cell form and the fluorescence of hyphae (dyed by acridine orange or not) were observed through biological microscope, laser confocal scanning microscopic imaging system, transmission electron microscope, scanning electron microscope and atom force microscope, and the mechanism of Ganoderma lucidum hyphae (eucaryote) destroyed by ozone was studied.

Objective To know the cosmetics quality in Yunnan Province and to research the security countermeasure in order to protect the the health of people. Methods According to the state,standard test methods and criterion,the physical,chemical,biological and toxicological indicators of cosmetics were determined and evaluated. The results were treated by statistic methods. Results From 1996 to 2005,1 581 cosmetics samples were determined. The eligible rate of the cosmetics samples was 90.1%(1 425/1 581). The eligible rate was increasing from 1996 to 2005(?2=19.487,P

Objective: To observe the synergistic inhibitory effect of angiostatin gene combined with antisense hypoxia inducible factor-1? (aHIF-1?) gene on implanted human ovarian carcinoma in nude mice. Methods: BALB/C nude mice were subcutaneously transplanted with SKOV3 tumor cells and the tumors were allowed to grow till the diameter reached 0.4 cm, then the mice were randomly divided into 4 groups: PcDNA3 control group, PcDNA3-Angiostatin group, PcDNA3B-aHIF-1? group and PcDNA3-Angiostatin+PcDNA3B-aHIF-1? group; plamids PcDNA3, PcDNA3-Angiostatin, PcDNA3B-aHIF-1? and PcDNA3-Angiostatin+PcDNA3B-aHIF-1? were injected intra-tumorally in the above groups, respectively. The tumor samples were harvested on the 7 th day after gene transfer. Angiostatin, HIF-1?, vascular endothelial growth factor (VEGF) and microvessel density (MVD) of tumors were determined by immunohistochemical methods. Tumor cell apoptosis was determined with TUNEL staining. Results:The growth of tumors of PcDNA3-Angiostatin+PcDNA3B-aHIF-1? group was significantly inhibited, with local low expression of HIF-1? and VEGF (lower than those of the other 3 groups). MVD in combined transfection group(13.6?2.3) was lower than that of Angiostain group (24.5?2.7); the apoptosis index in combined transfection group (5.32?0.62)was higher than those of Angiostatin group(2.89?0.45), aHIF-1? group(2.98?0.51)and contrl group(1.56?0.41). Conclusion: Our results suggest a synergestic effect between Angiostain gene and aHIF-1? gene in inhibiting implanted human ovarian tumors in nude mice, which may contribute to drug resistance in antiangiogenic therapy of tumors.

OBJECTIVE: To test and estimate the forensic application of Goldeneye™ DNA ID 25A Kit. METHODS: The kit was validated by a series of tests for accuracy, sensitivity, consistency, peak height balance, stability, and mixed samples through measured blood samples and other samples in routine casework. RESULTS: The peak height balance of the different loci was ≥ 42%. The genotyping results of the positive control DNA was accurate. The complete STR genotyping result could be obtained from 0.125 ng positive control DNA. CONCLUSION Goldeneye™ DNA ID 25A Kit is suitable for criminal cases and DNA database in forensic practice.
Asian People/genetics* ; China ; Chromosomes, Human, Y ; DNA/genetics* ; DNA Fingerprinting/standards* ; Databases, Nucleic Acid ; Female ; Forensic Genetics/methods* ; Genotype ; Humans ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Reproducibility of Results ; Sensitivity and Specificity

Age Determination by Skeleton ; Body Height ; drug effects ; Child ; Child Development ; drug effects ; Drug Therapy, Combination ; methods ; Female ; Gonadotropin-Releasing Hormone ; administration & dosage ; analogs & derivatives ; therapeutic use ; Growth Hormone ; administration & dosage ; therapeutic use ; Humans ; Menarche ; Puberty, Precocious ; diagnosis ; drug therapy ; physiopathology ; Treatment Outcome

OBJECTIVE To investigate the antibiotic resistance status in clinical strains of Staphylococcus aureus (SAU) in Xiangfan and provide scientific evidence for reasonable use of antibiotics. METHODS Retrospective review was performed to analyzed the specimen source and the clinical distribution of 359 strains of SAU. BioMerieux Vitek 32 was used to identify the species of bacteria. Antibiotic susceptibility test was performed by K-B method and drug-resistance results were read according to CLSI2006. RESULTS Isolating rate of methicillin resistant S. aureus (MRSA) arrived at 54.9%. The results of susceptibility test showed that SAU had been resistant to the diverse antibiotics in different degree. The drug sensitivity rate of glycopeptide antibiotics and linezolid were all 100%. CONCLUSIONS The different grade hospitals should practically perform the management of antibiotics to postpone the resistance development and control outbreak and prevalence of nosocomial infections.

OBJECTIVETo study the effect of Shugan Jiangu Recipe (SJR) on bone mineral density (BMD) and serum bone metabolic biochemical markers in postmenopausal breast cancer patients with osteopenia.

METHODSTotally 38 patients of postmenopausal women with breast cancer, who received aromatase inhibitors (AIs), were assigned to the treatment group (21 cases) and the control group (17 cases) by using random digit table. All patients took Caltrate D Tablet (containing Ca 600 mg and Vit D3 125 IU), one tablet daily. Patients in the treatment group took SJR, 6 g each time, twice daily for 6 successive months. The bone mineral density (BMD) level was detected before treatment and at months 6 after treatment. Levels of bone alkaline phosphatase (BALP), bone gla protein (BGP), tartrate-resistant acid phosphatase (TRAP), and C-terminal telopeptide of type II collagen (CTX-II) were detected by enzyme linked immunosorbent assay (ELISA). The drug safety was also assessed.

RESULTSCompared with before treatment, BMD of L2-4 and femur neck obviously increased in the treatment group at month 6 after treatment (P < 0.01), serum BALP and TRAP decreased (P < 0.05). Compared with before treatment, BMD of L2-4 and femur neck obviously decreased in the control group at month 6 after treatment (P < 0.05), serum BALP and TRAP increased (P < 0.01). Compared with the control group, lumbar and femur neck BMD obviously increased, serum levels of BGP and BALP obviously decreased, and serum levels of CTX-II and TRAP obviously increased in the treatment group at month 6 after treatment (P < 0.01). No serious adverse event occurred during the treatment period. Bone fracture occurred in one case of the control group (5.8%).

CONCLUSIONSJR could attenuate bone loss of postmenopausal women with breast cancer who received AIs, increase BMD and improve abnormal bone metabolism.

Acid Phosphatase ; blood ; Aged ; Alkaline Phosphatase ; blood ; Aromatase Inhibitors ; adverse effects ; Bone Density ; drug effects ; Bone and Bones ; drug effects ; metabolism ; Breast Neoplasms ; drug therapy ; metabolism ; Collagen Type II ; blood ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Isoenzymes ; blood ; Middle Aged ; Osteocalcin ; blood ; Osteoporosis, Postmenopausal ; chemically induced ; prevention & control ; Peptide Fragments ; blood ; Tartrate-Resistant Acid Phosphatase

AIM: To investigate the effect of peribulbar anesthesia on intraocular pressure(IOP)and ocular amplitude pulse(OPA).METHODS: Thirty-two consecutive adult patients with monocular cataract enrolled in this study. IOP and OPA were measured with dynamic contour tonometer(DCT)before and 3, 10 minutes after administration of lidocaine anesthesia. Data were analyzed with software SPSS 11.5.RESULTS: The IOP remained stable in the injected eyes and the non-injected eyes after administration of lidocaine anesthesia. The OPA was significantly decreased after injection of anesthesia agent in the injected eyes. The OPA in the non-injected eyes increased significantly 3 minutes after injection of the anesthesia agent, returning to preinjection level 10 minutes after the injection.CONCLUSION: Peribulbar anesthesia leads to decrease of OPA and shows no effect on IOP in the injected eyes.

Five compounds (tenuifoliside C, tenuifoliside D, telephiose A, telephiose C and polygalaxanthone III) from polygala tenuifolia wild were incubated together with CYP probe substrate in human liver microsomes to investigate the inhibitory effect towards CYP450 enzyme. Phenacetin (CYP1A2), coumarin (CYP2A6), paclitaxel (CYP2C8), diclofenac (CYP2C9), S-mepheriytoin (CYP2C19), dextromethorphan (CYP2D6), chlorzoxazone (CYP2E1), midazolam (CYP3A) were selected as the isoforfn specific substrate. And the formation of paracetamol, 7-hydroxycoumarin, 6alpha-hydroxy paclitaxel, 4'-hydroxydiclofenac, dextrorphan, 6-hydroxychlorzoxazone, 1'-hydroxymidazolam, 4'-hydroxymephenytoin were detected respectively to measure the effect towards CYP450 by high-pressure liquid chromatography (HPLC). The result shows that five compounds from polygala tenuifolia willd significantly inhibit chlorzoxazone 6-hydroxylation catalyzed by CYP2E1, while showed no effect towards CYP1A2, CYP2A6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP3A. And IC50 value was 38.73, 54.14, 61.77, 62.22, 50.56 micromol x L(-1), respectively.
Cytochrome P-450 Enzyme System ; metabolism ; Esters ; pharmacology ; Glycosides ; pharmacology ; Humans ; Microsomes, Liver ; drug effects ; enzymology ; Oligosaccharides ; pharmacology ; Polygala ; chemistry ; Xanthones ; pharmacology