The effect of Alternariol Monomethyl Ether (AME), a suspected carcinogen in human being, on the lipid peroxidation in the epithelia of fetal esophagus and stomach was studied. The epithelia of esophagus and stomach of human fetus induced labor with water-bag were treated with AME. Lipid peroxides were measured by thiobarbituric acid test for malondialdehyde (MDA). There was a positive correlation between the values of MDA levels and AME dosaeges. It demonstrated that the lipid peroxdation could be initiated with AME. Lipid peroxidation levels were increased following treatment time elongating. The values of MDA levels in esophagus were higher than those in stomach, which were treated with the same dosaege of AME. It showed that AME had an organ selectivity. These results indicate that AME may be one of genetoxic etiological factors of esophageal carcinoma in Linxian county.

Objective To investigate the clinical efficacy of hemodialysis (HD) combined with hemoperfusion (HP) in the treatment of uremic pruritus.Methods The clinical data of 96 patients with uremic pruritus were analyzed retrospectively.All cases were treated with HD combined with HP.Results Pruritus score before and after treatment was (9.3 + 0.7) scores and (4.9 + 0.6) scores,respectively.And there was statistical significance (P ＜0.05).The erum creatinine,urea nitrogen,parathormone and β2 microglobulin before treatment were (712 ±148) μmol/L,(17.7 ±3.8) mmol/L,(47.1 ±12.4) ng/L,( 27.4 + 5.6 ) μ mol/L,respectively,and after treatment they were ( 324 ± 84 ) μ mol/L,( 7.4 ± 2.3 ) mmol/L,(15.6±5.1) ng/L,(11.1 ±2.2) μmol/L,there were statistical significance (P＜0.05).Conclusions HD combined with HP can effectively lighten the pruritus.It is worthy of clinical application.

A case of mandibular canine with apical periodontitis and double root canals was treated by CBCT assisted diagnosis and root ca-nal therapy.CBCT distinctly demonstrated the morphology of the root canal system in three dimensions and provided the information of canal varieties,prevented missed canals and conserved dentin.CBCT can guarantee the success of root canal therapy for the tooth with canal vareties.

Adult ; Crown Lengthening ; Dental Prosthesis ; Dental Prosthesis Design ; Esthetics, Dental ; Female ; Gingiva ; Humans ; Incisor ; Smiling

Adult ; Crown Lengthening ; Dental Restoration, Permanent ; Esthetics, Dental ; Female ; Humans ; Incisor ; Malocclusion ; therapy

Polo-like kinase 1(PLK1)is a serine/threonine kinase which is highly conserved, and its activity is elevated in many human tumor cell lines.Lots of reports have shown that PLK1 is critical for all stages of mitosis.The newest report finds that PLK1 is required for DNA synthesis, maintenance of DNA integrity, and prevention of cell death.PLK1 physically binds to the tumor suppressor p53 in mammalian cultured cells, and inhibits its transactivation activity as well as its function in activate checkpoint protein and its pro-apoptotic function.Also PLK1 is involved in the development of tumors, and Polo-like kinase1(PLK1)regulates IFN induction by MAVS, breakdown the innate immunity. Various of inhibitors of PLK1 show the feature of high performance and low toxicity, suggesting that PLK1 may be a feasible target for cancer therapy and immunity therapy.

Objective To investigate the related risk factors of prognosis in patients with systemic lupus erythematosus (SLE) complicated with lung infection.Methods The clinical data of 84 cases with SLE complicated with lung infection were analyzed retrospectively.Multivariate Logistic regressive analysis was made with death and survival as the independent variable.Results There were 23 out of 84 patients dead,and fatality rate was 27.38％.Multivariate Logistic regressive analysis showed that the urine volume,immune globulin treatment,cyclophosphamide pulse therapy,plasma albumin and SLE disease activity (SLEDAI) score were the risk factors that influenced the prognosis (OR =0.53,0.72,4.29,0.94,1.58,respectively,P＜0.0l or ＜0.05).Conclusions The prognosis of patients with SLE complicated with lung infection is the result of the joint action of many factors.The higher SLEDAI score and cyclophosphamide pulse therapy are the independent risk factors of the prognosis.The increase of plasma albumin level and urine production and immune globulin treatment are helpful to decrease the fatality rate.

Objective To establish a HPLC method for determining the content of Paeonol in Paeonia lactiflora Pall.. Methods Ultrasound and reflux methods were used to extract Paeonol in Paeonia lactiflora Pall., and then the content of Paeonol was determined by HPLC. It was performed on Agilent column ZORBAX SB-C18 (Stable Bond Analytical 4.6 mm?250 mm, 5 ?m), mobile phase was methanol-water (45∶55), flow rate was 1 mL/min, column temperature was 25 ℃, and detection wavelength was 274 nm. Results The regression equation was Y=1.01?105X-9.84?102, r=0.999 9 (n=5). The standard curve was linear within the range of 0.104～0.52 ?g for Paeonol. Conclusion The method is simple and accurate with good reproducibility. It is suitable for determining the content of Paeonol in Paeonia lactiflora Pall. and control the quality control of Paeonia lactiflora Pall. in process.

Objective To establish a method for the determination of bilirubin in Xihuang capsules.Methods HPLC method was applied and the chromatographic conditions were as follows: Hypersil BDS C18 column(150 mm ? 4.6 mm,5 ?m)with methanol-chloroform-1 % phosphoric acid(84︰9︰7)as the mobile phase.The flow rate was 1.0 mL ? min-1 with the detected wavelength at 450 nm and the column temperature at 25 ℃.Results The calibration curve for bilirubin was linear in the range of 0.01 ~ 0.07 ?g.The average recovery was 98.07 %(RSD = 1.05 %).Conclusion This method is simple,accurate,specific and could be used for the quality control of bilirubin in Xihuang capsules.

Objective To investigate the effect of total flavonoids from astragalus complanatus (FAC) on attenuating lung injury resulted from paraquat (PQ) poisoning by inhibiting excessive endoplasmic reticulum stress (ERS) and c-Jun N-terminal kinase (JNK) pathway in rat.Methods Forty-eight Sprague-Dawley (SD) rats were randomly divided into six groups (n=8 in each group),including control group,model group,dimethyl sulfoxide (DMSO) vehicle control group,and FAC in low,medium,and high dosage groups.The model was reproduced by giving PQ 80 mg/kg orally to induce lung injury.The rats in control group were treated with saline by gavage.The rats in DMSO group were given 10％ DMSO 20 mL/kg by gavage 2 hours before intraperitoneal injection of PQ,and those in FAC low,medium and high dosage groups received 40,80,160 mg·kg-1· d-1 of FAC solution intraperitoneally after the PQ administration.The rats were sacrificed 72 hours after giving PQ,and the left lung tissue was harvested 72 hours after the reproduction of experimental model.The ratio of wet/dry weight (W/D) and total lung water content (TLW) were determined.The pathohistological changes of the left lung was observed under light microscope,and scored with alveolar damage index of quantitative assessment (IQA).The mRNA expressions of JNK and glucose regulated protein 78 (GRP78) were determined by reverse transcription-polymerase chain reaction (RT-PCR),and the protein expression of JNK,phosphorylation-JNK (p-JNK),and GRP78 were determined by Western Blot.Results Compared with control group,the W/D ratio,TLW and IQA were increased significantly in model group and DMSO group,and the mRNA expressions of JNK and GRP78 and the protein expressions of JNK,p-JNK and GRP78 were markedly increased.Compared with the model group,the W/D ratio,TLW and IQA,and the expressions of JNK mRNA and p-JNK protein were significantly decreased in the FAC groups,especially in FAC high dosage group [W/D ratio:3.0 ± 0.3 vs.5.5 ± 0.5,TLW:2.2 ± 0.3 vs.4.7 ± 0.4,IQA:(15.4 ± 3.0)％ vs.(40.0 ± 5.7)％,JNK mRNA:0.21 ± 0.08 vs.0.82 ±0.27,p-JNK protein:0.31 ±0.09 vs.0.78 ±0.25,all P＜0.O1].The mRNA expression of GRP78 and the protein expressions of JNK and GRP78 were highly expressed in FAC low,medium and high dosage groups,and there was no significant difference compared with those in model group (GRP78 mRNA:0.54 ± 0.18 vs.0.74 ± 0.20,JNK protein:0.76 ± 0.27 vs.0.80 ± 0.28,GRP78 protein:0.51 ± 0.18 vs.0.69 ± 0.21,all P＞0.05).Conclusions PQ induces excessive ERS in the lung tissue resulting in lung injury.FAC has a protective effect on lung against PQ injury,and it may be related with inhibition JNK pathway in ERS.