Social forces participating in non-profit medical institutions will help to insist on the commonweal of public medical and health care and realize the goal of access to basic medical and health services for everyone .At present , social forces participating in non-profit medical institutions have made some prominent improvements under a series of policy support .However , due to the absolute dominance of public hospitals , social forces participating in non-profit medical institutions are still potting a lot of restrictions on access threshold of basic medical services , devel-opment space and policy implementation .In order to promote the healthy development of social forces participating in non-profit medical institutions , we should first recognize its significance and guarantee its development potential , tal-ents construction and development space through implementing preferential policies , improving supervision mecha-nisms, exploring multi-sited license, optimizing allocation of resources and so on .

OBJECTIVE:To establish a RP-HPLC method for determination of the content of hydrochlorothiazide tablets.METHODS:Samples were determined by RP-HPLC on a ZORBAX SB-C 18 column with a mixture of phosphate buffer solution(pH=4.0)-methanol(80∶20)as mobile phase and UV detection at226nm.RESULTS:The calibration curves of hydrochlorothiazide was linear from0.006to0.303mg/ml(r=1.0000,n=5),the average recovery and relative standard deviation were100.1%and0.43%(n=9)respectively.CONCLUSION:The method is rapid,simple,accurate and precise.It can be used for assay of hydrochlorothiazide.

Objective To evaluate the neuroradiological features of intraventricular central neurocytoma in ten patients.Methods Ten patients with central neurocytoma were imaged using contrast-enhanced and non-enhanced MRI scans,including 4 were also scanned with non-enhanced CT.The imaging data were studied with regard to location,size,margin,signal intensity,enhancement characteristics and presence of calcifications.Clinical data(i.e.presenting signs and symptoms,physical findings and medical history) were collected and histopathological and immunohistochemical studies were performed.Results There were 4 male,6 female in our group.All lesions were located in the lateral ventricles.Three tumors were confined to the left side,three to the right side and four involved both lateral ventricles.The growth of central neurocytoma was of close spatial relation to the septum pellucidum.In MRI,most of the cases showed a heterogeneous hypointensity on T1WI and hyperintensity on T2WI or FLAIR with a well-defined margin.The presence of cystic components,necroses and calcifications had been the cause of these internal heterogeneities.After intravenous administration of gadolinium(Gd-DTPA) all tumors showed a heterogeneous enhancement.CT provided additional information by distinguishing intratumoral calcifications in all four evaluated cases.Conclusion Central neurocytoma should be considered when young patients with lesions in the lateral ventricle,which contain calcifications and show some enhancement.This is especially applicable for tumors involving both lateral ventricles with symmetrical growth around the center of septum pellucidum or for unilateral ventricular tumors with a wide base attachment to the septum pellucidum.

Objective To explore the correlation between serum homocysteine (Hcy) level and diabetes mellitus combined with cerebral infarction (DMCI) in patients. Methods A total of 144 patients with type 2 diabetes mellitus (T2DM) were selected and divided into two groups, patients without cerebral infaction (group A, n=64) and patients with cerebral infaction (group B, n=80). Thirty healthy people were used as control group (group C). The serum Hcy level was detected by enzymatic cycling assay in three groups. The serum levels of cholesterol (TC) and triglyceride cholesterol (TG) were detected by enzymatic determination. The serum levels of high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) were detected by homogeneous phase method. The serum level of creatinine (Cr) was detected by creatine oxidase method. The level of uric acid (UA) was detected by urinary enzyme peroxidase coupling method. Glycosylated hemoglobin (HbA1c) was detected by high performance liquid chromatography. The carotid artery intima media thickness (IMT) was examined by color Doppler ultrasound, and patients were divided into mild and no stenosis group, moderate stenosis group and severe stenosis group according to the
results. The levels of Hcy were compared between all groups. The correlation of Hcy level and other indicators was analyzed. Results The levels of Hcy and HbA1c were group C0.05). There were no significant differences in UA and Cr levels between the three groups (P>0.05). The level of Hcy was positively correlated with age (rs=0.411), HbA1c (rs=0.219) and Cr (rs=0.242), and negatively correlated with gender (rs=-0.202) and HDL-C (rs=-0.278, P<0.05). There were no significant correlation between Hcy level and other variables (P>0.05). With the increased degree of carotid artery stenosis, the Hcy level and the proportion of HHcy showed a rising trend in patients (P<0.01). The level of Hcy was positively correlated with IMT(rs=0.781, P<0.001). Conclusion Hcy is a risk factor for the onset of DMCI. The high level of Hcy is closely related to the occurrence and progression of atherosclerosis. Hcy has great value for early screening and prevention of DMCI.

Aged ; Humans ; Kallikreins ; analysis ; Male ; Middle Aged ; Prognosis ; Prostate-Specific Antigen ; analysis ; Prostatic Neoplasms ; diagnosis

Objective To investigate the effect of ursodesoxycholic acid (UDCA) on JAK/STAT3/ COX-2 signal pathway in human hepatocellular carcinoma cells and the underlying mechanism of its antitumor effect.Methods In order to obtain the optimal concentration and time of UDCA,MTT assay was used to detect anti-proliferative activities of UDCA on SMMC-7721 cells induced by IL-6 in vitro.Cells were divided into control group,IL-6 group,AG490 (JAK2 inhibitor) group and UDCA group.The protein expressions of STAT3,p-STAT3 and COX-2 were measured by Western blot.Results MTT assay showed UDCA had obvious anti-proliferative activities on SMMC-7721 cells.The optimized concentrations were 50,100,200 μg/ml,the optimized culture time was 48 h.Western blot results showed that the expression of p-STAT3 and COX-2 were remarkably increased in IL-6 group than that in control group.There was no difference of STAT3 expression between the two groups.Compared with IL-6 group,the protein level of COX-2 was decreased in AG490 and UDCA groups,so was the expression of p-STAT3.But there was no change on STAT3 expression in AG490 and UDCA groups.Conclusion UDCA may decrease the proliferation of SMMC-7721 cells via inhibiting phosphorylation of STAT3 and down-regulating COX-2 exprcssion.

In vitro fertilization and embryo transfer(IVF-ET) technology is one of the important means to treat infertility in recent years,although it had made remarkable progress in the field of reproductive medicine,but IVF-ET pregnancy success rate is not very ideal,the reasons that influent the success of IVF-ET include both parents donor of various unfavorable factors and stimulate ovulation itself produces all sorts of malpractices,its influence factors are complicated.In recent years,how to reduce the adverse factors affecting IVF-ET,to improve the success rate of pregnancy of IVF-ET has become the hot topics in the study of domestic clinical intervention in IVF-ET,and the domestic research shown that the quality of the follicle and endometrial environment is the key to determine the success of IVF-ET.

In recent years,the influence of endemic arsenic poisoning on immune system has caught people's attention.Changes in histone modification patterns may be one of the mechanisms of arsenic regulated gene expression,arsenic plays an important role in differentiation,proliferation and function of immune cells.Arsenic has a certain effect on the differentiation and function of T cells,and it is rarely studied whether the process is regulated by histone methylation.In this paper,the study of histone methylation in arsenic induced T cellular immune damage is reviewed.

Objective To study the features of Kawasaki disease (KD)with liver damage and investigate the possible related factors,and to improve the understanding of liver damage in KD patients.Methods The clinical data of 75 KD patients admitted from 2008 to 2013 were collected to retrospectively analyze the clinical features of liver damage,as well as routine blood test,C-reactive protein (CRP),erythro-cyte sedimentation rate (ESR),liver function,and abdominal ultrasound.Categorical data were analyzed by chi-square test;continuous data were expressed as mean ±standard deviation;comparison between groups was made by t test and multivariate stepwise regression analy-sis.Results The incidence of abnormal alanine aminotransferase (ALT)level in 75 cases was 32%,and 79.2% of these patients had a serum ALT level of less than 200 IU/L,including 3 patients with hepatomegaly,1 patient with jaundice,and 1 patient with splenomegaly, which accounted for 12.5%,4.2%,and 4.2%,respectively,of patients with liver damage.CRP and ESR were not found to be related to liver damage (t=1.560,P=0.123;t=1.291,P=0.201).There was significant difference in platelet count between patients with liver damage and controls (t=-3.228,P=0.003).No significant difference in white blood cell (WBC)count increase was found between the liver damage group and control group,but simultaneous increase in WBC count and platelet count was significantly associated with an in-creased risk of liver damage (P<0.05).Conclusion CRP and ESR are not found to be significantly related to the development of liver damage,but there is a close relationship between liver damage and abnormal platelet count.WBC count shows a certain relationship with liv-er damage.Clinicians should pay attention to these factors and take timely measures for intervention.