Objective:To study the MRI features and its value in diagnosis and differential diagnosis of the vertebral multiple myeloma(MM).Methods: The MRI features of 16 patients with vertebral MM proved by histology were retrospectively analyzed.Results: The vertebral MM was classified into the following four types :Normal type accounted for 6.25%,in which the lesions were mild hyper-intensity on T1WI and iso-intensity on T2WI.Diffuse type accounted for 56.25%,in which the lesions were diffuse hypo-intensity with clear border on T1WI and hyper-intensity with indistinct border or iso-intensity on T2WI.Localized type accounted for 18.75%,in which the lesions were multiple nodular or patchy hypo-intensity on T1WI and mild hyper-intensity or iso-intensity on T2WI."Salt with pepper" type accounted for 18.75%,in which the lesions were diffuse spot-like mixed hyper-,iso-and hypo-intensity.The all lesions were hyper-intensity on STIR images.The involved vertebrae were continuous,which accounted for 68.75%.The vertebral bodies were metamorphosed in 5 cases(31.25%).The appendixes were involved in 4 cases(25%).The paravertebral soft tissue mass was found in only 1 case(6.25%).Conclusion: The vertebral MM has some features on MRI.MRI is valuable in diagnosis and differential diagnosis of the vertebral MM.

Objective:To investigate the CT manifestations of pulmonary inflammatory mass and peripheral lung carcinoma,and to improve the differentiation of the diseases.Methods:The CT manifestations of 26 cases of pulmonary inflammatory masses and 38 cases of peripheral lung carcinomas were retrospectively analyzed.All cases were proved by pathological or clinical appearances.The masses were divided into typical and atypical masses according to morphology.Studying the CT signs of lesions and combining them with the results of morphological classification improve diagnosis of the morphological classification.Results:According to the morphological classification of masses,the diagnostic sensitivity,specificity and accuracy of inflammatory mass were 65.4%,73.7% and 70.3% respectively.The improved sensitivity,specificity and accuracy were 80.8%,86.8% and 82.8% respectively.Conclusion:Combining the results of morphological classification of masses with the CT signs of lesions have important value in the differentiation of pulmonary inflammatory mass and peripheral lung carcinoma.

Objective To investigate the laboratory ifndings, clinical manifestations and treatment in hemolytic disease caused by red cell immune antibodies in neonates. Methods The laboratory and clinical data from 4 cases of hemolytic disease of neonates caused by red cell immune antibodies were retrospectively analyzed. Results IgG antibody were detected in all mothers of 4 cases during pregnancy and they were anti-E, anti-D, anti-Jkb and the autoantibody with the titer being 16, 2048, 1 and 16 respectively. The four neonates were all full-term. The jaundice appeared 6 h to 3 d after birth with varying degrees of skin stained yellow, with or without anemia. Serology and elution test found the existence of antibody same as the one on their maternal red cells and the titer was 4, 512, 0, and 2, respectively. All neonates were treated by phototherapy. Two servere cases were also treated by whole blood exchange and red blood cells transfusion. The prognosis were good in all neonates. Conclusions Prenatal immune hematological tests facilitated early detection of irregular erythrocyte antibodies and thus assessment of the risk of hemolytic dis-ease of the fetus and neonates.

AIM: The aim of the present study was to clar ify the mechanism of intracellular signal transduction in Ang II-induced prolife ration of vascular smooth muscular cells (VSMC) by observing the effect of c-Src on Ang II-mediated mitogen-activated protein kinase (MAPK) activation and c-Fos protein expression in cultured VSMC of rats. METHODS: Cultured aortic VSMCs from SD rats were transfected wit h anti-sense c-Src oligodeoxynucleotides (ODNs) wrapt with lipofectin to inhibit c-Src activity and protein production. Untransfected VSMCs were used as control . We observed the role of Ang II stimulation in MAPK activation and c-Fos protei n expression. c-Src kinase activity was measured by protein immunoprecipitation and kinase autophosphorylation. The phosphorylation rate of the substrate myeli n basic protein (MBP) was employed to assess MAPK activity. Western immunoblot w as used to detect protein expression of c-Src and c-Fos. RESULTS: c-Src protein expression in VSMC transfected with diffe rent concentrations of anti-sense ODNs significantly decreased in a negative dos e-effect manner. c-Src kinase activity was also markedly inhibited . Following t he stimulation of Ang II on transfected VSMCs with anti-sense ODNs, the increase rate of c-Src activity was 8.7% of that in control, the activity of MAPK was 1 .6% compared with control and c-Fos protein expression was as 30.0% as that of control. CONCLUSION: Ang II induces c-Src activation. MAPK activation and c-Fos protein expression by Ang II is dependent on c-Src activation. These find ings indicate that c-Src is an important signal factor in Ang II -induced VSMC proliferati on.

Objective:To explore the therapeutic effect and safety of direct and selective percutaneous coronary inter-vention (PCI)in patients with ST elevation myocardial infarction (STEMI).Methods:A total of 138 STEMI pa-tients were randomly divided into direct PCI group (n=69,received PCI immediately after diagnosis)and selective PCI group (n=69,received acute thrombolysis therapy and then PCI in a selected time).Left ventricular ejection fraction (LVEF),peak creatine phosphokinase (CPK),CPK peak time,peak creatine kinase isoenzyme (CK-MB), CK-MB peak time,cardiac rupture rate during hospitalization,re-infarction rate and mortality rate within 30d and one year were compared between two groups.Results:Compared with selective PCI group after treatment,there were significant rise in LVEF [(49.3±6.8)% vs.(58.2±7.7)%],peak CPK [(74.9±49.3)IU/L vs.(113.0± 59.3)IU/L ]and peak CK-MB [(1983.1±1251.2)IU/L vs.(2588.6±1592.3)IU/L],and significant reduction in CPK peak time [(19.4±6.5)h vs.(13.9±4.5)h ]and CK-MB peak time [(19.7±7.7)h vs.(12.7±7.2) h]in direct PCI group,P <0.01 all;there were significant reductions in cardiac rupture rate (5.8% vs.0),re-in-farction rate (17.5% vs.6.3%)during hospitalization;mortality rates within 30d (11.0% vs.2.2%)and one year (22.2% vs.8.2%)in direct PCI group,P <0.05 all.Conclusion:Direct PCI in STEMI patients possesses better therapeutic effect and safety,which is worthy extending.

Aged ; Diagnosis, Differential ; Humans ; Intermittent Claudication ; diagnosis ; etiology ; Male ; Middle Aged ; Nervous System Diseases ; complications ; diagnosis ; Vascular Diseases ; complications ; diagnosis

Objective To explore the effects of implanting different diameter single hair in treatment of part eyebrow defect.Methods 49 patients with part eyebrow defect,including 37 congenital sparse eyebrows and 12 traumatic eyebrow detect,were performed hair transplantation.Single-hair grafts were harvested from the scalp behind ear or the inferior part of the occiput.Single-hair grafts were implanted according to directions of normal eyebrow.22G or 23G needle was used for stick-andplace insertion.The thick and thin hairs were divided.The thin hairs were implanted into the medial and distal ends and the upper edge,the thick hairs were implanted into the center.Results After 3-10 months follow-up.The reconstructed eyebrows growed slowly,about every 5-7 days needed be trimmed.Two sides eyebrows were symmetry.Because a small amount of transplanted hairs were not alive,twelve patients were performed the second operation for thicker eyebrow.All patients were satisfied with the results.Conclusions In treatment of part eyebrow defect,the results of implanting different diameter single hair are natural and vivid.

Continuous progress of medicine and related areas are initiating and motivating a paradigm transformation of traditional surgery to precise surgery,which is characterized by precision in decision making and surgical intervention.The strategy of precise surgery is to seek a balance of maximized lesion removal,maximized organ sparing and minimal surgical invasiveness.Due to the special location and biological characteristics,the therapy of hilar cholangiocarcinoma is still challenging.To meet the demand of precise surgery,the knowledge of anatomy,biological characteristics and liver functional reserve is needed and the technical aspects of pre-surgical intervention,liver resection and reconstruction of vessels are also very important.

AIM: The aim of the present study was to clarify the mechanism of intracellular signal transduction in Ang Ⅱ- induced proliferation of vascular smooth muscular cells (VSMC) by observing the effect of c- Src on Ang Ⅱ- mediated mitogen- activated protein kinase (MAPK) activation and c- Fos protein expression in cultured VSMC of rats. METHODS: Cultured aortic VSMCs from SD rats were transfected with anti-sense c-Src oligodeoxynucleotides (ODNs) wrapt with lipofectin to inhibit c- Src activity and protein production. Untransfected VSMCs were used as control. We observed the role of Ang Ⅱ stimulation in MAPK activation and c- Fos protein expression. c- Src kinase activity was measured by protein immunoprecipitation and kinase autophosphorylation. The phosphorylation rate of the substrate myelin basic protein (MBP) was employed to assess MAPK activity.Western immunoblot was used to detect protein expression of c- Src and c-Fos. RESULTS: c-Src protein expression in VSMC transfected with different concentrations of anti- sense ODNs significantly decreased in a negative dose- effect manner. c- Src kinase activity was also markedly inhibited. Following the stimulation of Ang Ⅱ on transfected VSMCs with anti-sense ODNs, the increase rate of c- Src activity was 8.7% of that in control, the activity of MAPK was 1.6% compared with control and c- Fos protein expression was as 30.0% as that of control. CONCLUSION: Ang Ⅱ induces c- Src activation. MAPK activation and c - Fos protein expression by Ang Ⅱ is dependent on c- Src activation. These findings indicate that c- Src is an important signal factor in Ang Ⅱ-induced VSMC proliferation.

OBJECTIVE:To establish an HPLC method for the determination of Icariine in Qianlietong tablets.METHODS:Hypersil ODS(200 mm?4.6 mm,5 ?m) column was used for the HPLC determination,and the mobile phase consisted of acetonitrile-water(30∶70) at a flow rate of 1.0 mL?min-1.The detection wavelength was 270 nm.RESULTS:Icariine has good linear relationship in the range of 0.050 6～0.506 0 ?g(r=0.999 9).The average recovery was 102.67%(RSD=0.99%,n=6).CONCLUSION:The established method is simple,reproducible,specific and suitable for the quality control of Qianlietong tablets.