Based on the requirement of biology education development,the article analyzes the approach to improve the teaching quality of medical biology from quality education,subjiect intercrossing or 'regression' and 'outspread',and offers new ideas for education reform and innovation of the medical biology.

Acetates ; urine ; Chromatography, Gas ; Gas Chromatography-Mass Spectrometry ; Humans

In recent years, various studies and analyses related to cosmetics safety were conducted according to routine tests in Hygienic Standard of Cosmetics (2007), and the eligible rates of tested cosmetics were high. But the other prohibited and limited use components, such as antibiotics were analyzed rarely, and meanwhile some kinds of cosmetic related dermatitis cases appeared dramatically. Several dermatitis, especially contact dermatitis and hormone dependent dermatitis symptoms were not contained in Diagnostic Criteria and Principles of Management of Skin Diseases Induced by Cosmetics-General Guideline, GB 17149.1—1997. So it indicated the standard, GB 17149.1—1997 should be revised and some prohibited and limited use components such as hormone and antibiotic testing should be appended to the safety analysis of cosmetics.

BACKGROUND: Many studies focus on transforming growth factor β (TGF β) and its receptors, however, the distdbution of type Ⅰ TGF receptor (TGF-βR Ⅰ) in peripheral region of hypertrophic scars remain poorly understood. OBJECTIVE: To determine the expression and distribution of TGF-βR Ⅰ and type Ⅰ collagen in the peripheral and central areas of human skin hypertrophic scar. METHODS: A total of 30 cases with human cutaneous scars admitted at the Department of Plastic Surgery, First Affiliated Hospital and Department of Mammary Gland, Head and Neck Surgery, Tumor Hospital of Xinjiang Medical University from 1999 to 2002, were selected, including 20 cases with hypertrophic scar and 10 cases with normal scars. A total of 180 scars were obtained from central and peripheral areas of scars as well as normal skin tissues. The protein contents of TGF-βR1 and type Ⅰcollagen was detected by immunohistochemistry. In addition, the immunostaining positive in these samples was analyzed by semiquantitative analysis. RESULTS AND CONCLUSION: Compared to non hypertrophic scar and normal skin tissues, the TGF-βR1 expression of hypertrophic scar was obvious greater with strong positive reaction. The TGF-β R Ⅰ content was 100% in peripheral region of hypertrophic scar, which was notably 20% greater than that of central area (P < 0.05). The content of type Ⅰ collagen was both 100% in peripheral and central areas. The differences of positive TGF-β R Ⅰ and type Ⅰ collagen had no significance between peripheral and central areas of non hypertrophic scars (P > 0.05). There were few contents of TGF-βR Ⅰ and type Ⅰ collagen in normal skin tissues. The expression of TGF-β R Ⅰ is higher in peripheral than central areas of hypertrophic scar. Therefore, the peripheral area would be emphasized in the clinic work.

We found that the number of institutions made use of the undocumented in vitro diagnostic reagent in the survey. The phenomenon poses some risks and problems. In use this paper, we analyzed the situation and the reasons for the use of the undocumented in vitro diagnostic reagents, and put forward the corresponding measures.
Humans ; Indicators and Reagents ; standards

OBJECTIVE: To evaluate the similarity of the mean drug dissolution or release curves by the experimental model of reformatory Weibull. METHODS: Three drugs were taken as the model drugs. With the fiber-optic in site dissolution testing equipment(FODT), the percentages of accumulated dissolution/release changing with time of the control and test preparations of every drug were respectively monitored. The mean percentages were calculated and the dissolution/release curves were drawn. The data were fitted by the reformatory Weibull model. The fitted parameters c, Ti, b, a, and the 95% confidence intervals of every parameters were extracted. The similarity of the mean drug dissolution curves between the control and test preparations was evaluated by the fitted parameters and observation of the dissolution curves. RESULTS: For the sustained-release preparations, there was no significant difference in the parameters c, Ti, b, and a of the test and control samples, and the mean drug release curves were similar. As for the non-modified preparations, whether the dosage forms of the test and control preparations were the same or not, there existed differences in the parameters fitted by the method of reformatory Weibull model. CONCLUSION: The modified method of reformatory Weibull model can be applied in the evaluation of similarity of drug dissolution behavior with satisfactory goodness-of-fit and quantitative result.

Objective To observe the effects of early rehabilitation training on serum brain-derived neurotrophic factor (BDNF) expressionand motor function in patients with acute cerebral infarction. Methods 48 patients with acute cerebral infarction were randomly dividedinto rehabilitation group (n=24) and control group (n=24). The control group accepted routine medication, while the rehabilitation group acceptedearly rehabilitation training in addition. They were assessed with Fugl-Meyer Assessment (FMA), and the expression of BDNF in serumwas detected before and after treatment. Results The expression of BDNF and the score of FMA increased significantly after treatmentin both groups (P<0.05), but increased more in the rehabilitation group than in the control group (P<0.05). Conclusion Early rehabilitationtraining can promote the expression of serum BDNF and recovery of motor function in patients with acute cerebral infarction.

Objective:To explore the inhibition of microRNA (miRNA, miR)-5590-3p on the expression of transforming growth factor beta typeⅡreceptor (TGFBR2) gene and its effect on the invasion and proliferation of gastric cancer cell line HS-746T.Methods:The gastric cancer cell line was cultured in vitro. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to analyze the expression of miR-5590-3p in gastric cancer tissues and cell lines. The miR-NC and miR-5590-3p mimic were transfected into gastric cancer cell line HS-746T by lipofection, and named as miR-NC group and miR-5590-3p group, respectively. qRT-PCR was used to measure transfection effects. Transwell assay and cell counting kit-8 (CCK-8) assay were used to detect cell invasion and proliferation after transfection. The bioinformatics software predicts and the dual luciferase reporter gene system validates the target gene of miR-5590-3p. qRT-PCR and western blot were used to measure the expression of TGFBR2 and its downstream proteins in the transfected cells. Results:The expression of miR-5590-3p in gastric cancer tissues was significantly lower than that in adjacent tissues ( P<0.01). The expression of miR-5590-3p in gastric cancer cell lines was significantly lower than that in normal gastric mucosal epithelial cells ( P<0.05), and lowest in HS-746T cells ( P<0.01). After transfection, the expression of miR-5590-3p in miR-5590-3p group (11.76±0.21) was significantly higher than that in miR-NC group (1.06±0.21), with statistically significant difference ( P<0.01). The number of invasive cells in miR-NC group and miR-5590-3p group were (101.20±15.47) and (26.53±6.53), respectively, and the invasion ability of miR-5590-3p group was significantly decreased ( P<0.01). Compared with the miR-NC group, the cell proliferation ability of the miR-5590-3p group was significantly decreased ( P<0.05). Bioinformatics software showed that the target gene for miR-5590-3p is TGFBR2. The dual luciferase reporter gene system confirmed that miR-5590-3p can target the TGFBR2 gene ( P<0.01). Western blot results showed that compared with miR-NC group, the expression of TGFBR2 in HS-746T cells in miR-5590-3p group was significantly decreased ( P<0.01), the expression of ZEB-1 and ZEB-2, and the expression of CDK1 and cyclin B proteins were decreased as well. Conclusions:miR-5590-3p can inhibit the invasion and proliferation of gastric cancer cell HS-746T by targeting and regulating the expression of TGFBR2 gene.

The silent cerebral infarct is an clinic symptom that is so slight or transitory as to be easily neglected. There are only neural symptoms and signs,but irresponsible infarct focus can be seen on image. The risk factors of silent cerebral infarct may be the same as those of symptomatic cerebral infarct. Such infarct is likely to influence the course, clinic symptom and prognosis of acute cerebral infarct and may foretell symptomatic cerebral infarct and deteriorate into pseudo-global paralysis or multi-infarcted dementia. Therefore elder who suffer from hypertension or diabetes and experience vertigo, headache, language barrier but without apparent signs and symptoms should receive cerebral CT or MRI. Minor or third-degree precautionary measures can be taken as a chief therapy for cerebral infarct. Alternatively vitamines B_6,B_(12) and folic acid can be supplied to reduce the chance of hyperhomocysteinemia. Headache is the initial symptom in silent brain infarct. Magnesium is effective when dehydration does not work.

Objective To investigate the relationship between Kashin-Beck Disease(KBD) and Human Parvovirus B19(HPVB19).Methods HPVB19DNA was detected in 55 sera of KBD patients and 52 healthy in adjacent non-endemic area and 35 healthy sera in normal area using PCR and then linked the HPVB19DNA to pGEM-T vector.The nucleotide sequence was analyzed and compared with HPVB19 nucleotide sequence published by Genebank and another in Journal of virology.Results HPVB19DNA was found in 16 of 55 sera in KBD patients,and the HPVB19DNA position rate(29.09%) is significantly higher than that of the two healthy control groups(11.54%、11.42% respectively)(P＜0.05).The nucleotide sequence homologies compared with the two published nucleotide sequence were 97.75%、97%,respectively.The putative amino acid homologies compared with the tow published were 93.5%.The amino acid variation was greater than the nucleotide sequence variation because of a base insertion.Conclusion There was a close relationship between HPVB19 infection and Kashin-Beck Disease.