Objective To investigate the mechanism of preconditioned neuroprotection of cerebellar fastigial nucleus electrical stimulation(FNS) by means of observation of the effect of FNS on the expressions of PKC? and PKC? in the somatosensery cortex and basal ganglia in rats. Methods FNS models in rats were established. Brain tissue containing the somatosensery cortex and basal ganglia was removed at 0 h and 1, 3, 7 and 10 d respectively after FNS and 20 ?m coronal sections were obtained with a sliding cryo microtome. Immunocytochemical analysis of PKC? and PKC? in 2 sections from each rat was performed and the average optical density of immunostaining was quantified by computer assisted image analysis system. Sham stimulation rats and cerebral dentate nucleus (DN) stimulation rats were used as the control. Results At 0 h after FNS, no significant changes of the expressions of PKC? and PKC? were found in the contralateral somatosensery cortex and basal ganglia. The expressions of PKC? and PKC? increased significantly at 1 d and decreased at 3 d but were still higher than those in sham stimulation group at 7 d and decreased to the basal level of the control at 10 d. However, the expressions of PKC? and PKC? in the ipsilateral somatosensery cortex and basal ganglia were also elevated at 1 d after FNS but the increase was significantly lower than that in the contralateral ones and reached the basal level at 3 d. There was no change of PKC? and PKC? in sham stimulation group at 1 d after DN stimulation. Conclusion The increased expressions of PKC? and PKC? in the somatosensery cortex and basal ganglia induced by FNS may participate in the preconditioned neuroprotection of FNS.

OBJECTEVE:To probe into the difference of drug prices between used in hospital and retailed in market.METHODS:Analysis the different reasons of drug price from drug quality assurance system,pharmaceutical care,drug using structure system and function mechanism.RESULTS&CONCLUSION:It's rational that the drug prices used in hospital high_ er than in retailed market.It's pratical and realistic attitude to the fact that the same drug has different prices in the medical market.

Objective: To evaluate the application value of large flat-panel multifunction digital machine in digestive system. Methods: Exam the gastrointestinal tract and biliary with dynamic detector, and then observe its advantages and disadvantages. Results: All the 13 gastrointestinal cancer lesions and 15 inflammatory lesions were shown clearly, including mucosa. Biliary tomography found two small stones, which were undetected by the general X-ray photography. The excellent rate of the photo was improved to 98%. Conclusion: The advantages of the flat panel detector include the wide range of display, clear image of digital pulse fluoroscopy spot film, the improvement of the diagnostic accuracy and the reduction in radiation dose. The disadvantage is that it is more inconvenient than the remote cradle gastrointestinal machine in rotating the position of the patient.

Aged ; Antibodies, Monoclonal, Murine-Derived ; therapeutic use ; Antigens, CD20 ; metabolism ; Antineoplastic Agents ; therapeutic use ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Cyclophosphamide ; therapeutic use ; Doxorubicin ; therapeutic use ; Female ; Follow-Up Studies ; Humans ; Lymphoma, Large B-Cell, Diffuse ; drug therapy ; metabolism ; pathology ; surgery ; Neoplasm Invasiveness ; Prednisone ; therapeutic use ; Proto-Oncogene Proteins c-bcl-2 ; metabolism ; Proto-Oncogene Proteins c-bcl-6 ; metabolism ; Rituximab ; Uterine Neoplasms ; drug therapy ; metabolism ; pathology ; surgery ; Vincristine ; therapeutic use

Objective To evaluate the feasibility of blind intubation via Cookgas intubating laryngeal airway (CILA) in patients under general anesthesia. Methods Sixty ASA Ⅰ or Ⅱ patients aged 11-65 yrs scheduled for elective plastic surgery under general anesthesia were randomly allocated into 2 groups ( n = 30 each): blind intubation group (B) and fiberoptic bronchoscope group (FOB) . All patients were evaluated before operation to identify patients with difficult airway. Anesthesia was induced with midazolam 0.05 mg?kg-1 , fentanyl 2 ?g?kg-1 , propofol 2 mg?kg-1 and vecuronium 0.1 mg?kg-1 . CILA was inserted in all patients. The patients in group B were blindly intubated via CILA, while in group FOB tracheal intubation was guided with fiberoptic bronchoscope via CILA. The time spent in intubation and the success rate of tracheal intubation were recorded. SBP, DBP, HR and SpO2 were recorded immediately before and after induction of anesthesia, immediately after insertion of CILA, immediately after tracheal intubation and immediately after CILA was withdrawn. Results CILA was inserted successfully in all patients. In group B tracheal intubation was accomplished at first attempt in 26 patients, at 2nd or 3rd attempt in 2 patients and failed in 2 patients. In group FOB tracheal intubation was much easier and accomplished at 1st attempt in 29 patients and at 2nd attempt in 1 patient. Twenty four of 25 patients expected to be difficult intubation were intubated successfully. The two groups were comparable with respect to hemodynamic changes during intubation. Conclusion It is possible to intubate via CILA. The success rate is high with minimal cardiovascular response.

Objective To explore the prenatal ultrasonographic features of fetuses diagnosed as echogenic bowel and chromosomal abnormalities. Methods From September 2009 to June 2013, eighty cases diagnosed as echogenic bowel by prenatal ultrasound screening in our hospitals underwent chromosomal karyotype analysis and were followed up till fetal birth. The prenatal ultrasonographic features were carefully correlated with the postnatal findings. Results Prenatal ultrasoundand chromosomal findings of the 80 cases were:(1) Fifty-eight cases of fetuses with echogenic bowel alone (72.5%, 58/80, without chromosomal karyotype abnormalities). (2) Eleven cases of fetuses with additional ultrasound soft markers (13.8%, 11/80, without chromosomal karyotype abnormalities). The ultrasound examination revealed 8 cases of choroid plexus cysts, 1 case of single umbilical artery, 1 case of shorter nasal bone, and 1 case of echogenic intracardiac focus. (3) Five cases of fetuses with severe functional abnormalities or complex structural malformations and normal chromosomal karyotype. The ultrasound examination revealed 3 cases of structural cardiac malformations, 1 case of heart failure and 2 cases of central nervous system malformations. Of them,one case showed complex malformations (right ventricular dysplasia syndrome, cerebellar dysplasia and single umbilical artery), whereas the remaining 4 cases only involve single malformation. (4)Six cases had chromosomal abnormalities (7.5%, 6/80), including 3 cases of abnormal chromosome microdeletions, and 3 cases of abnormal chromosome numbers (1 21-trisomy syndrome, 1 18-trisomy syndrome, and 1 triploid syndrome). All of them had severe structural malformations and abnormal soft markers. The ultrasound examination revealed 2 cases of severe cardiac malformations, 2 cases of central nervous system malformations, 1 case of pericardial/peritoneal effusion, and 1 case of placental chorionic multiple hemangioma. One case of holoprosencephaly (donor of the twins) ended with intrauterine death. The associated abnormal soft markers include nuchal fold (NF) thickening, choroid plexus cysts, umbilical cord cysts, single umbilical artery, and placental chorionic hemangioma. Conclusions The fetuses with echogenic bowelaloneusualyhaveagoodprognosis.Insomecases,echogenicbowelmaydisappearduringvfolow-up.The fetuses complicated with severe structural malformations and chromosomal abnormalities have poor prognosis. For the fetuses diagnosed of echogenic bowel by prenatal ultrasound screening, systematic ultrasound examination should be carried out and clinical chromosomal karyotype analysis was suggested.

Objective This research examined the effect ofmicrobubble contrast agent plus ultrasound on the permeability of blood-brain barrier,and explored whether it affects the efficacy of chemotherapeutic drugs on cerebral glioblastoma.Methods Wistar rats were divided into three groups to find the optimal concentration of ultrasonic contrast agent.To identify the best ultrasound mode that affected the permeability of blood brain barrier,we employed transmission electron microscopy for study of brain ultrastructure.Western blotting was used to detect the tight junction protein claudin-5.Evans blue staining of brain tissues was utilized to identify the best ultrasonic contrast agent concentration and mode.Rat glioma cells (line 9L) were injected into Wistar rats.After temozolomide chemotherapy,the tumor size was measured and the tumor marker GFAP in serum was detected by ELISA.Results The best contrast agent concentration which increases permeability of BBB in rats was found to be lml/kg and the best ultrasound mode was intermittentlytriggered pulses lasting for 10min (with interval was set at 400ms).More Evans blue passed the blood-brain barrier in ultrasonic cavitation effect group than in control group (P＜0.05).After temozolomide chemotherapy,more tumor marker GFAP was detected in ultrasonic cavitation effect group than in control group (P＜0.05).Conclusion The permeability of BBB was increased and more temozolomide went through BBB when the rats were subjected to intermittently triggered ultrasonic pulses and were injected at contrast agent at lml/kg,which could help to achieve better therapeutic efficacy for glioblastoma.

Primary hypertension, known as one of the most common diseases of the time, is thought to be determined by the combined effect of several risk factors and stages, especially for the interaction of genetic and environmental factors. However, the mechanisms of genetic and environmental factors leading to the increased blood pressure are still not clear. Previous studies appear that Chinese materia medica (CMM) compound shows a good effect in the treatment of hypertension, its mechanisms for the action are mainly related to the activation of the rennin-angiotensin-aldosterone system (RAAS), calcium antagonists, blood vessels dilation, the improvement of endothelial function, hemorheology, and insulin resistance. It may also relate to diuretic, the regulation of the blood lipids, AR, ER alpha, and the loss of weight, etc. Therefore, this review summarizes the research progress at home and abroad on the mechanisms of CMM compound in the treatment of hypertension in recent years, aiming to provide information for the clinical treatment of hypertension.

Objective To observe the characteristics of changes in tissue oxygen partial pressure in Zusanli (ST36) acupoint and related organs in acute respiratory distress syndrome (ARDS), and to clarify the correlation between oxygenation of local tissue and systemic oxygenation and the correlation between oxygen metabolism of acupoint tissues and related organ tissues.Methods Twenty healthy New Zealand white rabbits were selected and divided into an ARDS model group and a control group, according to random number table method, 10 in each group. Oleic acid (0.08 - 0.1 mL/kg) intravenous injection was used to replicate the ARDS animal model. Only tracheotomy, mechanical ventilation, insertion of arterial/venous catheter, etc other manipulations were conducted, and no oleic acid was injected in the control group. Tissue oximeter was used to determine the fraction of inspired oxygen (FiO2), when FiO2 was 0.21 and 1.00 respectively, the tissue oxygen partial pressure (PtO2) in Zusanli acupoint, stomach and liver was measured. Meanwhile, the blood gas analyses of arterial blood and mixed venous blood were carried out to calculate the oxygen extraction rate (O2ER).Results When FiO2 was equal to 0.21, the levels of PtO2 in Zusanli acupoint, stomach and liver of the ARDS model group were significantly higher than those of the control group in the same period; the partial pressure of oxygen arterial blood (PaO2), partial pressure of oxygen in mixed venous blood (PvO2), arterial oxygen saturation (SaO2), mixed venous oxygen saturation (SvO2) and O2ER of the ARDS model group were significantly lower than those of the control group over the same period. When FiO2 was equal to 1.00, PtO2, PaO2, SaO2 and O2ER in Zusanli acupoint, stomach and liver were increased compared with those when FiO2 was 0.21 in both groups, and the increase of PtO2 and O2ER in Zusanli acupoint, stomach and liver was more significant in the ARDS model group [PtO2 (kPa): Zusanli acupoint: 16.75±2.12 vs. 13.80±1.83, stomach: 16.45±1.33 vs. 13.35±1.25, liver: 16.43±1.45 vs. 13.45±1.36, O2ER: (36.14±0.97)% vs. (30.81±1.01)%]; the increase of PaO2, SaO2 and SvO2 was more significant in the control group [PaO2 (mmHg, 1 mmHg = 0.133 kPa): 682.02±50.32 vs. 159.32±40.17, SaO2: 1.00±0.00 vs. 0.98±0.01, SvO2: 0.69±0.01 vs. 0.63±0.03, allP < 0.05]. The indexes under FiO2 0.21 compared to those under FiO2 1.00 in the same group, it was shown that when FiO2 1.00, PvO2 was increased in the ARDS model group, but decreased in the control group, the difference between the two groups being statistically significant (mmHg: 36.00±2.83 vs. 42.50±1.70,P < 0.05). Besides, PtO2 in Zusanli acupoint was positively correlated to that in stomach (r = 0.963,P < 0.001).Conclusions When ARDS is under the condition of systemic oxygen delivery disorder, the demand of organ tissues for oxygen is significantly increased, but the oxygen utilization is impaired obviously, which is possibly related to the cellular mitochondrial dysfunction. There is good correlation between the tissue oxygen partial pressure of acupoint tissue and related organ tissue. The detection of oxygen in acupoint tissue has important guidance significance for monitoring the oxygen metabolism of related organ tissues.

Objective To evaluate the relationship between the cervical length,the test of fetal fibronectin and premature birth in pregnant woman.Methods Fetal fibronectin in sample of cervical secretion was tested using the reagent box of FFN manufactured by the ADEIA company of the United States and enzyme-labeled immunosorbent assay(ELISA) methods in 110 pregnant women.They were divided into two groups:first group,there were 60 cases who had symptom of aura prematurity and cervical lengths ≤30 mm monitored by trans-perineum ultrasound.second group was 50 normal pregnant women.Results The postive rate of fetal fibronectin was higher significantly in first group than that in second group.Conclusion The measurement of cervical lengths by the trans-perineum ultrasound and the test of Fetal fibronectin in pregnant women who has symptom of aura prematurity are of certain clinical value in predicting preterm birth.